PE/Cyanine5 anti-mouse I-A/I-E | BioLegend 107612 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Cyanine5 anti-mouse I-A/I-E

catalog :

107612

quantity :

100 μg

price :

230 USD

clonality :

monoclonal

host :

rat

conjugate :

PE-Cy5

clone name :

M5/114.15.2

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 2	McDonnell A, Lesterhuis W, Khong A, Nowak A, Lake R, Currie A, et al. Tumor-infiltrating dendritic cells exhibit defective cross-presentation of tumor antigens, but is reversed by chemotherapy. Eur J Immunol. 2015;45:49-59 pubmed publisher
	Youshani A, Rowlston S, O Leary C, Forte G, Parker H, Liao A, et al. Non-myeloablative busulfan chimeric mouse models are less pro-inflammatory than head-shielded irradiation for studying immune cell interactions in brain tumours. J Neuroinflammation. 2019;16:25 pubmed publisher
	Fu H, Kishore M, Gittens B, Wang G, Coe D, Komarowska I, et al. Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation. Nat Commun. 2014;5:3436 pubmed publisher
	Dráber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, et al. SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011;31:4550-62 pubmed publisher
	Sabatino J, Huang J, Zhu C, Evavold B. High prevalence of low affinity peptide-MHC II tetramer-negative effectors during polyclonal CD4+ T cell responses. J Exp Med. 2011;208:81-90 pubmed publisher
	Gravano D, McLelland B, Horiuchi K, Manilay J. ADAM17 deletion in thymic epithelial cells alters aire expression without affecting T cell developmental progression. PLoS ONE. 2010;5:e13528 pubmed publisher
	Kuns R, Morris E, MacDonald K, Markey K, Morris H, Raffelt N, et al. Invariant natural killer T cell-natural killer cell interactions dictate transplantation outcome after alpha-galactosylceramide administration. Blood. 2009;113:5999-6010 pubmed publisher
	De Pascalis R, Taylor B, Elkins K. Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain. Infect Immun. 2008;76:4311-21 pubmed publisher
	Matte Martone C, Liu J, Jain D, McNiff J, Shlomchik W. CD8+ but not CD4+ T cells require cognate interactions with target tissues to mediate GVHD across only minor H antigens, whereas both CD4+ and CD8+ T cells require direct leukemic contact to mediate GVL. Blood. 2008;111:3884-92 pubmed publisher
	Christensen S, Shupe J, Nickerson K, Kashgarian M, Flavell R, Shlomchik M. Toll-like receptor 7 and TLR9 dictate autoantibody specificity and have opposing inflammatory and regulatory roles in a murine model of lupus. Immunity. 2006;25:417-28 pubmed

product information

Antigen :

I-A/I-E

Apps. Abbrev. :

Flow Cytometry

Cat # :

107612

Clone :

M5/114.15.2

Item :

PE/Cyanine5 anti-mouse I-A/I-E

Isotype :

Rat IgG2b, κ

Other Names :

MHC class II

Size :

100 μg

Price (USD) :

230 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PE_CYANINE5

Immunogen :

Activated C57BL/6 mouse spleen cells

Application Notes :

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A b , I-A d , I-A q , I-E d , and I-E k MHC class II alloantigens from mice carrying H-2 p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A f , I-A k , or I-A s MHC class II alloantigens. 1 Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemistry of frozen sections 2,3,6 , and in vitro and in vivo blocking of antigen presentation or ligand binding 4-7 . The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 107655 107656).

more info or order :

BioLegend product webpage