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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse I-A/I-E
catalog :
107608
quantity :
200 μg
price :
270 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
M5/114.15.2
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 31
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig ex4o
Culemann S, Grüneboom A, Nicolás Ávila J, Weidner D, Lämmle K, Rothe T, et al. Locally renewing resident synovial macrophages provide a protective barrier for the joint. Nature. 2019;572:670-675 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
  • flow cytometry; mouse; fig 3c
Stoilova B, Kowenz Leutz E, Scheller M, Leutz A. Lymphoid to myeloid cell trans-differentiation is determined by C/EBP? structure and post-translational modifications. PLoS ONE. 2013;8:e65169 pubmed publisher
Cai W, Kesavan D, Cheng J, Vasudevan A, Wang H, Wan J, et al. Vesicle-Mediated Dendritic Cell Activation in Acinetobacter baumannii Clinical Isolate, which Contributes to Th2 Response. J Immunol Res. 2019;2019:2835256 pubmed publisher
Koyama M, Mukhopadhyay P, Schuster I, Henden A, Hülsdünker J, Varelias A, et al. MHC Class II Antigen Presentation by the Intestinal Epithelium Initiates Graft-versus-Host Disease and Is Influenced by the Microbiota. Immunity. 2019;51:885-898.e7 pubmed publisher
Shi L, Sheng J, Wang M, Luo H, Zhu J, Zhang B, et al. Combination Therapy of TGF-β Blockade and Commensal-derived Probiotics Provides Enhanced Antitumor Immune Response and Tumor Suppression. Theranostics. 2019;9:4115-4129 pubmed publisher
Garris C, Arlauckas S, Kohler R, Trefny M, Garren S, Piot C, et al. Successful Anti-PD-1 Cancer Immunotherapy Requires T Cell-Dendritic Cell Crosstalk Involving the Cytokines IFN-γ and IL-12. Immunity. 2018;49:1148-1161.e7 pubmed publisher
Biton M, Haber A, Rogel N, Burgin G, Beyaz S, Schnell A, et al. T Helper Cell Cytokines Modulate Intestinal Stem Cell Renewal and Differentiation. Cell. 2018;175:1307-1320.e22 pubmed publisher
Porrello A, Leslie P, Harrison E, Gorentla B, Kattula S, Ghosh S, et al. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking. Nat Commun. 2018;9:1988 pubmed publisher
Dhandapani R, Arokiaraj C, Taberner F, Pacifico P, Raja S, Nocchi L, et al. Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons. Nat Commun. 2018;9:1640 pubmed publisher
Lin S, Hsieh S, Fan Y, Wei W, Hsiao P, Tsai D, et al. Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance. Autophagy. 2018;14:778-795 pubmed publisher
Jenkins R, Aref A, Lizotte P, Ivanova E, Stinson S, Zhou C, et al. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids. Cancer Discov. 2018;8:196-215 pubmed publisher
Platteel A, Henri S, Zaiss D, Sijts A. Dissecting antigen processing and presentation routes in dermal vaccination strategies. Vaccine. 2017;35:7057-7063 pubmed publisher
Sanchez M, Kolar S, Müller S, Reyes C, Wolf A, Ogawa C, et al. O-Acetylation of Peptidoglycan Limits Helper T Cell Priming and Permits Staphylococcus aureus Reinfection. Cell Host Microbe. 2017;22:543-551.e4 pubmed publisher
Espinosa Cueto P, Magallanes Puebla A, Castellanos C, Mancilla R. Dendritic cells that phagocytose apoptotic macrophages loaded with mycobacterial antigens activate CD8 T cells via cross-presentation. PLoS ONE. 2017;12:e0182126 pubmed publisher
Galani I, Triantafyllia V, Eleminiadou E, Koltsida O, Stavropoulos A, Manioudaki M, et al. Interferon-? Mediates Non-redundant Front-Line Antiviral Protection against Influenza Virus Infection without Compromising Host Fitness. Immunity. 2017;46:875-890.e6 pubmed publisher
Novković M, Onder L, Cupovic J, Abe J, Bomze D, Cremasco V, et al. Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality. PLoS Biol. 2016;14:e1002515 pubmed publisher
Zhu J, Zhang Y, Zhang A, He K, Liu P, Xu L. Cryo-thermal therapy elicits potent anti-tumor immunity by inducing extracellular Hsp70-dependent MDSC differentiation. Sci Rep. 2016;6:27136 pubmed publisher
Fontana C, Conde Álvarez R, Ståhle J, Holst O, Iriarte M, Zhao Y, et al. Structural Studies of Lipopolysaccharide-defective Mutants from Brucella melitensis Identify a Core Oligosaccharide Critical in Virulence. J Biol Chem. 2016;291:7727-41 pubmed publisher
Pazmandi K, Agod Z, Kumar B, Szabo A, Fekete T, Sogor V, et al. Oxidative modification enhances the immunostimulatory effects of extracellular mitochondrial DNA on plasmacytoid dendritic cells. Free Radic Biol Med. 2014;77:281-90 pubmed publisher
Sakai F, Hosoya T, Ono Ohmachi A, Ukibe K, Ogawa A, Moriya T, et al. Lactobacillus gasseri SBT2055 induces TGF-β expression in dendritic cells and activates TLR2 signal to produce IgA in the small intestine. PLoS ONE. 2014;9:e105370 pubmed publisher
Markey K, Koyama M, Gartlan K, Leveque L, Kuns R, Lineburg K, et al. Cross-dressing by donor dendritic cells after allogeneic bone marrow transplantation contributes to formation of the immunological synapse and maximizes responses to indirectly presented antigen. J Immunol. 2014;192:5426-33 pubmed publisher
Fu H, Kishore M, Gittens B, Wang G, Coe D, Komarowska I, et al. Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation. Nat Commun. 2014;5:3436 pubmed publisher
Yasmin N, Bauer T, Modak M, Wagner K, Schuster C, Köffel R, et al. Identification of bone morphogenetic protein 7 (BMP7) as an instructive factor for human epidermal Langerhans cell differentiation. J Exp Med. 2013;210:2597-610 pubmed publisher
Dráber P, Vonkova I, Stepanek O, Hrdinka M, Kucova M, Skopcova T, et al. SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling. Mol Cell Biol. 2011;31:4550-62 pubmed publisher
Sabatino J, Huang J, Zhu C, Evavold B. High prevalence of low affinity peptide-MHC II tetramer-negative effectors during polyclonal CD4+ T cell responses. J Exp Med. 2011;208:81-90 pubmed publisher
Kuns R, Morris E, MacDonald K, Markey K, Morris H, Raffelt N, et al. Invariant natural killer T cell-natural killer cell interactions dictate transplantation outcome after alpha-galactosylceramide administration. Blood. 2009;113:5999-6010 pubmed publisher
De Pascalis R, Taylor B, Elkins K. Diverse myeloid and lymphoid cell subpopulations produce gamma interferon during early innate immune responses to Francisella tularensis live vaccine strain. Infect Immun. 2008;76:4311-21 pubmed publisher
Matte Martone C, Liu J, Jain D, McNiff J, Shlomchik W. CD8+ but not CD4+ T cells require cognate interactions with target tissues to mediate GVHD across only minor H antigens, whereas both CD4+ and CD8+ T cells require direct leukemic contact to mediate GVL. Blood. 2008;111:3884-92 pubmed publisher
Christensen S, Shupe J, Nickerson K, Kashgarian M, Flavell R, Shlomchik M. Toll-like receptor 7 and TLR9 dictate autoantibody specificity and have opposing inflammatory and regulatory roles in a murine model of lupus. Immunity. 2006;25:417-28 pubmed
product information
Antigen :
I-A/I-E
Apps. Abbrev. :
Flow Cytometry
Cat # :
107608
Clone :
M5/114.15.2
Item :
PE anti-mouse I-A/I-E
Isotype :
Rat IgG2b, κ
Other Names :
MHC class II
Size :
200 μg
Price (USD) :
270 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
Activated C57BL/6 mouse spleen cells
Application Notes :
The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A b , I-A d , I-A q , I-E d , and I-E k MHC class II alloantigens from mice carrying H-2 p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-A f , I-A k , or I-A s MHC class II alloantigens. 1 Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemistry of frozen sections 2,3,6 , and in vitro and in vivo blocking of antigen presentation or ligand binding 4-7 . The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 107655 107656).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA