Ultra-LEAF™ Purified anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

catalog :

107414

quantity :

50 mg

price :

2354 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

HK5.3

reactivity :

mouse

application :

neutralization, flow cytometry

more info or order :

BioLegend product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 1a, 1b	Angulo G, Železnjak J, Martínez Vicente P, Puñet Ortiz J, Hengel H, Messerle M, et al. Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion. elife. 2021;10: pubmed publisher
	Zhang Y, Chen A, Li D, Yuan Q, Zhu A, Deng J, et al. Development of T follicular helper cell-independent nanoparticle vaccines for SARS-CoV-2 or HIV-1 by targeting ICOSL. NPJ Vaccines. 2024;9:176 pubmed publisher
	Song J, Deshpande T, Zhang X, Hannocks M, Lycke N, Cardell S, et al. The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation. iScience. 2023;26:106753 pubmed publisher
	Rivera C, Randrian V, Richer W, Gerber Ferder Y, Delgado M, Chikina A, et al. Epithelial colonization by gut dendritic cells promotes their functional diversification. Immunity. 2022;55:129-144.e8 pubmed publisher
	Verma M, Michalec L, Sripada A, McKay J, Sirohi K, Verma D, et al. The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma. J Exp Med. 2021;218: pubmed publisher
	Bromley S, Akbaba H, Mani V, Mora Buch R, Chasse A, Sama A, et al. CD49a Regulates Cutaneous Resident Memory CD8+ T Cell Persistence and Response. Cell Rep. 2020;32:108085 pubmed publisher
	Zhang C, Yue C, Herrmann A, Song J, Egelston C, Wang T, et al. STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth. Cell Metab. 2020;31:148-161.e5 pubmed publisher
	Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher

product information

Antigen :

CD275

Apps. Abbrev. :

FC, Block

Cat # :

107414

Clone :

HK5.3

Item :

Ultra-LEAF™ Purified anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

Isotype :

Rat IgG2a, κ

Other Names :

ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L

Size :

50 mg

Price (USD) :

2354 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Immunogen :

Mouse B7-RP1 transfected cell line

Application Notes :

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding^1,2. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) (Endotoxin <0.01 EU/µg).;

more info or order :

BioLegend product webpage