PE anti-mouse CD275 (B7-RP1, ICOSL, B7H2) | BioLegend 107405 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

catalog :

107405

quantity :

50 μg

price :

121 USD

clonality :

monoclonal

host :

rat

conjugate :

clone name :

HK5.3

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 5e	Yan C, Saleh N, Yang J, Nebhan C, Vilgelm A, Reddy E, et al. Novel induction of CD40 expression by tumor cells with RAS/RAF/PI3K pathway inhibition augments response to checkpoint blockade. Mol Cancer. 2021;20:85 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a, 1b	Angulo G, Železnjak J, Martínez Vicente P, Puñet Ortiz J, Hengel H, Messerle M, et al. Cytomegalovirus restricts ICOSL expression on antigen-presenting cells disabling T cell co-stimulation and contributing to immune evasion. elife. 2021;10: pubmed publisher
flow cytometry; mouse; loading ...; fig s15d	Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
flow cytometry; mouse; loading ...; fig s1g	Binnewies M, Mujal A, Pollack J, Combes A, Hardison E, Barry K, et al. Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4+ T Cell Immunity. Cell. 2019;177:556-571.e16 pubmed publisher
	Molina M, Hoffman E, Stokes J, Kummet N, Smith K, Baker F, et al. Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death. Front Immunol. 2021;12:699128 pubmed publisher
	Zhang H, Dai D, Hu Q, Yang F, Xue Y, Li F, et al. Bach2 attenuates IL-2R signaling to control Treg homeostasis and Tfr development. Cell Rep. 2021;35:109096 pubmed publisher
	Lu Y, Zhao Q, Liao J, Song E, Xia Q, Pan J, et al. Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity. Cell. 2020;180:1081-1097.e24 pubmed publisher
	Mansouri S, Katikaneni D, Gogoi H, Pipkin M, Machuca T, Emtiazjoo A, et al. Lung IFNAR1hi TNFR2+ cDC2 promotes lung regulatory T cells induction and maintains lung mucosal tolerance at steady state. Mucosal Immunol. 2020;13:595-608 pubmed publisher
	Niven J, Madelon N, Page N, Caruso A, Harlé G, Lemeille S, et al. Macroautophagy in Dendritic Cells Controls the Homeostasis and Stability of Regulatory T Cells. Cell Rep. 2019;28:21-29.e6 pubmed publisher
	Zhou J, Peng H, Li K, Qu K, Wang B, Wu Y, et al. Liver-Resident NK Cells Control Antiviral Activity of Hepatic T Cells via the PD-1-PD-L1 Axis. Immunity. 2019;50:403-417.e4 pubmed publisher
	Barbet G, Sander L, Geswell M, Leonardi I, Cerutti A, Iliev I, et al. Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody Responses. Immunity. 2018;48:584-598.e5 pubmed publisher
	Lownik J, Luker A, Damle S, Cooley L, El Sayed R, Hutloff A, et al. ADAM10-Mediated ICOS Ligand Shedding on B Cells Is Necessary for Proper T Cell ICOS Regulation and T Follicular Helper Responses. J Immunol. 2017;199:2305-2315 pubmed publisher
	del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
	Zhang P, Sun D, Ke Y, Kaplan H, Shao H. The net effect of costimulatory blockers is dependent on the subset and activation status of the autoreactive T cells. J Immunol. 2007;178:474-9 pubmed

product information

Antigen :

CD275

Apps. Abbrev. :

Flow Cytometry

Cat # :

107405

Clone :

HK5.3

Item :

PE anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

Isotype :

Rat IgG2a, κ

Other Names :

ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L

Size :

50 μg

Price (USD) :

121 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

Immunogen :

Mouse B7-RP1 transfected cell line

Application Notes :

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding 1,2 . For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 107408). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).

more info or order :

BioLegend product webpage