Biotin anti-mouse CD275 (B7-RP1, ICOSL, B7H2) | BioLegend 107403 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

catalog :

107403

quantity :

50 μg

price :

95 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

HK5.3

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 6

Reference
Biram A, Winter E, Denton A, Zaretsky I, Dassa B, Bemark M, et al. B Cell Diversification Is Uncoupled from SAP-Mediated Selection Forces in Chronic Germinal Centers within Peyer's Patches. Cell Rep. 2020;30:1910-1922.e5 pubmed publisher
Yang J, Sakai J, Siddiqui S, Lee R, Ireland D, Verthelyi D, et al. IL-6 Impairs Vaccine Responses in Neonatal Mice. Front Immunol. 2018;9:3049 pubmed publisher
De Silva N, Silva K, Anderson M, Bhagat G, Klein U. Impairment of Mature B Cell Maintenance upon Combined Deletion of the Alternative NF-ÎºB Transcription Factors RELB and NF-ÎºB2 in B Cells. J Immunol. 2016;196:2591-601 pubmed publisher
White A, Jenkinson W, Cowan J, Parnell S, Bacon A, Jones N, et al. An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells. J Immunol. 2014;192:2659-66 pubmed publisher
del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
Zhang P, Sun D, Ke Y, Kaplan H, Shao H. The net effect of costimulatory blockers is dependent on the subset and activation status of the autoreactive T cells. J Immunol. 2007;178:474-9 pubmed

product information

Antigen :

CD275

Apps. Abbrev. :

Flow Cytometry

Cat # :

107403

Clone :

HK5.3

Item :

Biotin anti-mouse CD275 (B7-RP1, ICOSL, B7H2)

Isotype :

Rat IgG2a, κ

Other Names :

ICOS ligand, ICOSL, B7 homolog 2, B7-H2, B7H2, B7RP1, B7h, ICOS-L

Size :

50 μg

Price (USD) :

95 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

Mouse B7-RP1 transfected cell line

Application Notes :

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of ligand binding 1,2 . For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107405) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107404) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 107408). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 107410) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin 0.01 EU/µg).

more info or order :

BioLegend product webpage