Ultra-LEAF™ Purified anti-mouse CD154 | BioLegend 106516 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse CD154

catalog :

106516

quantity :

1 mg

price :

149 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

MR1

reactivity :

mouse

application :

neutralization, flow cytometry, immunohistochemistry - frozen section

more info or order :

BioLegend product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:600; loading ...; fig 2a	Koike T, Harada K, Horiuchi S, Kitamura D. The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. elife. 2019;8: pubmed publisher
	Gruber C, Calis J, Buta S, Evrony G, Martin J, Uhl S, et al. Complex Autoinflammatory Syndrome Unveils Fundamental Principles of JAK1 Kinase Transcriptional and Biochemical Function. Immunity. 2020;53:672-684.e11 pubmed publisher

product information

Antigen :

CD154

Apps. Abbrev. :

FC, IHC-F, Block

Cat # :

106516

Clone :

MR1

Item :

Ultra-LEAF™ Purified anti-mouse CD154

Isotype :

Armenian Hamster IgG

Other Names :

CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5

Size :

1 mg

Price (USD) :

149 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

ULEAF

Immunogen :

Activated mouse Th1 clone D1.6

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining^1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding^3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106515-106520).;

more info or order :

BioLegend product webpage