LEAF™ Purified anti-mouse CD154 | BioLegend 106508 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse CD154

catalog :

106508

quantity :

500 μg

price :

265 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

MR1

reactivity :

mouse

application :

neutralization, flow cytometry, immunohistochemistry - frozen section

citations: 5

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 2e	Atretkhany K, Mufazalov I, Dunst J, Kuchmiy A, Gogoleva V, Andruszewski D, et al. Intrinsic TNFR2 signaling in T regulatory cells provides protection in CNS autoimmunity. Proc Natl Acad Sci U S A. 2018;115:13051-13056 pubmed publisher
flow cytometry; mouse; 1:400; fig 6e	Singh M, Vianden C, Cantwell M, Dai Z, Xiao Z, Sharma M, et al. Intratumoral CD40 activation and checkpoint blockade induces T cell-mediated eradication of melanoma in the brain. Nat Commun. 2017;8:1447 pubmed publisher
flow cytometry; mouse; loading ...; fig 5a	Liu W, Kang S, Huang Z, Wu C, Jin H, Maine C, et al. A miR-155-Peli1-c-Rel pathway controls the generation and function of T follicular helper cells. J Exp Med. 2016;213:1901-19 pubmed publisher
flow cytometry; mouse; fig 4e	Parsa R, Lund H, Georgoudaki A, Zhang X, Ortlieb Guerreiro Cacais A, Grommisch D, et al. BAFF-secreting neutrophils drive plasma cell responses during emergency granulopoiesis. J Exp Med. 2016;213:1537-53 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 5e	Göbel K, Pankratz S, Asaridou C, Herrmann A, Bittner S, Merker M, et al. Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells. Nat Commun. 2016;7:11626 pubmed publisher

product information

Antigen :

CD154

Apps. Abbrev. :

Flow Cytometry, IHC-F, Block

Cat # :

106508

Clone :

MR1

Item :

LEAF™ Purified anti-mouse CD154

Isotype :

Armenian Hamster IgG

Other Names :

CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5

Size :

500 μg

Price (USD) :

265 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

LEAF

Immunogen :

Activated mouse Th1 clone D1.6

Application Notes :

Additional reported applications (for the relevant formats) include: immunohistochemical staining 1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding 3-5 . For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 106515-106520).