Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

PE/Cyanine5 anti-mouse CD152

106337

25 μg

147 USD

monoclonal

hamsters

nonconjugated

UC10-4B9

FC

106337

UC10-4B9

PE/Cyanine5 anti-mouse CD152

Armenian Hamster IgG

Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

25 μg

147 USD

Mouse

Monoclonal

Armenian Hamster

PE_CYANINE5

Mouse CTLA-4-mouse IgG2a fusion protein

The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation^1, in vitro stimulation, in vitro and in vivo blocking^1-4 of ligand binding, and as ELISA capture antibody^5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).;