TotalSeq™-B0388 anti-mouse CD152 | BioLegend 106331 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

TotalSeq™-B0388 anti-mouse CD152

catalog :

106331

quantity :

10 μg

price :

369 USD

clonality :

monoclonal

host :

hamsters

conjugate :

nonconjugated

clone name :

UC10-4B9

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig s7f	Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 5f	Ruscetti M, Morris J, Mezzadra R, Russell J, Leibold J, Romesser P, et al. Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer. Cell. 2020;181:424-441.e21 pubmed publisher
flow cytometry; mouse; loading ...; fig 2c	Canel M, Taggart D, Sims A, Lonergan D, Waizenegger I, Serrels A. T-cell co-stimulation in combination with targeting FAK drives enhanced anti-tumor immunity. elife. 2020;9: pubmed publisher
flow cytometry; mouse; loading ...	Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
	Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
	McDonald Hyman C, Muller J, Loschi M, Thangavelu G, Saha A, Kumari S, et al. The vimentin intermediate filament network restrains regulatory T cell suppression of graft-versus-host disease. J Clin Invest. 2018;128:4604-4621 pubmed publisher
	Klann J, Kim S, Remedios K, He Z, Metz P, Lopez J, et al. Integrin Activation Controls Regulatory T Cell-Mediated Peripheral Tolerance. J Immunol. 2018;200:4012-4023 pubmed publisher
	Marangoni F, Zhang R, Mani V, Thelen M, Ali Akbar N, Warner R, et al. Tumor Tolerance-Promoting Function of Regulatory T Cells Is Optimized by CD28, but Strictly Dependent on Calcineurin. J Immunol. 2018;200:3647-3661 pubmed publisher

product information

Antigen :

CD152

Apps. Abbrev. :

Cat # :

106331

Clone :

UC10-4B9

Item :

TotalSeq™-B0388 anti-mouse CD152

Isotype :

Armenian Hamster IgG

Other Names :

Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

Size :

10 μg

Price (USD) :

369 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

TOTALSEQ_B

Immunogen :

Mouse CTLA-4-mouse IgG2a fusion protein

Application Notes :

The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation^1, in vitro stimulation, in vitro and in vivo blocking^1-4 of ligand binding, and as ELISA capture antibody^5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).;

more info or order :

BioLegend product webpage