PE/Dazzle™ 594 anti-mouse CD152 | BioLegend 106317 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PE/Dazzle™ 594 anti-mouse CD152

catalog :

106317

quantity :

25 μg

price :

138 USD

clonality :

monoclonal

host :

hamsters

conjugate :

clone name :

UC10-4B9

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig s7f	Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Shi B, Geng J, Wang Y, Wei H, Walters B, Li W, et al. Foxp1 Negatively Regulates T Follicular Helper Cell Differentiation and Germinal Center Responses by Controlling Cell Migration and CTLA-4. J Immunol. 2018;200:586-594 pubmed publisher
	Stokes J, Hoffman E, Molina M, Kummet N, Simpson R, Zeng Y, et al. Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia. Oncoimmunology. 2020;9:1758011 pubmed publisher
	Echevarría Vargas I, Reyes Uribe P, Guterres A, Yin X, Kossenkov A, Liu Q, et al. Co-targeting BET and MEK as salvage therapy for MAPK and checkpoint inhibitor-resistant melanoma. EMBO Mol Med. 2018;10: pubmed publisher
	Sugita S, Horie S, Yamada Y, Mochizuki M. Inhibition of B-cell activation by retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2010;51:5783-8 pubmed publisher

product information

Antigen :

CD152

Apps. Abbrev. :

Flow Cytometry

Cat # :

106317

Clone :

UC10-4B9

Item :

PE/Dazzle™ 594 anti-mouse CD152

Isotype :

Armenian Hamster IgG

Other Names :

Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

Size :

25 μg

Price (USD) :

138 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

PE_DZL594

Immunogen :

Mouse CTLA-4-mouse IgG2a fusion protein

Application Notes :

The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro stimulation, in vitro and in vivo blocking 1-4 of ligand binding, and as ELISA capture antibody 5 . To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).

more info or order :

BioLegend product webpage