PerCP/Cyanine5.5 anti-mouse CD152 | BioLegend 106316 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

PerCP/Cyanine5.5 anti-mouse CD152

catalog :

106316

quantity :

100 μg

price :

325 USD

clonality :

monoclonal

host :

hamsters

conjugate :

PerCP/Cy5.5

clone name :

UC10-4B9

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:300; loading ...; fig 6g	Liu Z, Ravindranathan R, Kalinski P, Guo Z, Bartlett D. Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy. Nat Commun. 2017;8:14754 pubmed publisher
	Russell S, Lamprecht D, Mandizvo T, Jones T, Naidoo V, Addicott K, et al. Compromised Metabolic Reprogramming Is an Early Indicator of CD8+ T Cell Dysfunction during Chronic Mycobacterium tuberculosis Infection. Cell Rep. 2019;29:3564-3579.e5 pubmed publisher
	Sugita S, Horie S, Yamada Y, Mochizuki M. Inhibition of B-cell activation by retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2010;51:5783-8 pubmed publisher

product information

Antigen :

CD152

Apps. Abbrev. :

Flow Cytometry

Cat # :

106316

Clone :

UC10-4B9

Item :

PerCP/Cyanine5.5 anti-mouse CD152

Isotype :

Armenian Hamster IgG

Other Names :

Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

Size :

100 μg

Price (USD) :

325 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Armenian Hamster

Conjugate/Tag/Label :

PERCP_CYANINE5.5

Immunogen :

Mouse CTLA-4-mouse IgG2a fusion protein

Application Notes :

The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro stimulation, in vitro and in vivo blocking 1-4 of ligand binding, and as ELISA capture antibody 5 . To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 106327).

more info or order :

BioLegend product webpage