Babco, Signet, Sternberger Monoclonals, Senetek, Covance

antibody

Ultra-LEAF™ Purified anti-mouse CD152

106211

100 μg

110 USD

monoclonal

Syrian golden hamster

nonconjugated

9H10

ELISA, neutralization, flow cytometry

ELISA, FC, Block, Costim

106211

9H10

Ultra-LEAF™ Purified anti-mouse CD152

Syrian Hamster IgG

Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56

100 μg

110 USD

Mouse

Monoclonal

Syrian Hamster

ULEAF

Mouse CTLA-4-human IgG1 fusion protein

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking^1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody^1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No, 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106211 - 106216);.;