product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
TotalSeq™-A0012 anti-mouse CD117 (c-kit)
catalog :
105843
quantity :
10 μg
price :
369 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
2B8
reactivity :
mouse
application :
flow cytometry, mass cytometry
more info or order :
citations: 26
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2b
Seung H, Wröbel J, Wadle C, B xfc hler T, Chiang D, Rettkowski J, et al. P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Res Cardiol. 2022;117:16 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3a
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; fig s1b
Toyama S, Moniaga C, Nakae S, Kurosawa M, Ogawa H, Tominaga M, et al. Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1-1f
Geng G, Liu J, Xu C, Pei Y, Chen L, Mu C, et al. Receptor-mediated mitophagy regulates EPO production and protects against renal anemia. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3e
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Wilson Z, Witt H, Hazlett L, Harman M, Neumann B, Whitman A, et al. Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking. Sci Rep. 2020;10:2142 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7b
Hayes M, Ward S, Crawford G, Seoane R, Jackson W, Kipling D, et al. Inflammation-induced IgE promotes epithelial hyperplasia and tumour growth. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; fig s7
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • mass cytometry; mouse; 1.5 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Paudel S, Baral P, Ghimire L, Bergeron S, Jin L, De Corte J, et al. CXCL1 regulates neutrophil homeostasis in pneumonia-derived sepsis caused by Streptococcus pneumoniae serotype 3. Blood. 2019;: pubmed publisher
  • flow cytometry; mouse; 1-2 ug/ml; fig 2a
Duan S, Koziol White C, Jester W, Nycholat C, Macauley M, Panettieri R, et al. CD33 recruitment inhibits IgE-mediated anaphylaxis and desensitizes mast cells to allergen. J Clin Invest. 2019;129:1387-1401 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Umemoto T, Hashimoto M, Matsumura T, Nakamura Ishizu A, Suda T. Ca2+-mitochondria axis drives cell division in hematopoietic stem cells. J Exp Med. 2018;215:2097-2113 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1c
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Mumau M, Vanderbeck A, Lynch E, Golec S, Emerson S, Punt J. Identification of a Multipotent Progenitor Population in the Spleen That Is Regulated by NR4A1. J Immunol. 2018;200:1078-1087 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Burton O, Tamayo J, Stranks A, Koleoglou K, Oettgen H. Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance. J Allergy Clin Immunol. 2018;141:189-201.e3 pubmed publisher
Al Rifai R, Vandestienne M, Lavillegrand J, Mirault T, Cornebise J, Poisson J, et al. JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm. Nat Commun. 2022;13:6592 pubmed publisher
Guilliams M, Bonnardel J, Haest B, Vanderborght B, Wagner C, Remmerie A, et al. Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches. Cell. 2022;185:379-396.e38 pubmed publisher
Rødahl I, Gotley J, Andersen S, Yu M, Mehdi A, Christ A, et al. Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq. STAR Protoc. 2021;2:100842 pubmed publisher
Guldner I, Golomb S, Wang Q, Wang E, Zhang S. Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes. STAR Protoc. 2021;2:100537 pubmed publisher
Golomb S, Guldner I, Zhao A, Wang Q, Palakurthi B, Aleksandrovic E, et al. Multi-modal Single-Cell Analysis Reveals Brain Immune Landscape Plasticity during Aging and Gut Microbiota Dysbiosis. Cell Rep. 2020;33:108438 pubmed publisher
Guldner I, Wang Q, Yang L, Golomb S, Zhao Z, Lopez J, et al. CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10. Cell. 2020;183:1234-1248.e25 pubmed publisher
Lu Y, Liao J, Su S. Protocol for Single-Cell Analysis of Tumor-Infiltrating B Cells Isolated from Human Breast Cancer Tissue Before and After Neo-adjuvant Chemotherapy. STAR Protoc. 2020;1:100040 pubmed publisher
Lu Y, Zhao Q, Liao J, Song E, Xia Q, Pan J, et al. Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity. Cell. 2020;180:1081-1097.e24 pubmed publisher
product information
Antigen :
CD117
Apps. Abbrev. :
PG
Cat # :
105843
Clone :
2B8
Item :
TotalSeq™-A0012 anti-mouse CD117 (c-kit)
Isotype :
Rat IgG2b, κ
Other Names :
c-KIT, Stem Cell Factor Receptor (SCFR)
Size :
10 μg
Price (USD) :
369 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
TOTALSEQ_A
Immunogen :
Mouse bone marrow mast cells
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation^1, immunohistochemistry of acetone fixed frozen sections^2, and spatial biology (IBEX)^5,6. The 2B8 antibody does not block c-Kit activity.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA