product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD117 (c-kit)
catalog :
105817
quantity :
25 μg
price :
101 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
2B8
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section, mass cytometry
more info or order :
citations: 36
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2b
Seung H, Wröbel J, Wadle C, B xfc hler T, Chiang D, Rettkowski J, et al. P2Y12-dependent activation of hematopoietic stem and progenitor cells promotes emergency hematopoiesis after myocardial infarction. Basic Res Cardiol. 2022;117:16 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 3a
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; fig s1b
Toyama S, Moniaga C, Nakae S, Kurosawa M, Ogawa H, Tominaga M, et al. Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1-1f
Geng G, Liu J, Xu C, Pei Y, Chen L, Mu C, et al. Receptor-mediated mitophagy regulates EPO production and protects against renal anemia. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3e
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Wilson Z, Witt H, Hazlett L, Harman M, Neumann B, Whitman A, et al. Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking. Sci Rep. 2020;10:2142 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1b
Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7b
Hayes M, Ward S, Crawford G, Seoane R, Jackson W, Kipling D, et al. Inflammation-induced IgE promotes epithelial hyperplasia and tumour growth. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; fig s7
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • mass cytometry; mouse; 1.5 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Qian L, Bajana S, Georgescu C, Peng V, Wang H, Adrianto I, et al. Suppression of ILC2 differentiation from committed T cell precursors by E protein transcription factors. J Exp Med. 2019;216:884-899 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Paudel S, Baral P, Ghimire L, Bergeron S, Jin L, De Corte J, et al. CXCL1 regulates neutrophil homeostasis in pneumonia-derived sepsis caused by Streptococcus pneumoniae serotype 3. Blood. 2019;: pubmed publisher
  • flow cytometry; mouse; 1-2 ug/ml; fig 2a
Duan S, Koziol White C, Jester W, Nycholat C, Macauley M, Panettieri R, et al. CD33 recruitment inhibits IgE-mediated anaphylaxis and desensitizes mast cells to allergen. J Clin Invest. 2019;129:1387-1401 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1
Choi H, Suwanpradid J, Kim I, Staats H, Haniffa M, Macleod A, et al. Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles. Science. 2018;362: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Umemoto T, Hashimoto M, Matsumura T, Nakamura Ishizu A, Suda T. Ca2+-mitochondria axis drives cell division in hematopoietic stem cells. J Exp Med. 2018;215:2097-2113 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Baba O, Horie T, Nakao T, Hakuno D, Nakashima Y, Nishi H, et al. MicroRNA 33 Regulates the Population of Peripheral Inflammatory Ly6Chigh Monocytes through Dual Pathways. Mol Cell Biol. 2018;38: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1c
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Mumau M, Vanderbeck A, Lynch E, Golec S, Emerson S, Punt J. Identification of a Multipotent Progenitor Population in the Spleen That Is Regulated by NR4A1. J Immunol. 2018;200:1078-1087 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Singh P, Hoggatt J, Kamocka M, Mohammad K, Saunders M, Li H, et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest. 2017;127:4527-4540 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Ando T, Kashiwakura J, Itoh Nagato N, Yamashita H, Baba M, Kawakami Y, et al. Histamine-releasing factor enhances food allergy. J Clin Invest. 2017;127:4541-4553 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Burton O, Tamayo J, Stranks A, Koleoglou K, Oettgen H. Allergen-specific IgG antibody signaling through FcγRIIb promotes food tolerance. J Allergy Clin Immunol. 2018;141:189-201.e3 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Damle S, Martin R, Cross J, Conrad D. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis. Mucosal Immunol. 2017;10:205-214 pubmed publisher
  • flow cytometry; mouse
Shade K, Platzer B, Washburn N, Mani V, Bartsch Y, Conroy M, et al. A single glycan on IgE is indispensable for initiation of anaphylaxis. J Exp Med. 2015;212:457-67 pubmed publisher
  • flow cytometry; mouse; tbl 1
Magri G, Miyajima M, Bascones S, Mortha A, Puga I, Cassis L, et al. Innate lymphoid cells integrate stromal and immunological signals to enhance antibody production by splenic marginal zone B cells. Nat Immunol. 2014;15:354-364 pubmed publisher
Barkaway A, Rolas L, Joulia R, Bodkin J, Lenn T, Owen Woods C, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity. 2021;54:1494-1510.e7 pubmed publisher
Riedel S, Lu C, Xie H, Nestler K, Vermunt M, Lenard A, et al. Intrinsically disordered Meningioma-1 stabilizes the BAF complex to cause AML. Mol Cell. 2021;81:2332-2348.e9 pubmed publisher
Pietronigro E, Zenaro E, Bianca V, Dusi S, Terrabuio E, Iannoto G, et al. Blockade of α4 integrins reduces leukocyte-endothelial interactions in cerebral vessels and improves memory in a mouse model of Alzheimer's disease. Sci Rep. 2019;9:12055 pubmed publisher
Barman P, Pang J, Urao N, Koh T. Skin Wounding-Induced Monocyte Expansion in Mice Is Not Abrogated by IL-1 Receptor 1 Deficiency. J Immunol. 2019;202:2720-2727 pubmed publisher
Trompette A, Gollwitzer E, Pattaroni C, Lopez Mejia I, Riva E, Pernot J, et al. Dietary Fiber Confers Protection against Flu by Shaping Ly6c- Patrolling Monocyte Hematopoiesis and CD8+ T Cell Metabolism. Immunity. 2018;48:992-1005.e8 pubmed publisher
Hoshii T, Cifani P, Feng Z, Huang C, Koche R, Chen C, et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell. 2018;172:1007-1021.e17 pubmed publisher
Amouzegar A, Mittal S, Sahu A, Sahu S, Chauhan S. Mesenchymal Stem Cells Modulate Differentiation of Myeloid Progenitor Cells During Inflammation. Stem Cells. 2017;35:1532-1541 pubmed publisher
Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Harhouri K, Kebir A, Guillet B, Foucault Bertaud A, Voytenko S, Piercecchi Marti M, et al. Soluble CD146 displays angiogenic properties and promotes neovascularization in experimental hind-limb ischemia. Blood. 2010;115:3843-51 pubmed publisher
Bachelet I, Munitz A, Berent Maoz B, Mankuta D, Levi Schaffer F. Suppression of normal and malignant kit signaling by a bispecific antibody linking kit with CD300a. J Immunol. 2008;180:6064-9 pubmed
Podd B, Thoits J, Whitley N, Cheng H, Kudla K, Taniguchi H, et al. T cells in cryptopatch aggregates share TCR gamma variable region junctional sequences with gamma delta T cells in the small intestinal epithelium of mice. J Immunol. 2006;176:6532-42 pubmed
product information
Antigen :
CD117
Apps. Abbrev. :
FC, IHC-F
Cat # :
105817
Clone :
2B8
Item :
Alexa Fluor® 647 anti-mouse CD117 (c-kit)
Isotype :
Rat IgG2b, κ
Other Names :
c-KIT, Stem Cell Factor Receptor (SCFR)
Size :
25 μg
Price (USD) :
101 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
Mouse bone marrow mast cells
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation^1, immunohistochemistry of acetone fixed frozen sections^2, and spatial biology (IBEX)^5,6. The 2B8 antibody does not block c-Kit activity.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA