product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse CD86
catalog :
105053
quantity :
5 mg
price :
539 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
GL-1
reactivity :
mouse
application :
flow cytometry, mass cytometry
more info or order :
citations: 23
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100
Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Kawakubo A, Miyagi M, Yokozeki Y, Nakawaki M, Takano S, Satoh M, et al. Origin of M2 Mϕ and its macrophage polarization by TGF-β in a mice intervertebral injury model. Int J Immunopathol Pharmacol. 2022;36:3946320221103792 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 2f, s2l
Bartsch P, Kilian C, Hellmig M, Paust H, Borchers A, Sivayoganathan A, et al. Th17 cell plasticity towards a T-bet-dependent Th1 phenotype is required for bacterial control in Staphylococcus aureus infection. PLoS Pathog. 2022;18:e1010430 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 8a
Satofuka H, Abe S, Moriwaki T, Okada A, Kazuki K, Tanaka H, et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Nat Commun. 2022;13:1841 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7e
Quách T, Huang W, Sahu R, Diadhiou C, Raparia C, Johnson R, et al. Context-dependent induction of autoimmunity by TNF signaling deficiency. JCI Insight. 2022;7: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s6c
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2a
Nordlohne J, Hulsmann I, Schwafertz S, Zgrajek J, Grundmann M, von Vietinghoff S, et al. A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes. Sci Rep. 2021;11:13251 pubmed publisher
  • flow cytometry; mouse; fig 6c
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5g
Chen J, Cao X, Li B, Zhao Z, Chen S, Lai S, et al. Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Front Immunol. 2020;11:621757 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s2-2a
Xu A, Barbosa R, Calado D. Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6
Giri K, De Beaurepaire L, Jegou D, Lavy M, Mosser M, Dupont A, et al. Molecular and Functional Diversity of Distinct Subpopulations of the Stressed Insulin-Secreting Cell's Vesiculome. Front Immunol. 2020;11:1814 pubmed publisher
  • flow cytometry; mouse; fig 2d
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse
Zhao L, Hu S, Davila M, Yang J, Lin Y, Albanese J, et al. Coordinated co-migration of CCR10+ antibody-producing B cells with helper T cells for colonic homeostatic regulation. Mucosal Immunol. 2021;14:420-430 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Cignarella F, Filipello F, Bollman B, Cantoni C, Locca A, Mikesell R, et al. TREM2 activation on microglia promotes myelin debris clearance and remyelination in a model of multiple sclerosis. Acta Neuropathol. 2020;140:513-534 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f, s2b
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Takai A, Kikuchi K, Ichimura M, Tsuneyama K, Moritoki Y, Matsumoto K, et al. Fructo-oligosaccharides ameliorate steatohepatitis, visceral adiposity, and associated chronic inflammation via increased production of short-chain fatty acids in a mouse model of non-alcoholic steatohepatitis. BMC Gastroenterol. 2020;20:46 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • mass cytometry; mouse; 0.75 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4g
Jia S, Li W, Liu P, Xu L. A role of eosinophils in mediating the anti-tumour effect of cryo-thermal treatment. Sci Rep. 2019;9:13214 pubmed publisher
Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
product information
Antigen :
CD86
Apps. Abbrev. :
FC
Cat # :
105053
Clone :
GL-1
Item :
Ultra-LEAF™ Purified anti-mouse CD86
Isotype :
Rat IgG2a, κ
Other Names :
B7-2, B70, Ly-58
Size :
5 mg
Price (USD) :
539 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
ULEAF
Immunogen :
LPS-activated CBA/Ca mouse splenic B cells
Application Notes :
The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation^1, immunohistochemical staining of acetone-fixed frozen sections^2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses^1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA