product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
TotalSeq™-A0200 anti-mouse CD86
catalog :
105047
quantity :
10 μg
price :
369 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
GL-1
reactivity :
mouse
application :
flow cytometry, mass cytometry
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100
Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Kawakubo A, Miyagi M, Yokozeki Y, Nakawaki M, Takano S, Satoh M, et al. Origin of M2 Mϕ and its macrophage polarization by TGF-β in a mice intervertebral injury model. Int J Immunopathol Pharmacol. 2022;36:3946320221103792 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 2f, s2l
Bartsch P, Kilian C, Hellmig M, Paust H, Borchers A, Sivayoganathan A, et al. Th17 cell plasticity towards a T-bet-dependent Th1 phenotype is required for bacterial control in Staphylococcus aureus infection. PLoS Pathog. 2022;18:e1010430 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 8a
Satofuka H, Abe S, Moriwaki T, Okada A, Kazuki K, Tanaka H, et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Nat Commun. 2022;13:1841 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7e
Quách T, Huang W, Sahu R, Diadhiou C, Raparia C, Johnson R, et al. Context-dependent induction of autoimmunity by TNF signaling deficiency. JCI Insight. 2022;7: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s6c
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2a
Nordlohne J, Hulsmann I, Schwafertz S, Zgrajek J, Grundmann M, von Vietinghoff S, et al. A flow cytometry approach reveals heterogeneity in conventional subsets of murine renal mononuclear phagocytes. Sci Rep. 2021;11:13251 pubmed publisher
  • flow cytometry; mouse; fig 6c
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5g
Chen J, Cao X, Li B, Zhao Z, Chen S, Lai S, et al. Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Front Immunol. 2020;11:621757 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s2-2a
Xu A, Barbosa R, Calado D. Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6
Giri K, De Beaurepaire L, Jegou D, Lavy M, Mosser M, Dupont A, et al. Molecular and Functional Diversity of Distinct Subpopulations of the Stressed Insulin-Secreting Cell's Vesiculome. Front Immunol. 2020;11:1814 pubmed publisher
  • flow cytometry; mouse; fig 2d
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse
Zhao L, Hu S, Davila M, Yang J, Lin Y, Albanese J, et al. Coordinated co-migration of CCR10+ antibody-producing B cells with helper T cells for colonic homeostatic regulation. Mucosal Immunol. 2021;14:420-430 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Cignarella F, Filipello F, Bollman B, Cantoni C, Locca A, Mikesell R, et al. TREM2 activation on microglia promotes myelin debris clearance and remyelination in a model of multiple sclerosis. Acta Neuropathol. 2020;140:513-534 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f, s2b
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Takai A, Kikuchi K, Ichimura M, Tsuneyama K, Moritoki Y, Matsumoto K, et al. Fructo-oligosaccharides ameliorate steatohepatitis, visceral adiposity, and associated chronic inflammation via increased production of short-chain fatty acids in a mouse model of non-alcoholic steatohepatitis. BMC Gastroenterol. 2020;20:46 pubmed publisher
  • flow cytometry; mouse; 1 ug/ml; loading ...; fig 3e
Wang J, Li P, Yu Y, Fu Y, Jiang H, Lu M, et al. Pulmonary surfactant-biomimetic nanoparticles potentiate heterosubtypic influenza immunity. Science. 2020;367: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4b
Reinhard K, Rengstl B, Oehm P, Michel K, Billmeier A, Hayduk N, et al. An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors. Science. 2020;367:446-453 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4
Eastman A, Xu J, Bermik J, Potchen N, den Dekker A, Neal L, et al. Epigenetic stabilization of DC and DC precursor classical activation by TNFα contributes to protective T cell polarization. Sci Adv. 2019;5:eaaw9051 pubmed publisher
  • mass cytometry; mouse; 0.75 ug/ml; loading ...; fig 5d
Guo C, Allen B, Hiam K, Dodd D, Van Treuren W, Higginbottom S, et al. Depletion of microbiome-derived molecules in the host using Clostridium genetics. Science. 2019;366: pubmed publisher
  • mass cytometry; mouse; loading ...; fig 1a, 1c, s1
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4g
Jia S, Li W, Liu P, Xu L. A role of eosinophils in mediating the anti-tumour effect of cryo-thermal treatment. Sci Rep. 2019;9:13214 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s13a
Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1c
McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig s9a, s9b
Benmerzoug S, Rose S, Bounab B, Gosset D, Duneau L, Chenuet P, et al. STING-dependent sensing of self-DNA drives silica-induced lung inflammation. Nat Commun. 2018;9:5226 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 4h
Sang A, Danhorn T, Peterson J, Rankin A, O Connor B, Leach S, et al. Innate and adaptive signals enhance differentiation and expansion of dual-antibody autoreactive B cells in lupus. Nat Commun. 2018;9:3973 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4b
Wilson K, Liu H, Healey G, Vuong V, Ishido S, Herold M, et al. MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway. PLoS ONE. 2018;13:e0200540 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s6a
Macdougall C, Wood E, Loschko J, Scagliotti V, Cassidy F, Robinson M, et al. Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets. Cell Metab. 2018;27:588-601.e4 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1b
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Turner D, Goldklang M, Cvetkovski F, Paik D, Trischler J, Barahona J, et al. Biased Generation and In Situ Activation of Lung Tissue-Resident Memory CD4 T Cells in the Pathogenesis of Allergic Asthma. J Immunol. 2018;200:1561-1569 pubmed publisher
Al Rifai R, Vandestienne M, Lavillegrand J, Mirault T, Cornebise J, Poisson J, et al. JAK2V617F mutation drives vascular resident macrophages toward a pathogenic phenotype and promotes dissecting aortic aneurysm. Nat Commun. 2022;13:6592 pubmed publisher
Pisu D, Huang L, Narang V, Theriault M, L xea Bury G, Lee B, et al. Single cell analysis of M. tuberculosis phenotype and macrophage lineages in the infected lung. J Exp Med. 2021;218: pubmed publisher
Guldner I, Golomb S, Wang Q, Wang E, Zhang S. Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes. STAR Protoc. 2021;2:100537 pubmed publisher
Golomb S, Guldner I, Zhao A, Wang Q, Palakurthi B, Aleksandrovic E, et al. Multi-modal Single-Cell Analysis Reveals Brain Immune Landscape Plasticity during Aging and Gut Microbiota Dysbiosis. Cell Rep. 2020;33:108438 pubmed publisher
Guldner I, Wang Q, Yang L, Golomb S, Zhao Z, Lopez J, et al. CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10. Cell. 2020;183:1234-1248.e25 pubmed publisher
Wirka R, Wagh D, Paik D, Pjanic M, Nguyen T, Miller C, et al. Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis. Nat Med. 2019;25:1280-1289 pubmed publisher
product information
Antigen :
CD86
Apps. Abbrev. :
PG
Cat # :
105047
Clone :
GL-1
Item :
TotalSeq™-A0200 anti-mouse CD86
Isotype :
Rat IgG2a, κ
Other Names :
B7-2, B70, Ly-58
Size :
10 μg
Price (USD) :
369 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
TOTALSEQ_A
Immunogen :
LPS-activated CBA/Ca mouse splenic B cells
Application Notes :
The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation^1, immunohistochemical staining of acetone-fixed frozen sections^2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses^1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA