product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse CD86
catalog :
105012
quantity :
100 μg
price :
264 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
GL-1
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 53
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; loading ...; fig s6c
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a, s3b, s3c, s4a
Cohen G, Chandran P, Lorsung R, Tomlinson L, Sundby M, Burks S, et al. The Impact of Focused Ultrasound in Two Tumor Models: Temporal Alterations in the Natural History on Tumor Microenvironment and Immune Cell Response. Cancers (Basel). 2020;12: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3d
Yan D, Wang J, Sun H, Zamani A, Zhang H, Chen W, et al. TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. J Exp Med. 2020;217: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s13a
Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...
Gabunia K, Ellison S, Kelemen S, Kako F, Cornwell W, Rogers T, et al. IL-19 Halts Progression of Atherosclerotic Plaque, Polarizes, and Increases Cholesterol Uptake and Efflux in Macrophages. Am J Pathol. 2016;186:1361-74 pubmed publisher
  • flow cytometry; mouse
Kawamoto S, Tran T, Maruya M, Suzuki K, Doi Y, Tsutsui Y, et al. The inhibitory receptor PD-1 regulates IgA selection and bacterial composition in the gut. Science. 2012;336:485-9 pubmed publisher
Anderson A, Wu I, Parrillo A, Wolf M, Maestas D, Graham I, et al. An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial. NPJ Regen Med. 2022;7:6 pubmed publisher
Wu L, Zhan Q, Liu P, Zheng H, Liu M, Min J, et al. LncRNA TCONS_00145741 Knockdown Prevents Thrombin-Induced M1 Differentiation of Microglia in Intracerebral Hemorrhage by Enhancing the Interaction Between DUSP6 and JNK. Front Cell Dev Biol. 2021;9:684842 pubmed publisher
Zheng Y, Fang D, Huang C, Zhao L, Gan L, Chen Y, et al. Gentiana scabra Restrains Hepatic Pro-Inflammatory Macrophages to Ameliorate Non-Alcoholic Fatty Liver Disease. Front Pharmacol. 2021;12:816032 pubmed publisher
Zhang W, Wang Y, Li C, Xu Y, Wang X, Wu D, et al. Extracellular CIRP-Impaired Rab26 Restrains EPOR-Mediated Macrophage Polarization in Acute Lung Injury. Front Immunol. 2021;12:768435 pubmed publisher
Qiu N, Yang X, Zhang Y, Zhang J, Ji J, Zhang Y, et al. A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases. J Nanobiotechnology. 2021;19:428 pubmed publisher
Sun S, Xu X, Liang L, Wang X, Bai X, Zhu L, et al. Lactic Acid-Producing Probiotic Saccharomyces cerevisiae Attenuates Ulcerative Colitis via Suppressing Macrophage Pyroptosis and Modulating Gut Microbiota. Front Immunol. 2021;12:777665 pubmed publisher
Qi J, Jin F, You Y, Du Y, Liu D, Xu X, et al. Synergistic effect of tumor chemo-immunotherapy induced by leukocyte-hitchhiking thermal-sensitive micelles. Nat Commun. 2021;12:4755 pubmed publisher
Dai X, Bu X, Gao Y, Guo J, Hu J, Jiang C, et al. Energy status dictates PD-L1 protein abundance and anti-tumor immunity to enable checkpoint blockade. Mol Cell. 2021;81:2317-2331.e6 pubmed publisher
Wilhelm J, Quiñones Pérez M, Wang J, Wang X, Basava V, Gao J. Antigen folding improves loading efficiency and antitumor efficacy of PC7A nanoparticle vaccine. J Control Release. 2021;329:353-360 pubmed publisher
Huang L, Fang X, Zhang X, Wu W, Yao H, Fang Q. RON Expression Mediates Lipopolysaccharide-Mediated Dendritic Cell Maturation via March-I. Front Cell Infect Microbiol. 2020;10:606340 pubmed publisher
Trivedi S, Labuz D, Anderson C, Araújo C, Blair A, Middleton E, et al. Mucosal-associated invariant T (MAIT) cells mediate protective host responses in sepsis. elife. 2020;9: pubmed publisher
Gong N, Zhang Y, Teng X, Wang Y, Huo S, Qing G, et al. Proton-driven transformable nanovaccine for cancer immunotherapy. Nat Nanotechnol. 2020;15:1053-1064 pubmed publisher
Lu S, Pan H, Hsu Y, Chang K, Wu L, Chen W, et al. IL-20 antagonist suppresses PD-L1 expression and prolongs survival in pancreatic cancer models. Nat Commun. 2020;11:4611 pubmed publisher
Liang J, Wang H, Ding W, Huang J, Zhou X, Wang H, et al. Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity. Sci Adv. 2020;6:eabc3646 pubmed publisher
Coronel M, MARTIN K, Hunckler M, Barber G, O Neill E, Medina J, et al. Immunotherapy via PD-L1-presenting biomaterials leads to long-term islet graft survival. Sci Adv. 2020;6:eaba5573 pubmed publisher
Ukidve A, Zhao Z, Fehnel A, Krishnan V, Pan D, Gao Y, et al. Erythrocyte-driven immunization via biomimicry of their natural antigen-presenting function. Proc Natl Acad Sci U S A. 2020;117:17727-17736 pubmed publisher
Yu X, Chan H, Fisher H, Penfold C, Kim J, Inzhelevskaya T, et al. Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity. Cancer Cell. 2020;37:850-866.e7 pubmed publisher
Bi C, Li X, Qu H, Sun L, An Y, Hong Y, et al. Calcitriol inhibits osteoclastogenesis in an inflammatory environment by changing the proportion and function of T helper cell subsets (Th2/Th17). Cell Prolif. 2020;53:e12827 pubmed publisher
Sitnik S, Masemann D, Leite Dantas R, Wixler V, Ludwig S. PD-1 IC Inhibition Synergistically Improves Influenza A Virus-Mediated Oncolysis of Metastatic Pulmonary Melanoma. Mol Ther Oncolytics. 2020;17:190-204 pubmed publisher
He J, Han R, Yu G, Lavin M, Jia Q, Cui P, et al. Epimedium Polysaccharide Ameliorates Benzene-Induced Aplastic Anemia in Mice. Evid Based Complement Alternat Med. 2020;2020:5637507 pubmed publisher
Mul Fedele M, Aiello I, Caldart C, Golombek D, Marpegan L, Paladino N. Differential Thermoregulatory and Inflammatory Patterns in the Circadian Response to LPS-Induced Septic Shock. Front Cell Infect Microbiol. 2020;10:100 pubmed publisher
Yamada K, Heim C, Xi X, Attri K, Wang D, Zhang W, et al. Monocyte metabolic reprogramming promotes pro-inflammatory activity and Staphylococcus aureus biofilm clearance. PLoS Pathog. 2020;16:e1008354 pubmed publisher
Yu X, Dai Y, Zhao Y, Qi S, Liu L, Lu L, et al. Melittin-lipid nanoparticles target to lymph nodes and elicit a systemic anti-tumor immune response. Nat Commun. 2020;11:1110 pubmed publisher
Qi S, Lu L, Zhou F, Chen Y, Xu M, Chen L, et al. Neutrophil infiltration and whole-cell vaccine elicited by N-dihydrogalactochitosan combined with NIR phototherapy to enhance antitumor immune response and T cell immune memory. Theranostics. 2020;10:1814-1832 pubmed publisher
Tsuchiya N, Zhang R, Iwama T, Ueda N, Liu T, Tatsumi M, et al. Type I Interferon Delivery by iPSC-Derived Myeloid Cells Elicits Antitumor Immunity via XCR1+ Dendritic Cells. Cell Rep. 2019;29:162-175.e9 pubmed publisher
Kashyap A, Fernandez Rodriguez L, Zhao Y, Monaco G, Trefny M, Yoshida N, et al. GEF-H1 Signaling upon Microtubule Destabilization Is Required for Dendritic Cell Activation and Specific Anti-tumor Responses. Cell Rep. 2019;28:3367-3380.e8 pubmed publisher
Yang C, He B, Zheng Q, Wang D, Qin M, Zhang H, et al. Nano-encapsulated tryptanthrin derivative for combined anticancer therapy via inhibiting indoleamine 2,3-dioxygenase and inducing immunogenic cell death. Nanomedicine (Lond). 2019;14:2423-2440 pubmed publisher
Luck H, Khan S, Kim J, Copeland J, Revelo X, Tsai S, et al. Gut-associated IgA+ immune cells regulate obesity-related insulin resistance. Nat Commun. 2019;10:3650 pubmed publisher
Finkin S, Hartweger H, Oliveira T, Kara E, Nussenzweig M. Protein Amounts of the MYC Transcription Factor Determine Germinal Center B Cell Division Capacity. Immunity. 2019;51:324-336.e5 pubmed publisher
Liu W, Zou M, Liu T, Zeng J, Li X, Yu W, et al. Cytomembrane nanovaccines show therapeutic effects by mimicking tumor cells and antigen presenting cells. Nat Commun. 2019;10:3199 pubmed publisher
Radulović V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, et al. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis. Cell Rep. 2019;27:2826-2836.e5 pubmed publisher
Xiao Y, Muhuri M, Li S, Qin W, Xu G, Luo L, et al. Circumventing cellular immunity by miR142-mediated regulation sufficiently supports rAAV-delivered OVA expression without activating humoral immunity. JCI Insight. 2019;5: pubmed publisher
Arora H, Wilcox S, Johnson L, Munro L, Eyford B, Pfeifer C, et al. The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock. Immunity. 2019;50:418-431.e6 pubmed publisher
Yu X, Chen L, Liu J, Dai B, Xu G, Shen G, et al. Immune modulation of liver sinusoidal endothelial cells by melittin nanoparticles suppresses liver metastasis. Nat Commun. 2019;10:574 pubmed publisher
Edgar L, Kawasaki N, Nycholat C, Paulson J. Targeted Delivery of Antigen to Activated CD169+ Macrophages Induces Bias for Expansion of CD8+ T Cells. Cell Chem Biol. 2019;26:131-136.e4 pubmed publisher
Shan M, Carrillo J, Yeste A, Gutzeit C, Segura Garzón D, Walland A, et al. Secreted IgD Amplifies Humoral T Helper 2 Cell Responses by Binding Basophils via Galectin-9 and CD44. Immunity. 2018;49:709-724.e8 pubmed publisher
Hou Y, Liang H, Rao E, Zheng W, Huang X, Deng L, et al. Non-canonical NF-κB Antagonizes STING Sensor-Mediated DNA Sensing in Radiotherapy. Immunity. 2018;49:490-503.e4 pubmed publisher
Bhattacharjee P, Keyel P. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018;8:6458 pubmed publisher
Zhao F, Xiao C, Evans K, Theivanthiran T, DeVito N, Holtzhausen A, et al. Paracrine Wnt5a-β-Catenin Signaling Triggers a Metabolic Program that Drives Dendritic Cell Tolerization. Immunity. 2018;48:147-160.e7 pubmed publisher
Sellau J, Alvarado C, Hoenow S, Mackroth M, Kleinschmidt D, Huber S, et al. IL-22 dampens the T cell response in experimental malaria. Sci Rep. 2016;6:28058 pubmed publisher
Singhal P, Sassi S, Lan L, Au P, Halvorsen S, Fukumura D, et al. Mouse embryonic fibroblasts exhibit extensive developmental and phenotypic diversity. Proc Natl Acad Sci U S A. 2016;113:122-7 pubmed publisher
Seki T, Yamamoto M, Taguchi Y, Miyauchi M, Akiyama N, Yamaguchi N, et al. Visualization of RelB expression and activation at the single-cell level during dendritic cell maturation in Relb-Venus knock-in mice. J Biochem. 2015;158:485-95 pubmed publisher
de Verteuil D, Rouette A, Hardy M, Lavallée S, Trofimov A, Gaucher E, et al. Immunoproteasomes shape the transcriptome and regulate the function of dendritic cells. J Immunol. 2014;193:1121-32 pubmed publisher
Benson J, Shepherd D. Aryl hydrocarbon receptor activation by TCDD reduces inflammation associated with Crohn's disease. Toxicol Sci. 2011;120:68-78 pubmed publisher
Klinger M, Dattilio A, Vizzard M. Expression of cyclooxygenase-2 in urinary bladder in rats with cyclophosphamide-induced cystitis. Am J Physiol Regul Integr Comp Physiol. 2007;293:R677-85 pubmed
product information
Antigen :
CD86
Apps. Abbrev. :
Flow Cytometry
Cat # :
105012
Clone :
GL-1
Item :
APC anti-mouse CD86
Isotype :
Rat IgG2a, κ
Other Names :
B7-2, B70, Ly-58
Size :
100 μg
Price (USD) :
264 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
LPS-activated CBA/Ca mouse splenic B cells
Application Notes :
The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemical staining of acetone-fixed frozen sections 2,6 , immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses 1-6 . GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA