product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD86
catalog :
105007
quantity :
50 μg
price :
110 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
GL-1
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 63
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s7-1a
Tran N, Ferreira L, Alvarez Moya B, Buttiglione V, Ferrini B, Zordan P, et al. Continuous sensing of IFNα by hepatic endothelial cells shapes a vascular antimetastatic barrier. elife. 2022;11: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 4b, s7c
Zhang Y, Huo F, Cao Q, Jia R, Huang Q, Wang Z, et al. FimH confers mannose-targeting ability to Bacillus Calmette-Guerin for improved immunotherapy in bladder cancer. J Immunother Cancer. 2022;10: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2c, e2i, e4c, e4j
Zhou Q, Liang J, Yang T, Liu J, Li B, Li Y, et al. Carfilzomib modulates tumor microenvironment to potentiate immune checkpoint therapy for cancer. EMBO Mol Med. 2022;14:e14502 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; human; loading ...
Zhou J, Pei X, Yang Y, Wang Z, Gao W, Ye R, et al. Orphan nuclear receptor TLX promotes immunosuppression via its transcriptional activation of PD-L1 in glioma. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4f
Sewastianik T, Straubhaar J, Zhao J, Samur M, Adler K, Tanton H, et al. miR-15a/16-1 deletion in activated B cells promotes plasma cell and mature B-cell neoplasms. Blood. 2021;137:1905-1919 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4g
Jia S, Li W, Liu P, Xu L. A role of eosinophils in mediating the anti-tumour effect of cryo-thermal treatment. Sci Rep. 2019;9:13214 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s4h
Knox T, Sahakian E, Banik D, Hadley M, Palmer E, Noonepalle S, et al. Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells. Sci Rep. 2019;9:6136 pubmed publisher
  • flow cytometry; mouse; 1:150; loading ...; fig s6a
García Nores G, Ly C, Cuzzone D, Kataru R, Hespe G, Torrisi J, et al. CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema. Nat Commun. 2018;9:1970 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1b
Yeh C, Nojima T, Kuraoka M, Kelsoe G. Germinal center entry not selection of B cells is controlled by peptide-MHCII complex density. Nat Commun. 2018;9:928 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Schweighoffer E, Nys J, Vanes L, Smithers N, Tybulewicz V. TLR4 signals in B lymphocytes are transduced via the B cell antigen receptor and SYK. J Exp Med. 2017;214:1269-1280 pubmed publisher
  • flow cytometry; mouse; fig 8
Liechtenstein T, Perez Janices N, Blanco Luquin I, Goyvaerts C, Schwarze J, Dufait I, et al. Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy. Oncoimmunology. 2014;3:e945378 pubmed
  • flow cytometry; mouse
White C, Villarino N, Sloan S, Ganusov V, Schmidt N. Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol. 2015;194:697-708 pubmed publisher
  • flow cytometry; mouse
Škrnjug I, Guzmán C, Rueckert C, Ruecker C. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS ONE. 2014;9:e110150 pubmed publisher
  • flow cytometry; mouse
Kawamoto S, Tran T, Maruya M, Suzuki K, Doi Y, Tsutsui Y, et al. The inhibitory receptor PD-1 regulates IgA selection and bacterial composition in the gut. Science. 2012;336:485-9 pubmed publisher
Jiang H, Zhou L, Shen N, Ning X, Wu D, Jiang K, et al. M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway. Cell Death Dis. 2022;13:183 pubmed publisher
Wu Q, Sun S, Wei L, Liu M, Liu H, Liu T, et al. Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3. Cell Mol Life Sci. 2022;79:137 pubmed publisher
El Hindi K, Brachtendorf S, Hartel J, Oertel S, Birod K, Merz N, et al. T-Cell-Specific CerS4 Depletion Prolonged Inflammation and Enhanced Tumor Burden in the AOM/DSS-Induced CAC Model. Int J Mol Sci. 2022;23: pubmed publisher
Jiang Q, Qiao B, Lin X, Cao J, Zhang N, Guo H, et al. A hydrogen peroxide economizer for on-demand oxygen production-assisted robust sonodynamic immunotherapy. Theranostics. 2022;12:59-75 pubmed publisher
Shiozawa S, Tsumiyama K, Miyazaki Y, Uto K, Sakurai K, Nakashima T, et al. DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus. iScience. 2022;25:103537 pubmed publisher
Liu X, Ye N, Liu S, Guan J, Deng Q, Zhang Z, et al. Hyperbaric Oxygen Boosts PD-1 Antibody Delivery and T Cell Infiltration for Augmented Immune Responses Against Solid Tumors. Adv Sci (Weinh). 2021;8:e2100233 pubmed publisher
Shi G, Hu M, Chen C, Fu J, Shao S, Zhou Y, et al. Methotrexate enhances antigen presentation and maturation of tumour antigen-loaded dendritic cells through NLRP3 inflammasome activation: a strategy for dendritic cell-based cancer vaccine. Ther Adv Med Oncol. 2021;13:1758835920987056 pubmed publisher
Jiang Y, Huang J, Xu C, Pu K. Activatable polymer nanoagonist for second near-infrared photothermal immunotherapy of cancer. Nat Commun. 2021;12:742 pubmed publisher
Xu W, Snell L, Guo M, Boukhaled G, Macleod B, Li M, et al. Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection. Immunity. 2021;54:526-541.e7 pubmed publisher
Huang Z, Wang Y, Yao D, Wu J, Hu Y, Yuan A. Nanoscale coordination polymers induce immunogenic cell death by amplifying radiation therapy mediated oxidative stress. Nat Commun. 2021;12:145 pubmed publisher
Yin X, Zeng W, Wu B, Wang L, Wang Z, Tian H, et al. PPARα Inhibition Overcomes Tumor-Derived Exosomal Lipid-Induced Dendritic Cell Dysfunction. Cell Rep. 2020;33:108278 pubmed publisher
Lucas E, Schafer J, Matsuda J, Kraus M, Burchill M, Tamburini B. PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity. Cell Rep. 2020;33:108258 pubmed publisher
Tuganbaev T, Mor U, Bashiardes S, Liwinski T, Nobs S, Leshem A, et al. Diet Diurnally Regulates Small Intestinal Microbiome-Epithelial-Immune Homeostasis and Enteritis. Cell. 2020;: pubmed publisher
McCauley M, O Rourke J, Yáñez A, Markman J, Ho R, Wang X, et al. C9orf72 in myeloid cells suppresses STING-induced inflammation. Nature. 2020;585:96-101 pubmed publisher
Peng L, Wang Y, Fei S, Wei C, Tong F, Wu G, et al. The effect of combining Endostar with radiotherapy on blood vessels, tumor-associated macrophages, and T cells in brain metastases of Lewis lung cancer. Transl Lung Cancer Res. 2020;9:745-760 pubmed publisher
Utley A, Chavel C, Lightman S, Holling G, Cooper J, Peng P, et al. CD28 Regulates Metabolic Fitness for Long-Lived Plasma Cell Survival. Cell Rep. 2020;31:107815 pubmed publisher
Yuan C, Yang D, Ma J, Yang J, Xue J, Song F, et al. Modulation of Wnt/β-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells. Braz J Med Biol Res. 2020;53:e9488 pubmed publisher
Wang H, Sobral M, Zhang D, Cartwright A, Li A, Dellacherie M, et al. Metabolic labeling and targeted modulation of dendritic cells. Nat Mater. 2020;19:1244-1252 pubmed publisher
Lv Z, Zhang P, Li D, Qin M, Nie L, Wang X, et al. CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models. Oncoimmunology. 2020;9:1747688 pubmed publisher
Gupta P, Johns S, Kim S, El Ghazal R, Zuniga E, Fuster M. Functional Cellular Anti-Tumor Mechanisms are Augmented by Genetic Proteoglycan Targeting. Neoplasia. 2020;22:86-97 pubmed publisher
Xie Y, Peng J, Pang J, Guo K, Zhang L, Yin S, et al. Biglycan regulates neuroinflammation by promoting M1 microglial activation in early brain injury after experimental subarachnoid hemorrhage. J Neurochem. 2020;152:368-380 pubmed publisher
Lauterbach M, Hanke J, Serefidou M, Mangan M, Kolbe C, Hess T, et al. Toll-like Receptor Signaling Rewires Macrophage Metabolism and Promotes Histone Acetylation via ATP-Citrate Lyase. Immunity. 2019;51:997-1011.e7 pubmed publisher
Horiguchi H, Kadomatsu T, Kurahashi R, Hara C, Miyata K, Baba M, et al. Dual functions of angiopoietin-like protein 2 signaling in tumor progression and anti-tumor immunity. Genes Dev. 2019;33:1641-1656 pubmed publisher
Koyama M, Mukhopadhyay P, Schuster I, Henden A, Hülsdünker J, Varelias A, et al. MHC Class II Antigen Presentation by the Intestinal Epithelium Initiates Graft-versus-Host Disease and Is Influenced by the Microbiota. Immunity. 2019;51:885-898.e7 pubmed publisher
Kodumudi K, Ramamoorthi G, Snyder C, Basu A, Jia Y, Awshah S, et al. Sequential Anti-PD1 Therapy Following Dendritic Cell Vaccination Improves Survival in a HER2 Mammary Carcinoma Model and Identifies a Critical Role for CD4 T Cells in Mediating the Response. Front Immunol. 2019;10:1939 pubmed publisher
Grabinger T, Glaus Garzon J, Hausmann M, Geirnaert A, Lacroix C, Hennet T. Alleviation of Intestinal Inflammation by Oral Supplementation With 2-Fucosyllactose in Mice. Front Microbiol. 2019;10:1385 pubmed publisher
Bell C, Fennell K, Chan Y, Rambow F, Yeung M, Vassiliadis D, et al. Targeting enhancer switching overcomes non-genetic drug resistance in acute myeloid leukaemia. Nat Commun. 2019;10:2723 pubmed publisher
Lightman S, Utley A, Lee K. Survival of Long-Lived Plasma Cells (LLPC): Piecing Together the Puzzle. Front Immunol. 2019;10:965 pubmed publisher
Wang S, Gao J, Li M, Wang L, Wang Z. A facile approach for development of a vaccine made of bacterial double-layered membrane vesicles (DMVs). Biomaterials. 2018;187:28-38 pubmed publisher
Kramer K, Young S, Walker G. Comparative Study of 5'- and 3'-Linked CpG-Antigen Conjugates for the Induction of Cellular Immune Responses. ACS Omega. 2017;2:227-235 pubmed publisher
Jiang H, Zhang S, Song T, Guan X, Zhang R, Chen X. Trichostatin a Protects Dendritic Cells Against Oxygen-Glucose Deprivation via the SRSF3/PKM2/Glycolytic Pathway. Front Pharmacol. 2018;9:612 pubmed publisher
Kimura A, Kitajima M, Nishida K, Serada S, Fujimoto M, Naka T, et al. NQO1 inhibits the TLR-dependent production of selective cytokines by promoting IκB-ζ degradation. J Exp Med. 2018;215:2197-2209 pubmed publisher
Sun T, Wang F, Pan W, Wu Q, Wang J, Dai J. An Immunosuppressive Tick Salivary Gland Protein DsCystatin Interferes With Toll-Like Receptor Signaling by Downregulating TRAF6. Front Immunol. 2018;9:1245 pubmed publisher
Nam G, Lee E, Kim Y, Hong Y, Choi Y, Ryu M, et al. Combined Rho-kinase inhibition and immunogenic cell death triggers and propagates immunity against cancer. Nat Commun. 2018;9:2165 pubmed publisher
Zhang Y, Chen P, Di G, Qi X, Zhou Q, Gao H. Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor. Sci Rep. 2018;8:5994 pubmed publisher
Barbet G, Sander L, Geswell M, Leonardi I, Cerutti A, Iliev I, et al. Sensing Microbial Viability through Bacterial RNA Augments T Follicular Helper Cell and Antibody Responses. Immunity. 2018;48:584-598.e5 pubmed publisher
Ye Y, Wang C, Zhang X, Hu Q, Zhang Y, Liu Q, et al. A melanin-mediated cancer immunotherapy patch. Sci Immunol. 2017;2: pubmed publisher
Khare P, Challa D, Devanaboyina S, Velmurugan R, Hughes S, Greenberg B, et al. Myelin oligodendrocyte glycoprotein-specific antibodies from multiple sclerosis patients exacerbate disease in a humanized mouse model. J Autoimmun. 2018;86:104-115 pubmed publisher
Varol D, Mildner A, Blank T, Shemer A, Barashi N, Yona S, et al. Dicer Deficiency Differentially Impacts Microglia of the Developing and Adult Brain. Immunity. 2017;46:1030-1044.e8 pubmed publisher
Miura N, Akita H, Tateshita N, Nakamura T, Harashima H. Modifying Antigen-Encapsulating Liposomes with KALA Facilitates MHC Class I Antigen Presentation and Enhances Anti-tumor Effects. Mol Ther. 2017;25:1003-1013 pubmed publisher
Kramer K, Shields N, Poppe V, Young S, Walker G. Intracellular Cleavable CpG Oligodeoxynucleotide-Antigen Conjugate Enhances Anti-tumor Immunity. Mol Ther. 2017;25:62-70 pubmed publisher
Sebina I, James K, Soon M, Fogg L, Best S, Labastida Rivera F, et al. IFNAR1-Signalling Obstructs ICOS-mediated Humoral Immunity during Non-lethal Blood-Stage Plasmodium Infection. PLoS Pathog. 2016;12:e1005999 pubmed publisher
Spinner C, Uttenweiler Joseph S, Metais A, Stella A, Burlet Schiltz O, Moog Lutz C, et al. Substrates of the ASB2α E3 ubiquitin ligase in dendritic cells. Sci Rep. 2015;5:16269 pubmed publisher
Moreau J, Berger A, Nelles M, Mielnik M, Furlonger C, Cen S, et al. Inflammation rapidly reorganizes mouse bone marrow B cells and their environment in conjunction with early IgM responses. Blood. 2015;126:1184-92 pubmed publisher
Fu H, Kishore M, Gittens B, Wang G, Coe D, Komarowska I, et al. Self-recognition of the endothelium enables regulatory T-cell trafficking and defines the kinetics of immune regulation. Nat Commun. 2014;5:3436 pubmed publisher
Xia S, Wei J, Wang J, Sun H, Zheng W, Li Y, et al. A requirement of dendritic cell-derived interleukin-27 for the tumor infiltration of regulatory T cells. J Leukoc Biol. 2014;95:733-742 pubmed
Klinger M, Dattilio A, Vizzard M. Expression of cyclooxygenase-2 in urinary bladder in rats with cyclophosphamide-induced cystitis. Am J Physiol Regul Integr Comp Physiol. 2007;293:R677-85 pubmed
Shih F, Racz J, Allen P. Differential MHC class II presentation of a pathogenic autoantigen during health and disease. J Immunol. 2006;176:3438-48 pubmed
product information
Antigen :
CD86
Apps. Abbrev. :
Flow Cytometry
Cat # :
105007
Clone :
GL-1
Item :
PE anti-mouse CD86
Isotype :
Rat IgG2a, κ
Other Names :
B7-2, B70, Ly-58
Size :
50 μg
Price (USD) :
110 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
LPS-activated CBA/Ca mouse splenic B cells
Application Notes :
The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , immunohistochemical staining of acetone-fixed frozen sections 2,6 , immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses 1-6 . GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105051-105056).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA