product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD80
catalog :
104716
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
hamsters
conjugate :
AF488
clone name :
16-10A1
reactivity :
mouse, dogs
application :
flow cytometry, mass cytometry
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Neumann S, Campbell K, Woodall M, Evans M, Clarkson A, Young S. Obesity Has a Systemic Effect on Immune Cells in Naïve and Cancer-Bearing Mice. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; fig 6c
Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
  • flow cytometry; mouse
Zhao L, Hu S, Davila M, Yang J, Lin Y, Albanese J, et al. Coordinated co-migration of CCR10+ antibody-producing B cells with helper T cells for colonic homeostatic regulation. Mucosal Immunol. 2021;14:420-430 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5h
Chen J, Cao X, Li B, Zhao Z, Chen S, Lai S, et al. Warburg Effect Is a Cancer Immune Evasion Mechanism Against Macrophage Immunosurveillance. Front Immunol. 2020;11:621757 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Cignarella F, Filipello F, Bollman B, Cantoni C, Locca A, Mikesell R, et al. TREM2 activation on microglia promotes myelin debris clearance and remyelination in a model of multiple sclerosis. Acta Neuropathol. 2020;140:513-534 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2g
Chao Y, Liang C, Tao H, Du Y, Wu D, Dong Z, et al. Localized cocktail chemoimmunotherapy after in situ gelation to trigger robust systemic antitumor immune responses. Sci Adv. 2020;6:eaaz4204 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1f, s2b
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • mass cytometry; mouse; loading ...; fig 5d
Martinez L, Garcia G, Contreras D, Gong D, Sun R, Arumugaswami V. Zika Virus Mucosal Infection Provides Protective Immunity. J Virol. 2020;94: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, 2d
Canel M, Taggart D, Sims A, Lonergan D, Waizenegger I, Serrels A. T-cell co-stimulation in combination with targeting FAK drives enhanced anti-tumor immunity. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 1a
Koike T, Harada K, Horiuchi S, Kitamura D. The quantity of CD40 signaling determines the differentiation of B cells into functionally distinct memory cell subsets. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse; fig 4b
Sivaram N, McLaughlin P, Han H, Petrenko O, Jiang Y, Ballou L, et al. Tumor-intrinsic PIK3CA represses tumor immunogenecity in a model of pancreatic cancer. J Clin Invest. 2019;130: pubmed publisher
  • flow cytometry; mouse; fig 3b
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Montero Herradón S, García Ceca J, Zapata A. Altered Maturation of Medullary TEC in EphB-Deficient Thymi Is Recovered by RANK Signaling Stimulation. Front Immunol. 2018;9:1020 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1
Mencarelli A, Khameneh H, Fric J, Vacca M, El Daker S, Janela B, et al. Calcineurin-mediated IL-2 production by CD11chighMHCII+ myeloid cells is crucial for intestinal immune homeostasis. Nat Commun. 2018;9:1102 pubmed publisher
  • flow cytometry; mouse; fig s6d
Pedros C, Canonigo Balancio A, Kong K, Altman A. Requirement of Treg-intrinsic CTLA4/PKCη signaling pathway for suppressing tumor immunity. JCI Insight. 2017;2: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5c
Akiel M, Guo C, Li X, Rajasekaran D, Mendoza R, Robertson C, et al. IGFBP7 Deletion Promotes Hepatocellular Carcinoma. Cancer Res. 2017;77:4014-4025 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2b
Kogo H, Shimizu M, Negishi Y, Uchida E, Takahashi H. Suppression of murine tumour growth through CD8+ cytotoxic T lymphocytes via activated DEC-205+ dendritic cells by sequential administration of ?-galactosylceramide in vivo. Immunology. 2017;151:324-339 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Hammer A, Yang G, Friedrich J, Kovacs A, Lee D, Grave K, et al. Role of the receptor Mas in macrophage-mediated inflammation in vivo. Proc Natl Acad Sci U S A. 2016;113:14109-14114 pubmed
  • flow cytometry; mouse; 1:400; loading ...; fig 1a
Kouwenberg M, Jacobs C, van der Vlag J, Hilbrands L. Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype. PLoS ONE. 2016;11:e0159986 pubmed publisher
Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
Wong R, Belk J, Govero J, Uhrlaub J, Reinartz D, Zhao H, et al. Affinity-Restricted Memory B Cells Dominate Recall Responses to Heterologous Flaviviruses. Immunity. 2020;53:1078-1094.e7 pubmed publisher
Liang J, Wang H, Ding W, Huang J, Zhou X, Wang H, et al. Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity. Sci Adv. 2020;6:eabc3646 pubmed publisher
Wang L, Zhang H, Sun L, Gao W, Xiong Y, Ma A, et al. Manipulation of macrophage polarization by peptide-coated gold nanoparticles and its protective effects on acute lung injury. J Nanobiotechnology. 2020;18:38 pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Hornick E, Dagvadorj J, Zacharias Z, Miller A, Langlois R, Chen P, et al. Dendritic cell NLRC4 regulates influenza A virus-specific CD4 T cell responses through FasL expression. J Clin Invest. 2019;129:2888-2897 pubmed publisher
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
Uchil P, Pi R, Haugh K, Ladinsky M, Ventura J, Barrett B, et al. A Protective Role for the Lectin CD169/Siglec-1 against a Pathogenic Murine Retrovirus. Cell Host Microbe. 2019;25:87-100.e10 pubmed publisher
Ding L, Kim H, Wang Q, Kearns M, Jiang T, Ohlson C, et al. PARP Inhibition Elicits STING-Dependent Antitumor Immunity in Brca1-Deficient Ovarian Cancer. Cell Rep. 2018;25:2972-2980.e5 pubmed publisher
Vega Angeles V, Terrazas L, Ledesma Soto Y, Jimenez L, Landa A. Taenia solium glutathione transferase fraction activates macrophages and favors the development of Th1-type response. Biosci Rep. 2019;39: pubmed publisher
Kiyohara H, Sujino T, Teratani T, Miyamoto K, Arai M, Nomura E, et al. Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. Cell Mol Gastroenterol Hepatol. 2019;7:135-156 pubmed publisher
Liang Y, Tang H, Guo J, Qiu X, Yang Z, Ren Z, et al. Targeting IFNα to tumor by anti-PD-L1 creates feedforward antitumor responses to overcome checkpoint blockade resistance. Nat Commun. 2018;9:4586 pubmed publisher
Imai T, Matsumura T, Mayer Lambertz S, Wells C, Ishikawa E, Butcher S, et al. Lipoteichoic acid anchor triggers Mincle to drive protective immunity against invasive group A Streptococcus infection. Proc Natl Acad Sci U S A. 2018;115:E10662-E10671 pubmed publisher
Payne K, Aqbi H, Butler S, Graham L, Keim R, Wan W, et al. Gr1-/low CD11b-/low MHCII+ myeloid cells boost T cell anti-tumor efficacy. J Leukoc Biol. 2018;104:1215-1228 pubmed publisher
Chaganty B, Qiu S, Gest A, Lu Y, Ivan C, Calin G, et al. Trastuzumab upregulates PD-L1 as a potential mechanism of trastuzumab resistance through engagement of immune effector cells and stimulation of IFN? secretion. Cancer Lett. 2018;430:47-56 pubmed publisher
Tung S, Boardman D, Sen M, Letizia M, Peng Q, Cianci N, et al. Regulatory T cell-derived extracellular vesicles modify dendritic cell function. Sci Rep. 2018;8:6065 pubmed publisher
Watanabe K, Luo Y, Da T, Guedan S, Ruella M, Scholler J, et al. Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses. JCI Insight. 2018;3: pubmed publisher
Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell. 2018;173:634-648.e12 pubmed publisher
Chen Y, Chaudhary N, Yang N, Granato A, Turner J, Howard S, et al. Microbial symbionts regulate the primary Ig repertoire. J Exp Med. 2018;215:1397-1415 pubmed publisher
Mayordomo A, Silva J, Gorlino C, Arias J, Berón W, Di Genaro M. IL-12/23p40 overproduction by dendritic cells leads to an increased Th1 and Th17 polarization in a model of Yersinia enterocolitica-induced reactive arthritis in TNFRp55-/- mice. PLoS ONE. 2018;13:e0193573 pubmed publisher
Perry C, Muñoz Rojas A, Meeth K, Kellman L, Amezquita R, Thakral D, et al. Myeloid-targeted immunotherapies act in synergy to induce inflammation and antitumor immunity. J Exp Med. 2018;215:877-893 pubmed publisher
Kitamura T, Doughty Shenton D, Cassetta L, Fragkogianni S, Brownlie D, Kato Y, et al. Monocytes Differentiate to Immune Suppressive Precursors of Metastasis-Associated Macrophages in Mouse Models of Metastatic Breast Cancer. Front Immunol. 2017;8:2004 pubmed publisher
Cowan J, Baik S, McCarthy N, Parnell S, White A, Jenkinson W, et al. Aire controls the recirculation of murine Foxp3+ regulatory T-cells back to the thymus. Eur J Immunol. 2018;48:844-854 pubmed publisher
Xenakis J, Howard E, Smith K, Olbrich C, Huang Y, Anketell D, et al. Resident intestinal eosinophils constitutively express antigen presentation markers and include two phenotypically distinct subsets of eosinophils. Immunology. 2018;154:298-308 pubmed publisher
Chmielewski M, Abken H. CAR T Cells Releasing IL-18 Convert to T-Bethigh FoxO1low Effectors that Exhibit Augmented Activity against Advanced Solid Tumors. Cell Rep. 2017;21:3205-3219 pubmed publisher
Wang Q, Racine J, Ratiu J, Wang S, Ettinger R, Wasserfall C, et al. Transient BAFF Blockade Inhibits Type 1 Diabetes Development in Nonobese Diabetic Mice by Enriching Immunoregulatory B Lymphocytes Sensitive to Deletion by Anti-CD20 Cotherapy. J Immunol. 2017;199:3757-3770 pubmed publisher
Roussey J, Viglianti S, Teitz Tennenbaum S, Olszewski M, Osterholzer J. Anti-PD-1 Antibody Treatment Promotes Clearance of Persistent Cryptococcal Lung Infection in Mice. J Immunol. 2017;199:3535-3546 pubmed publisher
Jain R, Sheridan J, Policheni A, Heinlein M, Gandolfo L, Dewson G, et al. A critical epithelial survival axis regulated by MCL-1 maintains thymic function in mice. Blood. 2017;130:2504-2515 pubmed publisher
Capece T, Walling B, Lim K, Kim K, Bae S, Chung H, et al. A novel intracellular pool of LFA-1 is critical for asymmetric CD8+ T cell activation and differentiation. J Cell Biol. 2017;216:3817-3829 pubmed publisher
Cosway E, LUCAS B, James K, Parnell S, Carvalho Gaspar M, White A, et al. Redefining thymus medulla specialization for central tolerance. J Exp Med. 2017;214:3183-3195 pubmed publisher
Espinosa Cueto P, Magallanes Puebla A, Castellanos C, Mancilla R. Dendritic cells that phagocytose apoptotic macrophages loaded with mycobacterial antigens activate CD8 T cells via cross-presentation. PLoS ONE. 2017;12:e0182126 pubmed publisher
Ki S, Thyagarajan H, Hu Z, Lancaster J, Ehrlich L. EBI2 contributes to the induction of thymic central tolerance in mice by promoting rapid motility of medullary thymocytes. Eur J Immunol. 2017;47:1906-1917 pubmed publisher
Gmyrek G, Pingel J, Choi J, Green J. Functional analysis of acquired CD28 mutations identified in cutaneous T cell lymphoma. Cell Immunol. 2017;319:28-34 pubmed publisher
White A, Baik S, Parnell S, Holland A, Brombacher F, Jenkinson W, et al. A type 2 cytokine axis for thymus emigration. J Exp Med. 2017;214:2205-2216 pubmed publisher
Vijay R, Fehr A, Janowski A, Athmer J, Wheeler D, Grunewald M, et al. Virus-induced inflammasome activation is suppressed by prostaglandin D2/DP1 signaling. Proc Natl Acad Sci U S A. 2017;114:E5444-E5453 pubmed publisher
Parashar K, Kopping E, Frank D, Sampath V, Thanassi D, Carpino N. Increased Resistance to Intradermal Francisella tularensis LVS Infection by Inactivation of the Sts Phosphatases. Infect Immun. 2017;85: pubmed publisher
Ratiu J, Racine J, Hasham M, Wang Q, Branca J, Chapman H, et al. Genetic and Small Molecule Disruption of the AID/RAD51 Axis Similarly Protects Nonobese Diabetic Mice from Type 1 Diabetes through Expansion of Regulatory B Lymphocytes. J Immunol. 2017;198:4255-4267 pubmed publisher
Nagata M, Izumi Y, Ishikawa E, Kiyotake R, Doi R, Iwai S, et al. Intracellular metabolite ?-glucosylceramide is an endogenous Mincle ligand possessing immunostimulatory activity. Proc Natl Acad Sci U S A. 2017;114:E3285-E3294 pubmed publisher
von Rohrscheidt J, Petrozziello E, Nedjic J, Federle C, Krzyzak L, Ploegh H, et al. Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83. J Exp Med. 2016;213:1685-94 pubmed publisher
Akiyama N, Takizawa N, Miyauchi M, Yanai H, Tateishi R, Shinzawa M, et al. Identification of embryonic precursor cells that differentiate into thymic epithelial cells expressing autoimmune regulator. J Exp Med. 2016;213:1441-58 pubmed publisher
Mohammed J, Beura L, Bobr A, Astry B, Chicoine B, Kashem S, et al. Stromal cells control the epithelial residence of DCs and memory T cells by regulated activation of TGF-β. Nat Immunol. 2016;17:414-21 pubmed publisher
White A, Jenkinson W, Cowan J, Parnell S, Bacon A, Jones N, et al. An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells. J Immunol. 2014;192:2659-66 pubmed publisher
Philipsen L, Engels T, Schilling K, Gurbiel S, Fischer K, Tedford K, et al. Multimolecular analysis of stable immunological synapses reveals sustained recruitment and sequential assembly of signaling clusters. Mol Cell Proteomics. 2013;12:2551-67 pubmed publisher
del Rio M, Cote Sierra J, Rodriguez Barbosa J. Flt3L-mobilized dendritic cells bearing H2-Kbm1 apoptotic cells do not induce cross-tolerance to CD8+ T cells across a class I MHC mismatched barrier. Transpl Int. 2011;24:501-13 pubmed publisher
Misra R, Shi G, Moreno Garcia M, Thankappan A, Tighe M, Mousseau B, et al. G alpha q-containing G proteins regulate B cell selection and survival and are required to prevent B cell-dependent autoimmunity. J Exp Med. 2010;207:1775-89 pubmed publisher
product information
Antigen :
CD80
Apps. Abbrev. :
Flow Cytometry
Cat # :
104716
Clone :
16-10A1
Item :
Alexa Fluor® 488 anti-mouse CD80
Isotype :
Armenian Hamster IgG
Other Names :
B7-1, B7, Ly-53
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse, Cross-Reactivity: Dog (Canine)
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
A488
Immunogen :
CHO cell line transfected with mouse B7 (CD80)
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 2 , in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells 2-4 , and immunohistochemical staining of acetone-fixed frozen sections 1,4 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. Nos. 104747-104752).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA