LEAF™ Purified anti-mouse CD70 | BioLegend 104608 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

LEAF™ Purified anti-mouse CD70

catalog :

104608

quantity :

500 μg

price :

265 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

FR70

reactivity :

mouse

application :

flow cytometry

citations: 2

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig s15d	Sugiura D, Maruhashi T, Okazaki I, Shimizu K, Maeda T, Takemoto T, et al. Restriction of PD-1 function by cis-PD-L1/CD80 interactions is required for optimal T cell responses. Science. 2019;364:558-566 pubmed publisher
flow cytometry; mouse; loading ...; fig 7a	McKinstry K, Strutt T, Bautista B, Zhang W, Kuang Y, Cooper A, et al. Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2. Nat Commun. 2014;5:5377 pubmed publisher

product information

Antigen :

CD70

Apps. Abbrev. :

Flow Cytometry

Cat # :

104608

Clone :

FR70

Item :

LEAF™ Purified anti-mouse CD70

Isotype :

Rat IgG2b, κ

Other Names :

Ki-24, CD27-ligand, TNFSF7

Size :

500 μg

Price (USD) :

265 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

LEAF

Immunogen :

BALB/c mouse B lymphoma A20.2J

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses 1-3 , and immunohistochemistry 4 of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 104605/104606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 104604) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin 0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 104608).