Biotin anti-mouse CD70 | BioLegend 104603 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Biotin anti-mouse CD70

catalog :

104603

quantity :

50 μg

price :

83 USD

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

FR70

reactivity :

mouse

application :

flow cytometry

more info or order :

BioLegend product webpage

citations: 2

Reference
Wang K, Chu K, Batista N, Watts T. Conserved and Differential Features of TNF Superfamily Ligand Expression on APC Subsets over the Course of a Chronic Viral Infection in Mice. Immunohorizons. 2018;2:407-417 pubmed publisher
Chang Y, Wang K, Chu K, Clouthier D, Tran A, Torres Perez M, et al. Dichotomous Expression of TNF Superfamily Ligands on Antigen-Presenting Cells Controls Post-priming Anti-viral CD4+ T Cell Immunity. Immunity. 2017;47:943-958.e9 pubmed publisher

product information

Antigen :

CD70

Apps. Abbrev. :

Flow Cytometry

Cat # :

104603

Clone :

FR70

Item :

Biotin anti-mouse CD70

Isotype :

Rat IgG2b, κ

Other Names :

Ki-24, CD27-ligand, TNFSF7

Size :

50 μg

Price (USD) :

83 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

BIOTIN

Immunogen :

BALB/c mouse B lymphoma A20.2J

Application Notes :

Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro blocking of CD70-CD27 binding and inhibition of T and NK cell responses 1-3 , and immunohistochemistry 4 of aceton-fixed frozen sections. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 104605/104606) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 104604) or biotinylated anti-rat IgG (Cat. No. 405402) second step, followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. The LEAF™ purified antibody (Endotoxin 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104608).

more info or order :

BioLegend product webpage