product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Spark NIR™ 685 anti-mouse CD69
catalog :
104557
quantity :
25 μg
price :
112 USD
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
H1.2F3
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 24
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4d
Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d, 6b, s6i
Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1b
Liu H, Pedros C, Kong K, Canonigo Balancio A, Xue W, Altman A. Leveraging the Treg-intrinsic CTLA4-PKCη signaling pathway for cancer immunotherapy. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...
Winn N, Wolf E, Cottam M, Bhanot M, Hasty A. Myeloid-specific deletion of ferroportin impairs macrophage bioenergetics but is disconnected from systemic insulin action in adult mice. Am J Physiol Endocrinol Metab. 2021;321:E376-E391 pubmed publisher
  • flow cytometry; mouse; fig 1e-h
  • flow cytometry; human; loading ...
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Amoozgar Z, Kloepper J, Ren J, Tay R, Kazer S, Kiner E, et al. Targeting Treg cells with GITR activation alleviates resistance to immunotherapy in murine glioblastomas. Nat Commun. 2021;12:2582 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5d, 5e
Li J, Xu J, Li Z. Obatoclax, the pan-Bcl-2 inhibitor sensitizes hepatocellular carcinoma cells to promote the anti-tumor efficacy in combination with immune checkpoint blockade. Transl Oncol. 2021;14:101116 pubmed publisher
  • flow cytometry; mouse; fig 2c
Can xe8 S, Van Snick J, Uyttenhove C, Pilotte L, van den Eynde B. TGFβ1 neutralization displays therapeutic efficacy through both an immunomodulatory and a non-immune tumor-intrinsic mechanism. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Brownlie D, Doughty Shenton D, Yh Soong D, Nixon C, O Carragher N, M Carlin L, et al. Metastasis-associated macrophages constrain antitumor capability of natural killer cells in the metastatic site at least partially by membrane bound transforming growth factor β. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig 3c
Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 7a, 7b
Pariser D, Hilt Z, Ture S, Blick Nitko S, Looney M, Cleary S, et al. Lung megakaryocytes are immune modulatory cells. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3g
Bekeschus S, Clemen R, Nießner F, Sagwal S, Freund E, Schmidt A. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion. Adv Sci (Weinh). 2020;7:1903438 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2d
Li A, Herbst R, Canner D, Schenkel J, Smith O, Kim J, et al. IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development. Cell Rep. 2019;29:2998-3008.e8 pubmed publisher
Ayasoufi K, Wolf D, Namen S, Jin F, Tritz Z, Pfaller C, et al. Brain resident memory T cells rapidly expand and initiate neuroinflammatory responses following CNS viral infection. Brain Behav Immun. 2023;112:51-76 pubmed publisher
Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi Maharlooei M, Iuga A, et al. Systematic elucidation and pharmacological targeting of tumor-infiltrating regulatory T cell master regulators. Cancer Cell. 2023;41:933-949.e11 pubmed publisher
Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
Yan J, Allen S, McDonald E, Das I, Mak J, Liu L, et al. MAIT Cells Promote Tumor Initiation, Growth, and Metastases via Tumor MR1. Cancer Discov. 2019;: pubmed publisher
Zhao Y, Yang Q, Jin C, Feng Y, Xie S, Xie H, et al. Changes of CD103-expressing pulmonary CD4+ and CD8+ T cells in S. japonicum infected C57BL/6 mice. BMC Infect Dis. 2019;19:999 pubmed publisher
Krovi S, Kappler J, Marrack P, Gapin L. Inherent reactivity of unselected TCR repertoires to peptide-MHC molecules. Proc Natl Acad Sci U S A. 2019;116:22252-22261 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Vega Angeles V, Terrazas L, Ledesma Soto Y, Jimenez L, Landa A. Taenia solium glutathione transferase fraction activates macrophages and favors the development of Th1-type response. Biosci Rep. 2019;39: pubmed publisher
Petvises S, Periasamy P, O Neill H. MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis. BMC Immunol. 2018;19:21 pubmed publisher
Elderman M, Hugenholtz F, Belzer C, Boekschoten M, van Beek A, de Haan B, et al. Sex and strain dependent differences in mucosal immunology and microbiota composition in mice. Biol Sex Differ. 2018;9:26 pubmed publisher
product information
Antigen :
CD69
Apps. Abbrev. :
FC
Cat # :
104557
Clone :
H1.2F3
Item :
Spark NIR™ 685 anti-mouse CD69
Isotype :
Armenian Hamster IgG
Other Names :
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Size :
25 μg
Price (USD) :
112 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
SPKNIR_685
Immunogen :
Mouse dendritic epidermal T cell line Y245
Application Notes :
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation^1-3, immunohistochemistry^4,5, and immunoprecipitation^1.This antibody has been characterized in the literature as containing a lambda light chain.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA