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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD69
catalog :
104508
quantity :
200 μg
price :
275 USD
clonality :
monoclonal
host :
hamsters
conjugate :
PE
clone name :
H1.2F3
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 59
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 1e-h
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Barker K, Etesami N, Shenoy A, Arafa E, Lyon de Ana C, Smith N, et al. Lung-resident memory B cells protect against bacterial pneumonia. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a, s2b, s2c
Zheng W, Song H, Luo Z, Wu H, Chen L, Wang Y, et al. Acetylcholine ameliorates colitis by promoting IL-10 secretion of monocytic myeloid-derived suppressor cells through the nAChR/ERK pathway. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3
Glasner A, Isaacson B, Viukov S, Neuman T, Friedman N, Mandelboim M, et al. Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression. Sci Rep. 2017;7:13090 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig s1c
Guo Z, Kong Q, Liu C, Zhang S, Zou L, Yan F, et al. DCAF1 controls T-cell function via p53-dependent and -independent mechanisms. Nat Commun. 2016;7:10307 pubmed publisher
  • flow cytometry; mouse
Olguín J, Fernández J, Salinas N, Juárez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
Luo W, Napoleon J, Zhang F, Lee Y, Wang B, Putt K, et al. Repolarization of Tumor-Infiltrating Myeloid Cells for Augmentation of CAR T Cell Therapies. Front Immunol. 2022;13:816761 pubmed publisher
Lopes N, Boucherit N, Santamaria J, Provin N, Charaix J, Ferrier P, et al. Thymocytes trigger self-antigen-controlling pathways in immature medullary thymic epithelial stages. elife. 2022;11: pubmed publisher
Suryawanshi R, Patil C, Agelidis A, Koganti R, Ames J, Koujah L, et al. mTORC2 confers neuroprotection and potentiates immunity during virus infection. Nat Commun. 2021;12:6020 pubmed publisher
Afolabi L, Bi J, Li X, Adeshakin A, Adeshakin F, Wu H, et al. Synergistic Tumor Cytolysis by NK Cells in Combination With a Pan-HDAC Inhibitor, Panobinostat. Front Immunol. 2021;12:701671 pubmed publisher
van Geffen C, Deißler A, Beer Hammer S, Nurnberg B, Handgretinger R, Renz H, et al. Myeloid-Derived Suppressor Cells Dampen Airway Inflammation Through Prostaglandin E2 Receptor 4. Front Immunol. 2021;12:695933 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Daneshmandi S, Cassel T, Lin P, Higashi R, Wulf G, Boussiotis V, et al. Blockade of 6-phosphogluconate dehydrogenase generates CD8+ effector T cells with enhanced anti-tumor function. Cell Rep. 2021;34:108831 pubmed publisher
Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed publisher
Watanabe M, Lu Y, Breen M, Hodes R. B7-CD28 co-stimulation modulates central tolerance via thymic clonal deletion and Treg generation through distinct mechanisms. Nat Commun. 2020;11:6264 pubmed publisher
Corrado M, Edwards Hicks J, Villa M, Flachsmann L, Sanin D, Jacobs M, et al. Dynamic Cardiolipin Synthesis Is Required for CD8+ T Cell Immunity. Cell Metab. 2020;32:981-995.e7 pubmed publisher
León Letelier R, Castro Medina D, Badillo Godinez O, Tepale Segura A, Huanosta Murillo E, Aguilar Flores C, et al. Induction of Progenitor Exhausted Tissue-Resident Memory CD8+ T Cells Upon Salmonella Typhi Porins Adjuvant Immunization Correlates With Melanoma Control and Anti-PD-1 Immunotherapy Cooperation. Front Immunol. 2020;11:583382 pubmed publisher
Walter F, Winter E, Rahn S, Heidland J, Meier S, Struzek A, et al. Chitosan nanoparticles as antigen vehicles to induce effective tumor specific T cell responses. PLoS ONE. 2020;15:e0239369 pubmed publisher
Wu J, Madi A, Mieg A, Hotz Wagenblatt A, Weisshaar N, Ma S, et al. T Cell Factor 1 Suppresses CD103+ Lung Tissue-Resident Memory T Cell Development. Cell Rep. 2020;31:107484 pubmed publisher
Han P, Hanlon D, Arshad N, Lee J, Tatsuno K, Robinson E, et al. Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Sci Adv. 2020;6:eaaz1580 pubmed publisher
D Alessandro G, Antonangeli F, Marrocco F, Porzia A, Lauro C, Santoni A, et al. Gut microbiota alterations affect glioma growth and innate immune cells involved in tumor immunosurveillance in mice. Eur J Immunol. 2020;50:705-711 pubmed publisher
Sendler M, van den Brandt C, Glaubitz J, Wilden A, Golchert J, Weiss F, et al. NLRP3 Inflammasome Regulates Development of Systemic Inflammatory Response and Compensatory Anti-Inflammatory Response Syndromes in Mice With Acute Pancreatitis. Gastroenterology. 2020;158:253-269.e14 pubmed publisher
Leylek R, Alcántara Hernández M, Lanzar Z, Lüdtke A, Perez O, Reizis B, et al. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population. Cell Rep. 2019;29:3736-3750.e8 pubmed publisher
Jackstadt R, van Hooff S, Leach J, Cortés Lavaud X, Lohuis J, Ridgway R, et al. Epithelial NOTCH Signaling Rewires the Tumor Microenvironment of Colorectal Cancer to Drive Poor-Prognosis Subtypes and Metastasis. Cancer Cell. 2019;36:319-336.e7 pubmed publisher
Contreras N, Sitnik K, Jeftic I, Coplen C, Cicin Sain L, Nikolich Zugich J. Life-long control of cytomegalovirus (CMV) by T resident memory cells in the adipose tissue results in inflammation and hyperglycemia. PLoS Pathog. 2019;15:e1007890 pubmed publisher
Skadow M, Penna V, Galant Swafford J, Shevach E, Thornton A. Helios Deficiency Predisposes the Differentiation of CD4+Foxp3- T Cells into Peripherally Derived Regulatory T Cells. J Immunol. 2019;: pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
Hu Q, Sun W, Wang J, Ruan H, Zhang X, Ye Y, et al. Conjugation of haematopoietic stem cells and platelets decorated with anti-PD-1 antibodies augments anti-leukaemia efficacy. Nat Biomed Eng. 2018;2:831-840 pubmed publisher
Misumi I, Starmer J, Uchimura T, Beck M, Magnuson T, Whitmire J. Obesity Expands a Distinct Population of T Cells in Adipose Tissue and Increases Vulnerability to Infection. Cell Rep. 2019;27:514-524.e5 pubmed publisher
Kemp V, van den Wollenberg D, Camps M, van Hall T, Kinderman P, Pronk van Montfoort N, et al. Arming oncolytic reovirus with GM-CSF gene to enhance immunity. Cancer Gene Ther. 2018;: pubmed publisher
Subramanian K, Neill D, Malak H, Spelmink L, Khandaker S, Dalla Libera Marchiori G, et al. Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival. Nat Microbiol. 2019;4:62-70 pubmed publisher
Tsai S, Clemente Casares X, Zhou A, Lei H, Ahn J, Chan Y, et al. Insulin Receptor-Mediated Stimulation Boosts T Cell Immunity during Inflammation and Infection. Cell Metab. 2018;28:922-934.e4 pubmed publisher
Caronni N, Simoncello F, Stafetta F, Guarnaccia C, Ruiz Moreno J, Opitz B, et al. Downregulation of Membrane Trafficking Proteins and Lactate Conditioning Determine Loss of Dendritic Cell Function in Lung Cancer. Cancer Res. 2018;78:1685-1699 pubmed publisher
Koh A, Miller E, Buenrostro J, Moskowitz D, Wang J, Greenleaf W, et al. Rapid chromatin repression by Aire provides precise control of immune tolerance. Nat Immunol. 2018;19:162-172 pubmed publisher
Toubai T, Rossi C, Oravecz Wilson K, Liu C, Zajac C, Wu S, et al. IAPs protect host target tissues from graft-versus-host disease in mice. Blood Adv. 2017;1:1517-1532 pubmed publisher
Place D, Williamson D, Yuzefpolskiy Y, Katkere B, Sarkar S, Kalia V, et al. Development of a novel Francisella tularensis Live Vaccine Strain expressing ovalbumin provides insight into antigen-specific CD8+ T cell responses. PLoS ONE. 2017;12:e0190384 pubmed publisher
Amantini C, Farfariello V, Cardinali C, Morelli M, Marinelli O, Nabissi M, et al. The TRPV1 ion channel regulates thymocyte differentiation by modulating autophagy and proteasome activity. Oncotarget. 2017;8:90766-90780 pubmed publisher
Palazon A, Tyrakis P, Macías D, Veliça P, Rundqvist H, Fitzpatrick S, et al. An HIF-1α/VEGF-A Axis in Cytotoxic T Cells Regulates Tumor Progression. Cancer Cell. 2017;32:669-683.e5 pubmed publisher
Yan Q, Yang C, Fu Q, Chen Z, Liu S, Fu D, et al. Scaffold protein JLP mediates TCR-initiated CD4+T cell activation and CD154 expression. Mol Immunol. 2017;87:258-266 pubmed publisher
Younan P, Ramanathan P, Graber J, Gusovsky F, Bukreyev A. The Toll-Like Receptor 4 Antagonist Eritoran Protects Mice from Lethal Filovirus Challenge. MBio. 2017;8: pubmed publisher
Mier Aguilar C, Cashman K, Raman C, Soldevila G. CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival. PLoS ONE. 2016;11:e0168155 pubmed publisher
Velazquez K, Enos R, Carson M, Cranford T, Bader J, Chatzistamou I, et al. Weight loss following diet-induced obesity does not alter colon tumorigenesis in the AOM mouse model. Am J Physiol Gastrointest Liver Physiol. 2016;311:G699-G712 pubmed publisher
Cerina M, Narayanan V, Göbel K, Bittner S, Ruck T, Meuth P, et al. The quality of cortical network function recovery depends on localization and degree of axonal demyelination. Brain Behav Immun. 2017;59:103-117 pubmed publisher
Xu X, Wang X, Todd E, Jaeger E, Vella J, Mooren O, et al. Mst1 Kinase Regulates the Actin-Bundling Protein L-Plastin To Promote T Cell Migration. J Immunol. 2016;197:1683-91 pubmed publisher
Kang J, Lee J, Chang J. Cholera Toxin Promotes Th17 Cell Differentiation by Modulating Expression of Polarizing Cytokines and the Antigen-Presenting Potential of Dendritic Cells. PLoS ONE. 2016;11:e0157015 pubmed publisher
Artinger K, Kirsch A, Aringer I, Schabhüttl C, Rosenkranz A, Eller P, et al. The Spleen Plays No Role in Nephrotoxic Serum Nephritis, but Constitutes a Place of Compensatory Haematopoiesis. PLoS ONE. 2015;10:e0135087 pubmed publisher
Jang A, Song E, Shin S, Hwang P, Kim S, Jin Y, et al. Potentiation of natural killer (NK) cell activity by methanol extract of cultured cambial meristematic cells of wild ginseng and its mechanism. Life Sci. 2015;135:138-46 pubmed publisher
Sido J, Nagarkatti P, Nagarkatti M. Δ⁹-Tetrahydrocannabinol attenuates allogeneic host-versus-graft response and delays skin graft rejection through activation of cannabinoid receptor 1 and induction of myeloid-derived suppressor cells. J Leukoc Biol. 2015;98:435-47 pubmed publisher
Charlton J, Tsoukatou D, Mamalaki C, Chatzidakis I. Programmed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines. PLoS ONE. 2015;10:e0119200 pubmed publisher
Passos C, Cepeda M, Ferreira S, Nunes F, Evtuguin D, Madureira P, et al. Influence of molecular weight on in vitro immunostimulatory properties of instant coffee. Food Chem. 2014;161:60-6 pubmed publisher
Ishikawa C, Kawakami H, Uchihara J, Senba M, Mori N. CD69 overexpression by human T-cell leukemia virus type 1 Tax transactivation. Biochim Biophys Acta. 2013;1833:1542-52 pubmed publisher
Huang Z, Kang J, Chen C, Tseng T, Chang C, Chang Y, et al. Decoy receptor 3 suppresses TLR2-mediated B cell activation by targeting NF-?B. J Immunol. 2012;188:5867-76 pubmed publisher
Yuan Z, Zhang X, Lin R, Petersen E, He S, Yu M, et al. Protective effect against toxoplasmosis in mice induced by DNA immunization with gene encoding Toxoplasma gondii ROP18. Vaccine. 2011;29:6614-9 pubmed publisher
Kmieciak M, Worschech A, Nikizad H, Gowda M, Habibi M, Depcrynski A, et al. CD4+ T cells inhibit the neu-specific CD8+ T-cell exhaustion during the priming phase of immune responses against breast cancer. Breast Cancer Res Treat. 2011;126:385-94 pubmed publisher
Holan V, Pokorna K, Prochazkova J, Krulova M, Zajicova A. Immunoregulatory properties of mouse limbal stem cells. J Immunol. 2010;184:2124-9 pubmed publisher
Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
Epardaud M, Elpek K, Rubinstein M, Yonekura A, Bellemare Pelletier A, Bronson R, et al. Interleukin-15/interleukin-15R alpha complexes promote destruction of established tumors by reviving tumor-resident CD8+ T cells. Cancer Res. 2008;68:2972-83 pubmed publisher
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
product information
Antigen :
CD69
Apps. Abbrev. :
Flow Cytometry
Cat # :
104508
Clone :
H1.2F3
Item :
PE anti-mouse CD69
Isotype :
Armenian Hamster IgG
Other Names :
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Size :
200 μg
Price (USD) :
275 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
PE
Immunogen :
Mouse dendritic epidermal T cell line Y245
Application Notes :
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation 1-3 , immunohistochemistry 4,5 , and immunoprecipitation 1 . This antibody has been characterized in the literature as containing a lambda (?) light chain.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA