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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse CD69
catalog :
104506
quantity :
500 μg
price :
275 USD
clonality :
monoclonal
host :
hamsters
conjugate :
FITC
clone name :
H1.2F3
reactivity :
mouse
application :
immunohistochemistry, flow cytometry
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2a
Borkner L, Curham L, Wilk M, Moran B, Mills K. IL-17 mediates protective immunity against nasal infection with Bordetella pertussis by mobilizing neutrophils, especially Siglec-F+ neutrophils. Mucosal Immunol. 2021;14:1183-1202 pubmed publisher
  • flow cytometry; mouse; 1:250; loading ...; fig 4g
  • immunohistochemistry; mouse; loading ...; fig 2a
Bhaskar A, Kumar S, Khan M, Singh A, Dwivedi V, Nandicoori V. Host sirtuin 2 as an immunotherapeutic target against tuberculosis. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Yao Y, Huang W, Li X, Li X, Qian J, Han H, et al. Tespa1 Deficiency Dampens Thymus-Dependent B-Cell Activation and Attenuates Collagen-Induced Arthritis in Mice. Front Immunol. 2018;9:965 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Xiao F, Ai G, Yan W, Wan X, Luo X, Ning Q. Intrahepatic recruitment of cytotoxic NK cells contributes to autoimmune hepatitis progression. Cell Immunol. 2018;327:13-20 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Kogo H, Shimizu M, Negishi Y, Uchida E, Takahashi H. Suppression of murine tumour growth through CD8+ cytotoxic T lymphocytes via activated DEC-205+ dendritic cells by sequential administration of ?-galactosylceramide in vivo. Immunology. 2017;151:324-339 pubmed publisher
  • flow cytometry; mouse; loading ...; fig S1
Uhde A, Herder V, Akram Khan M, Ciurkiewicz M, Schaudien D, Teich R, et al. Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice. PLoS ONE. 2016;11:e0161883 pubmed publisher
  • flow cytometry; mouse; fig 1
Fontinha D, Lopes F, Marques S, Alenquer M, Simas J. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS ONE. 2015;10:e0142540 pubmed publisher
  • flow cytometry; mouse
White C, Villarino N, Sloan S, Ganusov V, Schmidt N. Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol. 2015;194:697-708 pubmed publisher
Wilson A, Randall K, Pettitt J, Ellyard J, Blumenthal A, Enders A, et al. Neutrophil extracellular traps and their histones promote Th17 cell differentiation directly via TLR2. Nat Commun. 2022;13:528 pubmed publisher
Gardner A, de Mingo Pulido Á, Hänggi K, Bazargan S, Onimus A, Kasprzak A, et al. TIM-3 blockade enhances IL-12-dependent antitumor immunity by promoting CD8+ T cell and XCR1+ dendritic cell spatial co-localization. J Immunother Cancer. 2022;10: pubmed publisher
Duan Q, Ding J, Li F, Liu X, Zhao Y, Yu H, et al. Sirtuin 5 is Dispensable for CD8+ T Cell Effector and Memory Differentiation. Front Cell Dev Biol. 2021;9:761193 pubmed publisher
Song T, Long M, Zhao H, Zou M, Fan H, Liu Y, et al. Tumor evolution selectively inactivates the core microRNA machinery for immune evasion. Nat Commun. 2021;12:7003 pubmed publisher
Rui J, Deng S, Perdigoto A, Ponath G, Kursawe R, Lawlor N, et al. Tet2 Controls the Responses of β cells to Inflammation in Autoimmune Diabetes. Nat Commun. 2021;12:5074 pubmed publisher
de Mingo Pulido Á, Hänggi K, Celias D, Gardner A, Li J, Batista Bittencourt B, et al. The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake. Immunity. 2021;54:1154-1167.e7 pubmed publisher
Mitchell J, Lund M, Starmer J, Ge K, Magnuson T, Shpargel K, et al. UTX promotes CD8+ T cell-mediated antiviral defenses but reduces T cell durability. Cell Rep. 2021;35:108966 pubmed publisher
Rashid M, Borin T, Ara R, Piranlioglu R, Achyut B, Korkaya H, et al. Critical immunosuppressive effect of MDSC‑derived exosomes in the tumor microenvironment. Oncol Rep. 2021;45:1171-1181 pubmed publisher
Tsyklauri O, Niederlova V, Forsythe E, Prasai A, Drobek A, Kaspárek P, et al. Bardet-Biedl Syndrome ciliopathy is linked to altered hematopoiesis and dysregulated self-tolerance. EMBO Rep. 2021;22:e50785 pubmed publisher
Byun J, Park M, Lee S, Yun J, Lee J, Kim J, et al. Inhibition of Glutamine Utilization Synergizes with Immune Checkpoint Inhibitor to Promote Antitumor Immunity. Mol Cell. 2020;80:592-606.e8 pubmed publisher
Wang N, Yi H, Fang L, Jin J, Ma Q, Shen Y, et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020;11:1883 pubmed publisher
Hasezaki T, Yoshima T, Mine Y. Anti-CD81 antibodies reduce migration of activated T lymphocytes and attenuate mouse experimental colitis. Sci Rep. 2020;10:6969 pubmed publisher
Demircioglu F, Wang J, Candido J, Costa A, Casado P, de Luxán Delgado B, et al. Cancer associated fibroblast FAK regulates malignant cell metabolism. Nat Commun. 2020;11:1290 pubmed publisher
Kretschmer L, Flossdorf M, Mir J, Cho Y, Plambeck M, Treise I, et al. Differential expansion of T central memory precursor and effector subsets is regulated by division speed. Nat Commun. 2020;11:113 pubmed publisher
Brummer G, Fang W, Smart C, Zinda B, Alissa N, Berkland C, et al. CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments. Oncogene. 2020;39:2275-2289 pubmed publisher
Zheng X, Oduro J, Boehme J, Borkner L, Ebensen T, Heise U, et al. Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge. PLoS Pathog. 2019;15:e1008036 pubmed publisher
Burrack A, Spartz E, Raynor J, Wang I, Olson M, Stromnes I. Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma. Cell Rep. 2019;28:2140-2155.e6 pubmed publisher
Niven J, Madelon N, Page N, Caruso A, Harlé G, Lemeille S, et al. Macroautophagy in Dendritic Cells Controls the Homeostasis and Stability of Regulatory T Cells. Cell Rep. 2019;28:21-29.e6 pubmed publisher
Bommareddy P, Lowe D, Kaufman H, Rabkin S, Saha D. Multi-parametric flow cytometry staining procedure for analyzing tumor-infiltrating immune cells following oncolytic herpes simplex virus immunotherapy in intracranial glioblastoma. J Biol Methods. 2019;6: pubmed
Zhang W, Okimura T, Oda T, Jin J. Ascophyllan Induces Activation of Natural Killer Cells in Mice In Vivo and In Vitro. Mar Drugs. 2019;17: pubmed publisher
Kuhn N, Purdon T, van Leeuwen D, Lopez A, Curran K, Daniyan A, et al. CD40 Ligand-Modified Chimeric Antigen Receptor T Cells Enhance Antitumor Function by Eliciting an Endogenous Antitumor Response. Cancer Cell. 2019;35:473-488.e6 pubmed publisher
Farsakoglu Y, Palomino Segura M, Latino I, Zanaga S, Chatziandreou N, Pizzagalli D, et al. Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner. Cell Rep. 2019;26:2307-2315.e5 pubmed publisher
Uchimura T, Oyama Y, Deng M, Guo H, Wilson J, Rampanelli E, et al. The Innate Immune Sensor NLRC3 Acts as a Rheostat that Fine-Tunes T Cell Responses in Infection and Autoimmunity. Immunity. 2018;49:1049-1061.e6 pubmed publisher
Kim C, Lee C, Jung J, Ghosh A, Hasan S, Hwang S, et al. The Transcription Factor Ets1 Suppresses T Follicular Helper Type 2 Cell Differentiation to Halt the Onset of Systemic Lupus Erythematosus. Immunity. 2018;49:1034-1048.e8 pubmed publisher
Chen J, Li N, Yin Y, Zheng N, Min M, Lin B, et al. Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells. Cell Rep. 2018;25:3393-3404.e6 pubmed publisher
Buxade M, Huerga Encabo H, Riera Borrull M, Quintana Gallardo L, López Cotarelo P, Tellechea M, et al. Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5. J Exp Med. 2018;215:2901-2918 pubmed publisher
Moore M, Blachere N, Fak J, Park C, Sawicka K, Parveen S, et al. ZFP36 RNA-binding proteins restrain T cell activation and anti-viral immunity. elife. 2018;7: pubmed publisher
Bhattacharjee P, Keyel P. Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses. Sci Rep. 2018;8:6458 pubmed publisher
Herndler Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, et al. KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity. 2018;48:716-729.e8 pubmed publisher
de Mingo Pulido Á, Gardner A, Hiebler S, Soliman H, Rugo H, Krummel M, et al. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer. Cancer Cell. 2018;33:60-74.e6 pubmed publisher
de Riva A, Wallberg M, Ronchi F, Coulson R, Sage A, Thorne L, et al. Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment. PLoS ONE. 2017;12:e0181964 pubmed publisher
Leonard J, Gilmore D, Dileepan T, Nawrocka W, Chao J, Schoenbach M, et al. Identification of Natural Regulatory T Cell Epitopes Reveals Convergence on a Dominant Autoantigen. Immunity. 2017;47:107-117.e8 pubmed publisher
Lim S, Lee J, Koo J, Kang T, Ha S, Choi J. Cell Type Preference of a Novel Human Derived Cell-Permeable Peptide dNP2 and TAT in Murine Splenic Immune Cells. PLoS ONE. 2016;11:e0155689 pubmed publisher
Uddback I, Pedersen L, Pedersen S, Steffensen M, Holst P, Thomsen A, et al. Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus. Sci Rep. 2016;6:20137 pubmed publisher
Cordero Reyes A, Youker K, Trevino A, Celis R, Hamilton D, Flores Arredondo J, et al. Full Expression of Cardiomyopathy Is Partly Dependent on B-Cells: A Pathway That Involves Cytokine Activation, Immunoglobulin Deposition, and Activation of Apoptosis. J Am Heart Assoc. 2016;5: pubmed publisher
Han J, Chen X, Chu J, Xu B, Meisen W, Chen L, et al. TGFβ Treatment Enhances Glioblastoma Virotherapy by Inhibiting the Innate Immune Response. Cancer Res. 2015;75:5273-82 pubmed publisher
Burrack K, Tan J, McCarthy M, Her Z, Berger J, Ng L, et al. Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells. PLoS Pathog. 2015;11:e1005191 pubmed publisher
Martina M, Noel S, Saxena A, Bandapalle S, Majithia R, Jie C, et al. Double-Negative αβ T Cells Are Early Responders to AKI and Are Found in Human Kidney. J Am Soc Nephrol. 2016;27:1113-23 pubmed publisher
Revelo X, Tsai S, Lei H, Luck H, Ghazarian M, Tsui H, et al. Perforin is a novel immune regulator of obesity-related insulin resistance. Diabetes. 2015;64:90-103 pubmed publisher
Haile S, Horn L, Ostrand Rosenberg S. A soluble form of CD80 enhances antitumor immunity by neutralizing programmed death ligand-1 and simultaneously providing costimulation. Cancer Immunol Res. 2014;2:610-5 pubmed publisher
Ishikawa C, Kawakami H, Uchihara J, Senba M, Mori N. CD69 overexpression by human T-cell leukemia virus type 1 Tax transactivation. Biochim Biophys Acta. 2013;1833:1542-52 pubmed publisher
Tao F, Qian C, Guo W, Luo Q, Xu Q, Sun Y. Inhibition of Th1/Th17 responses via suppression of STAT1 and STAT3 activation contributes to the amelioration of murine experimental colitis by a natural flavonoid glucoside icariin. Biochem Pharmacol. 2013;85:798-807 pubmed publisher
Stanford S, Panchal R, Walker L, Wu D, Falk M, Mitra S, et al. High-throughput screen using a single-cell tyrosine phosphatase assay reveals biologically active inhibitors of tyrosine phosphatase CD45. Proc Natl Acad Sci U S A. 2012;109:13972-7 pubmed publisher
Klezovich Bénard M, Corre J, Jusforgues Saklani H, Fiole D, Burjek N, Tournier J, et al. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathog. 2012;8:e1002481 pubmed publisher
Jordan J, Woods M, Olano J, Walker D. The absence of Toll-like receptor 4 signaling in C3H/HeJ mice predisposes them to overwhelming rickettsial infection and decreased protective Th1 responses. Infect Immun. 2008;76:3717-24 pubmed publisher
Epardaud M, Elpek K, Rubinstein M, Yonekura A, Bellemare Pelletier A, Bronson R, et al. Interleukin-15/interleukin-15R alpha complexes promote destruction of established tumors by reviving tumor-resident CD8+ T cells. Cancer Res. 2008;68:2972-83 pubmed publisher
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
product information
Antigen :
CD69
Apps. Abbrev. :
Flow Cytometry
Cat # :
104506
Clone :
H1.2F3
Item :
FITC anti-mouse CD69
Isotype :
Armenian Hamster IgG
Other Names :
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Size :
500 μg
Price (USD) :
275 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Armenian Hamster
Conjugate/Tag/Label :
FITC
Immunogen :
Mouse dendritic epidermal T cell line Y245
Application Notes :
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation 1-3 , immunohistochemistry 4,5 , and immunoprecipitation 1 . This antibody has been characterized in the literature as containing a lambda (?) light chain.
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA