product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Fire™ 810 anti-mouse/human CD45R/B220
catalog :
103277
quantity :
25 μg
price :
129 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 33
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Dagkonaki A, Papalambrou A, Avloniti M, Gkika A, Evangelidou M, Androutsou M, et al. Maturation of circulating Ly6ChiCCR2+ monocytes by mannan-MOG induces antigen-specific tolerance and reverses autoimmune encephalomyelitis. Front Immunol. 2022;13:972003 pubmed publisher
  • flow cytometry; mouse; loading ...
Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e5c, e7c, 2g, 2i
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s21a, s25a
Satofuka H, Abe S, Moriwaki T, Okada A, Kazuki K, Tanaka H, et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Nat Commun. 2022;13:1841 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 2.5 ug/ml; loading ...; fig 4a
Tatsumi N, Codrington A, El Fenej J, Phondge V, Kumamoto Y. Effective CD4 T cell priming requires repertoire scanning by CD301b+ migratory cDC2 cells upon lymph node entry. Sci Immunol. 2021;6:eabg0336 pubmed publisher
  • flow cytometry; mouse; 1:333; loading ...; fig 2d
Arinrad S, Wilke J, Seelbach A, Doeren J, Hindermann M, Butt U, et al. NMDAR1 autoantibodies amplify behavioral phenotypes of genetic white matter inflammation: a mild encephalitis model with neuropsychiatric relevance. Mol Psychiatry. 2021;: pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3b
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s4a
Wang L, Li X, Hanada Y, Hasuzawa N, Moriyama Y, Nomura M, et al. Dynamin-related protein 1 deficiency accelerates lipopolysaccharide-induced acute liver injury and inflammation in mice. Commun Biol. 2021;4:894 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...
Wilke J, Hindermann M, Moussavi A, Butt U, Dadarwal R, Berghoff S, et al. Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis. Acta Neuropathol Commun. 2021;9:121 pubmed publisher
  • flow cytometry; mouse; fig 2a
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1-1h
Geng G, Liu J, Xu C, Pei Y, Chen L, Mu C, et al. Receptor-mediated mitophagy regulates EPO production and protects against renal anemia. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:200; fig 1j
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2h, 5c
Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
  • flow cytometry; mouse
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Jensen I, Jensen S, Sjaastad F, Gibson Corley K, Dileepan T, Griffith T, et al. Sepsis impedes EAE disease development and diminishes autoantigen-specific naive CD4 T cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5d
Xu A, Barbosa R, Calado D. Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 4f
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Ray S, Chee L, Matson D, Palermo N, Bresnick E, Hewitt K. Sterile α-motif domain requirement for cellular signaling and survival. J Biol Chem. 2020;295:7113-7125 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2s1
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3e
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • flow cytometry; mouse; loading ...
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, 4a
Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Luque Martin R, Van den Bossche J, Furze R, Neele A, van der Velden S, Gijbels M, et al. Targeting Histone Deacetylases in Myeloid Cells Inhibits Their Maturation and Inflammatory Function With Limited Effects on Atherosclerosis. Front Pharmacol. 2019;10:1242 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e6a
Solis A, Bielecki P, Steach H, Sharma L, Harman C, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature. 2019;573:69-74 pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
Tritz Z, Ayasoufi K, Wolf D, Owens C, Malo C, Himes B, et al. Anti-PD-1 and Extended Half-life IL2 Synergize for Treatment of Murine Glioblastoma Independent of Host MHC Class I Expression. Cancer Immunol Res. 2023;11:763-776 pubmed publisher
Deak P, Studnitzer B, Ung T, Steinhardt R, Swartz M, Esser Kahn A. Isolating and targeting a highly active, stochastic dendritic cell subpopulation for improved immune responses. Cell Rep. 2022;41:111563 pubmed publisher
Hobbs B, Matson C, Theofilou V, Webb T, Younis R, Barry E. Deletion Mutants of Francisella Phagosomal Transporters FptA and FptF Are Highly Attenuated for Virulence and Are Protective Against Lethal Intranasal Francisella LVS Challenge in a Murine Model of Respiratory Tularemia. Pathogens. 2021;10: pubmed publisher
Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
product information
Antigen :
CD45R
Apps. Abbrev. :
FC
Cat # :
103277
Clone :
RA3-6B2
Item :
APC/Fire™ 810 anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
25 μg
Price (USD) :
129 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_F810
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation^1, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA