product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Spark NIR™ 685 anti-mouse/human CD45R/B220
catalog :
103267
quantity :
25 μg
price :
165 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 90
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Dagkonaki A, Papalambrou A, Avloniti M, Gkika A, Evangelidou M, Androutsou M, et al. Maturation of circulating Ly6ChiCCR2+ monocytes by mannan-MOG induces antigen-specific tolerance and reverses autoimmune encephalomyelitis. Front Immunol. 2022;13:972003 pubmed publisher
  • flow cytometry; mouse; loading ...
Lee A, Pingali S, Pinilla Ibarz J, Atchison M, Koumenis C, Argon Y, et al. Loss of AID exacerbates the malignant progression of CLL. Leukemia. 2022;36:2430-2442 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e5c, e7c, 2g, 2i
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s21a, s25a
Satofuka H, Abe S, Moriwaki T, Okada A, Kazuki K, Tanaka H, et al. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Nat Commun. 2022;13:1841 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 2.5 ug/ml; loading ...; fig 4a
Tatsumi N, Codrington A, El Fenej J, Phondge V, Kumamoto Y. Effective CD4 T cell priming requires repertoire scanning by CD301b+ migratory cDC2 cells upon lymph node entry. Sci Immunol. 2021;6:eabg0336 pubmed publisher
  • flow cytometry; mouse; 1:333; loading ...; fig 2d
Arinrad S, Wilke J, Seelbach A, Doeren J, Hindermann M, Butt U, et al. NMDAR1 autoantibodies amplify behavioral phenotypes of genetic white matter inflammation: a mild encephalitis model with neuropsychiatric relevance. Mol Psychiatry. 2021;: pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 3b
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s4a
Wang L, Li X, Hanada Y, Hasuzawa N, Moriyama Y, Nomura M, et al. Dynamin-related protein 1 deficiency accelerates lipopolysaccharide-induced acute liver injury and inflammation in mice. Commun Biol. 2021;4:894 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...
Wilke J, Hindermann M, Moussavi A, Butt U, Dadarwal R, Berghoff S, et al. Inducing sterile pyramidal neuronal death in mice to model distinct aspects of gray matter encephalitis. Acta Neuropathol Commun. 2021;9:121 pubmed publisher
  • flow cytometry; mouse; fig 2a
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1-1h
Geng G, Liu J, Xu C, Pei Y, Chen L, Mu C, et al. Receptor-mediated mitophagy regulates EPO production and protects against renal anemia. elife. 2021;10: pubmed publisher
  • flow cytometry; mouse; 1:200; fig 1j
Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2h, 5c
Aslam M, Alemdehy M, Kwesi Maliepaard E, Muhaimin F, Caganova M, Pardieck I, et al. Histone methyltransferase DOT1L controls state-specific identity during B cell differentiation. EMBO Rep. 2021;22:e51184 pubmed publisher
  • flow cytometry; mouse
Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Jensen I, Jensen S, Sjaastad F, Gibson Corley K, Dileepan T, Griffith T, et al. Sepsis impedes EAE disease development and diminishes autoantigen-specific naive CD4 T cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5d
Xu A, Barbosa R, Calado D. Genetic timestamping of plasma cells in vivo reveals tissue-specific homeostatic population turnover. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 4f
Cao W, Fang F, Gould T, Li X, Kim C, Gustafson C, et al. Ecto-NTPDase CD39 is a negative checkpoint that inhibits follicular helper cell generation. J Clin Invest. 2020;130:3422-3436 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Ray S, Chee L, Matson D, Palermo N, Bresnick E, Hewitt K. Sterile α-motif domain requirement for cellular signaling and survival. J Biol Chem. 2020;295:7113-7125 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 2s1
Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Fu Y, Ding Y, Wang Q, Zhu F, Tan Y, Lu X, et al. Blood-stage malaria parasites manipulate host innate immune responses through the induction of sFGL2. Sci Adv. 2020;6:eaay9269 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3e
Luker A, Graham L, Smith T, Camarena C, Zellner M, Gilmer J, et al. The DNA methyltransferase inhibitor, guadecitabine, targets tumor-induced myelopoiesis and recovers T cell activity to slow tumor growth in combination with adoptive immunotherapy in a mouse model of breast cancer. BMC Immunol. 2020;21:8 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3e
Zhang Q, Xiang L, Zaman M, Dong W, He G, Deng G. Predominant Role of Immunoglobulin G in the Pathogenesis of Splenomegaly in Murine Lupus. Front Immunol. 2019;10:3020 pubmed publisher
  • flow cytometry; mouse; loading ...
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2d
Uckelmann H, Kim S, Wong E, Hatton C, Giovinazzo H, Gadrey J, et al. Therapeutic targeting of preleukemia cells in a mouse model of NPM1 mutant acute myeloid leukemia. Science. 2020;367:586-590 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s16a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1
Mesin L, Schiepers A, Ersching J, Barbulescu A, Cavazzoni C, Angelini A, et al. Restricted Clonality and Limited Germinal Center Reentry Characterize Memory B Cell Reactivation by Boosting. Cell. 2020;180:92-106.e11 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, 4a
Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Luque Martin R, Van den Bossche J, Furze R, Neele A, van der Velden S, Gijbels M, et al. Targeting Histone Deacetylases in Myeloid Cells Inhibits Their Maturation and Inflammatory Function With Limited Effects on Atherosclerosis. Front Pharmacol. 2019;10:1242 pubmed publisher
  • flow cytometry; mouse; loading ...; fig e6a
Solis A, Bielecki P, Steach H, Sharma L, Harman C, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature. 2019;573:69-74 pubmed publisher
  • flow cytometry; mouse; loading ...
Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Takagaki S, Yamashita R, Hashimoto N, Sugihara K, Kanari K, Tabata K, et al. Galactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow. Sci Rep. 2019;9:7133 pubmed publisher
Tritz Z, Ayasoufi K, Wolf D, Owens C, Malo C, Himes B, et al. Anti-PD-1 and Extended Half-life IL2 Synergize for Treatment of Murine Glioblastoma Independent of Host MHC Class I Expression. Cancer Immunol Res. 2023;11:763-776 pubmed publisher
Goddery E, Fain C, Lipovsky C, Ayasoufi K, Yokanovich L, Malo C, et al. Microglia and Perivascular Macrophages Act as Antigen Presenting Cells to Promote CD8 T Cell Infiltration of the Brain. Front Immunol. 2021;12:726421 pubmed publisher
Fang H, Bonora G, Lewandowski J, Thakur J, Filippova G, Henikoff S, et al. Trans- and cis-acting effects of Firre on epigenetic features of the inactive X chromosome. Nat Commun. 2020;11:6053 pubmed publisher
Reed J, Preston Hurlburt P, Philbrick W, Betancur G, Korah M, LUCAS C, et al. The receptor for advanced glycation endproducts (RAGE) modulates T cell signaling. PLoS ONE. 2020;15:e0236921 pubmed publisher
Lechuga Vieco A, Latorre Pellicer A, Johnston I, Prota G, Gileadi U, Justo Méndez R, et al. Cell identity and nucleo-mitochondrial genetic context modulate OXPHOS performance and determine somatic heteroplasmy dynamics. Sci Adv. 2020;6:eaba5345 pubmed publisher
Jeon I, Lee J, Shin K, Kang T, Park M, Seo H, et al. Enhanced Immunogenicity of Engineered HER2 Antigens Potentiates Antitumor Immune Responses. Vaccines (Basel). 2020;8: pubmed publisher
Zhao R, Zhang H, Zhang Y, Li D, Huang C, Li F. In vivo Screen Identifies Zdhhc2 as a Critical Regulator of Germinal Center B Cell Differentiation. Front Immunol. 2020;11:1025 pubmed publisher
Woodruff M, Ramonell R, Cashman K, Nguyen D, Ley A, Kyu S, et al. Critically ill SARS-CoV-2 patients display lupus-like hallmarks of extrafollicular B cell activation. medRxiv. 2020;: pubmed publisher
Bekeschus S, Clemen R, Nießner F, Sagwal S, Freund E, Schmidt A. Medical Gas Plasma Jet Technology Targets Murine Melanoma in an Immunogenic Fashion. Adv Sci (Weinh). 2020;7:1903438 pubmed publisher
Yamamoto K, Venida A, Yano J, Biancur D, Kakiuchi M, Gupta S, et al. Autophagy promotes immune evasion of pancreatic cancer by degrading MHC-I. Nature. 2020;581:100-105 pubmed publisher
Wu Y, Chou T, Young L, Hsieh F, Pan H, Mo S, et al. Tumor suppressor death-associated protein kinase 1 inhibits necroptosis by p38 MAPK activation. Cell Death Dis. 2020;11:305 pubmed publisher
Zang M, Guo J, Liu L, Jin F, Feng X, An G, et al. Cdc37 suppression induces plasma cell immaturation and bortezomib resistance in multiple myeloma via Xbp1s. Oncogenesis. 2020;9:31 pubmed publisher
Ramalingam P, Poulos M, Lazzari E, Gutkin M, Lopez D, Kloss C, et al. Chronic activation of endothelial MAPK disrupts hematopoiesis via NFKB dependent inflammatory stress reversible by SCGF. Nat Commun. 2020;11:666 pubmed publisher
Chen C, Yu W, Tober J, Gao P, He B, Lee K, et al. Spatial Genome Re-organization between Fetal and Adult Hematopoietic Stem Cells. Cell Rep. 2019;29:4200-4211.e7 pubmed publisher
Yan J, Allen S, McDonald E, Das I, Mak J, Liu L, et al. MAIT Cells Promote Tumor Initiation, Growth, and Metastases via Tumor MR1. Cancer Discov. 2019;: pubmed publisher
Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
Frodermann V, Rohde D, Courties G, Severe N, Schloss M, Amatullah H, et al. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells. Nat Med. 2019;25:1761-1771 pubmed publisher
Khanom U, Ohigashi I, Fujimori S, Kondo K, Takada K, Takahama Y. TCR Affinity for In Vivo Peptide-Induced Thymic Positive Selection Fine-Tunes TCR Responsiveness of Peripheral CD8+ T Cells. J Immunol. 2019;: pubmed publisher
Darrah E, Jondle C, Johnson K, Xin G, Lange P, Cui W, et al. Conserved Gammaherpesvirus Protein Kinase Selectively Promotes Irrelevant B Cell Responses. J Virol. 2019;93: pubmed publisher
Bussey K, Murthy S, Reimer E, Chan B, Hatesuer B, Schughart K, et al. Endosomal Toll-Like Receptors 7 and 9 Cooperate in Detection of Murine Gammaherpesvirus 68 Infection. J Virol. 2019;93: pubmed publisher
Oldstone M, Ware B, Horton L, Welch M, Aiolfi R, Zarpellon A, et al. Lymphocytic choriomeningitis virus Clone 13 infection causes either persistence or acute death dependent on IFN-1, cytotoxic T lymphocytes (CTLs), and host genetics. Proc Natl Acad Sci U S A. 2018;115:E7814-E7823 pubmed publisher
Petvises S, Periasamy P, O Neill H. MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis. BMC Immunol. 2018;19:21 pubmed publisher
Robak O, Heimesaat M, Kruglov A, Prepens S, Ninnemann J, Gutbier B, et al. Antibiotic treatment-induced secondary IgA deficiency enhances susceptibility to Pseudomonas aeruginosa pneumonia. J Clin Invest. 2018;128:3535-3545 pubmed publisher
Valentine K, Davini D, Lawrence T, Mullins G, Manansala M, Al Kuhlani M, et al. CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease. J Immunol. 2018;201:31-40 pubmed publisher
Kuo P, Jatiani S, Rahman A, Edwards D, Jiang Z, Ahr K, et al. SOX11 augments BCR signaling to drive MCL-like tumor development. Blood. 2018;131:2247-2255 pubmed publisher
Sui P, Wiesner D, Xu J, Zhang Y, Lee J, Van Dyken S, et al. Pulmonary neuroendocrine cells amplify allergic asthma responses. Science. 2018;360: pubmed publisher
Jesteadt E, Zhang I, Yu H, Meierovics A, Chua Yankelevich W, Cowley S. Interleukin-18 Is Critical for Mucosa-Associated Invariant T Cell Gamma Interferon Responses to Francisella Species In Vitro but Not In Vivo. Infect Immun. 2018;86: pubmed publisher
King E, Mazor R, Cuburu N, Pastan I. Low-Dose Methotrexate Prevents Primary and Secondary Humoral Immune Responses and Induces Immune Tolerance to a Recombinant Immunotoxin. J Immunol. 2018;200:2038-2045 pubmed publisher
Pitts M, Combs T, D Orazio S. Neutrophils from Both Susceptible and Resistant Mice Efficiently Kill Opsonized Listeria monocytogenes. Infect Immun. 2018;86: pubmed publisher
Meier L, Auger J, Engelson B, Cowan H, Breed E, Gonzalez Torres M, et al. CD301b/MGL2+ Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and Fibrosis. Circulation. 2018;137:2478-2493 pubmed publisher
Qing X, Chinenov Y, Redecha P, Madaio M, Roelofs J, FARBER G, et al. iRhom2 promotes lupus nephritis through TNF-? and EGFR signaling. J Clin Invest. 2018;128:1397-1412 pubmed publisher
Garaycoechea J, Crossan G, Langevin F, Mulderrig L, Louzada S, Yang F, et al. Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018;553:171-177 pubmed publisher
Muro R, Nitta T, Nakano K, Okamura T, Takayanagi H, Suzuki H. γδTCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program. J Clin Invest. 2018;128:415-426 pubmed publisher
Singh P, Hoggatt J, Kamocka M, Mohammad K, Saunders M, Li H, et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest. 2017;127:4527-4540 pubmed publisher
Ando T, Kashiwakura J, Itoh Nagato N, Yamashita H, Baba M, Kawakami Y, et al. Histamine-releasing factor enhances food allergy. J Clin Invest. 2017;127:4541-4553 pubmed publisher
Albertí Servera L, von Muenchow L, Tsapogas P, Capoferri G, Eschbach K, Beisel C, et al. Single-cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors. EMBO J. 2017;36:3619-3633 pubmed publisher
Jain R, Sheridan J, Policheni A, Heinlein M, Gandolfo L, Dewson G, et al. A critical epithelial survival axis regulated by MCL-1 maintains thymic function in mice. Blood. 2017;130:2504-2515 pubmed publisher
El Zaatari M, Bass A, Bowlby R, Zhang M, Syu L, Yang Y, et al. Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity. Gastroenterology. 2018;154:140-153.e17 pubmed publisher
Imperatore F, Maurizio J, Vargas Aguilar S, Busch C, Favret J, Kowenz Leutz E, et al. SIRT1 regulates macrophage self-renewal. EMBO J. 2017;36:2353-2372 pubmed publisher
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Wu S, Niknafs Y, Kim S, Oravecz Wilson K, Zajac C, Toubai T, et al. A Critical Analysis of the Role of SNARE Protein SEC22B in Antigen Cross-Presentation. Cell Rep. 2017;19:2645-2656 pubmed publisher
Stewart Hutchinson P, Szasz T, Jaeger E, Onken M, Cooper J, Morley S. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate. J Leukoc Biol. 2017;102:941-948 pubmed publisher
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Probst P, Kopp J, Oxenius A, Colombo M, Ritz D, Fugmann T, et al. Sarcoma Eradication by Doxorubicin and Targeted TNF Relies upon CD8+ T-cell Recognition of a Retroviral Antigen. Cancer Res. 2017;77:3644-3654 pubmed publisher
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Mukai K, Karasuyama H, Kabashima K, Kubo M, Galli S. Differences in the Importance of Mast Cells, Basophils, IgE, and IgG versus That of CD4+ T Cells and ILC2 Cells in Primary and Secondary Immunity to Strongyloides venezuelensis. Infect Immun. 2017;85: pubmed publisher
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Nassar M, Tabib Y, Capucha T, Mizraji G, Nir T, Pevsner Fischer M, et al. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa. Proc Natl Acad Sci U S A. 2017;114:E337-E346 pubmed publisher
product information
Antigen :
CD45R
Apps. Abbrev. :
FC
Cat # :
103267
Clone :
RA3-6B2
Item :
Spark NIR™ 685 anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
25 μg
Price (USD) :
165 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
SPKNIR_685
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation^1, in vitro and in vivo modulation of B cell responses^2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^5,6, and spatial biology (IBEX)^14,15.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA