product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220
catalog :
103235
quantity :
25 μg
price :
83 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP/Cy5.5
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 76
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:300; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5b
Barker K, Etesami N, Shenoy A, Arafa E, Lyon de Ana C, Smith N, et al. Lung-resident memory B cells protect against bacterial pneumonia. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Sugita J, Fujiu K, Nakayama Y, Matsubara T, Matsuda J, Oshima T, et al. Cardiac macrophages prevent sudden death during heart stress. Nat Commun. 2021;12:1910 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Shishido T, Kohyama M, Nakai W, Matsumoto M, Miyata H, Suenaga T, et al. Invariant chain p41 mediates production of soluble MHC class II molecules. Biochem Biophys Res Commun. 2018;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Thomson C, van de Pavert S, Stakenborg M, Labeeuw E, Matteoli G, Mowat A, et al. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators. J Immunol. 2018;201:215-229 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Garaycoechea J, Crossan G, Langevin F, Mulderrig L, Louzada S, Yang F, et al. Alcohol and endogenous aldehydes damage chromosomes and mutate stem cells. Nature. 2018;553:171-177 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Degn S, van der Poel C, Firl D, Ayoglu B, Al Qureshah F, Bajic G, et al. Clonal Evolution of Autoreactive Germinal Centers. Cell. 2017;170:913-926.e19 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Jinnohara T, Kanaya T, Hase K, Sakakibara S, Kato T, Tachibana N, et al. IL-22BP dictates characteristics of Peyer's patch follicle-associated epithelium for antigen uptake. J Exp Med. 2017;214:1607-1618 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1i
Forster M, Farrington K, Petrov J, Belle J, Mindt B, Witalis M, et al. MYSM1-dependent checkpoints in B cell lineage differentiation and B cell-mediated immune response. J Leukoc Biol. 2017;101:643-654 pubmed publisher
  • flow cytometry; mouse; tbl s3
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Vijay R, Guthmiller J, Sturtz A, Crooks S, Johnson J, Li L, et al. Hemolysis-associated phosphatidylserine exposure promotes polyclonal plasmablast differentiation. J Exp Med. 2021;218: pubmed publisher
Gern B, Adams K, Plumlee C, Stoltzfus C, Shehata L, Moguche A, et al. TGFβ restricts expansion, survival, and function of T cells within the tuberculous granuloma. Cell Host Microbe. 2021;29:594-606.e6 pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
Mincham K, Jones A, Bodinier M, Scott N, Lauzon Joset J, Stumbles P, et al. Transplacental Innate Immune Training via Maternal Microbial Exposure: Role of XBP1-ERN1 Axis in Dendritic Cell Precursor Programming. Front Immunol. 2020;11:601494 pubmed publisher
Qi T, Sun M, Zhang C, Chen P, Xiao C, Chang X. Ascorbic Acid Promotes Plasma Cell Differentiation through Enhancing TET2/3-Mediated DNA Demethylation. Cell Rep. 2020;33:108452 pubmed publisher
Simoni L, Presumey J, van der Poel C, Castrillón C, Chang S, Utz P, et al. Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020;33:108330 pubmed publisher
Montel Hagen A, Sun V, Casero D, Tsai S, Zampieri A, Jackson N, et al. In Vitro Recapitulation of Murine Thymopoiesis from Single Hematopoietic Stem Cells. Cell Rep. 2020;33:108320 pubmed publisher
Baker G, Muhlich J, Palaniappan S, Moore J, Davis S, Santagata S, et al. SYLARAS: A Platform for the Statistical Analysis and Visual Display of Systemic Immunoprofiling Data and Its Application to Glioblastoma. Cell Syst. 2020;11:272-285.e9 pubmed publisher
Li Y, Kong W, Yang W, Patel R, Casey E, Okeyo Owuor T, et al. Single-Cell Analysis of Neonatal HSC Ontogeny Reveals Gradual and Uncoordinated Transcriptional Reprogramming that Begins before Birth. Cell Stem Cell. 2020;27:732-747.e7 pubmed publisher
Yang J, Wang W, Chen Z, Lu S, Yang F, Bi Z, et al. A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity. Nature. 2020;586:572-577 pubmed publisher
Vijay R, Guthmiller J, Sturtz A, Surette F, Rogers K, Sompallae R, et al. Infection-induced plasmablasts are a nutrient sink that impairs humoral immunity to malaria. Nat Immunol. 2020;21:790-801 pubmed publisher
Wang X, Luo X, Chen C, Tang Y, Li L, Mo B, et al. The Ap-2α/Elk-1 axis regulates Sirpα-dependent tumor phagocytosis by tumor-associated macrophages in colorectal cancer. Signal Transduct Target Ther. 2020;5:35 pubmed publisher
Lang J, Bohn P, Bhat H, Jastrow H, Walkenfort B, Cansiz F, et al. Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. Nat Commun. 2020;11:1338 pubmed publisher
Kunishita Y, Yoshimi R, Kamiyama R, Kishimoto D, Yoshida K, Hashimoto E, et al. TRIM21 Dysfunction Enhances Aberrant B-Cell Differentiation in Autoimmune Pathogenesis. Front Immunol. 2020;11:98 pubmed publisher
Li K, Liu Y, Cao H, Zhang Y, Gu Z, Liu X, et al. Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing. Nat Commun. 2020;11:485 pubmed publisher
Chen L, Christian D, Kochanowsky J, Phan A, Clark J, Wang S, et al. The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses. J Exp Med. 2020;217: pubmed publisher
Fachi J, Sécca C, Rodrigues P, Mato F, Di Luccia B, Felipe J, et al. Acetate coordinates neutrophil and ILC3 responses against C. difficile through FFAR2. J Exp Med. 2020;217: pubmed publisher
Elsner R, Shlomchik M. IL-12 Blocks Tfh Cell Differentiation during Salmonella Infection, thereby Contributing to Germinal Center Suppression. Cell Rep. 2019;29:2796-2809.e5 pubmed publisher
van der Poel C, Bajic G, Macaulay C, van den Broek T, Ellson C, Bouma G, et al. Follicular Dendritic Cells Modulate Germinal Center B Cell Diversity through FcγRIIB. Cell Rep. 2019;29:2745-2755.e4 pubmed publisher
Hilpert C, Sitte S, Arnold H, Lehmann C, Dudziak D, Mattner J, et al. Dendritic Cells Control Regulatory T Cell Function Required for Maintenance of Intestinal Tissue Homeostasis. J Immunol. 2019;203:3068-3077 pubmed publisher
Olson W, Jakic B, Labi V, Schoeler K, Kind M, Klepsch V, et al. Orphan Nuclear Receptor NR2F6 Suppresses T Follicular Helper Cell Accumulation through Regulation of IL-21. Cell Rep. 2019;28:2878-2891.e5 pubmed publisher
Lutzmann M, Bernex F, Da Costa De Jesus C, Hodroj D, Marty C, Plo I, et al. MCM8- and MCM9 Deficiencies Cause Lifelong Increased Hematopoietic DNA Damage Driving p53-Dependent Myeloid Tumors. Cell Rep. 2019;28:2851-2865.e4 pubmed publisher
Rivadeneira D, DePeaux K, Wang Y, Kulkarni A, Tabib T, Menk A, et al. Oncolytic Viruses Engineered to Enforce Leptin Expression Reprogram Tumor-Infiltrating T Cell Metabolism and Promote Tumor Clearance. Immunity. 2019;51:548-560.e4 pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Schoeler K, Aufschnaiter A, Messner S, Derudder E, Herzog S, Villunger A, et al. TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells. FEBS J. 2019;286:3566-3581 pubmed publisher
Leal A, Misek S, Lisabeth E, Neubig R, Liby K. The Rho/MRTF pathway inhibitor CCG-222740 reduces stellate cell activation and modulates immune cell populations in KrasG12D; Pdx1-Cre (KC) mice. Sci Rep. 2019;9:7072 pubmed publisher
Paris J, Morgan M, Campos J, Spencer G, Shmakova A, Ivanova I, et al. Targeting the RNA m6A Reader YTHDF2 Selectively Compromises Cancer Stem Cells in Acute Myeloid Leukemia. Cell Stem Cell. 2019;: pubmed publisher
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
Tan D, Li Y, Yang C, Li J, Tan S, Chin D, et al. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells. Cell Rep. 2019;26:2316-2328.e6 pubmed publisher
Gorman J, Hundhausen C, Kinsman M, Arkatkar T, Allenspach E, Clough C, et al. The TYK2-P1104A Autoimmune Protective Variant Limits Coordinate Signals Required to Generate Specialized T Cell Subsets. Front Immunol. 2019;10:44 pubmed publisher
Stivala S, Codilupi T, Brkic S, Baerenwaldt A, Ghosh N, Hao Shen H, et al. Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms. J Clin Invest. 2019;130:1596-1611 pubmed publisher
Kobayashi T, Voisin B, Kim D, Kennedy E, Jo J, Shih H, et al. Homeostatic Control of Sebaceous Glands by Innate Lymphoid Cells Regulates Commensal Bacteria Equilibrium. Cell. 2019;176:982-997.e16 pubmed publisher
Nakamura Ishizu A, Matsumura T, Stumpf P, Umemoto T, Takizawa H, Takihara Y, et al. Thrombopoietin Metabolically Primes Hematopoietic Stem Cells to Megakaryocyte-Lineage Differentiation. Cell Rep. 2018;25:1772-1785.e6 pubmed publisher
Di Liberto G, Pantelyushin S, Kreutzfeldt M, Page N, Musardo S, Coras R, et al. Neurons under T Cell Attack Coordinate Phagocyte-Mediated Synaptic Stripping. Cell. 2018;175:458-471.e19 pubmed publisher
Firl D, Degn S, PADERA T, Carroll M. Capturing change in clonal composition amongst single mouse germinal centers. elife. 2018;7: pubmed publisher
Morales Hernández A, Martinat A, Chabot A, Kang G, McKinney Freeman S. Elevated Oxidative Stress Impairs Hematopoietic Progenitor Function in C57BL/6 Substrains. Stem Cell Reports. 2018;11:334-347 pubmed publisher
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Page N, Klimek B, De Roo M, Steinbach K, Soldati H, Lemeille S, et al. Expression of the DNA-Binding Factor TOX Promotes the Encephalitogenic Potential of Microbe-Induced Autoreactive CD8+ T Cells. Immunity. 2018;48:937-950.e8 pubmed publisher
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Beloor J, Maes N, Ullah I, Uchil P, Jackson A, Fikrig E, et al. Small Interfering RNA-Mediated Control of Virus Replication in the CNS Is Therapeutic and Enables Natural Immunity to West Nile Virus. Cell Host Microbe. 2018;23:549-556.e3 pubmed publisher
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Yasuoka T, Kuwahara M, Yamada T, Maruyama S, Suzuki J, Taniguchi M, et al. The Transcriptional Repressor Gfi1 Plays a Critical Role in the Development of NKT1- and NKT2-Type iNKT Cells. PLoS ONE. 2016;11:e0157395 pubmed publisher
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Belle J, Petrov J, Langlais D, Robert F, Cencic R, Shen S, et al. Repression of p53-target gene Bbc3/PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance. Cell Death Differ. 2016;23:759-75 pubmed publisher
Kong S, Dong H, Song J, Thiruppathi M, Prabhakar B, Qiu Q, et al. Deleted in Breast Cancer 1 Suppresses B Cell Activation through RelB and Is Regulated by IKKα Phosphorylation. J Immunol. 2015;195:3685-93 pubmed publisher
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product information
Antigen :
CD45R
Apps. Abbrev. :
Flow Cytometry
Cat # :
103235
Clone :
RA3-6B2
Item :
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
25 μg
Price (USD) :
83 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cat (Feline)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP_CYANINE5.5
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 , and spatial biology (IBEX) 14,15 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA