product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse/human CD45R/B220
catalog :
103227
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 71
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:1000; fig s6g
Lin J, Liu H, Fukumoto T, Zundell J, Yan Q, Tang C, et al. Targeting the IRE1α/XBP1s pathway suppresses CARM1-expressing ovarian cancer. Nat Commun. 2021;12:5321 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4a
Luque Martin R, Van den Bossche J, Furze R, Neele A, van der Velden S, Gijbels M, et al. Targeting Histone Deacetylases in Myeloid Cells Inhibits Their Maturation and Inflammatory Function With Limited Effects on Atherosclerosis. Front Pharmacol. 2019;10:1242 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1d
Chopin M, Lun A, Zhan Y, Schreuder J, Coughlan H, D Amico A, et al. Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and Function via Induction of Transcriptional Regulator DC-SCRIPT. Immunity. 2019;50:77-90.e5 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4g
Lin Y, Pecetta S, Steichen J, Kratochvil S, Melzi E, Arnold J, et al. One-step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock-in mice. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig s4a
Gaya M, Barral P, Burbage M, Aggarwal S, Montaner B, Warren Navia A, et al. Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells. Cell. 2018;172:517-533.e20 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1d
Kumar B, Garcia M, Weng L, Jung X, Murakami J, Hu X, et al. Acute myeloid leukemia transforms the bone marrow niche into a leukemia-permissive microenvironment through exosome secretion. Leukemia. 2018;32:575-587 pubmed publisher
  • flow cytometry; mouse; loading ...
Rychtarčíková Z, Lettlova S, Tomkova V, Korenkova V, Langerova L, Simonova E, et al. Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism. Oncotarget. 2017;8:6376-6398 pubmed publisher
  • flow cytometry; mouse; loading ...
Cecchinato V, Bernasconi E, Speck R, Proietti M, Sauermann U, D Agostino G, et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J Immunol. 2017;198:184-195 pubmed
  • flow cytometry; mouse; loading ...; fig 8a
Griffiths K, Dolezal O, Cao B, Nilsson S, See H, Pfleger K, et al. i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. J Biol Chem. 2016;291:12641-57 pubmed publisher
  • flow cytometry; mouse; fig 2
Wong E, Soni C, Chan A, Domeier P, Shwetank -, Abraham T, et al. B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance. J Immunol. 2015;194:4130-43 pubmed publisher
Jeljeli M, Chêne C, Chouzenoux S, Thomas M, Segain B, Doridot L, et al. LPSlow-Macrophages Alleviate the Outcome of Graft-Versus-Host Disease Without Aggravating Lymphoma Growth in Mice. Front Immunol. 2021;12:670776 pubmed publisher
Schäfer A, Eichhorst A, Hentze C, Kraemer A, Amend A, Sprenger D, et al. Low Dietary Fiber Intake Links Development of Obesity and Lupus Pathogenesis. Front Immunol. 2021;12:696810 pubmed publisher
Chen C, Chen B, Wang Y, Curman P, Beilinson H, Brecht R, et al. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity is required for V(D)J recombination. J Exp Med. 2021;218: pubmed publisher
Lazarian G, Yin S, ten Hacken E, Sewastianik T, Uduman M, Font Tello A, et al. A hotspot mutation in transcription factor IKZF3 drives B cell neoplasia via transcriptional dysregulation. Cancer Cell. 2021;39:380-393.e8 pubmed publisher
Wang X, Ray R, Kratochvil S, Melzi E, Lin Y, Giguere S, et al. Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptors. EMBO J. 2021;40:e105926 pubmed publisher
Alexandre Y, Devi S, Park S, Mackay L, Heath W, Mueller S. Systemic Inflammation Suppresses Lymphoid Tissue Remodeling and B Cell Immunity during Concomitant Local Infection. Cell Rep. 2020;33:108567 pubmed publisher
Simoni L, Presumey J, van der Poel C, Castrillón C, Chang S, Utz P, et al. Complement C4A Regulates Autoreactive B Cells in Murine Lupus. Cell Rep. 2020;33:108330 pubmed publisher
Kobia F, Preusse K, Dai Q, Weaver N, Hass M, Chaturvedi P, et al. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol. 2020;18:e3000850 pubmed publisher
Gajdasik D, Gaspal F, Halford E, Fiancette R, Dutton E, Willis C, et al. Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues. Nat Commun. 2020;11:3421 pubmed publisher
Bellomo A, Mondor I, Spinelli L, Lagueyrie M, Stewart B, Brouilly N, et al. Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages. Immunity. 2020;53:127-142.e7 pubmed publisher
Perry J, Tian S, Sengottuvel N, Harrison E, Gorentla B, Kapadia C, et al. Pulmonary Delivery of Nanoparticle-Bound Toll-like Receptor 9 Agonist for the Treatment of Metastatic Lung Cancer. ACS Nano. 2020;14:7200-7215 pubmed publisher
Soni C, Perez O, Voss W, Pucella J, Serpas L, Mehl J, et al. Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA. Immunity. 2020;52:1022-1038.e7 pubmed publisher
Raju S, Verbaro D, Egawa T. PD-1 Signaling Promotes Control of Chronic Viral Infection by Restricting Type-I-Interferon-Mediated Tissue Damage. Cell Rep. 2019;29:2556-2564.e3 pubmed publisher
Burgener S, Leborgne N, Snipas S, Salvesen G, Bird P, Benarafa C. Cathepsin G Inhibition by Serpinb1 and Serpinb6 Prevents Programmed Necrosis in Neutrophils and Monocytes and Reduces GSDMD-Driven Inflammation. Cell Rep. 2019;27:3646-3656.e5 pubmed publisher
Cantor D, King B, Blumenberg L, Dimauro T, Aifantis I, Koralov S, et al. Impaired Expression of Rearranged Immunoglobulin Genes and Premature p53 Activation Block B Cell Development in BMI1 Null Mice. Cell Rep. 2019;26:108-118.e4 pubmed publisher
Benhamou D, Labi V, Getahun A, Benchetrit E, Dowery R, Rajewsky K, et al. The c-Myc/miR17-92/PTEN Axis Tunes PI3K Activity to Control Expression of Recombination Activating Genes in Early B Cell Development. Front Immunol. 2018;9:2715 pubmed publisher
Teo T, Lum F, Ghaffar K, Chan Y, Amrun S, Tan J, et al. Plasmodium co-infection protects against chikungunya virus-induced pathologies. Nat Commun. 2018;9:3905 pubmed publisher
Hung P, Johnson B, Chen B, Byrum A, Bredemeyer A, Yewdell W, et al. MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining. Mol Cell. 2018;71:332-342.e8 pubmed publisher
Abbott R, Lee J, Menis S, Skog P, Rossi M, Ota T, et al. Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens. Immunity. 2018;48:133-146.e6 pubmed publisher
Tanaka Y, Sotome T, Inoue A, Mukozu T, Kuwabara T, Mikami T, et al. SATB1 Conditional Knockout Results in Sjögren's Syndrome in Mice. J Immunol. 2017;199:4016-4022 pubmed publisher
Hamilton J, Wu Q, Yang P, Luo B, Liu S, Hong H, et al. Cutting Edge: Endogenous IFN-β Regulates Survival and Development of Transitional B Cells. J Immunol. 2017;199:2618-2623 pubmed publisher
Kälin S, Becker M, Ott V, Serr I, Hosp F, Mollah M, et al. A Stat6/Pten Axis Links Regulatory T Cells with Adipose Tissue Function. Cell Metab. 2017;26:475-492.e7 pubmed publisher
Nair S, Poddar S, Shimak R, Diamond M. Interferon Regulatory Factor 1 Protects against Chikungunya Virus-Induced Immunopathology by Restricting Infection in Muscle Cells. J Virol. 2017;91: pubmed publisher
Courtney A, Amacher J, Kadlecek T, Mollenauer M, Au Yeung B, Kuriyan J, et al. A Phosphosite within the SH2 Domain of Lck Regulates Its Activation by CD45. Mol Cell. 2017;67:498-511.e6 pubmed publisher
Carbonetti S, Oliver B, Vigdorovich V, Dambrauskas N, Sack B, Bergl E, et al. A method for the isolation and characterization of functional murine monoclonal antibodies by single B cell cloning. J Immunol Methods. 2017;448:66-73 pubmed publisher
Ilsley M, Gillinder K, Magor G, Huang S, Bailey T, Crossley M, et al. Krüppel-like factors compete for promoters and enhancers to fine-tune transcription. Nucleic Acids Res. 2017;45:6572-6588 pubmed publisher
Sato K, Kato A, Sekai M, Hamazaki Y, Minato N. Physiologic Thymic Involution Underlies Age-Dependent Accumulation of Senescence-Associated CD4+ T Cells. J Immunol. 2017;199:138-148 pubmed publisher
Murphy M, Pattabiraman G, Manavalan T, Medvedev A. Deficiency in IRAK4 activity attenuates manifestations of murine Lupus. Eur J Immunol. 2017;47:880-891 pubmed publisher
Hor J, Heath W, Mueller S. Neutrophils are dispensable in the modulation of T cell immunity against cutaneous HSV-1 infection. Sci Rep. 2017;7:41091 pubmed publisher
Jaitin D, Weiner A, Yofe I, Lara Astiaso D, Keren Shaul H, David E, et al. Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. Cell. 2016;167:1883-1896.e15 pubmed publisher
Sebina I, James K, Soon M, Fogg L, Best S, Labastida Rivera F, et al. IFNAR1-Signalling Obstructs ICOS-mediated Humoral Immunity during Non-lethal Blood-Stage Plasmodium Infection. PLoS Pathog. 2016;12:e1005999 pubmed publisher
Miyauchi K, Sugimoto Ishige A, Harada Y, Adachi Y, Usami Y, Kaji T, et al. Protective neutralizing influenza antibody response in the absence of T follicular helper cells. Nat Immunol. 2016;17:1447-1458 pubmed publisher
Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher
Georgoudaki A, Prokopec K, Boura V, Hellqvist E, Sohn S, Östling J, et al. Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis. Cell Rep. 2016;15:2000-11 pubmed publisher
Domeier P, Chodisetti S, Soni C, Schell S, Elias M, Wong E, et al. IFN-γ receptor and STAT1 signaling in B cells are central to spontaneous germinal center formation and autoimmunity. J Exp Med. 2016;213:715-32 pubmed publisher
Onodera T, Hosono A, Odagiri T, Tashiro M, Kaminogawa S, Okuno Y, et al. Whole-Virion Influenza Vaccine Recalls an Early Burst of High-Affinity Memory B Cell Response through TLR Signaling. J Immunol. 2016;196:4172-84 pubmed publisher
Serr I, Fürst R, Achenbach P, Scherm M, Gökmen F, Haupt F, et al. Type 1 diabetes vaccine candidates promote human Foxp3(+)Treg induction in humanized mice. Nat Commun. 2016;7:10991 pubmed publisher
Baglaenko Y, Manion K, Chang N, Gracey E, Loh C, Wither J. IL-10 Production Is Critical for Sustaining the Expansion of CD5+ B and NKT Cells and Restraining Autoantibody Production in Congenic Lupus-Prone Mice. PLoS ONE. 2016;11:e0150515 pubmed publisher
Uddback I, Pedersen L, Pedersen S, Steffensen M, Holst P, Thomsen A, et al. Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus. Sci Rep. 2016;6:20137 pubmed publisher
Yoshikawa S, Usami T, Kikuta J, Ishii M, Sasano T, Sugiyama K, et al. Intravital imaging of Ca(2+) signals in lymphocytes of Ca(2+) biosensor transgenic mice: indication of autoimmune diseases before the pathological onset. Sci Rep. 2016;6:18738 pubmed publisher
Adachi Y, Onodera T, Yamada Y, Daio R, Tsuiji M, Inoue T, et al. Distinct germinal center selection at local sites shapes memory B cell response to viral escape. J Exp Med. 2015;212:1709-23 pubmed publisher
Minamitani T, Yasui T, Ma Y, Zhou H, Okuzaki D, Tsai C, et al. Evasion of affinity-based selection in germinal centers by Epstein-Barr virus LMP2A. Proc Natl Acad Sci U S A. 2015;112:11612-7 pubmed publisher
Grosjean F, Nasi S, Schneider P, Chobaz V, Liu A, Mordasini V, et al. Dendritic Cells Cause Bone Lesions in a New Mouse Model of Histiocytosis. PLoS ONE. 2015;10:e0133917 pubmed publisher
Kato Y, Zaid A, Davey G, Mueller S, Nutt S, Zotos D, et al. Targeting Antigen to Clec9A Primes Follicular Th Cell Memory Responses Capable of Robust Recall. J Immunol. 2015;195:1006-14 pubmed publisher
Krishnamurthy B, Chee J, Jhala G, Trivedi P, Catterall T, Selck C, et al. BIM Deficiency Protects NOD Mice From Diabetes by Diverting Thymocytes to Regulatory T Cells. Diabetes. 2015;64:3229-38 pubmed publisher
Vazquez S, Inlay M, Serwold T. CD201 and CD27 identify hematopoietic stem and progenitor cells across multiple murine strains independently of Kit and Sca-1. Exp Hematol. 2015;43:578-85 pubmed publisher
Schmid C, Robinson M, Scheifinger N, Müller S, Cogliatti S, Tzankov A, et al. DUSP4 deficiency caused by promoter hypermethylation drives JNK signaling and tumor cell survival in diffuse large B cell lymphoma. J Exp Med. 2015;212:775-92 pubmed publisher
Günal Sadık G, Paszkowski Rogacz M, Singaravelu K, Beyer A, Buchholz F, Jessberger R. Stage-specific binding profiles of cohesin in resting and activated B lymphocytes suggest a role for cohesin in immunoglobulin class switching and maturation. PLoS ONE. 2014;9:e111748 pubmed publisher
Soni C, Wong E, Domeier P, Khan T, Satoh T, Akira S, et al. B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. J Immunol. 2014;193:4400-14 pubmed publisher
Ni P, Solomon B, Hsieh C, Allen P, Morris G. The ability to rearrange dual TCRs enhances positive selection, leading to increased Allo- and Autoreactive T cell repertoires. J Immunol. 2014;193:1778-86 pubmed publisher
Simerska P, Suksamran T, Ziora Z, Rivera F, Engwerda C, Toth I. Ovalbumin lipid core peptide vaccines and their CD4(+) and CD8(+) T cell responses. Vaccine. 2014;32:4743-50 pubmed publisher
Bunn P, Stanley A, de Labastida Rivera F, Mulherin A, Sheel M, Alexander C, et al. Tissue requirements for establishing long-term CD4+ T cell-mediated immunity following Leishmania donovani infection. J Immunol. 2014;192:3709-18 pubmed publisher
McPhee C, Bubier J, Sproule T, Park G, Steinbuck M, Schott W, et al. IL-21 is a double-edged sword in the systemic lupus erythematosus-like disease of BXSB.Yaa mice. J Immunol. 2013;191:4581-8 pubmed publisher
Murakami R, Denda Nagai K, Hashimoto S, Nagai S, Hattori M, Irimura T. A unique dermal dendritic cell subset that skews the immune response toward Th2. PLoS ONE. 2013;8:e73270 pubmed publisher
Makdasi E, Eilat D. L chain allelic inclusion does not increase autoreactivity in lupus-prone New Zealand Black/New Zealand White mice. J Immunol. 2013;190:1472-80 pubmed publisher
Nishimori J, Newman T, Oppong G, Rapsinski G, Yen J, Biesecker S, et al. Microbial amyloids induce interleukin 17A (IL-17A) and IL-22 responses via Toll-like receptor 2 activation in the intestinal mucosa. Infect Immun. 2012;80:4398-408 pubmed publisher
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Charles N, Hardwick D, Daugas E, Illei G, Rivera J. Basophils and the T helper 2 environment can promote the development of lupus nephritis. Nat Med. 2010;16:701-7 pubmed publisher
Kat I, Makdasi E, Fischel R, Eilat D. B-cell anergy is maintained in anti-DNA transgenic NZB/NZW mice. Int Immunol. 2010;22:101-11 pubmed publisher
Makdasi E, Fischel R, Kat I, Eilat D. Autoreactive anti-DNA transgenic B cells in lupus-prone New Zealand black/New Zealand white mice show near perfect L chain allelic exclusion. J Immunol. 2009;182:6143-8 pubmed publisher
Lang G, Devera T, Lang M. Requirement for CD1d expression by B cells to stimulate NKT cell-enhanced antibody production. Blood. 2008;111:2158-62 pubmed
product information
Antigen :
CD45R
Apps. Abbrev. :
Flow Cytometry
Cat # :
103227
Clone :
RA3-6B2
Item :
Pacific Blue™ anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cat (Feline)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 , and spatial biology (IBEX) 14,15 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA