product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Cyanine7 anti-mouse/human CD45R/B220
catalog :
103223
quantity :
25 μg
price :
88 USD
clonality :
monoclonal
host :
rat
conjugate :
APC/Cy7
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 89
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:300; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Go D, Lee S, Lee S, Woo S, Kim K, Kim K, et al. Programmed Death Ligand 1-Expressing Classical Dendritic Cells MitigateHelicobacter-Induced Gastritis. Cell Mol Gastroenterol Hepatol. 2021;12:715-739 pubmed publisher
  • flow cytometry; mouse; loading ...
Forbester J, Clement M, Wellington D, Yeung A, Dimonte S, Marsden M, et al. IRF5 Promotes Influenza Virus-Induced Inflammatory Responses in Human Induced Pluripotent Stem Cell-Derived Myeloid Cells and Murine Models. J Virol. 2020;94: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2j, s3k
Kim S, Knight D, Jones L, Vervoort S, Ng A, Seymour J, et al. JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias. Genes Dev. 2018;32:849-864 pubmed publisher
  • flow cytometry; mouse; fig 6a
Aryal B, Rotllan N, Araldi E, Ramírez C, He S, Chousterman B, et al. ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression. Nat Commun. 2016;7:12313 pubmed publisher
  • flow cytometry; mouse
Rodda L, Bannard O, Ludewig B, Nagasawa T, Cyster J. Phenotypic and Morphological Properties of Germinal Center Dark Zone Cxcl12-Expressing Reticular Cells. J Immunol. 2015;195:4781-91 pubmed publisher
Soto Diaz K, Vailati Riboni M, Louie A, McKim D, Gaskins H, Johnson R, et al. Treatment With the CSF1R Antagonist GW2580, Sensitizes Microglia to Reactive Oxygen Species. Front Immunol. 2021;12:734349 pubmed publisher
Lawson H, van de Lagemaat L, Barile M, Tavosanis A, Durko J, Villacreces A, et al. CITED2 coordinates key hematopoietic regulatory pathways to maintain the HSC pool in both steady-state hematopoiesis and transplantation. Stem Cell Reports. 2021;16:2784-2797 pubmed publisher
Rivas M, Durmaz C, Kloetgen A, Chin C, Chen Z, Bhinder B, et al. Cohesin Core Complex Gene Dosage Contributes to Germinal Center Derived Lymphoma Phenotypes and Outcomes. Front Immunol. 2021;12:688493 pubmed publisher
Schäfer A, Eichhorst A, Hentze C, Kraemer A, Amend A, Sprenger D, et al. Low Dietary Fiber Intake Links Development of Obesity and Lupus Pathogenesis. Front Immunol. 2021;12:696810 pubmed publisher
Mathew N, Jayanthan J, Smirnov I, Robinson J, Axelsson H, Nakka S, et al. Single-cell BCR and transcriptome analysis after influenza infection reveals spatiotemporal dynamics of antigen-specific B cells. Cell Rep. 2021;35:109286 pubmed publisher
Rogawski D, Deng J, Li H, Miao H, Borkin D, Purohit T, et al. Discovery of first-in-class inhibitors of ASH1L histone methyltransferase with anti-leukemic activity. Nat Commun. 2021;12:2792 pubmed publisher
Gonzalez Figueroa P, Roco J, Papa I, Núñez Villacís L, Stanley M, Linterman M, et al. Follicular regulatory T cells produce neuritin to regulate B cells. Cell. 2021;184:1775-1789.e19 pubmed publisher
Heyde A, Rohde D, McAlpine C, Zhang S, Hoyer F, Gerold J, et al. Increased stem cell proliferation in atherosclerosis accelerates clonal hematopoiesis. Cell. 2021;184:1348-1361.e22 pubmed publisher
Benet Z, Jing Z, Fooksman D. Plasma cell dynamics in the bone marrow niche. Cell Rep. 2021;34:108733 pubmed publisher
Kovacs S, Oh C, Aachoui Y, Miao E. Evaluating cytokine production by flow cytometry using brefeldin A in mice. STAR Protoc. 2021;2:100244 pubmed publisher
Wang X, Ray R, Kratochvil S, Melzi E, Lin Y, Giguere S, et al. Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptors. EMBO J. 2021;40:e105926 pubmed publisher
Haniuda K, Fukao S, Kitamura D. Metabolic Reprogramming Induces Germinal Center B Cell Differentiation through Bcl6 Locus Remodeling. Cell Rep. 2020;33:108333 pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Fuster J, Zuriaga M, Zorita V, MacLauchlan S, Polackal M, Viana Huete V, et al. TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Rep. 2020;33:108326 pubmed publisher
Kobia F, Preusse K, Dai Q, Weaver N, Hass M, Chaturvedi P, et al. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol. 2020;18:e3000850 pubmed publisher
Zhang C, Xu Z, Yang S, Sun G, Jia L, Zheng Z, et al. tagHi-C Reveals 3D Chromatin Architecture Dynamics during Mouse Hematopoiesis. Cell Rep. 2020;32:108206 pubmed publisher
Venturutti L, Teater M, Zhai A, Chadburn A, Babiker L, Kim D, et al. TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate. Cell. 2020;182:297-316.e27 pubmed publisher
Sarapulov A, Petrov P, Hernández Pérez S, Sustar V, Kuokkanen E, Cords L, et al. Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses. Front Immunol. 2020;11:599 pubmed publisher
Yu X, Dai Y, Zhao Y, Qi S, Liu L, Lu L, et al. Melittin-lipid nanoparticles target to lymph nodes and elicit a systemic anti-tumor immune response. Nat Commun. 2020;11:1110 pubmed publisher
Garcia Fabiani M, Comba A, Kadiyala P, Haase S, Núñez F, Altshuler D, et al. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system. Methods Enzymol. 2020;632:369-388 pubmed publisher
Sullivan J, Tomita Y, Jankowska Gan E, Lema D, Arvedson M, Nair A, et al. Treg-Cell-Derived IL-35-Coated Extracellular Vesicles Promote Infectious Tolerance. Cell Rep. 2020;30:1039-1051.e5 pubmed publisher
Yao M, Ventura P, Jiang Y, Rodriguez F, Wang L, Perry J, et al. Astrocytic trans-Differentiation Completes a Multicellular Paracrine Feedback Loop Required for Medulloblastoma Tumor Growth. Cell. 2020;180:502-520.e19 pubmed publisher
Shinoda K, Maman Y, Canela A, Schatz D, Livak F, Nussenzweig A. Intra-Vκ Cluster Recombination Shapes the Ig Kappa Locus Repertoire. Cell Rep. 2019;29:4471-4481.e6 pubmed publisher
Muri J, Thut H, Bornkamm G, Kopf M. B1 and Marginal Zone B Cells but Not Follicular B2 Cells Require Gpx4 to Prevent Lipid Peroxidation and Ferroptosis. Cell Rep. 2019;29:2731-2744.e4 pubmed publisher
Frodermann V, Rohde D, Courties G, Severe N, Schloss M, Amatullah H, et al. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells. Nat Med. 2019;25:1761-1771 pubmed publisher
Yoshimi A, Lin K, Wiseman D, Rahman M, Pastore A, Wang B, et al. Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis. Nature. 2019;574:273-277 pubmed publisher
Viny A, Bowman R, Liu Y, Lavallée V, Eisman S, Xiao W, et al. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation. Cell Stem Cell. 2019;25:682-696.e8 pubmed publisher
Wang Y, Tsai D, Ko Y, Yang T, Lin I, Hung K, et al. Blimp-1 Contributes to the Development and Function of Regulatory B Cells. Front Immunol. 2019;10:1909 pubmed publisher
Sasaki K, Himeno A, Nakagawa T, Sasaki Y, Kiyonari H, Iwai K. Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death. Nat Commun. 2019;10:3878 pubmed publisher
Roco J, Mesin L, Binder S, Nefzger C, Gonzalez Figueroa P, Canete P, et al. Class-Switch Recombination Occurs Infrequently in Germinal Centers. Immunity. 2019;51:337-350.e7 pubmed publisher
Nowlan B, Williams E, Doran M, Levesque J. CD27, CD201, FLT3, CD48, and CD150 cell surface staining identifies long-term mouse hematopoietic stem cells in immunodeficient non-obese diabetic severe combined immune deficient derived strains. Haematologica. 2019;: pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Isvoranu G, Surcel M, Huică R, Munteanu A, Pirvu I, Ciotaru D, et al. Natural killer cell monitoring in cutaneous melanoma - new dynamic biomarker. Oncol Lett. 2019;17:4197-4206 pubmed publisher
Roy K, Mitchell S, Liu Y, Ohta S, Lin Y, Metzig M, et al. A Regulatory Circuit Controlling the Dynamics of NFκB cRel Transitions B Cells from Proliferation to Plasma Cell Differentiation. Immunity. 2019;50:616-628.e6 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Cantor D, King B, Blumenberg L, Dimauro T, Aifantis I, Koralov S, et al. Impaired Expression of Rearranged Immunoglobulin Genes and Premature p53 Activation Block B Cell Development in BMI1 Null Mice. Cell Rep. 2019;26:108-118.e4 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Celik H, Koh W, Kramer A, Ostrander E, Mallaney C, Fisher D, et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell. 2018;34:741-756.e8 pubmed publisher
Sun Y, Zhou B, Mao F, Xu J, Miao H, Zou Z, et al. HOXA9 Reprograms the Enhancer Landscape to Promote Leukemogenesis. Cancer Cell. 2018;34:643-658.e5 pubmed publisher
Stewart I, Radtke D, Phillips B, McGowan S, Bannard O. Germinal Center B Cells Replace Their Antigen Receptors in Dark Zones and Fail Light Zone Entry when Immunoglobulin Gene Mutations are Damaging. Immunity. 2018;49:477-489.e7 pubmed publisher
Lam W, Jash A, Yao C, D Souza L, Wong R, Nunley R, et al. Metabolic and Transcriptional Modules Independently Diversify Plasma Cell Lifespan and Function. Cell Rep. 2018;24:2479-2492.e6 pubmed publisher
Xiao G, Chan L, Klemm L, Braas D, Chen Z, Geng H, et al. B-Cell-Specific Diversion of Glucose Carbon Utilization Reveals a Unique Vulnerability in B Cell Malignancies. Cell. 2018;173:470-484.e18 pubmed publisher
Uribe Herranz M, Bittinger K, Rafail S, Guedan S, Pierini S, Tanes C, et al. Gut microbiota modulates adoptive cell therapy via CD8α dendritic cells and IL-12. JCI Insight. 2018;3: pubmed publisher
Kunimoto H, Meydan C, Nazir A, Whitfield J, Shank K, Rapaport F, et al. Cooperative Epigenetic Remodeling by TET2 Loss and NRAS Mutation Drives Myeloid Transformation and MEK Inhibitor Sensitivity. Cancer Cell. 2018;33:44-59.e8 pubmed publisher
Petursdottir D, Nordlander S, Qazi K, Carvalho Queiroz C, Ahmed Osman O, Hell E, et al. Early-Life Human Microbiota Associated With Childhood Allergy Promotes the T Helper 17 Axis in Mice. Front Immunol. 2017;8:1699 pubmed publisher
Kleppe M, Koche R, Zou L, van Galen P, Hill C, Dong L, et al. Dual Targeting of Oncogenic Activation and Inflammatory Signaling Increases Therapeutic Efficacy in Myeloproliferative Neoplasms. Cancer Cell. 2018;33:29-43.e7 pubmed publisher
Chen X, Deng H, Churchill M, Luchsinger L, Du X, Chu T, et al. Bone Marrow Myeloid Cells Regulate Myeloid-Biased Hematopoietic Stem Cells via a Histamine-Dependent Feedback Loop. Cell Stem Cell. 2017;21:747-760.e7 pubmed publisher
Bowers E, Slaughter A, Frenette P, Kuick R, Pello O, Lucas D. Granulocyte-derived TNFα promotes vascular and hematopoietic regeneration in the bone marrow. Nat Med. 2018;24:95-102 pubmed publisher
Whiteley A, Prado M, Peng I, Abbas A, Haley B, Paulo J, et al. Ubiquilin1 promotes antigen-receptor mediated proliferation by eliminating mislocalized mitochondrial proteins. elife. 2017;6: pubmed publisher
Furuta Y, Tsai S, Kinoshita M, Fujimoto K, Okumura R, Umemoto E, et al. E-NPP3 controls plasmacytoid dendritic cell numbers in the small intestine. PLoS ONE. 2017;12:e0172509 pubmed publisher
Laidlaw B, Schmidt T, Green J, Allen C, Okada T, Cyster J. The Eph-related tyrosine kinase ligand Ephrin-B1 marks germinal center and memory precursor B cells. J Exp Med. 2017;214:639-649 pubmed publisher
St Clair J, Detanico T, Aviszus K, Kirchenbaum G, Christie M, Carpenter J, et al. Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes. PLoS ONE. 2017;12:e0170556 pubmed publisher
Li Y, Xu S, Huang Y, Ou X, Lam K. Tyrosine kinase c-Abl regulates the survival of plasma cells. Sci Rep. 2017;7:40133 pubmed publisher
Sochalska M, Schuler F, Weiss J, Prchal Murphy M, Sexl V, Villunger A. MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis. Oncogene. 2017;36:2066-2073 pubmed publisher
Roufaiel M, Gracey E, Siu A, Zhu S, Lau A, Ibrahim H, et al. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima. Nat Immunol. 2016;17:1263-1272 pubmed publisher
Jacoby E, Nguyen S, Fountaine T, Welp K, Gryder B, Qin H, et al. CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity. Nat Commun. 2016;7:12320 pubmed publisher
Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher
Ishikura S, Iwaihara Y, Tanaka Y, Luo H, Nishi K, Doi K, et al. The Nuclear Zinc Finger Protein Zfat Maintains FoxO1 Protein Levels in Peripheral T Cells by Regulating the Activities of Autophagy and the Akt Signaling Pathway. J Biol Chem. 2016;291:15282-91 pubmed publisher
Georgoudaki A, Prokopec K, Boura V, Hellqvist E, Sohn S, Östling J, et al. Reprogramming Tumor-Associated Macrophages by Antibody Targeting Inhibits Cancer Progression and Metastasis. Cell Rep. 2016;15:2000-11 pubmed publisher
Andresen L, Theodorou K, Grünewald S, Czech Zechmeister B, Könnecke B, Luhder F, et al. Evaluation of the Therapeutic Potential of Anti-TLR4-Antibody MTS510 in Experimental Stroke and Significance of Different Routes of Application. PLoS ONE. 2016;11:e0148428 pubmed publisher
Palm A, García Faroldi G, Lundberg M, Pejler G, Kleinau S. Activated mast cells promote differentiation of B cells into effector cells. Sci Rep. 2016;6:20531 pubmed publisher
Montes De Oca M, Kumar R, de Labastida Rivera F, Amante F, Sheel M, Faleiro R, et al. Blimp-1-Dependent IL-10 Production by Tr1 Cells Regulates TNF-Mediated Tissue Pathology. PLoS Pathog. 2016;12:e1005398 pubmed publisher
Jacoby E, Yang Y, Qin H, Chien C, Kochenderfer J, Fry T. Murine allogeneic CD19 CAR T cells harbor potent antileukemic activity but have the potential to mediate lethal GVHD. Blood. 2016;127:1361-70 pubmed publisher
He Z, O Neal J, Wilson W, Mahajan N, Luo J, Wang Y, et al. Deletion of Rb1 induces both hyperproliferation and cell death in murine germinal center B cells. Exp Hematol. 2016;44:161-5.e4 pubmed publisher
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Muppidi J, Lu E, Cyster J. The G protein-coupled receptor P2RY8 and follicular dendritic cells promote germinal center confinement of B cells, whereas S1PR3 can contribute to their dissemination. J Exp Med. 2015;212:2213-22 pubmed publisher
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product information
Antigen :
CD45R
Apps. Abbrev. :
Flow Cytometry
Cat # :
103223
Clone :
RA3-6B2
Item :
APC/Cyanine7 anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
25 μg
Price (USD) :
88 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cat (Feline)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_CYANINE7
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 , and spatial biology (IBEX) 14,15 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA