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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE/Cyanine7 anti-mouse/human CD45R/B220
catalog :
103221
quantity :
25 μg
price :
88 USD
clonality :
monoclonal
host :
rat
conjugate :
PE/Cy7
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 79
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; fig 2a
Bohannon C, Ende Z, Cao W, Mboko W, Ranjan P, Kumar A, et al. Influenza Virus Infects and Depletes Activated Adaptive Immune Responders. Adv Sci (Weinh). 2021;8:e2100693 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3
Barker K, Etesami N, Shenoy A, Arafa E, Lyon de Ana C, Smith N, et al. Lung-resident memory B cells protect against bacterial pneumonia. J Clin Invest. 2021;131: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a, 4a
Mantani P, Dunér P, Ljungcrantz I, Nilsson J, Bjorkbacka H, Fredrikson G. ILC2 transfers to apolipoprotein E deficient mice reduce the lipid content of atherosclerotic lesions. BMC Immunol. 2019;20:47 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s8b
Li Y, Tinoco R, Elmén L, Segota I, Xian Y, Fujita Y, et al. Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5-/- mice. Nat Commun. 2019;10:1492 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 1e
Grootjans J, Krupka N, Hosomi S, Matute J, Hanley T, Saveljeva S, et al. Epithelial endoplasmic reticulum stress orchestrates a protective IgA response. Science. 2019;363:993-998 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 1c
Sang A, Danhorn T, Peterson J, Rankin A, O Connor B, Leach S, et al. Innate and adaptive signals enhance differentiation and expansion of dual-antibody autoreactive B cells in lupus. Nat Commun. 2018;9:3973 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 1a
Sakamoto A, Matsuda T, Kawaguchi K, Takaoka A, Maruyama M. Involvement of Zizimin2/3 in the age-related defect of peritoneal B-1a cells as a source of anti-bacterial IgM. Int Immunol. 2017;29:431-438 pubmed publisher
  • flow cytometry; mouse; loading ...; fig st2
Albarrán Juárez J, Kaur H, Grimm M, Offermanns S, Wettschureck N. Lineage tracing of cells involved in atherosclerosis. Atherosclerosis. 2016;251:445-453 pubmed publisher
  • flow cytometry; mouse; fig 3
Rozanski C, Utley A, Carlson L, Farren M, Murray M, Russell L, et al. CD28 Promotes Plasma Cell Survival, Sustained Antibody Responses, and BLIMP-1 Upregulation through Its Distal PYAP Proline Motif. J Immunol. 2015;194:4717-28 pubmed publisher
  • flow cytometry; mouse; tbl s1
Sakamoto H, Takeda N, Arai F, Hosokawa K, García P, Suda T, et al. Determining c-Myb protein levels can isolate functional hematopoietic stem cell subtypes. Stem Cells. 2015;33:479-90 pubmed publisher
  • flow cytometry; mouse; fig s1
Stoilova B, Kowenz Leutz E, Scheller M, Leutz A. Lymphoid to myeloid cell trans-differentiation is determined by C/EBP? structure and post-translational modifications. PLoS ONE. 2013;8:e65169 pubmed publisher
Leary N, Walser S, He Y, Cousin N, Pereira P, Gallo A, et al. Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes. J Extracell Vesicles. 2022;11:e12197 pubmed publisher
Li Y, Wang H, Zhang Z, Tang C, Zhou X, Mohan C, et al. Identification of polo-like kinase 1 as a therapeutic target in murine lupus. Clin Transl Immunology. 2022;11:e1362 pubmed publisher
Read B, Won L, Kraft J, Sappington I, Aung A, Wu S, et al. Mannose-binding lectin and complement mediate follicular localization and enhanced immunogenicity of diverse protein nanoparticle immunogens. Cell Rep. 2022;38:110217 pubmed publisher
Ohyagi M, Nagata T, Ihara K, Yoshida Tanaka K, Nishi R, Miyata H, et al. DNA/RNA heteroduplex oligonucleotide technology for regulating lymphocytes in vivo. Nat Commun. 2021;12:7344 pubmed publisher
Rappe J, Finsterbusch K, Crotta S, Mack M, Priestnall S, Wack A. A TLR7 antagonist restricts interferon-dependent and -independent immunopathology in a mouse model of severe influenza. J Exp Med. 2021;218: pubmed publisher
Mathew N, Jayanthan J, Smirnov I, Robinson J, Axelsson H, Nakka S, et al. Single-cell BCR and transcriptome analysis after influenza infection reveals spatiotemporal dynamics of antigen-specific B cells. Cell Rep. 2021;35:109286 pubmed publisher
Volberding P, Xin G, Kasmani M, Khatun A, Brown A, Nguyen C, et al. Suppressive neutrophils require PIM1 for metabolic fitness and survival during chronic viral infection. Cell Rep. 2021;35:109160 pubmed publisher
Wang W, Li R, Miao W, Evans C, Lu L, Lyu J, et al. Development and Evaluation of a Novel Mouse Model of Asphyxial Cardiac Arrest Revealed Severely Impaired Lymphopoiesis After Resuscitation. J Am Heart Assoc. 2021;10:e019142 pubmed publisher
Yi W, Zhang Y, Liu B, Zhou Y, Liao D, Qiao X, et al. Protein S-nitrosylation regulates proteostasis and viability of hematopoietic stem cell during regeneration. Cell Rep. 2021;34:108922 pubmed publisher
Suzuki M, Sujino T, Chiba S, Harada Y, Goto M, Takahashi R, et al. Host-microbe cross-talk governs amino acid chirality to regulate survival and differentiation of B cells. Sci Adv. 2021;7: pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
Trefzer A, Kadam P, Wang S, Pennavaria S, Lober B, Akçabozan B, et al. Dynamic adoption of anergy by antigen-exhausted CD4+ T cells. Cell Rep. 2021;34:108748 pubmed publisher
Nagamachi A, Kanai A, Nakamura M, Okuda H, Yokoyama A, Shinriki S, et al. Multiorgan failure with abnormal receptor metabolism in mice mimicking Samd9/9L syndromes. J Clin Invest. 2021;131: pubmed publisher
Alexandre Y, Devi S, Park S, Mackay L, Heath W, Mueller S. Systemic Inflammation Suppresses Lymphoid Tissue Remodeling and B Cell Immunity during Concomitant Local Infection. Cell Rep. 2020;33:108567 pubmed publisher
Fennell L, Gómez Diaz C, Deszcz L, Kavirayani A, Hoffmann D, Yanagitani K, et al. Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling. EMBO J. 2020;:e103303 pubmed publisher
Wanner Seleznik G, Reding T, Chen R, Gupta A, Lenggenhager D, Browning J, et al. Amelioration of Murine Autoimmune Pancreatitis by Targeted LTβR Inhibition and Anti-CD20 Treatment. Immunohorizons. 2020;4:688-700 pubmed publisher
Haniuda K, Fukao S, Kitamura D. Metabolic Reprogramming Induces Germinal Center B Cell Differentiation through Bcl6 Locus Remodeling. Cell Rep. 2020;33:108333 pubmed publisher
Crouse B, Robinson C, Huseby Kelcher A, Laudenbach M, Abrahante J, Pravetoni M. Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders. NPJ Vaccines. 2020;5:99 pubmed publisher
Kobia F, Preusse K, Dai Q, Weaver N, Hass M, Chaturvedi P, et al. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol. 2020;18:e3000850 pubmed publisher
Li X, Wu Y, Song Y, Ding N, Lu M, Jia L, et al. Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes. Mol Ther. 2020;28:2430-2441 pubmed publisher
Manion K, Baglaenko Y, Chang N, Talaei N, Wither J. Impaired B cell anergy is not sufficient to breach tolerance to nuclear antigen in Vκ8/3H9 lupus-prone mice. PLoS ONE. 2020;15:e0236664 pubmed publisher
Tran N, Trombke J, Rajewsky K, Chu V. Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells. STAR Protoc. 2020;1:100028 pubmed publisher
Ramanan D, Sefik E, Galván Peña S, Wu M, Yang L, Yang Z, et al. An Immunologic Mode of Multigenerational Transmission Governs a Gut Treg Setpoint. Cell. 2020;181:1276-1290.e13 pubmed publisher
Satoh Takayama N, Kato T, Motomura Y, Kageyama T, Taguchi Atarashi N, Kinoshita Daitoku R, et al. Bacteria-Induced Group 2 Innate Lymphoid Cells in the Stomach Provide Immune Protection through Induction of IgA. Immunity. 2020;52:635-649.e4 pubmed publisher
Li Y, Elmén L, Segota I, Xian Y, Tinoco R, Feng Y, et al. Prebiotic-Induced Anti-tumor Immunity Attenuates Tumor Growth. Cell Rep. 2020;30:1753-1766.e6 pubmed publisher
Terashima Y, Toda E, Itakura M, Otsuji M, Yoshinaga S, Okumura K, et al. Targeting FROUNT with disulfiram suppresses macrophage accumulation and its tumor-promoting properties. Nat Commun. 2020;11:609 pubmed publisher
Fallet B, Hao Y, Florova M, Cornille K, de Los Aires A, Girelli Zubani G, et al. Chronic Viral Infection Promotes Efficient Germinal Center B Cell Responses. Cell Rep. 2020;30:1013-1026.e7 pubmed publisher
Paubelle E, Zylbersztejn F, Maciel T, Carvalho C, Mupo A, Cheok M, et al. Vitamin D Receptor Controls Cell Stemness in Acute Myeloid Leukemia and in Normal Bone Marrow. Cell Rep. 2020;30:739-754.e4 pubmed publisher
Fukushima T, Tanaka Y, Hamey F, Chang C, Oki T, Asada S, et al. Discrimination of Dormant and Active Hematopoietic Stem Cells by G0 Marker Reveals Dormancy Regulation by Cytoplasmic Calcium. Cell Rep. 2019;29:4144-4158.e7 pubmed publisher
Frodermann V, Rohde D, Courties G, Severe N, Schloss M, Amatullah H, et al. Exercise reduces inflammatory cell production and cardiovascular inflammation via instruction of hematopoietic progenitor cells. Nat Med. 2019;25:1761-1771 pubmed publisher
Tran N, Sommermann T, Graf R, Trombke J, Pempe J, Petsch K, et al. Efficient CRISPR/Cas9-Mediated Gene Knockin in Mouse Hematopoietic Stem and Progenitor Cells. Cell Rep. 2019;28:3510-3522.e5 pubmed publisher
Shehata L, Maurer D, Wec A, Lilov A, Champney E, Sun T, et al. Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability. Cell Rep. 2019;28:3300-3308.e4 pubmed publisher
Viny A, Bowman R, Liu Y, Lavallée V, Eisman S, Xiao W, et al. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation. Cell Stem Cell. 2019;25:682-696.e8 pubmed publisher
Di Mitri D, Mirenda M, Vasilevska J, Calcinotto A, Delaleu N, Revandkar A, et al. Re-education of Tumor-Associated Macrophages by CXCR2 Blockade Drives Senescence and Tumor Inhibition in Advanced Prostate Cancer. Cell Rep. 2019;28:2156-2168.e5 pubmed publisher
Lu X, Chu C, Fang T, Rayon Estrada V, Fang F, Patke A, et al. MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition. Cell Rep. 2019;28:472-485.e5 pubmed publisher
Liu D, Yin X, Olyha S, Nascimento M, Chen P, White T, et al. IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α+ Dendritic Cells Promotes Listeria monocytogenes Infection. Immunity. 2019;: pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
Zhu H, Bhatt B, Sivaprakasam S, Cai Y, Liu S, Kodeboyina S, et al. Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR. Nat Commun. 2019;10:1084 pubmed publisher
Farsakoglu Y, Palomino Segura M, Latino I, Zanaga S, Chatziandreou N, Pizzagalli D, et al. Influenza Vaccination Induces NK-Cell-Mediated Type-II IFN Response that Regulates Humoral Immunity in an IL-6-Dependent Manner. Cell Rep. 2019;26:2307-2315.e5 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
Kiyohara H, Sujino T, Teratani T, Miyamoto K, Arai M, Nomura E, et al. Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. Cell Mol Gastroenterol Hepatol. 2019;7:135-156 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Celik H, Koh W, Kramer A, Ostrander E, Mallaney C, Fisher D, et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell. 2018;34:741-756.e8 pubmed publisher
Shan M, Carrillo J, Yeste A, Gutzeit C, Segura Garzón D, Walland A, et al. Secreted IgD Amplifies Humoral T Helper 2 Cell Responses by Binding Basophils via Galectin-9 and CD44. Immunity. 2018;49:709-724.e8 pubmed publisher
Sharma P, Dmitriev I, Kashentseva E, Raes G, Li L, Kim S, et al. Development of an adenovirus vector vaccine platform for targeting dendritic cells. Cancer Gene Ther. 2018;25:27-38 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
El Zaatari M, Bass A, Bowlby R, Zhang M, Syu L, Yang Y, et al. Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity. Gastroenterology. 2018;154:140-153.e17 pubmed publisher
Perez Mazliah D, Nguyen M, Hosking C, McLaughlin S, Lewis M, Tumwine I, et al. Follicular Helper T Cells are Essential for the Elimination of Plasmodium Infection. EBioMedicine. 2017;24:216-230 pubmed publisher
Ersching J, Efeyan A, Mesin L, Jacobsen J, Pasqual G, Grabiner B, et al. Germinal Center Selection and Affinity Maturation Require Dynamic Regulation of mTORC1 Kinase. Immunity. 2017;46:1045-1058.e6 pubmed publisher
Timilshina M, Kang Y, Dahal I, You Z, Nam T, Kim K, et al. BJ-3105, a 6-Alkoxypyridin-3-ol Analog, Impairs T Cell Differentiation and Prevents Experimental Autoimmune Encephalomyelitis Disease Progression. PLoS ONE. 2017;12:e0168942 pubmed publisher
Miyauchi K, Sugimoto Ishige A, Harada Y, Adachi Y, Usami Y, Kaji T, et al. Protective neutralizing influenza antibody response in the absence of T follicular helper cells. Nat Immunol. 2016;17:1447-1458 pubmed publisher
Matsushita N, Suzuki M, Ikebe E, Nagashima S, Inatome R, Asano K, et al. Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1. Sci Rep. 2016;6:31266 pubmed publisher
Ulrich V, Rotllan N, Araldi E, Luciano A, Skroblin P, Abonnenc M, et al. Chronic miR-29 antagonism promotes favorable plaque remodeling in atherosclerotic mice. EMBO Mol Med. 2016;8:643-53 pubmed publisher
Valkenburg S, Mallajosyula V, Li O, Chin A, Carnell G, Temperton N, et al. Stalking influenza by vaccination with pre-fusion headless HA mini-stem. Sci Rep. 2016;6:22666 pubmed publisher
Xie X, Yang P, Zhang Y, Zhang P, Wang L, Ding Y, et al. Discovery of novel INK4C small-molecule inhibitors to promote human and murine hematopoietic stem cell ex vivo expansion. Sci Rep. 2015;5:18115 pubmed publisher
Tozuka M, Oka T, Jounai N, Egawa G, Ishii K, Kabashima K, et al. Efficient antigen delivery to the draining lymph nodes is a key component in the immunogenic pathway of the intradermal vaccine. J Dermatol Sci. 2016;82:38-45 pubmed publisher
Richardson E, Shukla S, Nagy N, Boom W, Beck R, Zhou L, et al. ERK Signaling Is Essential for Macrophage Development. PLoS ONE. 2015;10:e0140064 pubmed publisher
Burrack K, Tan J, McCarthy M, Her Z, Berger J, Ng L, et al. Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells. PLoS Pathog. 2015;11:e1005191 pubmed publisher
Burrack K, Montgomery S, Homann D, Morrison T. CD8+ T cells control Ross River virus infection in musculoskeletal tissues of infected mice. J Immunol. 2015;194:678-89 pubmed publisher
Heizmann B, Kastner P, Chan S. Ikaros is absolutely required for pre-B cell differentiation by attenuating IL-7 signals. J Exp Med. 2013;210:2823-32 pubmed publisher
Murakami R, Denda Nagai K, Hashimoto S, Nagai S, Hattori M, Irimura T. A unique dermal dendritic cell subset that skews the immune response toward Th2. PLoS ONE. 2013;8:e73270 pubmed publisher
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product information
Antigen :
CD45R
Apps. Abbrev. :
Flow Cytometry
Cat # :
103221
Clone :
RA3-6B2
Item :
PE/Cyanine7 anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
25 μg
Price (USD) :
88 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cat (Feline)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE_CYANINE7
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 , and spatial biology (IBEX) 14,15 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA