product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse/human CD45R/B220
catalog :
103211
quantity :
25 μg
price :
25 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 92
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2j, s3k
Kim S, Knight D, Jones L, Vervoort S, Ng A, Seymour J, et al. JAK2 is dispensable for maintenance of JAK2 mutant B-cell acute lymphoblastic leukemias. Genes Dev. 2018;32:849-864 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s5b
Mazor R, King E, Onda M, Cuburu N, Addissie S, Crown D, et al. Tolerogenic nanoparticles restore the antitumor activity of recombinant immunotoxins by mitigating immunogenicity. Proc Natl Acad Sci U S A. 2018;115:E733-E742 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Kurkewich J, Boucher A, Klopfenstein N, Baskar R, Kapur R, Dahl R. The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation. Exp Hematol. 2018;59:14-29 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 1a
Sakamoto A, Matsuda T, Kawaguchi K, Takaoka A, Maruyama M. Involvement of Zizimin2/3 in the age-related defect of peritoneal B-1a cells as a source of anti-bacterial IgM. Int Immunol. 2017;29:431-438 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3c
Damle S, Martin R, Cross J, Conrad D. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis. Mucosal Immunol. 2017;10:205-214 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Kurkewich J, Bikorimana E, Nguyen T, Klopfenstein N, Zhang H, Hallas W, et al. The mirn23a microRNA cluster antagonizes B cell development. J Leukoc Biol. 2016;100:665-677 pubmed
  • flow cytometry; mouse
Catarinella M, Monestiroli A, Escobar G, Fiocchi A, Tran N, Aiolfi R, et al. IFNα gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment. EMBO Mol Med. 2016;8:155-70 pubmed publisher
  • flow cytometry; mouse; fig 5
Kim Y, Lim H, Jung H, Wetsel R, Chung Y. Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS ONE. 2015;10:e0120294 pubmed publisher
  • flow cytometry; mouse; 1:160; fig 4
Kishimoto M, Matsuda T, Yanase S, Katsumi A, Suzuki N, Ikejiri M, et al. Rhof promotes murine marginal zone B cell development. Nagoya J Med Sci. 2014;76:293-305 pubmed
  • flow cytometry; mouse; 1:160; fig 7
Matsuda T, Yanase S, Takaoka A, Maruyama M. The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation. Immun Ageing. 2015;12:1 pubmed publisher
Kim C, Jadhav R, Gustafson C, Smithey M, Hirsch A, Uhrlaub J, et al. Defects in Antiviral T Cell Responses Inflicted by Aging-Associated miR-181a Deficiency. Cell Rep. 2019;29:2202-2216.e5 pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Finkin S, Hartweger H, Oliveira T, Kara E, Nussenzweig M. Protein Amounts of the MYC Transcription Factor Determine Germinal Center B Cell Division Capacity. Immunity. 2019;51:324-336.e5 pubmed publisher
Hou X, Chen G, Bracamonte Baran W, Choi H, Diny N, Sung J, et al. The Cardiac Microenvironment Instructs Divergent Monocyte Fates and Functions in Myocarditis. Cell Rep. 2019;28:172-189.e7 pubmed publisher
Kobayashi H, Morikawa T, Okinaga A, Hamano F, Hashidate Yoshida T, Watanuki S, et al. Environmental Optimization Enables Maintenance of Quiescent Hematopoietic Stem Cells Ex Vivo. Cell Rep. 2019;28:145-158.e9 pubmed publisher
Perruzza L, Strati F, Gargari G, D Erchia A, Fosso B, Pesole G, et al. Enrichment of intestinal Lactobacillus by enhanced secretory IgA coating alters glucose homeostasis in P2rx7-/- mice. Sci Rep. 2019;9:9315 pubmed publisher
Rothweiler S, Feldbrügge L, Jiang Z, Csizmadia E, Longhi M, Vaid K, et al. Selective deletion of ENTPD1/CD39 in macrophages exacerbates biliary fibrosis in a mouse model of sclerosing cholangitis. Purinergic Signal. 2019;15:375-385 pubmed publisher
Radulović V, van der Garde M, Koide S, Sigurdsson V, Lang S, Kaneko S, et al. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis. Cell Rep. 2019;27:2826-2836.e5 pubmed publisher
Escolano A, Gristick H, Abernathy M, Merkenschlager J, Gautam R, Oliveira T, et al. Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques. Nature. 2019;: pubmed publisher
Hu J, Xu J, Li M, Zhang Y, Yi H, Chen J, et al. Targeting Lymph Node Sinus Macrophages to Inhibit Lymph Node Metastasis. Mol Ther Nucleic Acids. 2019;16:650-662 pubmed publisher
Perper S, Westmoreland S, Karman J, Twomey R, Seagal J, Wang R, et al. Treatment with a CD40 Antagonist Antibody Reverses Severe Proteinuria and Loss of Saliva Production and Restores Glomerular Morphology in Murine Systemic Lupus Erythematosus. J Immunol. 2019;: pubmed publisher
Yue X, Lio C, Samaniego Castruita D, Li X, Rao A. Loss of TET2 and TET3 in regulatory T cells unleashes effector function. Nat Commun. 2019;10:2011 pubmed publisher
Wilson R, Tursi S, Rapsinski G, Medeiros N, Le L, Kotredes K, et al. STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium. PLoS Pathog. 2019;15:e1007745 pubmed publisher
Delás M, Jackson B, Kovacević T, Vangelisti S, Munera Maravilla E, Wild S, et al. lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation. Cell Rep. 2019;27:719-729.e6 pubmed publisher
Kubota S, Tokunaga K, Umezu T, Yokomizo Nakano T, Sun Y, Oshima M, et al. Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm. Nat Commun. 2019;10:1653 pubmed publisher
Graf R, Li X, Chu V, Rajewsky K. sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing. Cell Rep. 2019;26:1098-1103.e3 pubmed publisher
Luo H, Mu W, Karki R, Chiang H, Mohrin M, Shin J, et al. Mitochondrial Stress-Initiated Aberrant Activation of the NLRP3 Inflammasome Regulates the Functional Deterioration of Hematopoietic Stem Cell Aging. Cell Rep. 2019;26:945-954.e4 pubmed publisher
Proietti M, Perruzza L, Scribano D, Pellegrini G, D Antuono R, Strati F, et al. ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens. Nat Commun. 2019;10:250 pubmed publisher
Bussey K, Murthy S, Reimer E, Chan B, Hatesuer B, Schughart K, et al. Endosomal Toll-Like Receptors 7 and 9 Cooperate in Detection of Murine Gammaherpesvirus 68 Infection. J Virol. 2019;93: pubmed publisher
Ovadya Y, Landsberger T, Leins H, Vadai E, Gal H, Biran A, et al. Impaired immune surveillance accelerates accumulation of senescent cells and aging. Nat Commun. 2018;9:5435 pubmed publisher
Chen J, Li N, Yin Y, Zheng N, Min M, Lin B, et al. Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells. Cell Rep. 2018;25:3393-3404.e6 pubmed publisher
Säwén P, Eldeeb M, Erlandsson E, Kristiansen T, Laterza C, Kokaia Z, et al. Murine HSCs contribute actively to native hematopoiesis but with reduced differentiation capacity upon aging. elife. 2018;7: pubmed publisher
Celik H, Koh W, Kramer A, Ostrander E, Mallaney C, Fisher D, et al. JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Cancer Cell. 2018;34:741-756.e8 pubmed publisher
Liu S, Xia Q, Wu X, Sun F, Hu Q, Wu J, et al. Stimulator of Interferon Genes in Classical Dendritic Cells Controls Mucosal Th17 Responses to Cyclic Dinucleotides for Host Defenses Against Microbial Infections in Gut. Front Immunol. 2018;9:1085 pubmed publisher
Iwamoto H, Abe M, Yang Y, Cui D, Seki T, Nakamura M, et al. Cancer Lipid Metabolism Confers Antiangiogenic Drug Resistance. Cell Metab. 2018;28:104-117.e5 pubmed publisher
Li X, Gadzinsky A, Gong L, Tong H, Calderon V, Li Y, et al. Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction. Immunity. 2018;48:530-541.e6 pubmed publisher
Laban H, Weigert A, Zink J, Elgheznawy A, Schürmann C, Günther L, et al. VASP regulates leukocyte infiltration, polarization, and vascular repair after ischemia. J Cell Biol. 2018;217:1503-1519 pubmed publisher
Booth C, Barkas N, Neo W, Boukarabila H, Soilleux E, Giotopoulos G, et al. Ezh2 and Runx1 Mutations Collaborate to Initiate Lympho-Myeloid Leukemia in Early Thymic Progenitors. Cancer Cell. 2018;33:274-291.e8 pubmed publisher
Mrdjen D, Pavlovic A, Hartmann F, Schreiner B, Utz S, Leung B, et al. High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease. Immunity. 2018;48:380-395.e6 pubmed publisher
Emgård J, Kammoun H, García Cassani B, Chesne J, Parigi S, Jacob J, et al. Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation. Immunity. 2018;48:120-132.e8 pubmed publisher
Neumann A, Abele J, Kirschstein T, Engelmann R, Sellmann T, Köhling R, et al. Mycophenolate mofetil prevents the delayed T cell response after pilocarpine-induced status epilepticus in mice. PLoS ONE. 2017;12:e0187330 pubmed publisher
Hönes J, Thivakaran A, Botezatu L, Patnana P, Castro S, Al Matary Y, et al. Enforced GFI1 expression impedes human and murine leukemic cell growth. Sci Rep. 2017;7:15720 pubmed publisher
Zhu D, Tan J, Maleken A, Muljadi R, Chan S, Lau S, et al. Human amnion cells reverse acute and chronic pulmonary damage in experimental neonatal lung injury. Stem Cell Res Ther. 2017;8:257 pubmed publisher
Prajeeth C, Kronisch J, Khorooshi R, Knier B, Toft Hansen H, Gudi V, et al. Effectors of Th1 and Th17 cells act on astrocytes and augment their neuroinflammatory properties. J Neuroinflammation. 2017;14:204 pubmed publisher
Tanegashima K, Takahashi R, Nuriya H, Iwase R, Naruse N, Tsuji K, et al. CXCL14 Acts as a Specific Carrier of CpG DNA into Dendritic Cells and Activates Toll-like Receptor 9-mediated Adaptive Immunity. EBioMedicine. 2017;24:247-256 pubmed publisher
Riffelmacher T, Clarke A, Richter F, Stranks A, Pandey S, Danielli S, et al. Autophagy-Dependent Generation of Free Fatty Acids Is Critical for Normal Neutrophil Differentiation. Immunity. 2017;47:466-480.e5 pubmed publisher
Ishidome T, Yoshida T, Hanayama R. Induction of Live Cell Phagocytosis by a Specific Combination of Inflammatory Stimuli. EBioMedicine. 2017;22:89-99 pubmed publisher
Sato K, Kato A, Sekai M, Hamazaki Y, Minato N. Physiologic Thymic Involution Underlies Age-Dependent Accumulation of Senescence-Associated CD4+ T Cells. J Immunol. 2017;199:138-148 pubmed publisher
Perfilyeva Y, Abdolla N, Ostapchuk Y, Tleulieva R, Krasnoshtanov V, Belyaev N. Expansion of CD11b+Ly6Ghigh and CD11b+CD49d+ myeloid cells with suppressive potential in mice with chronic inflammation and light-at-night-induced circadian disruption. Inflamm Res. 2017;66:711-724 pubmed publisher
Le Noir S, Boyer F, Lecardeur S, Brousse M, Oruc Z, Cook Moreau J, et al. Functional anatomy of the immunoglobulin heavy chain 3? super-enhancer needs not only core enhancer elements but also their unique DNA context. Nucleic Acids Res. 2017;45:5829-5837 pubmed publisher
St Clair J, Detanico T, Aviszus K, Kirchenbaum G, Christie M, Carpenter J, et al. Immunogenicity of Isogenic IgG in Aggregates and Immune Complexes. PLoS ONE. 2017;12:e0170556 pubmed publisher
Kurkewich J, Klopfenstein N, Hallas W, Wood C, Sattler R, Das C, et al. Arid3b Is Critical for B Lymphocyte Development. PLoS ONE. 2016;11:e0161468 pubmed publisher
Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher
Novković M, Onder L, Cupovic J, Abe J, Bomze D, Cremasco V, et al. Topological Small-World Organization of the Fibroblastic Reticular Cell Network Determines Lymph Node Functionality. PLoS Biol. 2016;14:e1002515 pubmed publisher
Hohmann A, Martin L, Minder J, Roe J, Shi J, Steurer S, et al. Sensitivity and engineered resistance of myeloid leukemia cells to BRD9 inhibition. Nat Chem Biol. 2016;12:672-9 pubmed publisher
Toivonen R, Kong L, Rasool O, Lund R, Lahesmaa R, Hänninen A. Activation of Plasmacytoid Dendritic Cells in Colon-Draining Lymph Nodes during Citrobacter rodentium Infection Involves Pathogen-Sensing and Inflammatory Pathways Distinct from Conventional Dendritic Cells. J Immunol. 2016;196:4750-9 pubmed publisher
Odeh A, Simecka J. Regulatory CD4+CD25+ T Cells Dampen Inflammatory Disease in Murine Mycoplasma Pneumonia and Promote IL-17 and IFN-γ Responses. PLoS ONE. 2016;11:e0155648 pubmed publisher
Cooley L, El Shikh M, Li W, Keim R, Zhang Z, Strauss J, et al. Impaired immunological synapse in sperm associated antigen 6 (SPAG6) deficient mice. Sci Rep. 2016;6:25840 pubmed publisher
Li Y, Takahashi Y, Fujii S, Zhou Y, Hong R, Suzuki A, et al. EAF2 mediates germinal centre B-cell apoptosis to suppress excessive immune responses and prevent autoimmunity. Nat Commun. 2016;7:10836 pubmed publisher
Saintamand A, Vincent Fabert C, Garot A, Rouaud P, Oruc Z, Magnone V, et al. Deciphering the importance of the palindromic architecture of the immunoglobulin heavy-chain 3' regulatory region. Nat Commun. 2016;7:10730 pubmed publisher
Nocera D, Roselli E, Araya P, Nuñez N, Lienenklaus S, Jablonska J, et al. In Vivo Visualizing the IFN-β Response Required for Tumor Growth Control in a Therapeutic Model of Polyadenylic-Polyuridylic Acid Administration. J Immunol. 2016;196:2860-9 pubmed publisher
Cunningham C, Champhekar A, Tullius M, Dillon B, Zhen A, de la Fuente J, et al. Type I and Type II Interferon Coordinately Regulate Suppressive Dendritic Cell Fate and Function during Viral Persistence. PLoS Pathog. 2016;12:e1005356 pubmed publisher
Ma Z, Li W, Yoshiya S, Xu Y, Hata M, El Darawish Y, et al. Augmentation of Immune Checkpoint Cancer Immunotherapy with IL18. Clin Cancer Res. 2016;22:2969-80 pubmed publisher
Cuenca M, Romero X, Sintes J, Terhorst C, Engel P. Targeting of Ly9 (CD229) Disrupts Marginal Zone and B1 B Cell Homeostasis and Antibody Responses. J Immunol. 2016;196:726-37 pubmed publisher
Morse M, Clark K, Cascalho M, Kahlenberg J. Caspase-1 is required for maintenance of marginal zone B cells in pristane-induced lupus. Lupus. 2016;25:81-7 pubmed publisher
Minamitani T, Yasui T, Ma Y, Zhou H, Okuzaki D, Tsai C, et al. Evasion of affinity-based selection in germinal centers by Epstein-Barr virus LMP2A. Proc Natl Acad Sci U S A. 2015;112:11612-7 pubmed publisher
Clark K, Reed T, Wolf S, Lowe L, Hodgin J, Kahlenberg J. Epidermal injury promotes nephritis flare in lupus-prone mice. J Autoimmun. 2015;65:38-48 pubmed publisher
Wu Y, Wu Y, Mo S, Hsiao H, He Y, Lai M. Cellular FLIP Inhibits Myeloid Cell Activation by Suppressing Selective Innate Signaling. J Immunol. 2015;195:2612-23 pubmed publisher
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product information
Antigen :
CD45R
Apps. Abbrev. :
Flow Cytometry
Cat # :
103211
Clone :
RA3-6B2
Item :
APC anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
25 μg
Price (USD) :
25 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cat (Feline)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA