product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
FITC anti-mouse/human CD45R/B220
catalog :
103206
quantity :
500 μg
price :
105 USD
clonality :
monoclonal
host :
rat
conjugate :
FITC
clone name :
RA3-6B2
reactivity :
human, mouse
application :
flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 112
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:400; loading ...; fig s1j
Bielecki P, Riesenfeld S, Hütter J, Torlai Triglia E, Kowalczyk M, Ricardo Gonzalez R, et al. Skin-resident innate lymphoid cells converge on a pathogenic effector state. Nature. 2021;592:128-132 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1h
Hammerich L, Marron T, Upadhyay R, Svensson Arvelund J, Dhainaut M, Hussein S, et al. Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination. Nat Med. 2019;25:814-824 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Meyer I, Goetzke C, Kespohl M, Sauter M, Heuser A, Eckstein V, et al. Silencing the CSF-1 Axis Using Nanoparticle Encapsulated siRNA Mitigates Viral and Autoimmune Myocarditis. Front Immunol. 2018;9:2303 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s1a
Tang H, Liang Y, Anders R, Taube J, Qiu X, Mulgaonkar A, et al. PD-L1 on host cells is essential for PD-L1 blockade-mediated tumor regression. J Clin Invest. 2018;128:580-588 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1b
Mildner A, Schönheit J, Giladi A, David E, Lara Astiaso D, Lorenzo Vivas E, et al. Genomic Characterization of Murine Monocytes Reveals C/EBP? Transcription Factor Dependence of Ly6C- Cells. Immunity. 2017;46:849-862.e7 pubmed publisher
  • flow cytometry; human; 1:100; loading ...
Lu X, Chen Q, Rong Y, Yang G, Li C, Xu N, et al. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells. Nat Commun. 2016;7:12719 pubmed publisher
  • flow cytometry; mouse
Leon Rico D, Fernández García M, Aldea M, Sánchez R, Peces Barba M, Martínez Palacio J, et al. Comparison of haematopoietic stem cell engraftment through the retro-orbital venous sinus and the lateral vein: alternative routes for bone marrow transplantation in mice. Lab Anim. 2015;49:132-41 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 6
  • flow cytometry; mouse; fig 4
Cao A, Yao S, Gong B, Nurieva R, Elson C, Cong Y. Interleukin (IL)-21 promotes intestinal IgA response to microbiota. Mucosal Immunol. 2015;8:1072-82 pubmed publisher
  • flow cytometry; mouse; fig 3
Chaimowitz N, Falanga Y, Ryan J, Conrad D. Fyn kinase is required for optimal humoral responses. PLoS ONE. 2013;8:e60640 pubmed publisher
Yang K, Han J, Asada M, Gill J, Park J, Sathe M, et al. Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. J Clin Invest. 2022;132: pubmed publisher
Johnson K, Soukup A, Bresnick E. GATA2 deficiency elevates interferon regulatory factor-8 to subvert a progenitor cell differentiation program. Blood Adv. 2022;6:1464-1473 pubmed publisher
Zheng Y, Chen Z, Zhou B, Chen S, Han L, Chen N, et al. PRMT5 Deficiency Enforces the Transcriptional and Epigenetic Programs of Klrg1+CD8+ Terminal Effector T Cells and Promotes Cancer Development. J Immunol. 2022;208:501-513 pubmed publisher
Ohyagi M, Nagata T, Ihara K, Yoshida Tanaka K, Nishi R, Miyata H, et al. DNA/RNA heteroduplex oligonucleotide technology for regulating lymphocytes in vivo. Nat Commun. 2021;12:7344 pubmed publisher
Tanaka Y, Nakao A, Miyake Y, Higashi Y, Tanigaki R, Kataoka T. Small Molecule Inhibitors Targeting Nuclear Factor κB Activation Markedly Reduce Expression of Interleukin-2, but Not Interferon-γ, Induced by Phorbol Esters and Calcium Ionophores. Int J Mol Sci. 2021;22: pubmed publisher
Nguyen H, Guevarra R, Magez S, Radwanska M. Single-cell transcriptome profiling and the use of AID deficient mice reveal that B cell activation combined with antibody class switch recombination and somatic hypermutation do not benefit the control of experimental trypanosomosis. PLoS Pathog. 2021;17:e1010026 pubmed publisher
Dong L, Chen C, Zhang Y, Guo P, Wang Z, Li J, et al. The loss of RNA N6-adenosine methyltransferase Mettl14 in tumor-associated macrophages promotes CD8+ T cell dysfunction and tumor growth. Cancer Cell. 2021;39:945-957.e10 pubmed publisher
Devito N, Sturdivant M, Thievanthiran B, Xiao C, Plebanek M, Salama A, et al. Pharmacological Wnt ligand inhibition overcomes key tumor-mediated resistance pathways to anti-PD-1 immunotherapy. Cell Rep. 2021;35:109071 pubmed publisher
Chen R, Okeyo Owuor T, Patel R, Casey E, Cluster A, Yang W, et al. Kmt2c mutations enhance HSC self-renewal capacity and convey a selective advantage after chemotherapy. Cell Rep. 2021;34:108751 pubmed publisher
Srivastava S, Furlan S, Jaeger Ruckstuhl C, Sarvothama M, Berger C, Smythe K, et al. Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade. Cancer Cell. 2021;39:193-208.e10 pubmed publisher
Younes A, Barsoumian H, Sezen D, Verma V, Patel R, Wasley M, et al. Addition of TLR9 agonist immunotherapy to radiation improves systemic antitumor activity. Transl Oncol. 2021;14:100983 pubmed publisher
Li K, Wang F, Yang Z, Zhang T, Yuan Y, Zhao C, et al. TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation. Nat Commun. 2020;11:6316 pubmed publisher
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Kobia F, Preusse K, Dai Q, Weaver N, Hass M, Chaturvedi P, et al. Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation. PLoS Biol. 2020;18:e3000850 pubmed publisher
Wong R, Belk J, Govero J, Uhrlaub J, Reinartz D, Zhao H, et al. Affinity-Restricted Memory B Cells Dominate Recall Responses to Heterologous Flaviviruses. Immunity. 2020;53:1078-1094.e7 pubmed publisher
Park H, Lim S, Hwang J, Zhang W, You S, Jin J. Cancer immunotherapy using a polysaccharide from Codium fragile in a murine model. Oncoimmunology. 2020;9:1772663 pubmed publisher
Yang W, Yu T, Huang X, Bilotta A, Xu L, Lu Y, et al. Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity. Nat Commun. 2020;11:4457 pubmed publisher
Jamali A, Hu K, Sendra V, Blanco T, Lopez M, Ortiz G, et al. Characterization of Resident Corneal Plasmacytoid Dendritic Cells and Their Pivotal Role in Herpes Simplex Keratitis. Cell Rep. 2020;32:108099 pubmed publisher
Li Y, Kong W, Yang W, Patel R, Casey E, Okeyo Owuor T, et al. Single-Cell Analysis of Neonatal HSC Ontogeny Reveals Gradual and Uncoordinated Transcriptional Reprogramming that Begins before Birth. Cell Stem Cell. 2020;27:732-747.e7 pubmed publisher
Li H, Limenitakis J, Greiff V, Yilmaz B, Schären O, Urbaniak C, et al. Mucosal or systemic microbiota exposures shape the B cell repertoire. Nature. 2020;584:274-278 pubmed publisher
Iwanami N, Takeshita K, Lawir D, Suetake I, Tajima S, Sikora K, et al. Epigenetic Protection of Vertebrate Lymphoid Progenitor Cells by Dnmt1. iScience. 2020;23:101260 pubmed publisher
Siamishi I, Iwanami N, Clapes T, Trompouki E, O Meara C, Boehm T. Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles. Cell Rep. 2020;31:107756 pubmed publisher
Zhang Y, Chen C, Deng C, Zhang C, Li N, Wang Z, et al. A novel rabies vaccine based on infectious propagating particles derived from hybrid VEEV-Rabies replicon. EBioMedicine. 2020;56:102819 pubmed publisher
Hewitson J, West K, James K, Rani G, Dey N, Romano A, et al. Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells. J Immunol. 2020;204:2949-2960 pubmed publisher
Morelli A, Sumpter T, Rojas Canales D, Bandyopadhyay M, Chen Z, Tkacheva O, et al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells. Cell Rep. 2020;30:3448-3465.e8 pubmed publisher
Zhang W, Xu L, Park H, Hwang J, Kwak M, Lee P, et al. Escherichia coli adhesion portion FimH functions as an adjuvant for cancer immunotherapy. Nat Commun. 2020;11:1187 pubmed publisher
Hirata S, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, et al. Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in mice. Allergy. 2020;75:1939-1955 pubmed publisher
Wu N, Cernysiov V, Davidson D, Song H, Tang J, Luo S, et al. Critical Role of Lipid Scramblase TMEM16F in Phosphatidylserine Exposure and Repair of Plasma Membrane after Pore Formation. Cell Rep. 2020;30:1129-1140.e5 pubmed publisher
Elahi S, Holling G, Stablewski A, Olejniczak S. Improved hematopoietic differentiation of mouse embryonic stem cells through manipulation of the RNA binding protein ARS2. Stem Cell Res. 2020;43:101710 pubmed publisher
Sanchez H, Moroney J, Gan H, Shen T, Im J, Li T, et al. B cell-intrinsic epigenetic modulation of antibody responses by dietary fiber-derived short-chain fatty acids. Nat Commun. 2020;11:60 pubmed publisher
Raju S, Verbaro D, Egawa T. PD-1 Signaling Promotes Control of Chronic Viral Infection by Restricting Type-I-Interferon-Mediated Tissue Damage. Cell Rep. 2019;29:2556-2564.e3 pubmed publisher
Garo L, Ajay A, Fujiwara M, Beynon V, Kuhn C, Gabriely G, et al. Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity. Cell Rep. 2019;28:3353-3366.e5 pubmed publisher
Tian D, Yang L, Wang S, Zhu Y, Shi W, Zhang C, et al. Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma. Nat Commun. 2019;10:4246 pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Jing Y, Kang D, Liu L, Huang H, Chen A, Yang L, et al. Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation. J Allergy Clin Immunol. 2019;: pubmed publisher
Qi X, Yun C, Sun L, Xia J, Wu Q, Wang Y, et al. Gut microbiota-bile acid-interleukin-22 axis orchestrates polycystic ovary syndrome. Nat Med. 2019;25:1225-1233 pubmed publisher
Mariani S, Li Z, Rice S, Krieg C, Fragkogianni S, Robinson M, et al. Pro-inflammatory Aorta-Associated Macrophages Are Involved in Embryonic Development of Hematopoietic Stem Cells. Immunity. 2019;: pubmed publisher
Nowlan B, Williams E, Doran M, Levesque J. CD27, CD201, FLT3, CD48, and CD150 cell surface staining identifies long-term mouse hematopoietic stem cells in immunodeficient non-obese diabetic severe combined immune deficient derived strains. Haematologica. 2019;: pubmed publisher
Srivastava S, Salter A, Liggitt D, Yechan Gunja S, Sarvothama M, Cooper K, et al. Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting. Cancer Cell. 2019;35:489-503.e8 pubmed publisher
Wang G, Zhang L, Zhao X, Gao S, Qu L, Yu H, et al. The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat Commun. 2019;10:1125 pubmed publisher
Roy K, Mitchell S, Liu Y, Ohta S, Lin Y, Metzig M, et al. A Regulatory Circuit Controlling the Dynamics of NFκB cRel Transitions B Cells from Proliferation to Plasma Cell Differentiation. Immunity. 2019;50:616-628.e6 pubmed publisher
Dey A, Yang W, Gegonne A, Nishiyama A, Pan R, Yagi R, et al. BRD4 directs hematopoietic stem cell development and modulates macrophage inflammatory responses. EMBO J. 2019;38: pubmed publisher
Wang B, Lim J, Kajikawa T, Li X, Vallance B, Moutsopoulos N, et al. Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis. Cell Rep. 2019;26:1614-1626.e5 pubmed publisher
Zanotti K, Maul R, Yang W, Gearhart P. DNA Breaks in Ig V Regions Are Predominantly Single Stranded and Are Generated by UNG and MSH6 DNA Repair Pathways. J Immunol. 2019;202:1573-1581 pubmed publisher
Zegarra Ruiz D, El Beidaq A, Iñiguez A, Lubrano Di Ricco M, Manfredo Vieira S, Ruff W, et al. A Diet-Sensitive Commensal Lactobacillus Strain Mediates TLR7-Dependent Systemic Autoimmunity. Cell Host Microbe. 2019;25:113-127.e6 pubmed publisher
Kim C, Lee C, Jung J, Ghosh A, Hasan S, Hwang S, et al. The Transcription Factor Ets1 Suppresses T Follicular Helper Type 2 Cell Differentiation to Halt the Onset of Systemic Lupus Erythematosus. Immunity. 2018;49:1034-1048.e8 pubmed publisher
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Katsumura K, Mehta C, Hewitt K, Soukup A, Fraga de Andrade I, Ranheim E, et al. Human leukemia mutations corrupt but do not abrogate GATA-2 function. Proc Natl Acad Sci U S A. 2018;115:E10109-E10118 pubmed publisher
Delgado Benito V, Rosen D, Wang Q, Gazumyan A, Pai J, Oliveira T, et al. The Chromatin Reader ZMYND8 Regulates Igh Enhancers to Promote Immunoglobulin Class Switch Recombination. Mol Cell. 2018;72:636-649.e8 pubmed publisher
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Golan K, Kumari A, Kollet O, Khatib Massalha E, Subramaniam M, Ferreira Z, et al. Daily Onset of Light and Darkness Differentially Controls Hematopoietic Stem Cell Differentiation and Maintenance. Cell Stem Cell. 2018;23:572-585.e7 pubmed publisher
Paul S, Peddayelachagiri B, Nagaraj S, Konduru B, Batra H. Protective and therapeutic efficacy study of divalent fusion protein rL7/L12-Omp25 against B. abortus 544 in presence of IFNγ. Appl Microbiol Biotechnol. 2018;102:8895-8907 pubmed publisher
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Wu Y, Wu Y, Mo S, Hsiao H, He Y, Lai M. Cellular FLIP Inhibits Myeloid Cell Activation by Suppressing Selective Innate Signaling. J Immunol. 2015;195:2612-23 pubmed publisher
Kim B, Sasaki Y, Egawa T. Restriction of Nonpermissive RUNX3 Protein Expression in T Lymphocytes by the Kozak Sequence. J Immunol. 2015;195:1517-23 pubmed publisher
Morrison V, Uotila L, Llort Asens M, Savinko T, Fagerholm S. Optimal T Cell Activation and B Cell Antibody Responses In Vivo Require the Interaction between Leukocyte Function-Associated Antigen-1 and Kindlin-3. J Immunol. 2015;195:105-15 pubmed publisher
Hayashi K, Sasai M, Iwasaki A. Toll-like receptor 9 trafficking and signaling for type I interferons requires PIKfyve activity. Int Immunol. 2015;27:435-45 pubmed publisher
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Hsieh Y, Fu C, Hsieh M. Helminth-induced interleukin-4 abrogates invariant natural killer T cell activation-associated clearance of bacterial infection. Infect Immun. 2014;82:2087-97 pubmed publisher
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Ota T, Doyle Cooper C, Cooper A, Doores K, Aoki Ota M, Le K, et al. B cells from knock-in mice expressing broadly neutralizing HIV antibody b12 carry an innocuous B cell receptor responsive to HIV vaccine candidates. J Immunol. 2013;191:3179-85 pubmed publisher
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product information
Antigen :
CD45R
Apps. Abbrev. :
Flow Cytometry
Cat # :
103206
Clone :
RA3-6B2
Item :
FITC anti-mouse/human CD45R/B220
Isotype :
Rat IgG2a, κ
Other Names :
B220
Size :
500 μg
Price (USD) :
105 USD
Reactivity :
Mouse, Human, Cross-Reactivity: Cat (Feline)
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
FITC
Immunogen :
Abelson murine leukemia virus-induced pre-B tumor cells
Application Notes :
Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro and in vivo modulation of B cell responses 2-4 , immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections 5,6 , and spatial biology (IBEX) 14,15 .
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA