product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC/Fire™ 750 anti-mouse CD45
catalog :
103153
quantity :
25 μg
price :
105 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
30-F11
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s5a
Liu Y, Du J, Liu X, Wang L, Han Y, Huang C, et al. MG149 inhibits histone acetyltransferase KAT8-mediated IL-33 acetylation to alleviate allergic airway inflammation and airway hyperresponsiveness. Signal Transduct Target Ther. 2021;6:321 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5e
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a, s2b
Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
  • flow cytometry; mouse; loading ...
Zheng W, Zhao D, Zhang H, Chinnasamy P, SIBINGA N, Pollard J. Induction of interferon signaling and allograft inflammatory factor 1 in macrophages in a mouse model of breast cancer metastases. Wellcome Open Res. 2021;6:52 pubmed publisher
  • flow cytometry; human; loading ...
Wong S, Lenzini S, Cooper M, Mooney D, Shin J. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Sci Adv. 2020;6:eaaw0158 pubmed publisher
  • flow cytometry; mouse; fig e2a
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...
Lopez M, Seyed Razavi Y, Jamali A, Harris D, Hamrah P. The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea. Invest Ophthalmol Vis Sci. 2018;59:5671-5681 pubmed publisher
  • flow cytometry; mouse; fig 2a
James K, Cosway E, LUCAS B, White A, Parnell S, Carvalho Gaspar M, et al. Endothelial cells act as gatekeepers for LTβR-dependent thymocyte emigration. J Exp Med. 2018;215:2984-2993 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3f
Pan D, Kobayashi A, Jiang P, Ferrari de Andrade L, Tay R, Luoma A, et al. A major chromatin regulator determines resistance of tumor cells to T cell-mediated killing. Science. 2018;359:770-775 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig 2g
Lang S, Harre U, Purohit P, Dietel K, Kienhöfer D, Hahn J, et al. Neurodegeneration Enhances the Development of Arthritis. J Immunol. 2017;198:2394-2402 pubmed publisher
  • flow cytometry; mouse
Mangum L, CROW J, Stokes J, HOWELL G, Ross M, Pruett S, et al. Exposure to p,p'-DDE Alters Macrophage Reactivity and Increases Macrophage Numbers in Adipose Stromal Vascular Fraction. Toxicol Sci. 2016;150:169-77 pubmed publisher
  • flow cytometry; mouse
Maruyama A, Shime H, Takeda Y, Azuma M, Matsumoto M, Seya T. Pam2 lipopeptides systemically increase myeloid-derived suppressor cells through TLR2 signaling. Biochem Biophys Res Commun. 2015;457:445-50 pubmed publisher
Jacoby E, Ben Yakir Blumkin M, Blumenfeld Kan S, Brody Y, Meir A, Melamed Book N, et al. Mitochondrial augmentation of CD34+ cells from healthy donors and patients with mitochondrial DNA disorders confers functional benefit. NPJ Regen Med. 2021;6:58 pubmed publisher
Hildebrand K, Singla A, McNeil R, Marritt K, Hildebrand K, Zemp F, et al. The KrasG12D;Trp53fl/fl murine model of undifferentiated pleomorphic sarcoma is macrophage dense, lymphocyte poor, and resistant to immune checkpoint blockade. PLoS ONE. 2021;16:e0253864 pubmed publisher
Galeano Niño J, Pageon S, Tay S, Colakoglu F, Kempe D, Hywood J, et al. Cytotoxic T cells swarm by homotypic chemokine signalling. elife. 2020;9: pubmed publisher
Parker K, Migliorini D, Perkey E, Yost K, Bhaduri A, Bagga P, et al. Single-Cell Analyses Identify Brain Mural Cells Expressing CD19 as Potential Off-Tumor Targets for CAR-T Immunotherapies. Cell. 2020;183:126-142.e17 pubmed publisher
Tran S, Baba I, Poupel L, Dussaud S, Moreau M, Gélineau A, et al. Impaired Kupffer Cell Self-Renewal Alters the Liver Response to Lipid Overload during Non-alcoholic Steatohepatitis. Immunity. 2020;53:627-640.e5 pubmed publisher
Sagnella S, Yang L, Stubbs G, Boslem E, Martino Echarri E, Smolarczyk K, et al. Cyto-Immuno-Therapy for Cancer: A Pathway Elicited by Tumor-Targeted, Cytotoxic Drug-Packaged Bacterially Derived Nanocells. Cancer Cell. 2020;37:354-370.e7 pubmed publisher
Bottek J, Soun C, Lill J, Dixit A, Thiebes S, Beerlage A, et al. Spatial proteomics revealed a CX3CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder. Mucosal Immunol. 2020;13:702-714 pubmed publisher
Schaffert A, Hanić M, Novokmet M, Zaytseva O, Krištić J, Lux A, et al. Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo. Front Immunol. 2019;10:3024 pubmed publisher
Levesque S, Le Naour J, Pietrocola F, Paillet J, Kremer M, Castoldi F, et al. A synergistic triad of chemotherapy, immune checkpoint inhibitors, and caloric restriction mimetics eradicates tumors in mice. Oncoimmunology. 2019;8:e1657375 pubmed publisher
Fulham M, Ratna A, Gerstein R, Kurt Jones E, Mandrekar P. Alcohol-induced adipose tissue macrophage phenotypic switching is independent of myeloid Toll-like receptor 4 expression. Am J Physiol Cell Physiol. 2019;317:C687-C700 pubmed publisher
Sharba S, Navabi N, Padra M, Persson J, Quintana Hayashi M, Gustafsson J, et al. Interleukin 4 induces rapid mucin transport, increases mucus thickness and quality and decreases colitis and Citrobacter rodentium in contact with epithelial cells. Virulence. 2019;10:97-117 pubmed publisher
Lai S, Sheng J, Gupta P, Renia L, Duan K, Zolezzi F, et al. Organ-Specific Fate, Recruitment, and Refilling Dynamics of Tissue-Resident Macrophages during Blood-Stage Malaria. Cell Rep. 2018;25:3099-3109.e3 pubmed publisher
Li Z, Liu S, Xu J, Zhang X, Han D, Liu J, et al. Adult Connective Tissue-Resident Mast Cells Originate from Late Erythro-Myeloid Progenitors. Immunity. 2018;49:640-653.e5 pubmed publisher
Pelletier A, Guimont Desrochers F, Ashton M, Brodnicki T, Lesage S. The size of the plasmacytoid dendritic cell compartment is a multigenic trait dominated by a locus on mouse chromosome 7. J Immunol. 2012;188:5561-70 pubmed publisher
Liu F, Kang I, Park C, Chang L, Wang W, Lee D, et al. ER71 specifies Flk-1+ hemangiogenic mesoderm by inhibiting cardiac mesoderm and Wnt signaling. Blood. 2012;119:3295-305 pubmed publisher
Podd B, Thoits J, Whitley N, Cheng H, Kudla K, Taniguchi H, et al. T cells in cryptopatch aggregates share TCR gamma variable region junctional sequences with gamma delta T cells in the small intestinal epithelium of mice. J Immunol. 2006;176:6532-42 pubmed
Tsuboi S, Fukuda M. Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs humoral immune responses in transgenic mice. J Biol Chem. 1998;273:30680-7 pubmed
product information
Antigen :
CD45
Apps. Abbrev. :
Flow Cytometry
Cat # :
103153
Clone :
30-F11
Item :
APC/Fire™ 750 anti-mouse CD45
Isotype :
Rat IgG2b, κ
Other Names :
T200, Ly-5, LCA
Size :
25 μg
Price (USD) :
105 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC_F750
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. Additional reported applications (for relevant formats) include: immunoprecipitation 3 , complement-dependent cytotoxicity 1,5 , immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections) 4,6 , Western blotting 7 , and spatial biology (IBEX) 10,11 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA