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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse CD45
catalog :
103134
quantity :
50 μg
price :
259 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
30-F11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 63
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig 5h
Tulotta C, Lefley D, Moore C, Amariutei A, Spicer Hadlington A, Quayle L, et al. IL-1B drives opposing responses in primary tumours and bone metastases; harnessing combination therapies to improve outcome in breast cancer. NPJ Breast Cancer. 2021;7:95 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 2b
Celorrio M, Abellanas M, Rhodes J, Goodwin V, Moritz J, Vadivelu S, et al. Gut microbial dysbiosis after traumatic brain injury modulates the immune response and impairs neurogenesis. Acta Neuropathol Commun. 2021;9:40 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a, 5b
Nagai J, Balestrieri B, Fanning L, Kyin T, Cirka H, Lin J, et al. P2Y6 signaling in alveolar macrophages prevents leukotriene-dependent type 2 allergic lung inflammation. J Clin Invest. 2019;129:5169-5186 pubmed publisher
  • flow cytometry; mouse; 1:150; loading ...; fig 5c
Rosenzweig N, Dvir Szternfeld R, Tsitsou Kampeli A, Keren Shaul H, Ben Yehuda H, Weill Raynal P, et al. PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse model. Nat Commun. 2019;10:465 pubmed publisher
  • flow cytometry; mouse; fig 6a
Barrow A, Edeling M, Trifonov V, Luo J, Goyal P, Bohl B, et al. Natural Killer Cells Control Tumor Growth by Sensing a Growth Factor. Cell. 2018;172:534-548.e19 pubmed publisher
  • flow cytometry; mouse; fig s12
Calon A, Lonardo E, Berenguer Llergo A, Espinet E, Hernando Momblona X, Iglesias M, et al. Stromal gene expression defines poor-prognosis subtypes in colorectal cancer. Nat Genet. 2015;47:320-9 pubmed publisher
  • flow cytometry; mouse; fig s12
Zimmermann M, Aguilera F, Castellucci M, Rossato M, Costa S, Lunardi C, et al. Chromatin remodelling and autocrine TNFα are required for optimal interleukin-6 expression in activated human neutrophils. Nat Commun. 2015;6:6061 pubmed publisher
  • flow cytometry; mouse
Makarem M, Kannan N, Nguyen L, Knapp D, Balani S, Prater M, et al. Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells. PLoS Biol. 2013;11:e1001630 pubmed publisher
Mathur S, Wang J, Seehus C, Poirier F, Crosson T, Hsieh Y, et al. Nociceptor neurons promote IgE class switch in B cells. JCI Insight. 2021;6: pubmed publisher
Carbone C, Piro G, Agostini A, Delfino P, De Sanctis F, Nasca V, et al. Intratumoral injection of TLR9 agonist promotes an immunopermissive microenvironment transition and causes cooperative antitumor activity in combination with anti-PD1 in pancreatic cancer. J Immunother Cancer. 2021;9: pubmed publisher
Zheng H, Zhang Y, He J, Yang Z, Zhang R, Li L, et al. Hydroxychloroquine Inhibits Macrophage Activation and Attenuates Renal Fibrosis After Ischemia-Reperfusion Injury. Front Immunol. 2021;12:645100 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Zheng H, Zhang Y, Li L, Zhang R, Luo Z, Yang Z, et al. Depletion of Toll-Like Receptor-9 Attenuates Renal Tubulointerstitial Fibrosis After Ischemia-Reperfusion Injury. Front Cell Dev Biol. 2021;9:641527 pubmed publisher
Dubrot J, Lane Reticker S, Kessler E, Ayer A, Mishra G, Wolfe C, et al. In vivo screens using a selective CRISPR antigen removal lentiviral vector system reveal immune dependencies in renal cell carcinoma. Immunity. 2021;: pubmed publisher
Nishimura T, Suchy F, Bhadury J, Igarashi K, Charlesworth C, Nakauchi H. Generation of Functional Organs Using a Cell-Competitive Niche in Intra- and Inter-species Rodent Chimeras. Cell Stem Cell. 2021;28:141-149.e3 pubmed publisher
Engler A, Ysasi A, Pihl R, Villacorta Martin C, Heston H, Richardson H, et al. Airway-Associated Macrophages in Homeostasis and Repair. Cell Rep. 2020;33:108553 pubmed publisher
Li J, Zhang L, Zheng Y, Shao R, Liang Q, Yu W, et al. BAD inactivation exacerbates rheumatoid arthritis pathology by promoting survival of sublining macrophages. elife. 2020;9: pubmed publisher
Guldner I, Wang Q, Yang L, Golomb S, Zhao Z, Lopez J, et al. CNS-Native Myeloid Cells Drive Immune Suppression in the Brain Metastatic Niche through Cxcl10. Cell. 2020;183:1234-1248.e25 pubmed publisher
Kobayashi H, Takubo K. Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells. STAR Protoc. 2020;1:100078 pubmed publisher
Goh W, Scheer S, Jackson J, Hediyeh Zadeh S, Delconte R, Schuster I, et al. Hhex Directly Represses BIM-Dependent Apoptosis to Promote NK Cell Development and Maintenance. Cell Rep. 2020;33:108285 pubmed publisher
Omori S, Wang T, Johmura Y, Kanai T, Nakano Y, Kido T, et al. Generation of a p16 Reporter Mouse and Its Use to Characterize and Target p16high Cells In Vivo. Cell Metab. 2020;32:814-828.e6 pubmed publisher
Dinh H, Eggert T, Meyer M, Zhu Y, Olingy C, Llewellyn R, et al. Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow. Immunity. 2020;53:319-334.e6 pubmed publisher
Lee H, Lee Y, Harenda Q, Pietrzak S, Oktay H, Schreiber S, et al. Beta Cell Dedifferentiation Induced by IRE1α Deletion Prevents Type 1 Diabetes. Cell Metab. 2020;31:822-836.e5 pubmed publisher
Süß P, Hoffmann A, Rothe T, Ouyang Z, Baum W, Staszewski O, et al. Chronic Peripheral Inflammation Causes a Region-Specific Myeloid Response in the Central Nervous System. Cell Rep. 2020;30:4082-4095.e6 pubmed publisher
Hu B, Lv X, Chen H, Xue P, Gao B, Wang X, et al. Sensory nerves regulate mesenchymal stromal cell lineage commitment by tuning sympathetic tones. J Clin Invest. 2020;130:3483-3498 pubmed publisher
Saika A, Nagatake T, Kishino S, Park S, Honda T, Matsumoto N, et al. 17(S),18(R)-epoxyeicosatetraenoic acid generated by cytochrome P450 BM-3 from Bacillus megaterium inhibits the development of contact hypersensitivity via G-protein-coupled receptor 40-mediated neutrophil suppression. FASEB Bioadv. 2020;2:59-71 pubmed publisher
Hirata S, Nagatake T, Sawane K, Hosomi K, Honda T, Ono S, et al. Maternal ω3 docosapentaenoic acid inhibits infant allergic dermatitis through TRAIL-expressing plasmacytoid dendritic cells in mice. Allergy. 2020;75:1939-1955 pubmed publisher
Naito H, Wakabayashi T, Ishida M, Gil C, Iba T, Rahmawati F, et al. Isolation of tissue-resident vascular endothelial stem cells from mouse liver. Nat Protoc. 2020;15:1066-1081 pubmed publisher
Bersini S, Lytle N, Schulte R, Huang L, Wahl G, Hetzer M. Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling. Life Sci Alliance. 2020;3: pubmed publisher
Dobson H, Dias L, Kohn E, Fites S, Wiesner D, Dileepan T, et al. Antigen discovery unveils resident memory and migratory cell roles in antifungal resistance. Mucosal Immunol. 2020;13:518-529 pubmed publisher
Hagan A, Zhang B, Ornitz D. Identification of a FGF18-expressing alveolar myofibroblast that is developmentally cleared during alveologenesis. Development. 2020;147: pubmed publisher
Katara G, Kulshrestha A, Schneiderman S, Riehl V, Ibrahim S, Beaman K. Interleukin-22 promotes development of malignant lesions in a mouse model of spontaneous breast cancer. Mol Oncol. 2020;14:211-224 pubmed publisher
Mao W, Ghasemzadeh A, Freeman Z, Obradovic A, Chaimowitz M, Nirschl T, et al. Immunogenicity of prostate cancer is augmented by BET bromodomain inhibition. J Immunother Cancer. 2019;7:277 pubmed publisher
Shmeleva E, Smith G, Ferguson B. Enhanced Efficacy of Vaccination With Vaccinia Virus in Old vs. Young Mice. Front Immunol. 2019;10:1780 pubmed publisher
Saleh M, Frisbee A, Leslie J, Buonomo E, Cowardin C, Ma J, et al. Colitis-Induced Th17 Cells Increase the Risk for Severe Subsequent Clostridium difficile Infection. Cell Host Microbe. 2019;25:756-765.e5 pubmed publisher
Parlet C, Kavanaugh J, Crosby H, Raja H, El Elimat T, Todd D, et al. Apicidin Attenuates MRSA Virulence through Quorum-Sensing Inhibition and Enhanced Host Defense. Cell Rep. 2019;27:187-198.e6 pubmed publisher
Dahlgren M, Jones S, Cautivo K, Dubinin A, Ortiz Carpena J, Farhat S, et al. Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches. Immunity. 2019;50:707-722.e6 pubmed publisher
Zwick M, Ulas T, Cho Y, Ried C, Grosse L, Simon C, et al. Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8+ Dendritic Cells. Front Immunol. 2019;10:222 pubmed publisher
Kurelac I, Iommarini L, Vatrinet R, Amato L, De Luise M, Leone G, et al. Inducing cancer indolence by targeting mitochondrial Complex I is potentiated by blocking macrophage-mediated adaptive responses. Nat Commun. 2019;10:903 pubmed publisher
Kobayashi T, Voisin B, Kim D, Kennedy E, Jo J, Shih H, et al. Homeostatic Control of Sebaceous Glands by Innate Lymphoid Cells Regulates Commensal Bacteria Equilibrium. Cell. 2019;176:982-997.e16 pubmed publisher
Li Q, Lan X, Han X, Wang J. Expression of Tmem119/Sall1 and Ccr2/CD69 in FACS-Sorted Microglia- and Monocyte/Macrophage-Enriched Cell Populations After Intracerebral Hemorrhage. Front Cell Neurosci. 2018;12:520 pubmed publisher
Karlen S, Miller E, Wang X, Levine E, Zawadzki R, Burns M. Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration. J Neuroinflammation. 2018;15:344 pubmed publisher
Durré T, Morfoisse F, Erpicum C, Ebroin M, Blacher S, García Caballero M, et al. uPARAP/Endo180 receptor is a gatekeeper of VEGFR-2/VEGFR-3 heterodimerisation during pathological lymphangiogenesis. Nat Commun. 2018;9:5178 pubmed publisher
Kramann R, Machado F, Wu H, Kusaba T, Hoeft K, Schneider R, et al. Parabiosis and single-cell RNA sequencing reveal a limited contribution of monocytes to myofibroblasts in kidney fibrosis. JCI Insight. 2018;3: pubmed publisher
Wakabayashi T, Naito H, Suehiro J, Lin Y, Kawaji H, Iba T, et al. CD157 Marks Tissue-Resident Endothelial Stem Cells with Homeostatic and Regenerative Properties. Cell Stem Cell. 2018;22:384-397.e6 pubmed publisher
Mamedov M, Scholzen A, Nair R, Cumnock K, Kenkel J, Oliveira J, et al. A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence. Immunity. 2018;48:350-363.e7 pubmed publisher
Crncec I, Modak M, Gordziel C, Svinka J, Scharf I, Moritsch S, et al. STAT1 is a sex-specific tumor suppressor in colitis-associated colorectal cancer. Mol Oncol. 2018;12:514-528 pubmed publisher
Song W, Joshita S, Zhou Y, Ulland T, Gilfillan S, Colonna M. Humanized TREM2 mice reveal microglia-intrinsic and -extrinsic effects of R47H polymorphism. J Exp Med. 2018;215:745-760 pubmed publisher
Nagatake T, Shiogama Y, Inoue A, Kikuta J, Honda T, Tiwari P, et al. The 17,18-epoxyeicosatetraenoic acid-G protein-coupled receptor 40 axis ameliorates contact hypersensitivity by inhibiting neutrophil mobility in mice and cynomolgus macaques. J Allergy Clin Immunol. 2018;142:470-484.e12 pubmed publisher
Glodde N, Bald T, van den Boorn Konijnenberg D, Nakamura K, O Donnell J, Szczepanski S, et al. Reactive Neutrophil Responses Dependent on the Receptor Tyrosine Kinase c-MET Limit Cancer Immunotherapy. Immunity. 2017;47:789-802.e9 pubmed publisher
Deczkowska A, Matcovitch Natan O, Tsitsou Kampeli A, Ben Hamo S, Dvir Szternfeld R, Spinrad A, et al. Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. Nat Commun. 2017;8:717 pubmed publisher
Ulland T, Song W, Huang S, ULRICH J, Sergushichev A, Beatty W, et al. TREM2 Maintains Microglial Metabolic Fitness in Alzheimer's Disease. Cell. 2017;170:649-663.e13 pubmed publisher
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product information
Antigen :
CD45
Apps. Abbrev. :
Flow Cytometry, SB
Cat # :
103134
Clone :
30-F11
Item :
Brilliant Violet 421™ anti-mouse CD45
Isotype :
Rat IgG2b, κ
Other Names :
T200, Ly-5, LCA
Size :
50 μg
Price (USD) :
259 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. Additional reported applications (for relevant formats) include: immunoprecipitation 3 , complement-dependent cytotoxicity 1,5 , immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections) 4,6 , Western blotting 7 , and spatial biology (IBEX) 10,11 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA