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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PerCP anti-mouse CD45
catalog :
103129
quantity :
25 μg
price :
99 USD
clonality :
monoclonal
host :
rat
conjugate :
PerCP
clone name :
30-F11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 69
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; loading ...; fig s10
Beckmann D, Römer Hillmann A, Krause A, Hansen U, Wehmeyer C, Intemann J, et al. Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis. Nat Commun. 2021;12:3624 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Demandt J, van Kuijk K, Theelen T, Marsch E, Heffron S, Fisher E, et al. Whole-Body Prolyl Hydroxylase Domain (PHD) 3 Deficiency Increased Plasma Lipids and Hematocrit Without Impacting Plaque Size in Low-Density Lipoprotein Receptor Knockout Mice. Front Cell Dev Biol. 2021;9:664258 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1s1a
Wells K, Miller C, Gschwind A, Wei W, Phipps J, Anderson M, et al. Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:100; fig s1a, s2a
Azcutia V, Kelm M, Luissint A, Boerner K, Flemming S, Quirós M, et al. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo. Mucosal Immunol. 2020;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3b
Cornelissen L, Blanas A, van der Horst J, Kruijssen L, Zaal A, O Toole T, et al. Disruption of sialic acid metabolism drives tumor growth by augmenting CD8+ T cell apoptosis. Int J Cancer. 2019;144:2290-2302 pubmed publisher
  • flow cytometry; mouse; fig s3
Hrdinka M, Sudan K, Just S, Drobek A, Stepanek O, Schluter D, et al. Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice. PLoS ONE. 2016;11:e0162863 pubmed publisher
  • flow cytometry; mouse; fig 1a
Engelmann R, Biemelt A, Cordshagen A, Johl A, Kuthning D, Müller Hilke B. The Prerequisites for Central Tolerance Induction against Citrullinated Proteins in the Mouse. PLoS ONE. 2016;11:e0158773 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1
Woods S, Waite A, O Dea K, Halford P, Takata M, Wilson M. Kinetic profiling of in vivo lung cellular inflammatory responses to mechanical ventilation. Am J Physiol Lung Cell Mol Physiol. 2015;308:L912-21 pubmed publisher
  • flow cytometry; mouse
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Zhou J, Yang Q, Lu L, Tuo Z, Shou Z, Cheng J. PLK1 Inhibition Induces Immunogenic Cell Death and Enhances Immunity against NSCLC. Int J Med Sci. 2021;18:3516-3525 pubmed publisher
Wang X, Maxwell K, Wang K, Bowers D, Flanders J, Liu W, et al. A nanofibrous encapsulation device for safe delivery of insulin-producing cells to treat type 1 diabetes. Sci Transl Med. 2021;13: pubmed publisher
He Y, Fu L, Li Y, Wang W, Gong M, Zhang J, et al. Gut microbial metabolites facilitate anticancer therapy efficacy by modulating cytotoxic CD8+ T cell immunity. Cell Metab. 2021;33:988-1000.e7 pubmed publisher
Taddio M, Castro Jaramillo C, Runge P, Blanc A, Keller C, Talip Z, et al. In Vivo Imaging of Local Inflammation: Monitoring LPS-Induced CD80/CD86 Upregulation by PET. Mol Imaging Biol. 2021;23:196-207 pubmed publisher
Wang X, Wilhelm J, Li W, Li S, Wang Z, Huang G, et al. Polycarbonate-based ultra-pH sensitive nanoparticles improve therapeutic window. Nat Commun. 2020;11:5828 pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Kerkhofs D, Van Hagen B, Milanova I, Schell K, van Essen H, Wijnands E, et al. Pharmacological depletion of microglia and perivascular macrophages prevents Vascular Cognitive Impairment in Ang II-induced hypertension. Theranostics. 2020;10:9512-9527 pubmed publisher
Yamamuro T, Kawabata T, Fukuhara A, Saita S, Nakamura S, Takeshita H, et al. Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy. Nat Commun. 2020;11:4150 pubmed publisher
Choi E, Lee M, Song J, Kim K, Lee J, Yang J, et al. Fas mutation reduces obesity by increasing IL-4 and IL-10 expression and promoting white adipose tissue browning. Sci Rep. 2020;10:12001 pubmed publisher
Peng L, Wang Y, Fei S, Wei C, Tong F, Wu G, et al. The effect of combining Endostar with radiotherapy on blood vessels, tumor-associated macrophages, and T cells in brain metastases of Lewis lung cancer. Transl Lung Cancer Res. 2020;9:745-760 pubmed publisher
Lin H, Wang Q, Liu L, Chen Z, Das R, Zhao Y, et al. Colonization of Mice With Amoxicillin-Associated Klebsiella variicola Drives Inflammation via Th1 Induction and Treg Inhibition. Front Microbiol. 2020;11:1256 pubmed publisher
Gomzikova M, Kletukhina S, Kurbangaleeva S, Neustroeva O, Vasileva O, Garanina E, et al. Mesenchymal Stem Cell Derived Biocompatible Membrane Vesicles Demonstrate Immunomodulatory Activity Inhibiting Activation and proliferation of Human Mononuclear Cells. Pharmaceutics. 2020;12: pubmed publisher
Liu Y, Tang B, Wang F, Tang L, Lei Y, Luo Y, et al. Parthenolide ameliorates colon inflammation through regulating Treg/Th17 balance in a gut microbiota-dependent manner. Theranostics. 2020;10:5225-5241 pubmed publisher
Haratani K, Yonesaka K, Takamura S, Maenishi O, Kato R, Takegawa N, et al. U3-1402 sensitizes HER3-expressing tumors to PD-1 blockade by immune activation. J Clin Invest. 2020;130:374-388 pubmed publisher
Wang F, Sun F, Luo J, Yue T, Chen L, Zhou H, et al. Loss of ubiquitin-conjugating enzyme E2 (Ubc9) in macrophages exacerbates multiple low-dose streptozotocin-induced diabetes by attenuating M2 macrophage polarization. Cell Death Dis. 2019;10:892 pubmed publisher
Zhao Y, Lee C, Lin C, Gassen R, Xu X, Huang Z, et al. PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways. Immunity. 2019;51:1059-1073.e9 pubmed publisher
She L, Alanazi H, Yan L, Zou Y, Sun Y, Dube P, et al. Immune Sensing of Aeroallergen-Associated Double-Stranded RNA Triggers an IFN Response and Modulates Type 2 Lung Inflammation. J Immunol. 2019;203:2520-2531 pubmed publisher
Westrich J, Vermeer D, Silva A, Bonney S, Berger J, Cicchini L, et al. CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8+ T-cell responses by upregulating MHC-I expression. Oncogene. 2019;: pubmed publisher
Xiong X, Kuang H, Ansari S, Liu T, Gong J, Wang S, et al. Landscape of Intercellular Crosstalk in Healthy and NASH Liver Revealed by Single-Cell Secretome Gene Analysis. Mol Cell. 2019;75:644-660.e5 pubmed publisher
Kumaran Satyanarayanan S, El Kebir D, Soboh S, Butenko S, Sekheri M, Saadi J, et al. IFN-β is a macrophage-derived effector cytokine facilitating the resolution of bacterial inflammation. Nat Commun. 2019;10:3471 pubmed publisher
Yang S, Wei J, Cui Y, Park G, Shah P, Deng Y, et al. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. Nat Commun. 2019;10:2782 pubmed publisher
Nguyen C, Tartar D, Bagood M, So M, Nguyen A, Gallegos A, et al. Topical Fluoxetine as a Novel Therapeutic That Improves Wound Healing in Diabetic Mice. Diabetes. 2019;68:1499-1507 pubmed publisher
Wang B, Lim J, Kajikawa T, Li X, Vallance B, Moutsopoulos N, et al. Macrophage β2-Integrins Regulate IL-22 by ILC3s and Protect from Lethal Citrobacter rodentium-Induced Colitis. Cell Rep. 2019;26:1614-1626.e5 pubmed publisher
Dmitrieva Posocco O, Dzutsev A, Posocco D, Hou V, Yuan W, Thovarai V, et al. Cell-Type-Specific Responses to Interleukin-1 Control Microbial Invasion and Tumor-Elicited Inflammation in Colorectal Cancer. Immunity. 2019;50:166-180.e7 pubmed publisher
Chen R, Miao Y, Hu Z. Dynamic Nestin expression during hair follicle maturation and the normal hair cycle. Mol Med Rep. 2019;19:549-554 pubmed publisher
Fatkhullina A, Peshkova I, Dzutsev A, Aghayev T, McCulloch J, Thovarai V, et al. An Interleukin-23-Interleukin-22 Axis Regulates Intestinal Microbial Homeostasis to Protect from Diet-Induced Atherosclerosis. Immunity. 2018;49:943-957.e9 pubmed publisher
Yu B, Huo L, Liu Y, Deng P, Szymanski J, Li J, et al. PGC-1α Controls Skeletal Stem Cell Fate and Bone-Fat Balance in Osteoporosis and Skeletal Aging by Inducing TAZ. Cell Stem Cell. 2018;23:193-209.e5 pubmed publisher
Zukauskas A, Mrsny R, Cortés Barrantes P, Turner J, Leong J, McCormick B. Transporters MRP1 and MRP2 Regulate Opposing Inflammatory Signals To Control Transepithelial Neutrophil Migration during Streptococcus pneumoniae Lung Infection. mSphere. 2018;3: pubmed publisher
Wang X, Chu Q, Jiang X, Yu Y, Wang L, Cui Y, et al. Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway. Cell Death Dis. 2018;9:578 pubmed publisher
Rangaraju S, Dammer E, Raza S, Rathakrishnan P, Xiao H, Gao T, et al. Identification and therapeutic modulation of a pro-inflammatory subset of disease-associated-microglia in Alzheimer's disease. Mol Neurodegener. 2018;13:24 pubmed publisher
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Peñaloza H, Salazar Echegarai F, Bueno S. Interleukin 10 modulation of neutrophil subsets infiltrating lungs during Streptococcus pneumoniae infection. Biochem Biophys Rep. 2018;13:12-16 pubmed publisher
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product information
Antigen :
CD45
Apps. Abbrev. :
Flow Cytometry
Cat # :
103129
Clone :
30-F11
Item :
PerCP anti-mouse CD45
Isotype :
Rat IgG2b, κ
Other Names :
T200, Ly-5, LCA
Size :
25 μg
Price (USD) :
99 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PERCP
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45. Additional reported applications (for relevant formats) include: immunoprecipitation 3 , complement-dependent cytotoxicity 1,5 , immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections) 4,6 , Western blotting 7 , and spatial biology (IBEX) 10,11 . The Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) is recommended for functional assays (Cat. No. 103163 and 103164).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA