product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse CD45
catalog :
103126
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
30-F11
reactivity :
mouse
application :
flow cytometry
more info or order :
citations: 140
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:100; fig 3c, 4c
Taniguchi H, Caeser R, Chavan S, Zhan Y, Chow A, Manoj P, et al. WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell Rep. 2022;39:110814 pubmed publisher
  • flow cytometry; mouse; fig s12b
Li H, Liu Z, Liu L, Zhang H, Han C, Girard L, et al. AXL targeting restores PD-1 blockade sensitivity of STK11/LKB1 mutant NSCLC through expansion of TCF1+ CD8 T cells. Cell Rep Med. 2022;3:100554 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 4a, s3a
Wutschka J, Kast B, Sator Schmitt M, Appak Baskoy S, Hess J, Sinn H, et al. JUNB suppresses distant metastasis by influencing the initial metastatic stage. Clin Exp Metastasis. 2021;38:411-423 pubmed publisher
  • flow cytometry; mouse; loading ...
Hering L, Katkeviciute E, Schwarzfischer M, Niechcial A, Riggs J, Wawrzyniak M, et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a
Chavkin N, Sano S, Wang Y, Oshima K, Ogawa H, Horitani K, et al. The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation. J Am Heart Assoc. 2021;10:e019904 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s2c
Laine A, Nagelli S, Farrington C, Butt U, Cvrljevic A, Vainonen J, et al. CIP2A Interacts with TopBP1 and Drives Basal-Like Breast Cancer Tumorigenesis. Cancer Res. 2021;81:4319-4331 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig s1d
Souza C, Ketelut Carneiro N, Milanezi C, Faccioli L, Gardinassi L, Silva J. NLRC4 inhibits NLRP3 inflammasome and abrogates effective antifungal CD8+ T cell responses. iScience. 2021;24:102548 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Kastenschmidt J, Coulis G, Farahat P, Pham P, Rios R, Cristal T, et al. A stromal progenitor and ILC2 niche promotes muscle eosinophilia and fibrosis-associated gene expression. Cell Rep. 2021;35:108997 pubmed publisher
  • flow cytometry; mouse; loading ...
Zhang Y, Liu J, Wang X, Zhang J, Xie C. Extracellular vesicle-encapsulated microRNA-23a from dorsal root ganglia neurons binds to A20 and promotes inflammatory macrophage polarization following peripheral nerve injury. Aging (Albany NY). 2021;13:6752-6764 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...
Kurashima Y, Kigoshi T, Murasaki S, Arai F, Shimada K, Seki N, et al. Pancreatic glycoprotein 2 is a first line of defense for mucosal protection in intestinal inflammation. Nat Commun. 2021;12:1067 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 5a
Gurley J, Gmyrek G, McClellan M, Hargis E, Hauck S, Dozmorov M, et al. Neuroretinal-Derived Caveolin-1 Promotes Endotoxin-Induced Inflammation in the Murine Retina. Invest Ophthalmol Vis Sci. 2020;61:19 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...
Vacca F, Chauch C, Jamwal A, Hinchy E, Heieis G, Webster H, et al. A helminth-derived suppressor of ST2 blocks allergic responses. elife. 2020;9: pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig s6c
Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c, 2c, s7f
Komuczki J, Tuzlak S, Friebel E, Hartwig T, Spath S, Rosenstiel P, et al. Fate-Mapping of GM-CSF Expression Identifies a Discrete Subset of Inflammation-Driving T Helper Cells Regulated by Cytokines IL-23 and IL-1β. Immunity. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Quenum Zangbede F, Chauhan A, Sharma J, Mishra B. Galectin-3 in M2 Macrophages Plays a Protective Role in Resolution of Neuropathology in Brain Parasitic Infection by Regulating Neutrophil Turnover. J Neurosci. 2018;38:6737-6750 pubmed publisher
  • flow cytometry; mouse; loading ...; tbl s1
Kraakman M, Lee M, Al Sharea A, Dragoljevic D, Barrett T, Montenont E, et al. Neutrophil-derived S100 calcium-binding proteins A8/A9 promote reticulated thrombocytosis and atherogenesis in diabetes. J Clin Invest. 2017;127:2133-2147 pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig 2h
Lang S, Harre U, Purohit P, Dietel K, Kienhöfer D, Hahn J, et al. Neurodegeneration Enhances the Development of Arthritis. J Immunol. 2017;198:2394-2402 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1e
Wei Y, Lu C, Chen J, Cui G, Wang L, Yu T, et al. High salt diet stimulates gut Th17 response and exacerbates TNBS-induced colitis in mice. Oncotarget. 2017;8:70-82 pubmed publisher
  • flow cytometry; mouse; fig 4
Shade K, Platzer B, Washburn N, Mani V, Bartsch Y, Conroy M, et al. A single glycan on IgE is indispensable for initiation of anaphylaxis. J Exp Med. 2015;212:457-67 pubmed publisher
  • flow cytometry; mouse
Makarem M, Kannan N, Nguyen L, Knapp D, Balani S, Prater M, et al. Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells. PLoS Biol. 2013;11:e1001630 pubmed publisher
Luo L, Wei D, Pan Y, Wang Q, Feng J, Yu B, et al. MFN2 suppresses clear cell renal cell carcinoma progression by modulating mitochondria-dependent dephosphorylation of EGFR. Cancer Commun (Lond). 2023;43:808-833 pubmed publisher
x160 vec J, x160 x165 astn xe1 M, Jane x10d kov xe1 L, Hr x10d kul xe1 k D, Vojt x11b chov xe1 M, Onhajzer J, et al. TROP2 Represents a Negative Prognostic Factor in Colorectal Adenocarcinoma and Its Expression Is Associated with Features of Epithelial-Mesenchymal Transition and Invasiveness. Cancers (Basel). 2022;14: pubmed publisher
Vanuytsel K, Villacorta Martin C, Lindstrom Vautrin J, Wang Z, Garcia Beltran W, Vrbanac V, et al. Multi-modal profiling of human fetal liver hematopoietic stem cells reveals the molecular signature of engraftment. Nat Commun. 2022;13:1103 pubmed publisher
Tang S, Shi L, Luker B, Mickler C, Suresh B, Lesinski G, et al. Modulation of the tumor microenvironment by armed vesicular stomatitis virus in a syngeneic pancreatic cancer model. Virol J. 2022;19:32 pubmed publisher
Leary N, Walser S, He Y, Cousin N, Pereira P, Gallo A, et al. Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes. J Extracell Vesicles. 2022;11:e12197 pubmed publisher
Barkaway A, Rolas L, Joulia R, Bodkin J, Lenn T, Owen Woods C, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity. 2021;54:1494-1510.e7 pubmed publisher
Sasai M, Ma J, Okamoto M, Nishino K, Nagaoka H, Takashima E, et al. Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells. J Exp Med. 2021;218: pubmed publisher
Tacconi C, Commerford C, Dieterich L, Schwager S, He Y, Ikenberg K, et al. CD169+ lymph node macrophages have protective functions in mouse breast cancer metastasis. Cell Rep. 2021;35:108993 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Stahnke S, Döring H, Kusch C, de Gorter D, Dütting S, Guledani A, et al. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. Curr Biol. 2021;31:2051-2064.e8 pubmed publisher
Chen Y, Kim J, Yang S, Wang H, Wu C, Sugimoto H, et al. Type I collagen deletion in αSMA+ myofibroblasts augments immune suppression and accelerates progression of pancreatic cancer. Cancer Cell. 2021;39:548-565.e6 pubmed publisher
Hemanthakumar K, Fang S, Anisimov A, Mäyränpää M, Mervaala E, Kivela R. Cardiovascular disease risk factors induce mesenchymal features and senescence in mouse cardiac endothelial cells. elife. 2021;10: pubmed publisher
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Huang S, Ziegler C, Austin J, Mannoun N, Vukovic M, Ordovas Montanes J, et al. Lymph nodes are innervated by a unique population of sensory neurons with immunomodulatory potential. Cell. 2021;184:441-459.e25 pubmed publisher
Song J, Frieler R, Whitesall S, Chung Y, Vigil T, Muir L, et al. Myeloid interleukin-4 receptor α is essential in postmyocardial infarction healing by regulating inflammation and fibrotic remodeling. Am J Physiol Heart Circ Physiol. 2021;320:H323-H337 pubmed publisher
Hayashi K, Nikolos F, Lee Y, Jain A, Tsouko E, Gao H, et al. Tipping the immunostimulatory and inhibitory DAMP balance to harness immunogenic cell death. Nat Commun. 2020;11:6299 pubmed publisher
León Letelier R, Castro Medina D, Badillo Godinez O, Tepale Segura A, Huanosta Murillo E, Aguilar Flores C, et al. Induction of Progenitor Exhausted Tissue-Resident Memory CD8+ T Cells Upon Salmonella Typhi Porins Adjuvant Immunization Correlates With Melanoma Control and Anti-PD-1 Immunotherapy Cooperation. Front Immunol. 2020;11:583382 pubmed publisher
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Barsoumian H, Ramapriyan R, Younes A, Caetano M, Menon H, Comeaux N, et al. Low-dose radiation treatment enhances systemic antitumor immune responses by overcoming the inhibitory stroma. J Immunother Cancer. 2020;8: pubmed publisher
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed publisher
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Pfirschke C, Engblom C, Gungabeesoon J, Lin Y, Rickelt S, Zilionis R, et al. Tumor-Promoting Ly-6G+ SiglecFhigh Cells Are Mature and Long-Lived Neutrophils. Cell Rep. 2020;32:108164 pubmed publisher
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Fernandes R, Li C, Wang G, Yang X, Savvides C, Glassman C, et al. Discovery of surrogate agonists for visceral fat Treg cells that modulate metabolic indices in vivo. elife. 2020;9: pubmed publisher
Lebel M, Coutelier M, Galipeau M, Kleinman C, Moon J, Melichar H. Differential expression of tissue-restricted antigens among mTEC is associated with distinct autoreactive T cell fates. Nat Commun. 2020;11:3734 pubmed publisher
Zabala M, Lobo N, Antony J, Heitink L, Gulati G, Lam J, et al. LEFTY1 Is a Dual-SMAD Inhibitor that Promotes Mammary Progenitor Growth and Tumorigenesis. Cell Stem Cell. 2020;27:284-299.e8 pubmed publisher
Heim C, Bosch M, Yamada K, Aldrich A, Chaudhari S, Klinkebiel D, et al. Lactate production by Staphylococcus aureus biofilm inhibits HDAC11 to reprogramme the host immune response during persistent infection. Nat Microbiol. 2020;5:1271-1284 pubmed publisher
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WIETECHA M, Pensalfini M, Cangkrama M, Muller B, Jin J, Brinckmann J, et al. Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds. Nat Commun. 2020;11:2604 pubmed publisher
Ramanan D, Sefik E, Galván Peña S, Wu M, Yang L, Yang Z, et al. An Immunologic Mode of Multigenerational Transmission Governs a Gut Treg Setpoint. Cell. 2020;181:1276-1290.e13 pubmed publisher
Lv Z, Zhang P, Li D, Qin M, Nie L, Wang X, et al. CD19-targeting fusion protein combined with PD1 antibody enhances anti-tumor immunity in mouse models. Oncoimmunology. 2020;9:1747688 pubmed publisher
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Garcia Fabiani M, Comba A, Kadiyala P, Haase S, Núñez F, Altshuler D, et al. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system. Methods Enzymol. 2020;632:369-388 pubmed publisher
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Dieterich L, Kapaklikaya K, Cetintas T, Proulx S, Commerford C, Ikenberg K, et al. Transcriptional profiling of breast cancer-associated lymphatic vessels reveals VCAM-1 as regulator of lymphatic invasion and permeability. Int J Cancer. 2019;145:2804-2815 pubmed publisher
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Su W, Han H, Wang Y, Zhang B, Zhou B, Cheng Y, et al. The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression. Cancer Cell. 2019;36:139-155.e10 pubmed publisher
Omenetti S, Bussi C, Metidji A, Iseppon A, Lee S, Tolaini M, et al. The Intestine Harbors Functionally Distinct Homeostatic Tissue-Resident and Inflammatory Th17 Cells. Immunity. 2019;: pubmed publisher
Qiao J, Liu Z, Dong C, Luan Y, Zhang A, Moore C, et al. Targeting Tumors with IL-10 Prevents Dendritic Cell-Mediated CD8+ T Cell Apoptosis. Cancer Cell. 2019;35:901-915.e4 pubmed publisher
Dubey L, Ludewig B, Luther S, Harris N. IL-4Rα-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells. Cell Rep. 2019;27:2442-2458.e5 pubmed publisher
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Lee D, Jaggi U, Ghiasi H. CCR2+ migratory macrophages with M1 status are the early-responders in the cornea of HSV-1 infected mice. PLoS ONE. 2019;14:e0215727 pubmed publisher
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Zhang F, Li R, Yang Y, Shi C, Shen Y, Lu C, et al. Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses. Immunity. 2019;50:738-750.e7 pubmed publisher
Aoto M, Iwashita A, Mita K, Ohkubo N, Tsujimoto Y, Mitsuda N. Transferrin receptor 1 is required for enucleation of mouse erythroblasts during terminal differentiation. FEBS Open Bio. 2019;9:291-303 pubmed publisher
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Hartwig T, Zwicky P, Schreiner B, Yawalkar N, Cheng P, Navarini A, et al. Regulatory T Cells Restrain Pathogenic T Helper Cells during Skin Inflammation. Cell Rep. 2018;25:3564-3572.e4 pubmed publisher
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Girbl T, Lenn T, Pérez L, Rolas L, Barkaway A, Thiriot A, et al. Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis. Immunity. 2018;49:1062-1076.e6 pubmed publisher
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Chia J, McRae J, Enjapoori A, Lefèvre C, Kukuljan S, Dwyer K. Dietary Cows' Milk Protein A1 Beta-Casein Increases the Incidence of T1D in NOD Mice. Nutrients. 2018;10: pubmed publisher
Di Liberto G, Pantelyushin S, Kreutzfeldt M, Page N, Musardo S, Coras R, et al. Neurons under T Cell Attack Coordinate Phagocyte-Mediated Synaptic Stripping. Cell. 2018;175:458-471.e19 pubmed publisher
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Li Y, Hermanson D, Moriarity B, Kaufman D. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell. 2018;23:181-192.e5 pubmed publisher
Thurlow L, Joshi G, Richardson A. Peroxisome Proliferator-Activated Receptor γ Is Essential for the Resolution of Staphylococcus aureus Skin Infections. Cell Host Microbe. 2018;24:261-270.e4 pubmed publisher
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Kato K, Diéguez Hurtado R, Park D, Hong S, Kato Azuma S, Adams S, et al. Pulmonary pericytes regulate lung morphogenesis. Nat Commun. 2018;9:2448 pubmed publisher
Li C, DiSpirito J, Zemmour D, Spallanzani R, Kuswanto W, Benoist C, et al. TCR Transgenic Mice Reveal Stepwise, Multi-site Acquisition of the Distinctive Fat-Treg Phenotype. Cell. 2018;174:285-299.e12 pubmed publisher
Niknam S, Barsoumian H, Schoenhals J, Jackson H, Yanamandra N, Caetano M, et al. Radiation Followed by OX40 Stimulation Drives Local and Abscopal Antitumor Effects in an Anti-PD1-Resistant Lung Tumor Model. Clin Cancer Res. 2018;24:5735-5743 pubmed publisher
Li A, Barsoumian H, Schoenhals J, Cushman T, Caetano M, Wang X, et al. Indoleamine 2,3-dioxygenase 1 inhibition targets anti-PD1-resistant lung tumors by blocking myeloid-derived suppressor cells. Cancer Lett. 2018;431:54-63 pubmed publisher
Tucey T, Verma J, Harrison P, Snelgrove S, Lo T, Scherer A, et al. Glucose Homeostasis Is Important for Immune Cell Viability during Candida Challenge and Host Survival of Systemic Fungal Infection. Cell Metab. 2018;27:988-1006.e7 pubmed publisher
Iwata T, Ishii C, Ishida S, Ogitani Y, Wada T, Agatsuma T. A HER2-Targeting Antibody-Drug Conjugate, Trastuzumab Deruxtecan (DS-8201a), Enhances Antitumor Immunity in a Mouse Model. Mol Cancer Ther. 2018;17:1494-1503 pubmed publisher
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Mrdjen D, Pavlovic A, Hartmann F, Schreiner B, Utz S, Leung B, et al. High-Dimensional Single-Cell Mapping of Central Nervous System Immune Cells Reveals Distinct Myeloid Subsets in Health, Aging, and Disease. Immunity. 2018;48:380-395.e6 pubmed publisher
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Engblom C, Pfirschke C, Zilionis R, da Silva Martins J, Bos S, Courties G, et al. Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils. Science. 2017;358: pubmed publisher
Eilken H, Diéguez Hurtado R, Schmidt I, Nakayama M, Jeong H, Arf H, et al. Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1. Nat Commun. 2017;8:1574 pubmed publisher
Yu X, Buttgereit A, Lelios I, Utz S, Cansever D, Becher B, et al. The Cytokine TGF-β Promotes the Development and Homeostasis of Alveolar Macrophages. Immunity. 2017;47:903-912.e4 pubmed publisher
Peuhu E, Salomaa S, De Franceschi N, Potter C, Sundberg J, Pouwels J. Integrin beta 1 inhibition alleviates the chronic hyperproliferative dermatitis phenotype of SHARPIN-deficient mice. PLoS ONE. 2017;12:e0186628 pubmed publisher
Lee S, Wilen C, Orvedahl A, McCune B, Kim K, Orchard R, et al. Norovirus Cell Tropism Is Determined by Combinatorial Action of a Viral Non-structural Protein and Host Cytokine. Cell Host Microbe. 2017;22:449-459.e4 pubmed publisher
Huang Z, Zhong L, Lee J, Zhang J, Wu D, Geng L, et al. The FGF21-CCL11 Axis Mediates Beiging of White Adipose Tissues by Coupling Sympathetic Nervous System to Type 2 Immunity. Cell Metab. 2017;26:493-508.e4 pubmed publisher
Williams J, Elvington A, Ivanov S, Kessler S, Luehmann H, Baba O, et al. Thermoneutrality but Not UCP1 Deficiency Suppresses Monocyte Mobilization Into Blood. Circ Res. 2017;121:662-676 pubmed publisher
Domínguez Andrés J, Feo Lucas L, Minguito de la Escalera M, Gonzalez L, López Bravo M, Ardavin C. Inflammatory Ly6Chigh Monocytes Protect against Candidiasis through IL-15-Driven NK Cell/Neutrophil Activation. Immunity. 2017;46:1059-1072.e4 pubmed publisher
Thiriot A, Perdomo C, Cheng G, Novitzky Basso I, McArdle S, Kishimoto J, et al. Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues. BMC Biol. 2017;15:45 pubmed publisher
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product information
Antigen :
CD45
Apps. Abbrev. :
FC
Cat # :
103126
Clone :
30-F11
Item :
Pacific Blue™ anti-mouse CD45
Isotype :
Rat IgG2b, κ
Other Names :
T200, Ly-5, LCA
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation^3, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting^7, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA