product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
APC anti-mouse CD45
catalog :
103111
quantity :
25 μg
price :
33 USD
clonality :
monoclonal
host :
rat
conjugate :
APC
clone name :
30-F11
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 154
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s5a
Liu Y, Du J, Liu X, Wang L, Han Y, Huang C, et al. MG149 inhibits histone acetyltransferase KAT8-mediated IL-33 acetylation to alleviate allergic airway inflammation and airway hyperresponsiveness. Signal Transduct Target Ther. 2021;6:321 pubmed publisher
  • flow cytometry; mouse; 1:400; loading ...; fig 2f
Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
  • flow cytometry; mouse; 1:200; loading ...; fig 5e
Van De Velde L, Allen E, Crawford J, Wilson T, Guy C, Russier M, et al. Neuroblastoma Formation Requires Unconventional CD4 T Cells and Arginase-1-Dependent Myeloid Cells. Cancer Res. 2021;81:5047-5059 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2a, s2b
Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1g
Yang Y, Leonard M, Luo Z, Yeo S, Bick G, Hao M, et al. Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer. Cell Rep. 2021;34:108822 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • flow cytometry; human; loading ...
Wong S, Lenzini S, Cooper M, Mooney D, Shin J. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Sci Adv. 2020;6:eaaw0158 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...; fig 2a
Hou M, Han J, Li G, Kwon M, Jiang J, Emani S, et al. Multipotency of mouse trophoblast stem cells. Stem Cell Res Ther. 2020;11:55 pubmed publisher
  • flow cytometry; mouse; fig e2a
Strickley J, Messerschmidt J, Awad M, Li T, Hasegawa T, Ha D, et al. Immunity to commensal papillomaviruses protects against skin cancer. Nature. 2019;: pubmed publisher
  • flow cytometry; mouse; loading ...
Lopez M, Seyed Razavi Y, Jamali A, Harris D, Hamrah P. The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea. Invest Ophthalmol Vis Sci. 2018;59:5671-5681 pubmed publisher
  • flow cytometry; mouse; fig 2a
James K, Cosway E, LUCAS B, White A, Parnell S, Carvalho Gaspar M, et al. Endothelial cells act as gatekeepers for LTβR-dependent thymocyte emigration. J Exp Med. 2018;215:2984-2993 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3f
Pan D, Kobayashi A, Jiang P, Ferrari de Andrade L, Tay R, Luoma A, et al. A major chromatin regulator determines resistance of tumor cells to T cell-mediated killing. Science. 2018;359:770-775 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig 2g
Lang S, Harre U, Purohit P, Dietel K, Kienhöfer D, Hahn J, et al. Neurodegeneration Enhances the Development of Arthritis. J Immunol. 2017;198:2394-2402 pubmed publisher
  • flow cytometry; mouse
Mangum L, CROW J, Stokes J, HOWELL G, Ross M, Pruett S, et al. Exposure to p,p'-DDE Alters Macrophage Reactivity and Increases Macrophage Numbers in Adipose Stromal Vascular Fraction. Toxicol Sci. 2016;150:169-77 pubmed publisher
  • flow cytometry; mouse
Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, et al. TGFβ signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE. 2015;10:e0117908 pubmed publisher
  • flow cytometry; mouse
Maruyama A, Shime H, Takeda Y, Azuma M, Matsumoto M, Seya T. Pam2 lipopeptides systemically increase myeloid-derived suppressor cells through TLR2 signaling. Biochem Biophys Res Commun. 2015;457:445-50 pubmed publisher
  • flow cytometry; mouse; fig s2
Cagnet S, Faraldo M, Kreft M, Sonnenberg A, Raymond K, Glukhova M. Signaling events mediated by ?3?1 integrin are essential for mammary tumorigenesis. Oncogene. 2014;33:4286-95 pubmed publisher
  • flow cytometry; mouse
Makarem M, Kannan N, Nguyen L, Knapp D, Balani S, Prater M, et al. Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells. PLoS Biol. 2013;11:e1001630 pubmed publisher
Limbad C, Doi R, McGirr J, Ciotlos S, Perez K, Clayton Z, et al. Senolysis induced by 25-hydroxycholesterol targets CRYAB in multiple cell types. iScience. 2022;25:103848 pubmed publisher
Yang Y, Wu J, Li Q, Wang J, Mu L, Hui L, et al. A non-bactericidal cathelicidin provides prophylactic efficacy against bacterial infection by driving phagocyte influx. elife. 2022;11: pubmed publisher
Song X, Li N, Liu Y, Wang Z, Wang T, Tan S, et al. CD169-positive macrophages enhance abscopal effect of radiofrequency ablation therapy in liver cancer. Transl Oncol. 2022;15:101306 pubmed publisher
Ankawa R, Goldberger N, Yosefzon Y, Koren E, Yusupova M, Rösner D, et al. Apoptotic cells represent a dynamic stem cell niche governing proliferation and tissue regeneration. Dev Cell. 2021;56:1900-1916.e5 pubmed publisher
Yu K, Hammerschmidt S, Permanyer M, Galla M, Rothe M, Zheng X, et al. Targeted delivery of regulatory macrophages to lymph nodes interferes with T cell priming by preventing the formation of stable immune synapses. Cell Rep. 2021;35:109273 pubmed publisher
Wosczyna M, Perez Carbajal E, Wagner M, Paredes S, Konishi C, Liu L, et al. Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells. Cell Stem Cell. 2021;28:1323-1334.e8 pubmed publisher
Schiller M, Azulay Debby H, Boshnak N, Elyahu Y, Korin B, Ben Shaanan T, et al. Optogenetic activation of local colonic sympathetic innervations attenuates colitis by limiting immune cell extravasation. Immunity. 2021;54:1022-1036.e8 pubmed publisher
Bravo J, Kim M, Benayoun B. Protocol for isolation of adult mouse ear pinnae-derived primary fibroblasts. STAR Protoc. 2021;2:100406 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Friščić J, Böttcher M, Reinwald C, Bruns H, Wirth B, Popp S, et al. The complement system drives local inflammatory tissue priming by metabolic reprogramming of synovial fibroblasts. Immunity. 2021;54:1002-1021.e10 pubmed publisher
Dawson C, Mueller S, Lindeman G, Rios A, Visvader J. Intravital microscopy of dynamic single-cell behavior in mouse mammary tissue. Nat Protoc. 2021;16:1907-1935 pubmed publisher
Deng P, Yuan Q, Cheng Y, Li J, Liu Z, Liu Y, et al. Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging. Cell Stem Cell. 2021;: pubmed publisher
Ma S, McGuire M, Mangala L, Lee S, Stur E, Hu W, et al. Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype. Cell Rep. 2021;34:108726 pubmed publisher
Almada A, Horwitz N, Price F, Gonzalez A, Ko M, Bölükbaşı Ö, et al. FOS licenses early events in stem cell activation driving skeletal muscle regeneration. Cell Rep. 2021;34:108656 pubmed publisher
Wei Z, Zhang X, Yong T, Bie N, Zhan G, Li X, et al. Boosting anti-PD-1 therapy with metformin-loaded macrophage-derived microparticles. Nat Commun. 2021;12:440 pubmed publisher
Shannon J, Vrba S, Reynoso G, Wynne Jones E, Kamenyeva O, Malo C, et al. Group 1 innate lymphoid-cell-derived interferon-γ maintains anti-viral vigilance in the mucosal epithelium. Immunity. 2021;54:276-290.e5 pubmed publisher
Bagati A, Kumar S, Jiang P, Pyrdol J, Zou A, Godicelj A, et al. Integrin αvβ6-TGFβ-SOX4 Pathway Drives Immune Evasion in Triple-Negative Breast Cancer. Cancer Cell. 2021;39:54-67.e9 pubmed publisher
Kim J, Kolesnikov M, Peled Hajaj S, Scheyltjens I, Xia Y, Trzebanski S, et al. A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages. Immunity. 2021;54:176-190.e7 pubmed publisher
Lv H, Lv G, Chen C, Zong Q, Jiang G, Ye D, et al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Cell Metab. 2021;33:110-127.e5 pubmed publisher
Engler A, Ysasi A, Pihl R, Villacorta Martin C, Heston H, Richardson H, et al. Airway-Associated Macrophages in Homeostasis and Repair. Cell Rep. 2020;33:108553 pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Nosho S, Tosa I, Ono M, Hara E, Ishibashi K, Mikai A, et al. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments. Int J Mol Sci. 2020;21: pubmed publisher
Shemer A, Scheyltjens I, Frumer G, Kim J, Grozovski J, Ayanaw S, et al. Interleukin-10 Prevents Pathological Microglia Hyperactivation following Peripheral Endotoxin Challenge. Immunity. 2020;53:1033-1049.e7 pubmed publisher
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Yeo S, Zhu X, Okamoto T, Hao M, Wang C, Lu P, et al. Single-cell RNA-sequencing reveals distinct patterns of cell state heterogeneity in mouse models of breast cancer. elife. 2020;9: pubmed publisher
JAISWAL A, Makhija S, Stahr N, Sandey M, Suryawanshi A, Mishra A. Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation. Immunobiology. 2020;225:151956 pubmed publisher
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Qu Y, Wen J, Thomas G, Yang W, Prior W, He W, et al. Baseline Frequency of Inflammatory Cxcl9-Expressing Tumor-Associated Macrophages Predicts Response to Avelumab Treatment. Cell Rep. 2020;32:107873 pubmed publisher
Díaz N, Lang R, Van Gelder R, Buhr E. Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. Invest Ophthalmol Vis Sci. 2020;61:37 pubmed publisher
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Zhang T, Wu K, Ma N, Wei L, Garstka M, Zhou W, et al. The C5a/C5aR2 axis promotes renal inflammation and tissue damage. JCI Insight. 2020;5: pubmed publisher
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Cai B, Dongiovanni P, Corey K, Wang X, Shmarakov I, Zheng Z, et al. Macrophage MerTK Promotes Liver Fibrosis in Nonalcoholic Steatohepatitis. Cell Metab. 2020;31:406-421.e7 pubmed publisher
Liu S, Rezende R, Moreira T, Tankou S, Cox L, Wu M, et al. Oral Administration of miR-30d from Feces of MS Patients Suppresses MS-like Symptoms in Mice by Expanding Akkermansia muciniphila. Cell Host Microbe. 2019;26:779-794.e8 pubmed publisher
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Wosczyna M, Konishi C, Perez Carbajal E, Wang T, Walsh R, Gan Q, et al. Mesenchymal Stromal Cells Are Required for Regeneration and Homeostatic Maintenance of Skeletal Muscle. Cell Rep. 2019;27:2029-2035.e5 pubmed publisher
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Duarte N, Coelho I, Holovanchuk D, Inês Almeida J, Penha Gonçalves C, Paula Macedo M. Dipeptidyl Peptidase-4 Is a Pro-Recovery Mediator During Acute Hepatotoxic Damage and Mirrors Severe Shifts in Kupffer Cells. Hepatol Commun. 2018;2:1080-1094 pubmed publisher
Tu W, You L, Tsou A, Chen C. Pten Haplodeficiency Accelerates Liver Tumor Growth in miR-122a-Null Mice via Expansion of Periportal Hepatocyte-Like Cells. Am J Pathol. 2018;188:2688-2702 pubmed publisher
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Wunderlich C, Ackermann P, Ostermann A, Adams Quack P, Vogt M, Tran M, et al. Obesity exacerbates colitis-associated cancer via IL-6-regulated macrophage polarisation and CCL-20/CCR-6-mediated lymphocyte recruitment. Nat Commun. 2018;9:1646 pubmed publisher
Kabir A, Lee T, Pan H, Berry J, Krchma K, Wu J, et al. Requisite endothelial reactivation and effective siRNA nanoparticle targeting of Etv2/Er71 in tumor angiogenesis. JCI Insight. 2018;3: pubmed publisher
Echevarría Vargas I, Reyes Uribe P, Guterres A, Yin X, Kossenkov A, Liu Q, et al. Co-targeting BET and MEK as salvage therapy for MAPK and checkpoint inhibitor-resistant melanoma. EMBO Mol Med. 2018;10: pubmed publisher
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Yuan Z, Kang G, Daharsh L, Fan W, Li Q. SIVcpz closely related to the ancestral HIV-1 is less or non-pathogenic to humans in a hu-BLT mouse model. Emerg Microbes Infect. 2018;7:59 pubmed publisher
Zheng B, Ren T, Huang Y, Sun K, Wang S, Bao X, et al. PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse. J Hematol Oncol. 2018;11:16 pubmed publisher
Su M, Hu R, Song Y, Liu Y, Lai L. Targeted deletion of c-Met in thymic epithelial cells leads to an autoimmune phenotype. Immunol Cell Biol. 2018;96:229-235 pubmed publisher
Bresson L, Faraldo M, Di Cicco A, Quintanilla M, Glukhova M, Deugnier M. Podoplanin regulates mammary stem cell function and tumorigenesis by potentiating Wnt/β-catenin signaling. Development. 2018;145: pubmed publisher
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product information
Antigen :
CD45
Apps. Abbrev. :
FC
Cat # :
103111
Clone :
30-F11
Item :
APC anti-mouse CD45
Isotype :
Rat IgG2b, κ
Other Names :
T200, Ly-5, LCA
Size :
25 μg
Price (USD) :
33 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
APC
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation^3, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting^7, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA