product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse CD45
catalog :
103105
quantity :
50 μg
price :
44 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
30-F11
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 101
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; fig 4c
Cha J, Chan L, Wang Y, Chu Y, Wang C, Lee H, et al. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer. J Biol Chem. 2022;298:101817 pubmed publisher
  • flow cytometry; mouse; 1:500; loading ...; fig s2d
Clayer E, Frank D, Anderton H, Zhang S, Kueh A, Heim V, et al. ZC3H12C expression in dendritic cells is necessary to prevent lymphadenopathy of skin-draining lymph nodes. Immunol Cell Biol. 2022;100:160-173 pubmed publisher
  • flow cytometry; mouse; 1:50; loading ...
Manrique Acevedo C, Padilla J, Naz H, Woodford M, Ghiarone T, Aroor A, et al. Mineralocorticoid Receptor in Myeloid Cells Mediates Angiotensin II-Induced Vascular Dysfunction in Female Mice. Front Physiol. 2021;12:588358 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4d
Chen J, Sivan U, Tan S, Lippo L, De Angelis J, Labella R, et al. High-resolution 3D imaging uncovers organ-specific vascular control of tissue aging. Sci Adv. 2021;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig s16a
Schafflick D, Xu C, Hartlehnert M, Cole M, Schulte Mecklenbeck A, Lautwein T, et al. Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis. Nat Commun. 2020;11:247 pubmed publisher
  • flow cytometry; mouse; 1:800; fig s15a
Kimura S, Nakamura Y, Kobayashi N, Shiroguchi K, Kawakami E, Mutoh M, et al. Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity. Nat Commun. 2020;11:234 pubmed publisher
  • flow cytometry; mouse; 1:100; fig e7b
Dulken B, Buckley M, Navarro Negredo P, Saligrama N, Cayrol R, Leeman D, et al. Single-cell analysis reveals T cell infiltration in old neurogenic niches. Nature. 2019;571:205-210 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s2b
Kobayashi Y, Inagawa H, Kohchi C, Kazumura K, Tsuchiya H, Miwa T, et al. Oral administration of Pantoea agglomerans-derived lipopolysaccharide prevents development of atherosclerosis in high-fat diet-fed apoE-deficient mice via ameliorating hyperlipidemia, pro-inflammatory mediators and oxidative responses. PLoS ONE. 2018;13:e0195008 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1c
Wen G, An W, Chen J, Maguire E, Chen Q, Yang F, et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc. 2018;7: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Yun T, Lee J, Shim D, Choi J, Cheong C. Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis. Methods Mol Biol. 2017;1559:419-437 pubmed publisher
  • flow cytometry; mouse; 1:200; fig s1b
Nakaya M, Watari K, Tajima M, Nakaya T, Matsuda S, Ohara H, et al. Cardiac myofibroblast engulfment of dead cells facilitates recovery after myocardial infarction. J Clin Invest. 2017;127:383-401 pubmed publisher
  • flow cytometry; mouse; fig 5
Li Y, Nishikawa T, Kaneda Y. Platelet-cytokine Complex Suppresses Tumour Growth by Exploiting Intratumoural Thrombin-dependent Platelet Aggregation. Sci Rep. 2016;6:25077 pubmed publisher
  • flow cytometry; mouse; 1:100; fig 4
Watson N, Schneider K, Massa P. SHP-1-dependent macrophage differentiation exacerbates virus-induced myositis. J Immunol. 2015;194:2796-809 pubmed publisher
  • flow cytometry; mouse
Makarem M, Kannan N, Nguyen L, Knapp D, Balani S, Prater M, et al. Developmental changes in the in vitro activated regenerative activity of primitive mammary epithelial cells. PLoS Biol. 2013;11:e1001630 pubmed publisher
Ma L, Tang Q, Gao X, Lee J, Lei R, Suzuki M, et al. Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis. Sci Adv. 2022;8:eabm3470 pubmed publisher
Meng X, Qian X, Ding X, Wang W, Yin X, Zhuang G, et al. Eosinophils regulate intra-adipose axonal plasticity. Proc Natl Acad Sci U S A. 2022;119: pubmed publisher
Li J, Qin X, Shi J, Wang X, Li T, Xu M, et al. A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis. elife. 2021;10: pubmed publisher
Barker K, Etesami N, Shenoy A, Arafa E, Lyon de Ana C, Smith N, et al. Lung-resident memory B cells protect against bacterial pneumonia. J Clin Invest. 2021;131: pubmed publisher
Sun D, Xie X, Zhang X, Wang Z, Sait S, Iyer S, et al. Stem-like cells drive NF1-associated MPNST functional heterogeneity and tumor progression. Cell Stem Cell. 2021;28:1397-1410.e4 pubmed publisher
Bota Rabassedas N, Banerjee P, Niu Y, Cao W, Luo J, Xi Y, et al. Contextual cues from cancer cells govern cancer-associated fibroblast heterogeneity. Cell Rep. 2021;35:109009 pubmed publisher
Vercauteren Drubbel A, Pirard S, Kin S, Dassy B, Lefort A, Libert F, et al. Reactivation of the Hedgehog pathway in esophageal progenitors turns on an embryonic-like program to initiate columnar metaplasia. Cell Stem Cell. 2021;28:1411-1427.e7 pubmed publisher
Walsh S, Sheridan R, Lucas E, Doan T, Ware B, Schafer J, et al. Molecular tracking devices quantify antigen distribution and archiving in the murine lymph node. elife. 2021;10: pubmed publisher
Liu K, Yang L, Wang G, Liu J, Zhao X, Wang Y, et al. Metabolic stress drives sympathetic neuropathy within the liver. Cell Metab. 2021;33:666-675.e4 pubmed publisher
Srivastava S, Furlan S, Jaeger Ruckstuhl C, Sarvothama M, Berger C, Smythe K, et al. Immunogenic Chemotherapy Enhances Recruitment of CAR-T Cells to Lung Tumors and Improves Antitumor Efficacy when Combined with Checkpoint Blockade. Cancer Cell. 2021;39:193-208.e10 pubmed publisher
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Fan C, Feng J, Tang C, Zhang Z, Feng Y, Duan W, et al. Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage. Stem Cell Res Ther. 2020;11:442 pubmed publisher
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Moreno García Á, Bernal Chico A, Colomer T, Rodríguez Antigüedad A, Matute C, Mato S. Gene Expression Analysis of Astrocyte and Microglia Endocannabinoid Signaling during Autoimmune Demyelination. Biomolecules. 2020;10: pubmed publisher
Liu X, Boyer M, Holmgren A, Shin S. Legionella-Infected Macrophages Engage the Alveolar Epithelium to Metabolically Reprogram Myeloid Cells and Promote Antibacterial Inflammation. Cell Host Microbe. 2020;28:683-698.e6 pubmed publisher
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Kamata T, So T, Ahmed Q, Giblett S, Patel B, Luo J, et al. Fibroblast-Derived STC-1 Modulates Tumor-Associated Macrophages and Lung Adenocarcinoma Development. Cell Rep. 2020;31:107802 pubmed publisher
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Bao Z, Li J, Zhang P, Pan Q, Liu B, Zhu J, et al. Toll-Like Receptor 3 Activator Preconditioning Enhances Modulatory Function of Adipose‑Derived Mesenchymal Stem Cells in a Fully MHC-Mismatched Murine Model of Heterotopic Heart Transplantation. Ann Transplant. 2020;25:e921287 pubmed publisher
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Wei H, Xu Y, Chen Q, Chen H, Zhu X, Li Y. Mesenchymal stem cell-derived exosomal miR-223 regulates neuronal cell apoptosis. Cell Death Dis. 2020;11:290 pubmed publisher
Chauveau A, Pirgova G, Cheng H, De Martin A, Zhou F, Wideman S, et al. Visualization of T Cell Migration in the Spleen Reveals a Network of Perivascular Pathways that Guide Entry into T Zones. Immunity. 2020;52:794-807.e7 pubmed publisher
Wijewarnasuriya D, Bebernitz C, Lopez A, Rafiq S, Brentjens R. Excessive Costimulation Leads to Dysfunction of Adoptively Transferred T Cells. Cancer Immunol Res. 2020;8:732-742 pubmed publisher
Riley J, McClain L, Stratigis J, Coons B, Ahn N, Li H, et al. Regulatory T cells promote alloengraftment in a model of late-gestation in utero hematopoietic cell transplantation. Blood Adv. 2020;4:1102-1114 pubmed publisher
Wu N, Cernysiov V, Davidson D, Song H, Tang J, Luo S, et al. Critical Role of Lipid Scramblase TMEM16F in Phosphatidylserine Exposure and Repair of Plasma Membrane after Pore Formation. Cell Rep. 2020;30:1129-1140.e5 pubmed publisher
Wilson D, Jarman E, Mellin R, Wilson M, Waddell S, Tsokkou P, et al. Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors. Nat Commun. 2020;11:445 pubmed publisher
Hillel Karniel C, Rosen C, Milman Krentsis I, Orgad R, Bachar Lustig E, Shezen E, et al. Multi-lineage Lung Regeneration by Stem Cell Transplantation across Major Genetic Barriers. Cell Rep. 2020;30:807-819.e4 pubmed publisher
Khorooshi R, Marczynska J, Dieu R, Wais V, Hansen C, Kavan S, et al. Innate signaling within the central nervous system recruits protective neutrophils. Acta Neuropathol Commun. 2020;8:2 pubmed publisher
Giorgetti E, Panesar M, Zhang Y, Joller S, Ronco M, Obrecht M, et al. Modulation of Microglia by Voluntary Exercise or CSF1R Inhibition Prevents Age-Related Loss of Functional Motor Units. Cell Rep. 2019;29:1539-1554.e7 pubmed publisher
Abels E, Maas S, Nieland L, Wei Z, Cheah P, Tai E, et al. Glioblastoma-Associated Microglia Reprogramming Is Mediated by Functional Transfer of Extracellular miR-21. Cell Rep. 2019;28:3105-3119.e7 pubmed publisher
Ogawa M, Inomata T, Shiang T, Tsubota K, Murakami A. Method for selective quantification of immune and inflammatory cells in the cornea using flow cytometry. J Biol Methods. 2018;5:e102 pubmed publisher
Shmeleva E, Smith G, Ferguson B. Enhanced Efficacy of Vaccination With Vaccinia Virus in Old vs. Young Mice. Front Immunol. 2019;10:1780 pubmed publisher
Fang Y, Zhu L, An N, Jiang G, Qian J, Zhao R, et al. Blood autophagy defect causes accelerated non-hematopoietic organ aging. Aging (Albany NY). 2019;11:4910-4922 pubmed publisher
Chan L, Li C, Xia W, Hsu J, Lee H, Cha J, et al. IL-6/JAK1 pathway drives PD-L1 Y112 phosphorylation to promote cancer immune evasion. J Clin Invest. 2019;129:3324-3338 pubmed publisher
Ye Z, Zhang S, Liu Y, Wang S, Zhang J, Huang R. A Peptide Analogue of Selectin Ligands Attenuated Atherosclerosis by Inhibiting Monocyte Activation. Mediators Inflamm. 2019;2019:8709583 pubmed publisher
Xu L, Yuan N, Liu H, Fang Y, Ge C, Xu F, et al. Bafilomycin A1 targets patient-derived CD34CD19 leukemia stem cells. Haematologica. 2019;: pubmed publisher
Meixiong J, Anderson M, Limjunyawong N, Sabbagh M, Hu E, Mack M, et al. Activation of Mast-Cell-Expressed Mas-Related G-Protein-Coupled Receptors Drives Non-histaminergic Itch. Immunity. 2019;50:1163-1171.e5 pubmed publisher
Miska J, Lee Chang C, Rashidi A, Muroski M, Chang A, Lopez Rosas A, et al. HIF-1α Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma. Cell Rep. 2019;27:226-237.e4 pubmed publisher
Wang G, Zhang L, Zhao X, Gao S, Qu L, Yu H, et al. The Aryl hydrocarbon receptor mediates tobacco-induced PD-L1 expression and is associated with response to immunotherapy. Nat Commun. 2019;10:1125 pubmed publisher
Green D, Limjunyawong N, Gour N, Pundir P, Dong X. A Mast-Cell-Specific Receptor Mediates Neurogenic Inflammation and Pain. Neuron. 2019;101:412-420.e3 pubmed publisher
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Djokić V, Primus S, Akoolo L, Chakraborti M, Parveen N. Age-Related Differential Stimulation of Immune Response by Babesia microti and Borrelia burgdorferi During Acute Phase of Infection Affects Disease Severity. Front Immunol. 2018;9:2891 pubmed publisher
Karlen S, Miller E, Wang X, Levine E, Zawadzki R, Burns M. Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration. J Neuroinflammation. 2018;15:344 pubmed publisher
Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
Chen Z, Amro E, Becker F, Holzer M, Rasa S, Njeru S, et al. Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation. J Exp Med. 2019;216:152-175 pubmed publisher
Tong Y, Zhou L, Yang L, Guo P, Cao Y, Qin F, et al. Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production. EBioMedicine. 2019;39:132-144 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Genoud V, Marinari E, Nikolaev S, Castle J, Bukur V, Dietrich P, et al. Responsiveness to anti-PD-1 and anti-CTLA-4 immune checkpoint blockade in SB28 and GL261 mouse glioma models. Oncoimmunology. 2018;7:e1501137 pubmed publisher
Wang T, Fan C, Yao A, Xu X, Zheng G, You Y, et al. The Adaptor Protein CARD9 Protects against Colon Cancer by Restricting Mycobiota-Mediated Expansion of Myeloid-Derived Suppressor Cells. Immunity. 2018;49:504-514.e4 pubmed publisher
Duarte N, Coelho I, Holovanchuk D, Inês Almeida J, Penha Gonçalves C, Paula Macedo M. Dipeptidyl Peptidase-4 Is a Pro-Recovery Mediator During Acute Hepatotoxic Damage and Mirrors Severe Shifts in Kupffer Cells. Hepatol Commun. 2018;2:1080-1094 pubmed publisher
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Lehmann M, Weigel T, Cooper H, Elkahloun A, Kigar S, Herkenham M. Decoding microglia responses to psychosocial stress reveals blood-brain barrier breakdown that may drive stress susceptibility. Sci Rep. 2018;8:11240 pubmed publisher
Wang Q, Song A, Chen W, Schwalie P, Zhang F, Vishvanath L, et al. Reversible De-differentiation of Mature White Adipocytes into Preadipocyte-like Precursors during Lactation. Cell Metab. 2018;28:282-288.e3 pubmed publisher
Hsu J, Xia W, Hsu Y, Chan L, Yu W, Cha J, et al. STT3-dependent PD-L1 accumulation on cancer stem cells promotes immune evasion. Nat Commun. 2018;9:1908 pubmed publisher
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Stone S, Wu S, Jamison S, Durose W, Pallais J, Lin W. Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases. Glia. 2018;66:1331-1345 pubmed publisher
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product information
Antigen :
CD45
Apps. Abbrev. :
FC
Cat # :
103105
Clone :
30-F11
Item :
PE anti-mouse CD45
Isotype :
Rat IgG2b, κ
Other Names :
T200, Ly-5, LCA
Size :
50 μg
Price (USD) :
44 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
Mouse thymus or spleen
Application Notes :
Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.Additional reported applications (for relevant formats) include: immunoprecipitation^3, complement-dependent cytotoxicity^1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)^4,6, Western blotting^7, and spatial biology (IBEX)^10,11. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA