product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Brilliant Violet 421™ anti-mouse/human CD44
catalog :
103040
quantity :
50 μg
price :
242 USD
clonality :
monoclonal
host :
rat
conjugate :
BV421
clone name :
IM7
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section
more info or order :
citations: 39
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...
Hering L, Katkeviciute E, Schwarzfischer M, Niechcial A, Riggs J, Wawrzyniak M, et al. Macrophages Compensate for Loss of Protein Tyrosine Phosphatase N2 in Dendritic Cells to Protect from Elevated Colitis. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Vuckovic S, Minnie S, Smith D, Gartlan K, Watkins T, Markey K, et al. Bone marrow transplantation generates T cell-dependent control of myeloma in mice. J Clin Invest. 2019;129:106-121 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 2b
Morita K, Okamura T, Inoue M, Komai T, Teruya S, Iwasaki Y, et al. Egr2 and Egr3 in regulatory T cells cooperatively control systemic autoimmunity through Ltbp3-mediated TGF-β3 production. Proc Natl Acad Sci U S A. 2016;113:E8131-E8140 pubmed
Corria Osorio J, Carmona S, Stefanidis E, Andreatta M, Ortiz Miranda Y, Muller T, et al. Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8+ T cells. Nat Immunol. 2023;24:869-883 pubmed publisher
Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
Mugarza E, Van Maldegem F, Boumelha J, Moore C, Rana S, Llorian Sopena M, et al. Therapeutic KRASG12C inhibition drives effective interferon-mediated antitumor immunity in immunogenic lung cancers. Sci Adv. 2022;8:eabm8780 pubmed publisher
Zhu G, Du S, Wang Y, Huang X, Hu G, Lu X, et al. Delayed Antiviral Immune Responses in Severe Acute Respiratory Syndrome Coronavirus Infected Pregnant Mice. Front Microbiol. 2021;12:806902 pubmed publisher
Jiang W, Shi L, Cai L, Wang X, Li J, Li H, et al. A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge. Cell Rep. 2021;37:110112 pubmed publisher
Sandborn W, Mattheakis L, Modi N, Pugatch D, Bressler B, Lee S, et al. PTG-100, an Oral α4β7 Antagonist Peptide: Preclinical Development and Phase 1 and 2a Studies in Ulcerative Colitis. Gastroenterology. 2021;161:1853-1864.e10 pubmed publisher
Marangoni F, Zhakyp A, Corsini M, Geels S, Carrizosa E, Thelen M, et al. Expansion of tumor-associated Treg cells upon disruption of a CTLA-4-dependent feedback loop. Cell. 2021;184:3998-4015.e19 pubmed publisher
Kawakami R, Kitagawa Y, Chen K, Arai M, Ohara D, Nakamura Y, et al. Distinct Foxp3 enhancer elements coordinate development, maintenance, and function of regulatory T cells. Immunity. 2021;54:947-961.e8 pubmed publisher
Kastenschmidt J, Coulis G, Farahat P, Pham P, Rios R, Cristal T, et al. A stromal progenitor and ILC2 niche promotes muscle eosinophilia and fibrosis-associated gene expression. Cell Rep. 2021;35:108997 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Ciesla M, Ngoc P, Cordero E, Martinez Á, Morsing M, Muthukumar S, et al. Oncogenic translation directs spliceosome dynamics revealing an integral role for SF3A3 in breast cancer. Mol Cell. 2021;81:1453-1468.e12 pubmed publisher
Wirasinha R, Davies A, Srivastava M, Sheridan J, Sng X, Delmonte O, et al. Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibility. J Exp Med. 2021;218: pubmed publisher
Fedele C, Li S, Teng K, Foster C, Peng D, Ran H, et al. SHP2 inhibition diminishes KRASG12C cycling and promotes tumor microenvironment remodeling. J Exp Med. 2021;218: pubmed publisher
Wu J, Madi A, Mieg A, Hotz Wagenblatt A, Weisshaar N, Ma S, et al. T Cell Factor 1 Suppresses CD103+ Lung Tissue-Resident Memory T Cell Development. Cell Rep. 2020;31:107484 pubmed publisher
Frohner I, Mudrak I, Schüchner S, Anrather D, Hartl M, Sontag J, et al. PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation. Cell Rep. 2020;30:3171-3182.e6 pubmed publisher
Andersen L, Gülich A, Alteneder M, Preglej T, Orola M, Dhele N, et al. The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3+ Regulatory T Cells. Cell Rep. 2019;29:4447-4459.e6 pubmed publisher
Findlay E, Currie A, Zhang A, Ovciarikova J, Young L, Stevens H, et al. Exposure to the antimicrobial peptide LL-37 produces dendritic cells optimized for immunotherapy. Oncoimmunology. 2019;8:1608106 pubmed publisher
Chow M, Ozga A, Servis R, Frederick D, Lo J, Fisher D, et al. Intratumoral Activity of the CXCR3 Chemokine System Is Required for the Efficacy of Anti-PD-1 Therapy. Immunity. 2019;: pubmed publisher
Jin C, Lagoudas G, Zhao C, Bullman S, Bhutkar A, Hu B, et al. Commensal Microbiota Promote Lung Cancer Development via γδ T Cells. Cell. 2019;176:998-1013.e16 pubmed publisher
Sonzogni O, Haynes J, Seifried L, Kamel Y, Huang K, BeGora M, et al. Reporters to mark and eliminate basal or luminal epithelial cells in culture and in vivo. PLoS Biol. 2018;16:e2004049 pubmed publisher
Li Q, Li D, Zhang X, Wan Q, Zhang W, Zheng M, et al. E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor. Immunity. 2018;48:258-270.e5 pubmed publisher
Slamecka J, McClellan S, Wilk A, Laurini J, Manci E, Hoerstrup S, et al. Induced pluripotent stem cells derived from human amnion in chemically defined conditions. Cell Cycle. 2018;17:330-347 pubmed publisher
Wiese A, Ender F, Quell K, Antoniou K, Vollbrandt T, Konig P, et al. The C5a/C5aR1 axis controls the development of experimental allergic asthma independent of LysM-expressing pulmonary immune cells. PLoS ONE. 2017;12:e0184956 pubmed publisher
Moguche A, Musvosvi M, Penn Nicholson A, Plumlee C, Mearns H, Geldenhuys H, et al. Antigen Availability Shapes T Cell Differentiation and Function during Tuberculosis. Cell Host Microbe. 2017;21:695-706.e5 pubmed publisher
McClellan S, Slamecka J, Howze P, Thompson L, Finan M, Rocconi R, et al. mRNA detection in living cells: A next generation cancer stem cell identification technique. Methods. 2015;82:47-54 pubmed publisher
Yang Y, Gomez J, Herrera M, Perez Marco R, Repenning P, Zhang Z, et al. Salt restriction leads to activation of adult renal mesenchymal stromal cell-like cells via prostaglandin E2 and E-prostanoid receptor 4. Hypertension. 2015;65:1047-54 pubmed publisher
Hirokawa Y, Yip K, Tan C, Burgess A. Colonic myofibroblast cell line stimulates colonoid formation. Am J Physiol Gastrointest Liver Physiol. 2014;306:G547-56 pubmed publisher
Chen Y, Chen K, Huang P, Chen Y, Chiou G, Lo W, et al. Cucurbitacin I suppressed stem-like property and enhanced radiation-induced apoptosis in head and neck squamous carcinoma--derived CD44(+)ALDH1(+) cells. Mol Cancer Ther. 2010;9:2879-92 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Yamazaki J, Mizukami T, Takizawa K, Kuramitsu M, Momose H, Masumi A, et al. Identification of cancer stem cells in a Tax-transgenic (Tax-Tg) mouse model of adult T-cell leukemia/lymphoma. Blood. 2009;114:2709-20 pubmed publisher
Wang X, Lan Y, He W, Zhang L, Yao H, Hou C, et al. Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos. Blood. 2008;111:2436-43 pubmed
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Gui J, Zhu X, Dohkan J, Cheng L, Barnes P, Su D. The aged thymus shows normal recruitment of lymphohematopoietic progenitors but has defects in thymic epithelial cells. Int Immunol. 2007;19:1201-11 pubmed
Chen H, Hou J, Jiang X, Ma S, Meng M, Wang B, et al. Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals. J Immunol. 2005;175:591-8 pubmed
Zhang N, He Y. The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol. 2005;174:6967-73 pubmed
Frank N, Margaryan A, Huang Y, Schatton T, Waaga Gasser A, Gasser M, et al. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005;65:4320-33 pubmed
product information
Antigen :
CD44
Apps. Abbrev. :
FC, ICC, IHC-P
Cat # :
103040
Clone :
IM7
Item :
Brilliant Violet 421™ anti-mouse/human CD44
Isotype :
Rat IgG2b, κ
Other Names :
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Size :
50 μg
Price (USD) :
242 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BV421
Immunogen :
Dexamethasone-induced myeloid leukemia M1 cells
Application Notes :
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186^20. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity^1, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA