product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Pacific Blue™ anti-mouse/human CD44
catalog :
103020
quantity :
100 μg
price :
215 USD
clonality :
monoclonal
host :
rat
conjugate :
Pacific Blue
clone name :
IM7
reactivity :
human, mouse
application :
flow cytometry
more info or order :
citations: 55
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 4b
Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2a
Gounder A, Yokoyama C, Jarjour N, Bricker T, Edelson B, Boon A. Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer. PLoS Pathog. 2018;14:e1007001 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 6b
Bhattacharyya M, Penaloza MacMaster P. T regulatory cells are critical for the maintenance, anamnestic expansion and protection elicited by vaccine-induced CD8 T cells. Immunology. 2017;151:340-348 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
  • flow cytometry; mouse
Mall C, Sckisel G, Proia D, Mirsoian A, Grossenbacher S, Pai C, et al. Repeated PD-1/PD-L1 monoclonal antibody administration induces fatal xenogeneic hypersensitivity reactions in a murine model of breast cancer. Oncoimmunology. 2016;5:e1075114 pubmed
  • flow cytometry; mouse; loading ...; fig 5a
Roffê E, Marino A, Weaver J, Wan W, de Araújo F, Hoffman V, et al. Trypanosoma cruzi Causes Paralyzing Systemic Necrotizing Vasculitis Driven by Pathogen-Specific Type I Immunity in Mice. Infect Immun. 2016;84:1123-1136 pubmed publisher
  • flow cytometry; mouse; fig 1
Nguyen L, Pan J, Dinh T, Hadeiba H, O Hara E, Ebtikar A, et al. Role and species-specific expression of colon T cell homing receptor GPR15 in colitis. Nat Immunol. 2015;16:207-213 pubmed publisher
Wilson A, Randall K, Pettitt J, Ellyard J, Blumenthal A, Enders A, et al. Neutrophil extracellular traps and their histones promote Th17 cell differentiation directly via TLR2. Nat Commun. 2022;13:528 pubmed publisher
Wagle M, Vervoort S, Kelly M, Van Der Byl W, Peters T, Martin B, et al. Antigen-driven EGR2 expression is required for exhausted CD8+ T cell stability and maintenance. Nat Commun. 2021;12:2782 pubmed publisher
Delacher M, Simon M, Sanderink L, Hotz Wagenblatt A, Wuttke M, Schambeck K, et al. Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells. Immunity. 2021;54:702-720.e17 pubmed publisher
Jaeger N, McDonough R, Rosen A, Hernandez Leyva A, Wilson N, Lint M, et al. Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation. Cell Rep. 2020;33:108331 pubmed publisher
Hering L, Katkeviciute E, Schwarzfischer M, Busenhart P, Gottier C, Mrdjen D, et al. Protein Tyrosine Phosphatase Non-Receptor Type 2 Function in Dendritic Cells Is Crucial to Maintain Tissue Tolerance. Front Immunol. 2020;11:1856 pubmed publisher
Pauken K, Godec J, Odorizzi P, Brown K, Yates K, Ngiow S, et al. The PD-1 Pathway Regulates Development and Function of Memory CD8+ T Cells following Respiratory Viral Infection. Cell Rep. 2020;31:107827 pubmed publisher
Vanderleyden I, Fra Bido S, Innocentin S, Stebegg M, Okkenhaug H, Evans Bailey N, et al. Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5. Cell Rep. 2020;30:611-619.e4 pubmed publisher
Kim C, Jadhav R, Gustafson C, Smithey M, Hirsch A, Uhrlaub J, et al. Defects in Antiviral T Cell Responses Inflicted by Aging-Associated miR-181a Deficiency. Cell Rep. 2019;29:2202-2216.e5 pubmed publisher
Yadava K, Medina C, Ishak H, Gurevich I, Kuipers H, Shamskhou E, et al. Natural Tr1-like cells do not confer long-term tolerogenic memory. elife. 2019;8: pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Schmidleithner L, Thabet Y, Schönfeld E, Köhne M, Sommer D, Abdullah Z, et al. Enzymatic Activity of HPGD in Treg Cells Suppresses Tconv Cells to Maintain Adipose Tissue Homeostasis and Prevent Metabolic Dysfunction. Immunity. 2019;50:1232-1248.e14 pubmed publisher
Misumi I, Starmer J, Uchimura T, Beck M, Magnuson T, Whitmire J. Obesity Expands a Distinct Population of T Cells in Adipose Tissue and Increases Vulnerability to Infection. Cell Rep. 2019;27:514-524.e5 pubmed publisher
Ogawa C, Bankoti R, Nguyen T, Hassanzadeh Kiabi N, Nadeau S, Porritt R, et al. Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3+RORγt+ Regulatory T Cells. Cell Rep. 2018;25:19-28.e5 pubmed publisher
Hirata Y, Furuhashi K, Ishii H, Li H, Pinho S, Ding L, et al. CD150high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine. Cell Stem Cell. 2018;22:445-453.e5 pubmed publisher
Kooreman N, Kim Y, de Almeida P, Termglinchan V, Diecke S, Shao N, et al. Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo. Cell Stem Cell. 2018;22:501-513.e7 pubmed publisher
Resch T, Wang Y, Moon S, Joyce J, Li S, PRAUSNITZ M, et al. Inactivated rotavirus vaccine by parenteral administration induces mucosal immunity in mice. Sci Rep. 2018;8:561 pubmed publisher
Bruder J, Chen P, Ekberg G, Smith E, Lazarski C, Myers B, et al. Profiling the Targets of Protective CD8+ T Cell Responses to Infection. Mol Ther Methods Clin Dev. 2017;7:20-31 pubmed publisher
Isoda T, Moore A, He Z, Chandra V, Aida M, Denholtz M, et al. Non-coding Transcription Instructs Chromatin Folding and Compartmentalization to Dictate Enhancer-Promoter Communication and T Cell Fate. Cell. 2017;171:103-119.e18 pubmed publisher
Zhang Y, Kurupati R, Liu L, Zhou X, Zhang G, Hudaihed A, et al. Enhancing CD8+ T Cell Fatty Acid Catabolism within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma Immunotherapy. Cancer Cell. 2017;32:377-391.e9 pubmed publisher
Jena P, Sheng L, Liu H, Kalanetra K, Mirsoian A, Murphy W, et al. Western Diet-Induced Dysbiosis in Farnesoid X Receptor Knockout Mice Causes Persistent Hepatic Inflammation after Antibiotic Treatment. Am J Pathol. 2017;187:1800-1813 pubmed publisher
Sckisel G, Mirsoian A, Minnar C, Crittenden M, Curti B, Chen J, et al. Differential phenotypes of memory CD4 and CD8 T cells in the spleen and peripheral tissues following immunostimulatory therapy. J Immunother Cancer. 2017;5:33 pubmed publisher
Hirako I, Ataide M, Faustino L, Assis P, Sorensen E, Ueta H, et al. Splenic differentiation and emergence of CCR5+CXCL9+CXCL10+ monocyte-derived dendritic cells in the brain during cerebral malaria. Nat Commun. 2016;7:13277 pubmed publisher
Kaji T, Hijikata A, Ishige A, Kitami T, Watanabe T, Ohara O, et al. CD4 memory T cells develop and acquire functional competence by sequential cognate interactions and stepwise gene regulation. Int Immunol. 2016;28:267-82 pubmed publisher
Dieudé M, Bell C, Turgeon J, Beillevaire D, Pomerleau L, Yang B, et al. The 20S proteasome core, active within apoptotic exosome-like vesicles, induces autoantibody production and accelerates rejection. Sci Transl Med. 2015;7:318ra200 pubmed publisher
Burrack K, Tan J, McCarthy M, Her Z, Berger J, Ng L, et al. Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells. PLoS Pathog. 2015;11:e1005191 pubmed publisher
Ames E, Canter R, Grossenbacher S, Mac S, Chen M, Smith R, et al. NK Cells Preferentially Target Tumor Cells with a Cancer Stem Cell Phenotype. J Immunol. 2015;195:4010-9 pubmed publisher
Thomas S, Klobuch S, Podlech J, Plachter B, Hoffmann P, Renzaho A, et al. Evaluating Human T-Cell Therapy of Cytomegalovirus Organ Disease in HLA-Transgenic Mice. PLoS Pathog. 2015;11:e1005049 pubmed publisher
Kim B, Sasaki Y, Egawa T. Restriction of Nonpermissive RUNX3 Protein Expression in T Lymphocytes by the Kozak Sequence. J Immunol. 2015;195:1517-23 pubmed publisher
Pierini A, Colonna L, Alvarez M, Schneidawind D, Nishikii H, Baker J, et al. Donor Requirements for Regulatory T Cell Suppression of Murine Graft-versus-Host Disease. J Immunol. 2015;195:347-55 pubmed publisher
Burgos Ojeda D, Wu R, McLean K, Chen Y, Talpaz M, Yoon E, et al. CD24+ Ovarian Cancer Cells Are Enriched for Cancer-Initiating Cells and Dependent on JAK2 Signaling for Growth and Metastasis. Mol Cancer Ther. 2015;14:1717-27 pubmed publisher
Yang Y, Gomez J, Herrera M, Perez Marco R, Repenning P, Zhang Z, et al. Salt restriction leads to activation of adult renal mesenchymal stromal cell-like cells via prostaglandin E2 and E-prostanoid receptor 4. Hypertension. 2015;65:1047-54 pubmed publisher
Hirokawa Y, Yip K, Tan C, Burgess A. Colonic myofibroblast cell line stimulates colonoid formation. Am J Physiol Gastrointest Liver Physiol. 2014;306:G547-56 pubmed publisher
Michel M, Pang D, Haque S, Potocnik A, Pennington D, Hayday A. Interleukin 7 (IL-7) selectively promotes mouse and human IL-17-producing γδ cells. Proc Natl Acad Sci U S A. 2012;109:17549-54 pubmed publisher
Benoit D, Boutin M. Controlling mesenchymal stem cell gene expression using polymer-mediated delivery of siRNA. Biomacromolecules. 2012;13:3841-9 pubmed publisher
Chang P, Lee S, Hu X, Davey G, Duan G, Cho J, et al. Breakdown in repression of IFN-? mRNA leads to accumulation of self-reactive effector CD8+ T cells. J Immunol. 2012;189:701-10 pubmed publisher
Roffê E, Rothfuchs A, Santiago H, Marino A, Ribeiro Gomes F, Eckhaus M, et al. IL-10 limits parasite burden and protects against fatal myocarditis in a mouse model of Trypanosoma cruzi infection. J Immunol. 2012;188:649-60 pubmed publisher
Salti S, Hammelev E, Grewal J, Reddy S, Zemple S, Grossman W, et al. Granzyme B regulates antiviral CD8+ T cell responses. J Immunol. 2011;187:6301-9 pubmed publisher
Yabas M, Godfrey D, Goodnow C, Hoyne G. Differential requirement for the CD45 splicing regulator hnRNPLL for accumulation of NKT and conventional T cells. PLoS ONE. 2011;6:e26440 pubmed publisher
Chen Y, Chen K, Huang P, Chen Y, Chiou G, Lo W, et al. Cucurbitacin I suppressed stem-like property and enhanced radiation-induced apoptosis in head and neck squamous carcinoma--derived CD44(+)ALDH1(+) cells. Mol Cancer Ther. 2010;9:2879-92 pubmed publisher
Teh C, Daley S, Enders A, Goodnow C. T-cell regulation by casitas B-lineage lymphoma (Cblb) is a critical failsafe against autoimmune disease due to autoimmune regulator (Aire) deficiency. Proc Natl Acad Sci U S A. 2010;107:14709-14 pubmed publisher
Kmieciak M, Gowda M, Graham L, Godder K, Bear H, Marincola F, et al. Human T cells express CD25 and Foxp3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. J Transl Med. 2009;7:89 pubmed publisher
Yamazaki J, Mizukami T, Takizawa K, Kuramitsu M, Momose H, Masumi A, et al. Identification of cancer stem cells in a Tax-transgenic (Tax-Tg) mouse model of adult T-cell leukemia/lymphoma. Blood. 2009;114:2709-20 pubmed publisher
Wang X, Lan Y, He W, Zhang L, Yao H, Hou C, et al. Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos. Blood. 2008;111:2436-43 pubmed
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Gui J, Zhu X, Dohkan J, Cheng L, Barnes P, Su D. The aged thymus shows normal recruitment of lymphohematopoietic progenitors but has defects in thymic epithelial cells. Int Immunol. 2007;19:1201-11 pubmed
Chen H, Hou J, Jiang X, Ma S, Meng M, Wang B, et al. Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals. J Immunol. 2005;175:591-8 pubmed
Zhang N, He Y. The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol. 2005;174:6967-73 pubmed
Frank N, Margaryan A, Huang Y, Schatton T, Waaga Gasser A, Gasser M, et al. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005;65:4320-33 pubmed
product information
Antigen :
CD44
Apps. Abbrev. :
FC
Cat # :
103020
Clone :
IM7
Item :
Pacific Blue™ anti-mouse/human CD44
Isotype :
Rat IgG2b, κ
Other Names :
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Size :
100 μg
Price (USD) :
215 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PB
Immunogen :
Dexamethasone-induced myeloid leukemia M1 cells
Application Notes :
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186^20. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity^1, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA