Alexa Fluor® 647 anti-mouse/human CD44 | BioLegend 103017 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Alexa Fluor® 647 anti-mouse/human CD44

catalog :

103017

quantity :

25 μg

price :

101 USD

clonality :

monoclonal

host :

rat

conjugate :

AF647

clone name :

IM7

reactivity :

human, mouse

application :

immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

BioLegend product webpage

citations: 104

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...	Amaral E, Foreman T, Namasivayam S, Hilligan K, Kauffman K, Barbosa Bomfim C, et al. GPX4 regulates cellular necrosis and host resistance in Mycobacterium tuberculosis infection. J Exp Med. 2022;219: pubmed publisher
flow cytometry; mouse; loading ...; fig 4f	Hou X, Shi Y, Kang X, Rousu Z, Li D, Wang M, et al. Echinococcus granulosus: The establishment of the metacestode in the liver is associated with control of the CD4+ T-cell-mediated immune response in patients with cystic echinococcosis and a mouse model. Front Cell Infect Microbiol. 2022;12:983119 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 4e	Bradford B, McGuire L, Hume D, Pridans C, Mabbott N. Microglia deficiency accelerates prion disease but does not enhance prion accumulation in the brain. Glia. 2022;70:2169-2187 pubmed publisher
flow cytometry; mouse; fig 6a	Laffey K, Stiles R, Ludescher M, Davis T, Khwaja S, Bram R, et al. Early expression of mature αβ TCR in CD4-CD8- T cell progenitors enables MHC to drive development of T-ALL bearing NOTCH mutations. Proc Natl Acad Sci U S A. 2022;119:e2118529119 pubmed publisher
flow cytometry; mouse; 1:100; fig 1g	Wang Q, Bergholz J, Ding L, Lin Z, Kabraji S, Hughes M, et al. STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer. Nat Commun. 2022;13:3022 pubmed publisher
flow cytometry; mouse; loading ...; fig 2c, 3d	Koutn xed k J, Klepsch V, Pommermayr M, Thuille N, Baier G, Siegmund K. A MLR-Based Approach to Analyze Regulators of T Lymphocyte Activation In Vivo. Int J Mol Sci. 2022;23: pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Brown G, Ca xf1 ete P, Wang H, Medhavy A, Bones J, Roco J, et al. TLR7 gain-of-function genetic variation causes human lupus. Nature. 2022;605:349-356 pubmed publisher
flow cytometry; mouse; loading ...; fig 2b	Quách T, Huang W, Sahu R, Diadhiou C, Raparia C, Johnson R, et al. Context-dependent induction of autoimmunity by TNF signaling deficiency. JCI Insight. 2022;7: pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	Susukida T, Kuwahara S, Song B, Kazaoka A, Aoki S, Ito K. Regulation of the immune tolerance system determines the susceptibility to HLA-mediated abacavir-induced skin toxicity. Commun Biol. 2021;4:1137 pubmed publisher
flow cytometry; mouse; 1:300; loading ...; fig 3c, s4b	Moreira T, Mangani D, Cox L, Leibowitz J, Lobo E, Oliveira M, et al. PD-L1+ and XCR1+ dendritic cells are region-specific regulators of gut homeostasis. Nat Commun. 2021;12:4907 pubmed publisher
flow cytometry; mouse; loading ...	Winn N, Wolf E, Cottam M, Bhanot M, Hasty A. Myeloid-specific deletion of ferroportin impairs macrophage bioenergetics but is disconnected from systemic insulin action in adult mice. Am J Physiol Endocrinol Metab. 2021;321:E376-E391 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig s22a	Ho D, Tsui Y, Chan L, Sze K, Zhang X, Cheu J, et al. Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma. Nat Commun. 2021;12:3684 pubmed publisher
flow cytometry; mouse; fig 6a	Okunuki Y, Tabor S, Lee M, Connor K. CD47 Deficiency Ameliorates Ocular Autoimmune Inflammation. Front Immunol. 2021;12:680568 pubmed publisher
flow cytometry; mouse; loading ...; fig 5d	Clemen R, Freund E, Mrochen D, Miebach L, Schmidt A, Rauch B, et al. Gas Plasma Technology Augments Ovalbumin Immunogenicity and OT-II T Cell Activation Conferring Tumor Protection in Mice. Adv Sci (Weinh). 2021;8:2003395 pubmed publisher
flow cytometry; mouse; 1:1000; loading ...; fig 1b	Phong B, D Souza S, Baudier R, Wu E, Immethun V, Bauer D, et al. IgE-activated mast cells enhance TLR4-mediated antigen-specific CD4+ T cell responses. Sci Rep. 2021;11:9686 pubmed publisher
flow cytometry; mouse; loading ...; fig s1a	Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
flow cytometry; mouse; 1:200; fig 1b	Zhang S, Li L, Xie D, Reddy S, Sleasman J, Ma L, et al. Regulation of Intrinsic and Bystander T Follicular Helper Cell Differentiation and Autoimmunity by Tsc1. Front Immunol. 2021;12:620437 pubmed publisher
flow cytometry; mouse; fig 2c	Can xe8 S, Van Snick J, Uyttenhove C, Pilotte L, van den Eynde B. TGFβ1 neutralization displays therapeutic efficacy through both an immunomodulatory and a non-immune tumor-intrinsic mechanism. J Immunother Cancer. 2021;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 5c	Hamminger P, Marchetti L, Preglej T, Platzer R, Zhu C, Kamnev A, et al. Histone deacetylase 1 controls CD4+ T cell trafficking in autoinflammatory diseases. J Autoimmun. 2021;119:102610 pubmed publisher
flow cytometry; mouse; 1:100; loading ...; fig 3c	Break T, Oikonomou V, Dutzan N, Desai J, Swidergall M, Freiwald T, et al. Aberrant type 1 immunity drives susceptibility to mucosal fungal infections. Science. 2021;371: pubmed publisher
flow cytometry; mouse	Webb L, Fra Bido S, Innocentin S, Matheson L, Attaf N, Bignon A, et al. Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ. Aging Cell. 2021;20:e13295 pubmed publisher
flow cytometry; mouse; 1:200; loading ...	Castiello M, Bosticardo M, Sacchetti N, Calzoni E, Fontana E, Yamazaki Y, et al. Efficacy and safety of anti-CD45-saporin as conditioning agent for RAG deficiency. J Allergy Clin Immunol. 2021;147:309-320.e6 pubmed publisher
flow cytometry; mouse; loading ...; fig 2f	Meryk A, Grasse M, Balasco L, Kapferer W, Grubeck Loebenstein B, Pangrazzi L. Antioxidants N-Acetylcysteine and Vitamin C Improve T Cell Commitment to Memory and Long-Term Maintenance of Immunological Memory in Old Mice. Antioxidants (Basel). 2020;9: pubmed publisher
flow cytometry; mouse; 1:100; loading ...	Fernandes R, Li C, Wang G, Yang X, Savvides C, Glassman C, et al. Discovery of surrogate agonists for visceral fat Treg cells that modulate metabolic indices in vivo. elife. 2020;9: pubmed publisher
flow cytometry; human; 1:200; loading ...; fig 3a	Pseftogas A, Xanthopoulos K, Poutahidis T, Ainali C, Dafou D, Panteris E, et al. The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling. Cancers (Basel). 2020;12: pubmed publisher
flow cytometry; mouse; loading ...; fig s5c	Svensson M, Zoccheddu M, Yang S, Nygaard G, Secchi C, Doody K, et al. Synoviocyte-targeted therapy synergizes with TNF inhibition in arthritis reversal. Sci Adv. 2020;6:eaba4353 pubmed publisher
flow cytometry; human; loading ...	Liao T, Lin C, Jiang J, Yang S, Teng H, Yang M. Harnessing stemness and PD-L1 expression by AT-rich interaction domain-containing protein 3B in colorectal cancer. Theranostics. 2020;10:6095-6112 pubmed publisher
flow cytometry; mouse; loading ...; fig 5d	Zheng D, Gao F, Cheng Q, Bao P, Dong X, Fan J, et al. A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy. Nat Commun. 2020;11:1985 pubmed publisher
flow cytometry; mouse; loading ...	Zhu M, Ma Y, Tan K, Zhang L, Wang Z, Li Y, et al. Thalidomide with blockade of co-stimulatory molecules prolongs the survival of alloantigen-primed mice with cardiac allografts. BMC Immunol. 2020;21:19 pubmed publisher
flow cytometry; mouse; 1:200; loading ...; fig 2s1	Stebegg M, Bignon A, Hill D, Silva Cayetano A, Krueger C, Vanderleyden I, et al. Rejuvenating conventional dendritic cells and T follicular helper cell formation after vaccination. elife. 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig s18	von Roemeling C, Wang Y, Qie Y, Yuan H, Zhao H, Liu X, et al. Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity. Nat Commun. 2020;11:1508 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 6a	Donaldson D, Bradford B, Else K, Mabbott N. Accelerated onset of CNS prion disease in mice co-infected with a gastrointestinal helminth pathogen during the preclinical phase. Sci Rep. 2020;10:4554 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Kumar A, Chamoto K, Chowdhury P, Honjo T. Tumors attenuating the mitochondrial activity in T cells escape from PD-1 blockade therapy. elife. 2020;9: pubmed publisher
flow cytometry; mouse; loading ...; fig 1e, 1j	Chen H, Cong X, Wu C, Wu X, Wang J, Mao K, et al. Intratumoral delivery of CCL25 enhances immunotherapy against triple-negative breast cancer by recruiting CCR9+ T cells. Sci Adv. 2020;6:eaax4690 pubmed publisher
flow cytometry; mouse; loading ...; fig s1a	Angenendt A, Steiner R, Knörck A, Schwär G, Konrad M, Krause E, et al. Orai, STIM, and PMCA contribute to reduced calcium signal generation in CD8+ T cells of elderly mice. Aging (Albany NY). 2020;12:3266-3286 pubmed publisher
flow cytometry; mouse; loading ...; fig 2s1b	Canel M, Taggart D, Sims A, Lonergan D, Waizenegger I, Serrels A. T-cell co-stimulation in combination with targeting FAK drives enhanced anti-tumor immunity. elife. 2020;9: pubmed publisher
flow cytometry; mouse; fig s5a	Williford J, Ishihara J, Ishihara A, Mansurov A, Hosseinchi P, Marchell T, et al. Recruitment of CD103+ dendritic cells via tumor-targeted chemokine delivery enhances efficacy of checkpoint inhibitor immunotherapy. Sci Adv. 2019;5:eaay1357 pubmed publisher
flow cytometry; mouse; fig s5b	Wei J, Long L, Zheng W, Dhungana Y, Lim S, Guy C, et al. Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy. Nature. 2019;576:471-476 pubmed publisher
flow cytometry; mouse; loading ...; fig e4d	Hang S, Paik D, Yao L, Kim E, Jamma T, Lu J, et al. Bile acid metabolites control TH17 and Treg cell differentiation. Nature. 2019;576:143-148 pubmed publisher
flow cytometry; mouse; loading ...; fig s1h	Mani V, Bromley S, Aijö T, Mora Buch R, Carrizosa E, Warner R, et al. Migratory DCs activate TGF-β to precondition naïve CD8+ T cells for tissue-resident memory fate. Science. 2019;366: pubmed publisher
flow cytometry; mouse; loading ...; fig 3a	Shikama Y, Kurosawa M, Furukawa M, Ishimaru N, Matsushita K. Involvement of adiponectin in age-related increases in tear production in mice. Aging (Albany NY). 2019;11:8329-8346 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 2a	Noguerol J, Roustan P, N Taye M, Delcombel L, Rolland C, Guiraud L, et al. Sexual dimorphism in PAR2-dependent regulation of primitive colonic cells. Biol Sex Differ. 2019;10:47 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a	Rasoulouniriana D, Santana Magal N, Gutwillig A, Farhat Younis L, Wine Y, Saperia C, et al. A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity. J Clin Invest. 2019;129:4151-4164 pubmed publisher
flow cytometry; mouse; loading ...; fig s4b	Wirsching H, Zhang H, Szulzewsky F, Arora S, Grandi P, Cimino P, et al. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma. JCI Insight. 2019;4: pubmed publisher
flow cytometry; mouse; 1:400; loading ...; fig s6a	Ansaldo E, Slayden L, Ching K, Koch M, Wolf N, Plichta D, et al. Akkermansia muciniphila induces intestinal adaptive immune responses during homeostasis. Science. 2019;364:1179-1184 pubmed publisher
flow cytometry; mouse; loading ...; fig s2e	Di Pilato M, Kim E, Cadilha B, Prüßmann J, Nasrallah M, Seruggia D, et al. Targeting the CBM complex causes Treg cells to prime tumours for immune checkpoint therapy. Nature. 2019;570:112-116 pubmed publisher
flow cytometry; mouse; loading ...; fig 4b	Qiu J, Villa M, Sanin D, Buck M, O Sullivan D, Ching R, et al. Acetate Promotes T Cell Effector Function during Glucose Restriction. Cell Rep. 2019;27:2063-2074.e5 pubmed publisher
flow cytometry; mouse; loading ...; fig s8a	Li Y, Tinoco R, Elmén L, Segota I, Xian Y, Fujita Y, et al. Gut microbiota dependent anti-tumor immunity restricts melanoma growth in Rnf5-/- mice. Nat Commun. 2019;10:1492 pubmed publisher
flow cytometry; mouse; loading ...; fig 2e	Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
flow cytometry; mouse; loading ...; fig 1f	McLaren J, Clement M, Marsden M, Miners K, Llewellyn Lacey S, Grant E, et al. IL-33 Augments Virus-Specific Memory T Cell Inflation and Potentiates the Efficacy of an Attenuated Cytomegalovirus-Based Vaccine. J Immunol. 2019;202:943-955 pubmed publisher
flow cytometry; mouse; loading ...; fig e1b	Karmaus P, Chen X, Lim S, Herrada A, Nguyen T, Xu B, et al. Metabolic heterogeneity underlies reciprocal fates of TH17 cell stemness and plasticity. Nature. 2019;565:101-105 pubmed publisher
flow cytometry; mouse; loading ...; fig s4a	Poncette L, Chen X, Lorenz F, Blankenstein T. Effective NY-ESO-1-specific MHC II-restricted T cell receptors from antigen-negative hosts enhance tumor regression. J Clin Invest. 2019;129:324-335 pubmed publisher
flow cytometry; mouse; loading ...; fig s5d	Sato Y, Bolzenius J, Eteleeb A, Su X, Maher C, Sehn J, et al. CD4+ T cells induce rejection of urothelial tumors after immune checkpoint blockade. JCI Insight. 2018;3: pubmed publisher
flow cytometry; mouse; loading ...; fig 2b	Muscate F, Stetter N, Schramm C, Schulze zur Wiesch J, Bosurgi L, Jacobs T. HVEM and CD160: Regulators of Immunopathology During Malaria Blood-Stage. Front Immunol. 2018;9:2611 pubmed publisher
flow cytometry; mouse; loading ...; fig 2b, 5c	Klement J, Paschall A, Redd P, Ibrahim M, Lu C, Yang D, et al. An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion. J Clin Invest. 2018;128:5549-5560 pubmed publisher
flow cytometry; mouse; loading ...; fig 2a	Vuckovic S, Minnie S, Smith D, Gartlan K, Watkins T, Markey K, et al. Bone marrow transplantation generates T cell-dependent control of myeloma in mice. J Clin Invest. 2019;129:106-121 pubmed publisher
flow cytometry; mouse; 1:400; loading ...; fig 5d	Sang A, Danhorn T, Peterson J, Rankin A, O Connor B, Leach S, et al. Innate and adaptive signals enhance differentiation and expansion of dual-antibody autoreactive B cells in lupus. Nat Commun. 2018;9:3973 pubmed publisher
flow cytometry; mouse; loading ...; fig 3f	Cummings M, Arumanayagam A, Zhao P, Kannanganat S, Stuve O, Karandikar N, et al. Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis. PLoS ONE. 2018;13:e0200752 pubmed publisher
flow cytometry; mouse; loading ...; fig s1c	Arnold I, Artola Borán M, Tallón de Lara P, Kyburz A, Taube C, OTTEMANN K, et al. Eosinophils suppress Th1 responses and restrict bacterially induced gastrointestinal inflammation. J Exp Med. 2018;215:2055-2072 pubmed publisher
flow cytometry; mouse; loading ...; fig s19	Vendetti F, Karukonda P, Clump D, Teo T, Lalonde R, Nugent K, et al. ATR kinase inhibitor AZD6738 potentiates CD8+ T cell-dependent antitumor activity following radiation. J Clin Invest. 2018;128:3926-3940 pubmed publisher
flow cytometry; mouse; fig 2d	Nusse Y, Savage A, Marangoni P, Rosendahl Huber A, Landman T, De Sauvage F, et al. Parasitic helminths induce fetal-like reversion in the intestinal stem cell niche. Nature. 2018;559:109-113 pubmed publisher
flow cytometry; mouse; loading ...; fig e2e	Du X, Wen J, Wang Y, Karmaus P, Khatamian A, Tan H, et al. Hippo/Mst signalling couples metabolic state and immune function of CD8α+ dendritic cells. Nature. 2018;558:141-145 pubmed publisher
flow cytometry; mouse; loading ...; fig s1d	Daenthanasanmak A, Wu Y, Iamsawat S, Nguyen H, Bastian D, Zhang M, et al. PIM-2 protein kinase negatively regulates T cell responses in transplantation and tumor immunity. J Clin Invest. 2018;128:2787-2801 pubmed publisher
flow cytometry; mouse; loading ...; fig s3f	Drobek A, Moudra A, Mueller D, Huranová M, Horková V, Pribikova M, et al. Strong homeostatic TCR signals induce formation of self-tolerant virtual memory CD8 T cells. EMBO J. 2018;37: pubmed publisher
flow cytometry; mouse; loading ...; fig 1h	Gaddis D, Padgett L, Wu R, McSkimming C, Romines V, Taylor A, et al. Apolipoprotein AI prevents regulatory to follicular helper T cell switching during atherosclerosis. Nat Commun. 2018;9:1095 pubmed publisher
flow cytometry; mouse; loading ...; fig s2a	Fahl S, Coffey F, Kain L, Zarin P, Dunbrack R, Teyton L, et al. Role of a selecting ligand in shaping the murine γδ-TCR repertoire. Proc Natl Acad Sci U S A. 2018;115:1889-1894 pubmed publisher
immunoprecipitation; human; loading ...; fig sf1 immunocytochemistry; human; 1:250; loading ...; fig sf2	Chen X, Nagai Y, Zhu Z, Ruan H, Peehl D, Greene M, et al. A spliced form of CD44 expresses the unique glycan that is recognized by the prostate cancer specific antibody F77. Oncotarget. 2018;9:3631-3640 pubmed publisher
flow cytometry; mouse; loading ...; fig 5f	Koh A, Miller E, Buenrostro J, Moskowitz D, Wang J, Greenleaf W, et al. Rapid chromatin repression by Aire provides precise control of immune tolerance. Nat Immunol. 2018;19:162-172 pubmed publisher
flow cytometry; mouse; loading ...; fig 1a	Nakashima H, Alayo Q, Penaloza MacMaster P, Freeman G, Kuchroo V, Reardon D, et al. Modeling tumor immunity of mouse glioblastoma by exhausted CD8+ T cells. Sci Rep. 2018;8:208 pubmed publisher
flow cytometry; mouse; loading ...; fig 4c	Ernszt D, Banfai K, Kellermayer Z, Pap A, Lord J, Pongracz J, et al. PPARgamma Deficiency Counteracts Thymic Senescence. Front Immunol. 2017;8:1515 pubmed publisher
flow cytometry; mouse; 1:1000; loading ...; fig s1a	Yi W, Gupta S, Ricker E, Manni M, Jessberger R, Chinenov Y, et al. The mTORC1-4E-BP-eIF4E axis controls de novo Bcl6 protein synthesis in T cells and systemic autoimmunity. Nat Commun. 2017;8:254 pubmed publisher
flow cytometry; mouse; 1:400; loading ...; fig s3b	Hasan Z, Koizumi S, Sasaki D, Yamada H, Arakaki N, Fujihara Y, et al. JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 2017;8:15628 pubmed publisher
flow cytometry; mouse; loading ...; fig 4f	Daley D, Mani V, Mohan N, Akkad N, Pandian G, Savadkar S, et al. NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma. J Exp Med. 2017;214:1711-1724 pubmed publisher
flow cytometry; mouse; loading ...; fig 1c	Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
flow cytometry; mouse; loading ...; fig 3c	Daley D, Mani V, Mohan N, Akkad N, Ochi A, Heindel D, et al. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance. Nat Med. 2017;23:556-567 pubmed publisher
flow cytometry; mouse; loading ...; fig 4a	González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
flow cytometry; mouse; loading ...; fig 6b	Bhattacharyya M, Penaloza MacMaster P. T regulatory cells are critical for the maintenance, anamnestic expansion and protection elicited by vaccine-induced CD8 T cells. Immunology. 2017;151:340-348 pubmed publisher
flow cytometry; mouse; loading ...; fig 2d	Hammer A, Yang G, Friedrich J, Kovacs A, Lee D, Grave K, et al. Role of the receptor Mas in macrophage-mediated inflammation in vivo. Proc Natl Acad Sci U S A. 2016;113:14109-14114 pubmed
immunocytochemistry; human; loading ...; fig 3g	Jung Y, Riven I, Feigelson S, Kartvelishvily E, Tohya K, Miyasaka M, et al. Three-dimensional localization of T-cell receptors in relation to microvilli using a combination of superresolution microscopies. Proc Natl Acad Sci U S A. 2016;113:E5916-E5924 pubmed
flow cytometry; mouse; loading ...; fig 2e	Chopra M, Biehl M, Steinfatt T, Brandl A, Kums J, Amich J, et al. Exogenous TNFR2 activation protects from acute GvHD via host T reg cell expansion. J Exp Med. 2016;213:1881-900 pubmed publisher
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product information

Antigen :

CD44

Apps. Abbrev. :

FC, ICC, SB

Cat # :

103017

Clone :

IM7

Item :

Alexa Fluor® 647 anti-mouse/human CD44

Isotype :

Rat IgG2b, κ

Other Names :

Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24

Size :

25 μg

Price (USD) :

101 USD

Reactivity :

Mouse, Human

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

A647

Immunogen :

Dexamethasone-induced myeloid leukemia M1 cells

Application Notes :

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186^20. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity^1, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.;

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