product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
PE anti-mouse/human CD44
catalog :
103007
quantity :
50 μg
price :
56 USD
clonality :
monoclonal
host :
rat
conjugate :
PE
clone name :
IM7
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry
more info or order :
citations: 113
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 2a
Susukida T, Kuwahara S, Song B, Kazaoka A, Aoki S, Ito K. Regulation of the immune tolerance system determines the susceptibility to HLA-mediated abacavir-induced skin toxicity. Commun Biol. 2021;4:1137 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Reis M, Willis G, Fernandez Gonzalez A, Yeung V, Taglauer E, Magaletta M, et al. Mesenchymal Stromal Cell-Derived Extracellular Vesicles Restore Thymic Architecture and T Cell Function Disrupted by Neonatal Hyperoxia. Front Immunol. 2021;12:640595 pubmed publisher
  • flow cytometry; human; loading ...
Liao T, Lin C, Jiang J, Yang S, Teng H, Yang M. Harnessing stemness and PD-L1 expression by AT-rich interaction domain-containing protein 3B in colorectal cancer. Theranostics. 2020;10:6095-6112 pubmed publisher
  • flow cytometry; mouse; loading ...
Zhu M, Ma Y, Tan K, Zhang L, Wang Z, Li Y, et al. Thalidomide with blockade of co-stimulatory molecules prolongs the survival of alloantigen-primed mice with cardiac allografts. BMC Immunol. 2020;21:19 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s1a
Angenendt A, Steiner R, Knörck A, Schwär G, Konrad M, Krause E, et al. Orai, STIM, and PMCA contribute to reduced calcium signal generation in CD8+ T cells of elderly mice. Aging (Albany NY). 2020;12:3266-3286 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2e
Faliti C, Gualtierotti R, Rottoli E, Gerosa M, Perruzza L, Romagnani A, et al. P2X7 receptor restrains pathogenic Tfh cell generation in systemic lupus erythematosus. J Exp Med. 2019;216:317-336 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 4c
Ernszt D, Banfai K, Kellermayer Z, Pap A, Lord J, Pongracz J, et al. PPARgamma Deficiency Counteracts Thymic Senescence. Front Immunol. 2017;8:1515 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2d
Hammer A, Yang G, Friedrich J, Kovacs A, Lee D, Grave K, et al. Role of the receptor Mas in macrophage-mediated inflammation in vivo. Proc Natl Acad Sci U S A. 2016;113:14109-14114 pubmed
  • flow cytometry; mouse; fig 6f
Serr I, Fürst R, Ott V, Scherm M, Nikolaev A, Gökmen F, et al. miRNA92a targets KLF2 and the phosphatase PTEN signaling to promote human T follicular helper precursors in T1D islet autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6659-E6668 pubmed
  • flow cytometry; mouse; loading ...; fig 2c
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
  • flow cytometry; mouse; fig 5
Mikyšková R, Štěpánek I, Indrová M, Bieblová J, Šímová J, Truxová I, et al. Dendritic cells pulsed with tumor cells killed by high hydrostatic pressure induce strong immune responses and display therapeutic effects both in murine TC-1 and TRAMP-C2 tumors when combined with docetaxel chemotherapy. Int J Oncol. 2016;48:953-64 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 3a
Ngiow S, Young A, Jacquelot N, Yamazaki T, Enot D, Zitvogel L, et al. A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1. Cancer Res. 2015;75:3800-11 pubmed publisher
  • flow cytometry; mouse
Olguín J, Fernández J, Salinas N, Juárez I, Rodriguez Sosa M, Campuzano J, et al. Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated Th1 immune response. Microbes Infect. 2015;17:586-95 pubmed publisher
  • flow cytometry; mouse
Kitagawa K, Shibata K, Matsumoto A, Matsumoto M, Ohhata T, Nakayama K, et al. Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development. Mol Cell Biol. 2014;34:2732-44 pubmed
  • immunocytochemistry; human; 1:200; loading ...; fig st13
Hwang W, Jiang J, Yang S, Huang T, Lan H, Teng H, et al. MicroRNA-146a directs the symmetric division of Snail-dominant colorectal cancer stem cells. Nat Cell Biol. 2014;16:268-80 pubmed publisher
Volpedo G, Pacheco Fernández T, Holcomb E, Zhang W, Lypaczewski P, Cox B, et al. Centrin-deficient Leishmania mexicana confers protection against New World cutaneous leishmaniasis. NPJ Vaccines. 2022;7:32 pubmed publisher
Chen L, Zhou C, Chen Q, Shang J, Liu Z, Guo Y, et al. Oncolytic Zika virus promotes intratumoral T cell infiltration and improves immunotherapy efficacy in glioblastoma. Mol Ther Oncolytics. 2022;24:522-534 pubmed publisher
Wu J, Li G, Li D, Zhou J, Dong Z, Jiang P. Detection of CD8+ T cell-mediated immune responses to bacterial infection in mice. STAR Protoc. 2021;2:101022 pubmed publisher
Liu X, Ye N, Liu S, Guan J, Deng Q, Zhang Z, et al. Hyperbaric Oxygen Boosts PD-1 Antibody Delivery and T Cell Infiltration for Augmented Immune Responses Against Solid Tumors. Adv Sci (Weinh). 2021;8:e2100233 pubmed publisher
Zhuang Z, Lai X, Sun J, Chen Z, Zhang Z, Dai J, et al. Mapping and role of T cell response in SARS-CoV-2-infected mice. J Exp Med. 2021;218: pubmed publisher
Chakraborty M, Chu K, Shrestha A, Revelo X, Zhang X, Gold M, et al. Mechanical Stiffness Controls Dendritic Cell Metabolism and Function. Cell Rep. 2021;34:108609 pubmed publisher
Lv H, Lv G, Chen C, Zong Q, Jiang G, Ye D, et al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Cell Metab. 2021;33:110-127.e5 pubmed publisher
Ringel A, Drijvers J, Baker G, Catozzi A, García Cañaveras J, Gassaway B, et al. Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor Immunity. Cell. 2020;183:1848-1866.e26 pubmed publisher
Argüello R, Combes A, Char R, Gigan J, Baaziz A, Bousiquot E, et al. SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution. Cell Metab. 2020;32:1063-1075.e7 pubmed publisher
Byun J, Park M, Lee S, Yun J, Lee J, Kim J, et al. Inhibition of Glutamine Utilization Synergizes with Immune Checkpoint Inhibitor to Promote Antitumor Immunity. Mol Cell. 2020;80:592-606.e8 pubmed publisher
Fan C, Feng J, Tang C, Zhang Z, Feng Y, Duan W, et al. Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage. Stem Cell Res Ther. 2020;11:442 pubmed publisher
Browning L, Miller C, Kuczma M, Pietrzak M, Jing Y, Rempala G, et al. Bone Morphogenic Proteins Are Immunoregulatory Cytokines Controlling FOXP3+ Treg Cells. Cell Rep. 2020;33:108219 pubmed publisher
Shapiro M, Yeh W, Longfield J, Gallagher J, Infante C, Wellford S, et al. CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell Activation. Front Immunol. 2020;11:2180 pubmed publisher
Hassan A, Kafai N, Dmitriev I, Fox J, Smith B, Harvey I, et al. A Single-Dose Intranasal ChAd Vaccine Protects Upper and Lower Respiratory Tracts against SARS-CoV-2. Cell. 2020;183:169-184.e13 pubmed publisher
Yi X, Zhou H, Chao Y, Xiong S, Zhong J, Chai Z, et al. Bacteria-triggered tumor-specific thrombosis to enable potent photothermal immunotherapy of cancer. Sci Adv. 2020;6:eaba3546 pubmed publisher
Fu X, Luo H, Zheng Y, Wang S, Zhong Z, Wang Y, et al. CTLA-4 immunotherapy exposes differences in immune response along with different tumor progression in colorectal cancer. Aging (Albany NY). 2020;12:15656-15669 pubmed publisher
Zhang N, Li X, Deng Y, Zhao H, Huang Y, Yang G, et al. A Thermostable mRNA Vaccine against COVID-19. Cell. 2020;182:1271-1283.e16 pubmed publisher
Chulpanova D, Solovyeva V, James V, Arkhipova S, Gomzikova M, Garanina E, et al. Human Mesenchymal Stem Cells Overexpressing Interleukin 2 Can Suppress Proliferation of Neuroblastoma Cells in Co-Culture and Activate Mononuclear Cells In Vitro. Bioengineering (Basel). 2020;7: pubmed publisher
Yermanos A, Sandu I, Pedrioli A, Borsa M, Wagen F, Oetiker N, et al. Profiling Virus-Specific Tcf1+ T Cell Repertoires During Acute and Chronic Viral Infection. Front Immunol. 2020;11:986 pubmed publisher
Shi Y, Kang X, Wang Y, Bian X, He G, Zhou M, et al. Exosomes Derived from Bone Marrow Stromal Cells (BMSCs) Enhance Tendon-Bone Healing by Regulating Macrophage Polarization. Med Sci Monit. 2020;26:e923328 pubmed publisher
Isakova Sivak I, Matyushenko V, Stepanova E, Matushkina A, Kotomina T, Mezhenskaya D, et al. Recombinant Live Attenuated Influenza Vaccine Viruses Carrying Conserved T-cell Epitopes of Human Adenoviruses Induce Functional Cytotoxic T-Cell Responses and Protect Mice against Both Infections. Vaccines (Basel). 2020;8: pubmed publisher
Lin W, Xu Y, Chen X, Liu J, Weng Y, Zhuang Q, et al. Radiation-induced small extracellular vesicles as "carriages" promote tumor antigen release and trigger antitumor immunity. Theranostics. 2020;10:4871-4884 pubmed publisher
Yeung F, Chen Y, Lin J, Leung J, McCauley C, Devlin J, et al. Altered Immunity of Laboratory Mice in the Natural Environment Is Associated with Fungal Colonization. Cell Host Microbe. 2020;27:809-822.e6 pubmed publisher
Girón Pérez D, Vadillo E, Schnoor M, Santos Argumedo L. Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes. J Cell Sci. 2020;133: pubmed publisher
Ge C, Monk I, Pizzolla A, Wang N, Bedford J, Stinear T, et al. Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment. Cell Rep. 2019;29:4236-4244.e3 pubmed publisher
Shao B, Yago T, Panicker S, Zhang N, Liu Z, McEver R. Th1 Cells Rolling on Selectins Trigger DAP12-Dependent Signals That Activate Integrin αLβ2. J Immunol. 2020;204:37-48 pubmed publisher
Upasani V, Vo H, Ung S, Heng S, Laurent D, Choeung R, et al. Impaired Antibody-Independent Immune Response of B Cells in Patients With Acute Dengue Infection. Front Immunol. 2019;10:2500 pubmed publisher
Kong T, Ahn R, Yang K, Zhu X, Fu Z, Morin G, et al. CD44 Promotes PD-L1 Expression and Its Tumor-Intrinsic Function in Breast and Lung Cancers. Cancer Res. 2020;80:444-457 pubmed publisher
Santos C, Lapi E, Martínez de Villarreal J, Álvaro Espinosa L, Fernández Barral A, Barbachano A, et al. Urothelial organoids originating from Cd49fhigh mouse stem cells display Notch-dependent differentiation capacity. Nat Commun. 2019;10:4407 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Miller B, Yates K, Gillis J, et al. PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019;20:1335-1347 pubmed publisher
Yang C, Siebert J, Burns R, Gerbec Z, Bonacci B, Rymaszewski A, et al. Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome. Nat Commun. 2019;10:3931 pubmed publisher
Zhang H, Alsaleh G, Feltham J, Sun Y, Napolitano G, Riffelmacher T, et al. Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Mol Cell. 2019;76:110-125.e9 pubmed publisher
Sasaki K, Himeno A, Nakagawa T, Sasaki Y, Kiyonari H, Iwai K. Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death. Nat Commun. 2019;10:3878 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Zhang Z, Zhu L, Feng P, Tan Y, Zhang B, Gao E, et al. C1q/tumor necrosis factor-related protein-3-engineered mesenchymal stromal cells attenuate cardiac impairment in mice with myocardial infarction. Cell Death Dis. 2019;10:530 pubmed publisher
Bhattacharjee S, Mejías Luque R, Loffredo Verde E, Toska A, Flossdorf M, Gerhard M, et al. Concomitant Infection of S. mansoni and H. pylori Promotes Promiscuity of Antigen-Experienced Cells and Primes the Liver for a Lower Fibrotic Response. Cell Rep. 2019;28:231-244.e5 pubmed publisher
Yang B, Wang K, Wan S, Liang Y, Yuan X, Dong Y, et al. TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases. Cell Rep. 2019;27:3629-3645.e6 pubmed publisher
Timilshina M, You Z, Lacher S, Acharya S, Jiang L, Kang Y, et al. Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of Treg Cells. Cell Rep. 2019;27:2948-2961.e7 pubmed publisher
Qi X, Li F, Wu Y, Cheng C, Han P, Wang J, et al. Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity. Nat Commun. 2019;10:2141 pubmed publisher
Yue X, Lio C, Samaniego Castruita D, Li X, Rao A. Loss of TET2 and TET3 in regulatory T cells unleashes effector function. Nat Commun. 2019;10:2011 pubmed publisher
Hu Q, Sun W, Wang J, Ruan H, Zhang X, Ye Y, et al. Conjugation of haematopoietic stem cells and platelets decorated with anti-PD-1 antibodies augments anti-leukaemia efficacy. Nat Biomed Eng. 2018;2:831-840 pubmed publisher
LaFleur M, Nguyen T, Coxe M, Yates K, Trombley J, Weiss S, et al. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nat Commun. 2019;10:1668 pubmed publisher
Miska J, Lee Chang C, Rashidi A, Muroski M, Chang A, Lopez Rosas A, et al. HIF-1α Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma. Cell Rep. 2019;27:226-237.e4 pubmed publisher
Du L, Lin L, Li Q, Liu K, Huang Y, Wang X, et al. IGF-2 Preprograms Maturing Macrophages to Acquire Oxidative Phosphorylation-Dependent Anti-inflammatory Properties. Cell Metab. 2019;29:1363-1375.e8 pubmed publisher
Lu H, Ju D, Yang G, Zhu L, Yang X, Li J, et al. Targeting cancer stem cell signature gene SMOC-2 Overcomes chemoresistance and inhibits cell proliferation of endometrial carcinoma. EBioMedicine. 2019;40:276-289 pubmed publisher
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Yip H, Tan C, Hirokawa Y, Burgess A. Colon organoid formation and cryptogenesis are stimulated by growth factors secreted from myofibroblasts. PLoS ONE. 2018;13:e0199412 pubmed publisher
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Hammer A, Schliep A, Jörg S, Haghikia A, Gold R, Kleinewietfeld M, et al. Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation. J Neuroinflammation. 2017;14:184 pubmed publisher
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Barber A, Sentman C. NKG2D receptor regulates human effector T-cell cytokine production. Blood. 2011;117:6571-81 pubmed publisher
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Morley S, Weber K, Kao H, Allen P. Protein kinase C-theta is required for efficient positive selection. J Immunol. 2008;181:4696-708 pubmed
Wang X, Lan Y, He W, Zhang L, Yao H, Hou C, et al. Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos. Blood. 2008;111:2436-43 pubmed
Kenna T, Thomas R, Steptoe R. Steady-state dendritic cells expressing cognate antigen terminate memory CD8+ T-cell responses. Blood. 2008;111:2091-100 pubmed
Gui J, Zhu X, Dohkan J, Cheng L, Barnes P, Su D. The aged thymus shows normal recruitment of lymphohematopoietic progenitors but has defects in thymic epithelial cells. Int Immunol. 2007;19:1201-11 pubmed
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product information
Antigen :
CD44
Apps. Abbrev. :
FC
Cat # :
103007
Clone :
IM7
Item :
PE anti-mouse/human CD44
Isotype :
Rat IgG2b, κ
Other Names :
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Size :
50 μg
Price (USD) :
56 USD
Reactivity :
Mouse, Human
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PE
Immunogen :
Dexamethasone-induced myeloid leukemia M1 cells
Application Notes :
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186^20. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity^1, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.;
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