Ultra-LEAF™ Purified anti-mouse CD40 | BioLegend 102812 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Ultra-LEAF™ Purified anti-mouse CD40

catalog :

102812

quantity :

1 mg

price :

545 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

1C10

reactivity :

mouse

application :

neutralization, immunoprecipitation, flow cytometry

more info or order :

BioLegend product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 1a	Yokota Nakatsuma A, Ohoka Y, Takeuchi H, Song S, Iwata M. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties. Sci Rep. 2016;6:37914 pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Khameneh H, Ho A, Spreafico R, Derks H, Quek H, Mortellaro A. The Syk-NFAT-IL-2 Pathway in Dendritic Cells Is Required for Optimal Sterile Immunity Elicited by Alum Adjuvants. J Immunol. 2017;198:196-204 pubmed
flow cytometry; mouse; loading ...; fig s4e	Klotz L, Kuzmanov I, Hucke S, Gross C, Posevitz V, Dreykluft A, et al. B7-H1 shapes T-cell-mediated brain endothelial cell dysfunction and regional encephalitogenicity in spontaneous CNS autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6182-E6191 pubmed
flow cytometry; mouse; fig 3	LUCAS B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, et al. CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice. Eur J Immunol. 2015;45:574-83 pubmed publisher
flow cytometry; mouse; fig 2	McDonnell A, Lesterhuis W, Khong A, Nowak A, Lake R, Currie A, et al. Tumor-infiltrating dendritic cells exhibit defective cross-presentation of tumor antigens, but is reversed by chemotherapy. Eur J Immunol. 2015;45:49-59 pubmed publisher
	Arensman M, Yang X, Zhong W, Bisulco S, Upeslacis E, Rosfjord E, et al. Anti-tumor immunity influences cancer cell reliance upon ATG7. Oncoimmunology. 2020;9:1800162 pubmed publisher
	Fox A, Dutt T, Karger B, Obregón Henao A, Anderson G, Henao Tamayo M. Acquisition of High-Quality Spectral Flow Cytometry Data. Curr Protoc Cytom. 2020;93:e74 pubmed publisher
	Xie Y, Peng J, Pang J, Guo K, Zhang L, Yin S, et al. Biglycan regulates neuroinflammation by promoting M1 microglial activation in early brain injury after experimental subarachnoid hemorrhage. J Neurochem. 2020;152:368-380 pubmed publisher
	Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
	Ponomarev E, Shriver L, Maresz K, Pedras Vasconcelos J, Verthelyi D, Dittel B. GM-CSF production by autoreactive T cells is required for the activation of microglial cells and the onset of experimental autoimmune encephalomyelitis. J Immunol. 2007;178:39-48 pubmed

product information

Antigen :

CD40

Apps. Abbrev. :

FC, Costim, Block, IP

Cat # :

102812

Clone :

1C10

Item :

Ultra-LEAF™ Purified anti-mouse CD40

Isotype :

Rat IgG2a, κ

Other Names :

Bp50, TNFRSF5

Size :

1 mg

Price (USD) :

545 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

ULEAF

Application Notes :

The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking^5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation^1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102810). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102812) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;

more info or order :

BioLegend product webpage