Purified anti-mouse CD40 | BioLegend 102802 product information

product summary

company name :

BioLegend

other brands :

Babco, Signet, Sternberger Monoclonals, Senetek, Covance

product type :

antibody

product name :

Purified anti-mouse CD40

catalog :

102802

quantity :

500 μg

price :

235 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

1C10

reactivity :

mouse

application :

neutralization, immunoprecipitation, flow cytometry

more info or order :

BioLegend product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; fig 1a	Yokota Nakatsuma A, Ohoka Y, Takeuchi H, Song S, Iwata M. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties. Sci Rep. 2016;6:37914 pubmed publisher
flow cytometry; mouse; loading ...; fig 6c	Khameneh H, Ho A, Spreafico R, Derks H, Quek H, Mortellaro A. The Syk-NFAT-IL-2 Pathway in Dendritic Cells Is Required for Optimal Sterile Immunity Elicited by Alum Adjuvants. J Immunol. 2017;198:196-204 pubmed
flow cytometry; mouse; loading ...; fig s4e	Klotz L, Kuzmanov I, Hucke S, Gross C, Posevitz V, Dreykluft A, et al. B7-H1 shapes T-cell-mediated brain endothelial cell dysfunction and regional encephalitogenicity in spontaneous CNS autoimmunity. Proc Natl Acad Sci U S A. 2016;113:E6182-E6191 pubmed
flow cytometry; mouse; fig 3	LUCAS B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, et al. CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice. Eur J Immunol. 2015;45:574-83 pubmed publisher
flow cytometry; mouse; fig 2	McDonnell A, Lesterhuis W, Khong A, Nowak A, Lake R, Currie A, et al. Tumor-infiltrating dendritic cells exhibit defective cross-presentation of tumor antigens, but is reversed by chemotherapy. Eur J Immunol. 2015;45:49-59 pubmed publisher
	Sakaguchi T, Okumura R, Ono C, Okuzaki D, Kawai T, Okochi Y, et al. TRPM5 Negatively Regulates Calcium-Dependent Responses in Lipopolysaccharide-Stimulated B Lymphocytes. Cell Rep. 2020;31:107755 pubmed publisher
	Lu X, Chu C, Fang T, Rayon Estrada V, Fang F, Patke A, et al. MTA2/NuRD Regulates B Cell Development and Cooperates with OCA-B in Controlling the Pre-B to Immature B Cell Transition. Cell Rep. 2019;28:472-485.e5 pubmed publisher
	Obieglo K, Costain A, Webb L, Ozir Fazalalikhan A, Brown S, MacDonald A, et al. Type I interferons provide additive signals for murine regulatory B cell induction by Schistosoma mansoni eggs. Eur J Immunol. 2019;49:1226-1234 pubmed publisher
	Graf R, Li X, Chu V, Rajewsky K. sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing. Cell Rep. 2019;26:1098-1103.e3 pubmed publisher
	Hoves S, Ooi C, Wolter C, Sade H, Bissinger S, Schmittnaegel M, et al. Rapid activation of tumor-associated macrophages boosts preexisting tumor immunity. J Exp Med. 2018;215:859-876 pubmed publisher
	Le Q, Maizels N. Cell Cycle Regulates Nuclear Stability of AID and Determines the Cellular Response to AID. PLoS Genet. 2015;11:e1005411 pubmed publisher
	Rastelli J, Hömig Hölzel C, Seagal J, Muller W, Hermann A, Rajewsky K, et al. LMP1 signaling can replace CD40 signaling in B cells in vivo and has unique features of inducing class-switch recombination to IgG1. Blood. 2008;111:1448-55 pubmed
	Zhang P, Sun D, Ke Y, Kaplan H, Shao H. The net effect of costimulatory blockers is dependent on the subset and activation status of the autoreactive T cells. J Immunol. 2007;178:474-9 pubmed
	Ashman R, Goeken J, Drahos J, Lenert P. Sequence requirements for oligodeoxyribonucleotide inhibitory activity. Int Immunol. 2005;17:411-20 pubmed

product information

Antigen :

CD40

Apps. Abbrev. :

FC, Costim, Block, IP

Cat # :

102802

Clone :

1C10

Item :

Purified anti-mouse CD40

Isotype :

Rat IgG2a, κ

Other Names :

Bp50, TNFRSF5

Size :

500 μg

Price (USD) :

235 USD

Reactivity :

Mouse

Clonality :

Monoclonal

Host :

Rat

Conjugate/Tag/Label :

PURE

Application Notes :

The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking^5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation^1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102810). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102812) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).;

more info or order :

BioLegend product webpage