product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 594 anti-mouse CD31
catalog :
102520
quantity :
100 μg
price :
253 USD
clonality :
monoclonal
host :
rat
conjugate :
Alexa Fluor 594
clone name :
MEC13.3
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 41
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1c
Duan J, Zhou Z, Ruan B, Fang Z, Ding J, Liu J, et al. Notch-Regulated c-Kit-Positive Liver Sinusoidal Endothelial Cells Contribute to Liver Zonation and Regeneration. Cell Mol Gastroenterol Hepatol. 2022;13:1741-1756 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5e
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5f
Thanabalasuriar A, Chiang A, Morehouse C, Camara M, Hawkins S, Keller A, et al. PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Sci Adv. 2021;7: pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 2a
Chelvanambi M, Fecek R, Taylor J, Storkus W. STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment. J Immunother Cancer. 2021;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Xanthis I, Souilhol C, Serbanovic Canic J, Roddie H, Kalli A, Fragiadaki M, et al. β1 integrin is a sensor of blood flow direction. J Cell Sci. 2019;132: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Cunin P, Bouslama R, Machlus K, Martínez Bonet M, Lee P, Wactor A, et al. Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig ev1b
Gurevich D, Severn C, Twomey C, Greenhough A, Cash J, Toye A, et al. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1f
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Singh P, Hoggatt J, Kamocka M, Mohammad K, Saunders M, Li H, et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest. 2017;127:4527-4540 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
Baba T, Miyazaki D, Inata K, Uotani R, Miyake H, Sasaki S, et al. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration. elife. 2020;9: pubmed publisher
Lang J, Toulmin S, Brida K, Eisenlohr L, Davidson B. Standard screening methods underreport AAV-mediated transduction and gene editing. Nat Commun. 2019;10:3415 pubmed publisher
Baptista A, Gola A, Huang Y, Milanez Almeida P, Torabi Parizi P, Urban J, et al. The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4+ T Cell Peripheralization to Promote Effective Adaptive Immunity. Immunity. 2019;50:1188-1201.e6 pubmed publisher
Denton A, Innocentin S, Carr E, Bradford B, Lafouresse F, Mabbott N, et al. Type I interferon induces CXCL13 to support ectopic germinal center formation. J Exp Med. 2019;216:621-637 pubmed publisher
Kolb R, Kluz P, Tan Z, Borcherding N, Bormann N, Vishwakarma A, et al. Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4. Oncogene. 2019;38:2351-2363 pubmed publisher
Wu F, Xu P, Chow A, Man S, Kruger J, Khan K, et al. Pre- and post-operative anti-PD-L1 plus anti-angiogenic therapies in mouse breast or renal cancer models of micro- or macro-metastatic disease. Br J Cancer. 2019;120:196-206 pubmed publisher
Welz M, Eickhoff S, Abdullah Z, Trebicka J, Gartlan K, Spicer J, et al. Perforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis. Nat Commun. 2018;9:4805 pubmed publisher
Bousquenaud M, Fico F, Solinas G, Ruegg C, Santamaria Martínez A. Obesity promotes the expansion of metastasis-initiating cells in breast cancer. Breast Cancer Res. 2018;20:104 pubmed publisher
Wang H, D Souza C, Lim X, Kostenko L, Pediongco T, Eckle S, et al. MAIT cells protect against pulmonary Legionella longbeachae infection. Nat Commun. 2018;9:3350 pubmed publisher
Maeda K, Otomo K, Yoshida N, Abu Asab M, Ichinose K, Nishino T, et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. J Clin Invest. 2018;128:3445-3459 pubmed publisher
Vandoorne K, Rohde D, Kim H, Courties G, Wojtkiewicz G, Honold L, et al. Imaging the Vascular Bone Marrow Niche During Inflammatory Stress. Circ Res. 2018;: pubmed publisher
Zimmerli D, Cecconi V, Valenta T, Hausmann G, Cantù C, Restivo G, et al. WNT ligands control initiation and progression of human papillomavirus-driven squamous cell carcinoma. Oncogene. 2018;37:3753-3762 pubmed publisher
Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
Kim I, Kim K, Lee E, Oh D, Park C, Park S, et al. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality. J Exp Med. 2018;215:963-983 pubmed publisher
Böttcher J, Bonavita E, Chakravarty P, Blees H, Cabeza Cabrerizo M, Sammicheli S, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control. Cell. 2018;172:1022-1037.e14 pubmed publisher
Zhang Y, Daubel N, Stritt S, Mäkinen T. Transient loss of venous integrity during developmental vascular remodeling leads to red blood cell extravasation and clearance by lymphatic vessels. Development. 2018;145: pubmed publisher
Engblom C, Pfirschke C, Zilionis R, da Silva Martins J, Bos S, Courties G, et al. Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils. Science. 2017;358: pubmed publisher
Kanemaru H, Yamane F, Fukushima K, Matsuki T, Kawasaki T, Ebina I, et al. Antitumor effect of Batf2 through IL-12 p40 up-regulation in tumor-associated macrophages. Proc Natl Acad Sci U S A. 2017;114:E7331-E7340 pubmed publisher
Thiriot A, Perdomo C, Cheng G, Novitzky Basso I, McArdle S, Kishimoto J, et al. Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues. BMC Biol. 2017;15:45 pubmed publisher
Kammertoens T, Friese C, Arina A, Idel C, Briesemeister D, Rothe M, et al. Tumour ischaemia by interferon-? resembles physiological blood vessel regression. Nature. 2017;545:98-102 pubmed publisher
Tian L, Goldstein A, Wang H, Ching Lo H, Sun Kim I, Welte T, et al. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming. Nature. 2017;544:250-254 pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Mueller A, van Velthoven C, Fukumoto K, Cheung T, Rando T. Intronic polyadenylation of PDGFR? in resident stem cells attenuates muscle fibrosis. Nature. 2016;540:276-279 pubmed publisher
Liu Z, Tian R, Li Y, Zhang L, Shao H, Yang C, et al. SDF-1?-induced dual pairs of E-selectin/ligand mediate endothelial progenitor cell homing to critical ischemia. Sci Rep. 2016;6:34416 pubmed publisher
Kumar D, Ristow L, Shi M, Mukherjee P, Caine J, Lee W, et al. Intravital Imaging of Vascular Transmigration by the Lyme Spirochete: Requirement for the Integrin Binding Residues of the B. burgdorferi P66 Protein. PLoS Pathog. 2015;11:e1005333 pubmed publisher
Ramos Perez W, Fang V, Escalante Alcalde D, Cammer M, Schwab S. A map of the distribution of sphingosine 1-phosphate in the spleen. Nat Immunol. 2015;16:1245-52 pubmed publisher
Giacomini C, Ferrari G, Bignami F, Rama P. Alkali burn versus suture-induced corneal neovascularization in C57BL/6 mice: an overview of two common animal models of corneal neovascularization. Exp Eye Res. 2014;121:1-4 pubmed publisher
Leung V, Yun S, Botto M, Mason J, Malik T, Song W, et al. Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice. Am J Pathol. 2009;175:1757-67 pubmed publisher
product information
Antigen :
CD31
Apps. Abbrev. :
FC, IHC-F, 3D IHC, SB
Cat # :
102520
Clone :
MEC13.3
Item :
Alexa Fluor® 594 anti-mouse CD31
Isotype :
Rat IgG2a, κ
Other Names :
PECAM-1, EndoCAM
Size :
100 μg
Price (USD) :
253 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A594
Immunogen :
Polyoma middle T transformed EC line tEnd.1
Application Notes :
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.^8 Additional reported applications (in the relevant formats) include: immunoprecipitation^1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^12,13.Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA