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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 647 anti-mouse CD31
catalog :
102516
quantity :
100 μg
price :
229 USD
clonality :
monoclonal
host :
rat
conjugate :
AF647
clone name :
MEC13.3
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 81
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; 1:200; loading ...; fig 3f, s8e
Omatsu Y, Aiba S, Maeta T, Higaki K, Aoki K, Watanabe H, et al. Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells. Nat Commun. 2022;13:2654 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1c
Duan J, Zhou Z, Ruan B, Fang Z, Ding J, Liu J, et al. Notch-Regulated c-Kit-Positive Liver Sinusoidal Endothelial Cells Contribute to Liver Zonation and Regeneration. Cell Mol Gastroenterol Hepatol. 2022;13:1741-1756 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 2c
Götz P, Braumandl A, Kübler M, Kumaraswami K, Ishikawa Ankerhold H, Lasch M, et al. C3 Deficiency Leads to Increased Angiogenesis and Elevated Pro-Angiogenic Leukocyte Recruitment in Ischemic Muscle Tissue. Int J Mol Sci. 2021;22: pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5e
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5f
Thanabalasuriar A, Chiang A, Morehouse C, Camara M, Hawkins S, Keller A, et al. PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Sci Adv. 2021;7: pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 2a
Chelvanambi M, Fecek R, Taylor J, Storkus W. STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment. J Immunother Cancer. 2021;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig 2s1c
Baba T, Miyazaki D, Inata K, Uotani R, Miyake H, Sasaki S, et al. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration. elife. 2020;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 1l
Grüneboom A, Hawwari I, Weidner D, Culemann S, Müller S, Henneberg S, et al. A network of trans-cortical capillaries as mainstay for blood circulation in long bones. Nat Metab. 2019;1:236-250 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2f
Collins N, Han S, Enamorado M, Link V, Huang B, Moseman E, et al. The Bone Marrow Protects and Optimizes Immunological Memory during Dietary Restriction. Cell. 2019;178:1088-1101.e15 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Xanthis I, Souilhol C, Serbanovic Canic J, Roddie H, Kalli A, Fragiadaki M, et al. β1 integrin is a sensor of blood flow direction. J Cell Sci. 2019;132: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Cunin P, Bouslama R, Machlus K, Martínez Bonet M, Lee P, Wactor A, et al. Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig ev1b
Gurevich D, Severn C, Twomey C, Greenhough A, Cash J, Toye A, et al. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1f
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Singh P, Hoggatt J, Kamocka M, Mohammad K, Saunders M, Li H, et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest. 2017;127:4527-4540 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
Sakamoto K, Goel S, Funakoshi A, Honda T, Nagao K. Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells. STAR Protoc. 2022;3:101052 pubmed publisher
Wang S, Ren D, Arkoun B, Kaushik A, Matherat G, Lecluse Y, et al. Lyl-1 regulates primitive macrophages and microglia development. Commun Biol. 2021;4:1382 pubmed publisher
Devi S, Alexandre Y, Loi J, Gillis R, Ghazanfari N, Creed S, et al. Adrenergic regulation of the vasculature impairs leukocyte interstitial migration and suppresses immune responses. Immunity. 2021;54:1219-1230.e7 pubmed publisher
Friščić J, Böttcher M, Reinwald C, Bruns H, Wirth B, Popp S, et al. The complement system drives local inflammatory tissue priming by metabolic reprogramming of synovial fibroblasts. Immunity. 2021;54:1002-1021.e10 pubmed publisher
Rustenhoven J, Drieu A, Mamuladze T, de Lima K, Dykstra T, Wall M, et al. Functional characterization of the dural sinuses as a neuroimmune interface. Cell. 2021;184:1000-1016.e27 pubmed publisher
Stacy A, Andrade Oliveira V, McCulloch J, Hild B, Oh J, Pérez Chaparro P, et al. Infection trains the host for microbiota-enhanced resistance to pathogens. Cell. 2021;184:615-627.e17 pubmed publisher
Tsukada Y, Muramatsu F, Hayashi Y, Inagaki C, Su H, Iba T, et al. An in vivo model allowing continuous observation of human vascular formation in the same animal over time. Sci Rep. 2021;11:745 pubmed publisher
Shannon J, Vrba S, Reynoso G, Wynne Jones E, Kamenyeva O, Malo C, et al. Group 1 innate lymphoid-cell-derived interferon-γ maintains anti-viral vigilance in the mucosal epithelium. Immunity. 2021;54:276-290.e5 pubmed publisher
Evren E, Ringqvist E, Tripathi K, Sleiers N, Rives I, Alisjahbana A, et al. Distinct developmental pathways from blood monocytes generate human lung macrophage diversity. Immunity. 2021;54:259-275.e7 pubmed publisher
Neufert C, Heichler C, Brabletz T, Scheibe K, Boonsanay V, Greten F, et al. Inducible mouse models of colon cancer for the analysis of sporadic and inflammation-driven tumor progression and lymph node metastasis. Nat Protoc. 2021;16:61-85 pubmed publisher
Alexandre Y, Devi S, Park S, Mackay L, Heath W, Mueller S. Systemic Inflammation Suppresses Lymphoid Tissue Remodeling and B Cell Immunity during Concomitant Local Infection. Cell Rep. 2020;33:108567 pubmed publisher
Kumaraswami K, Salei N, Beck S, Rambichler S, Kluever A, Lasch M, et al. A Simple and Effective Flow Cytometry-Based Method for Identification and Quantification of Tissue Infiltrated Leukocyte Subpopulations in a Mouse Model of Peripheral Arterial Disease. Int J Mol Sci. 2020;21: pubmed publisher
Jiang Y, Wang H, Peng J, Zhu Y, Zhang H, Tang F, et al. Multinucleated polyploid cardiomyocytes undergo an enhanced adaptability to hypoxia via mitophagy. J Mol Cell Cardiol. 2020;138:115-135 pubmed publisher
Hoyer F, Naxerova K, Schloss M, Hulsmans M, Nair A, Dutta P, et al. Tissue-Specific Macrophage Responses to Remote Injury Impact the Outcome of Subsequent Local Immune Challenge. Immunity. 2019;51:899-914.e7 pubmed publisher
Xu M, Xu H, Lin Y, Sun X, Wang L, Fang Z, et al. LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis. Cell. 2019;178:1478-1492.e20 pubmed publisher
Choudhury S, Babes L, Rahn J, Ahn B, Goring K, King J, et al. Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver. Cell. 2019;178:1205-1221.e17 pubmed publisher
Lang J, Toulmin S, Brida K, Eisenlohr L, Davidson B. Standard screening methods underreport AAV-mediated transduction and gene editing. Nat Commun. 2019;10:3415 pubmed publisher
Severe N, Karabacak N, Gustafsson K, Baryawno N, Courties G, Kfoury Y, et al. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping. Cell Stem Cell. 2019;25:570-583.e7 pubmed publisher
Mariani S, Li Z, Rice S, Krieg C, Fragkogianni S, Robinson M, et al. Pro-inflammatory Aorta-Associated Macrophages Are Involved in Embryonic Development of Hematopoietic Stem Cells. Immunity. 2019;: pubmed publisher
Merz S, Korste S, Bornemann L, Michel L, Stock P, Squire A, et al. Contemporaneous 3D characterization of acute and chronic myocardial I/R injury and response. Nat Commun. 2019;10:2312 pubmed publisher
Silva C, Sampaio Pinto V, Andrade S, Rodrigues I, Costa R, Guerreiro S, et al. Establishing a Link between Endothelial Cell Metabolism and Vascular Behaviour in a Type 1 Diabetes Mouse Model. Cell Physiol Biochem. 2019;52:503-516 pubmed publisher
Ni F, Yu W, Wang X, Fay M, Young K, Qiu Y, et al. Ptpn21 Controls Hematopoietic Stem Cell Homeostasis and Biomechanics. Cell Stem Cell. 2019;24:608-620.e6 pubmed publisher
Courties G, Frodermann V, Honold L, Zheng Y, Herisson F, Schloss M, et al. Glucocorticoids Regulate Bone Marrow B Lymphopoiesis After Stroke. Circ Res. 2019;124:1372-1385 pubmed publisher
Denton A, Innocentin S, Carr E, Bradford B, Lafouresse F, Mabbott N, et al. Type I interferon induces CXCL13 to support ectopic germinal center formation. J Exp Med. 2019;216:621-637 pubmed publisher
He W, Holtkamp S, Hergenhan S, Kraus K, De Juan A, Weber J, et al. Circadian Expression of Migratory Factors Establishes Lineage-Specific Signatures that Guide the Homing of Leukocyte Subsets to Tissues. Immunity. 2018;49:1175-1190.e7 pubmed publisher
Kolb R, Kluz P, Tan Z, Borcherding N, Bormann N, Vishwakarma A, et al. Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4. Oncogene. 2019;38:2351-2363 pubmed publisher
Wu F, Xu P, Chow A, Man S, Kruger J, Khan K, et al. Pre- and post-operative anti-PD-L1 plus anti-angiogenic therapies in mouse breast or renal cancer models of micro- or macro-metastatic disease. Br J Cancer. 2019;120:196-206 pubmed publisher
Welz M, Eickhoff S, Abdullah Z, Trebicka J, Gartlan K, Spicer J, et al. Perforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis. Nat Commun. 2018;9:4805 pubmed publisher
Bousquenaud M, Fico F, Solinas G, Ruegg C, Santamaria Martínez A. Obesity promotes the expansion of metastasis-initiating cells in breast cancer. Breast Cancer Res. 2018;20:104 pubmed publisher
Wang H, D Souza C, Lim X, Kostenko L, Pediongco T, Eckle S, et al. MAIT cells protect against pulmonary Legionella longbeachae infection. Nat Commun. 2018;9:3350 pubmed publisher
Himburg H, Termini C, Schlussel L, Kan J, Li M, Zhao L, et al. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration. Cell Stem Cell. 2018;23:370-381.e5 pubmed publisher
Surewaard B, Thanabalasuriar A, Zeng Z, Tkaczyk C, Cohen T, Bardoel B, et al. α-Toxin Induces Platelet Aggregation and Liver Injury during Staphylococcus aureus Sepsis. Cell Host Microbe. 2018;24:271-284.e3 pubmed publisher
Maeda K, Otomo K, Yoshida N, Abu Asab M, Ichinose K, Nishino T, et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. J Clin Invest. 2018;128:3445-3459 pubmed publisher
Vandoorne K, Rohde D, Kim H, Courties G, Wojtkiewicz G, Honold L, et al. Imaging the Vascular Bone Marrow Niche During Inflammatory Stress. Circ Res. 2018;: pubmed publisher
Baron C, Kester L, Klaus A, Boisset J, Thambyrajah R, Yvernogeau L, et al. Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta. Nat Commun. 2018;9:2517 pubmed publisher
Xu C, Gao X, Wei Q, Nakahara F, Zimmerman S, Mar J, et al. Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow. Nat Commun. 2018;9:2449 pubmed publisher
Winter C, Silvestre Roig C, Ortega Gomez A, Lemnitzer P, Poelman H, Schumski A, et al. Chrono-pharmacological Targeting of the CCL2-CCR2 Axis Ameliorates Atherosclerosis. Cell Metab. 2018;28:175-182.e5 pubmed publisher
Zimmerli D, Cecconi V, Valenta T, Hausmann G, Cantù C, Restivo G, et al. WNT ligands control initiation and progression of human papillomavirus-driven squamous cell carcinoma. Oncogene. 2018;37:3753-3762 pubmed publisher
Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
Hirata Y, Furuhashi K, Ishii H, Li H, Pinho S, Ding L, et al. CD150high Bone Marrow Tregs Maintain Hematopoietic Stem Cell Quiescence and Immune Privilege via Adenosine. Cell Stem Cell. 2018;22:445-453.e5 pubmed publisher
Pinho S, Marchand T, Yang E, Wei Q, Nerlov C, Frenette P. Lineage-Biased Hematopoietic Stem Cells Are Regulated by Distinct Niches. Dev Cell. 2018;44:634-641.e4 pubmed publisher
Kim I, Kim K, Lee E, Oh D, Park C, Park S, et al. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality. J Exp Med. 2018;215:963-983 pubmed publisher
Böttcher J, Bonavita E, Chakravarty P, Blees H, Cabeza Cabrerizo M, Sammicheli S, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control. Cell. 2018;172:1022-1037.e14 pubmed publisher
Shimba A, Cui G, Tani ichi S, Ogawa M, Abe S, Okazaki F, et al. Glucocorticoids Drive Diurnal Oscillations in T Cell Distribution and Responses by Inducing Interleukin-7 Receptor and CXCR4. Immunity. 2018;48:286-298.e6 pubmed publisher
Lee E, Gillrie M, Li L, Arnason J, Kim J, Babes L, et al. Leukotriene B4-Mediated Neutrophil Recruitment Causes Pulmonary Capillaritis during Lethal Fungal Sepsis. Cell Host Microbe. 2018;23:121-133.e4 pubmed publisher
Engblom C, Pfirschke C, Zilionis R, da Silva Martins J, Bos S, Courties G, et al. Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils. Science. 2017;358: pubmed publisher
Perry H, Huang L, Wilson R, Bajwa A, Sesaki H, Yan Z, et al. Dynamin-Related Protein 1 Deficiency Promotes Recovery from AKI. J Am Soc Nephrol. 2018;29:194-206 pubmed publisher
Kanemaru H, Yamane F, Fukushima K, Matsuki T, Kawasaki T, Ebina I, et al. Antitumor effect of Batf2 through IL-12 p40 up-regulation in tumor-associated macrophages. Proc Natl Acad Sci U S A. 2017;114:E7331-E7340 pubmed publisher
Thiriot A, Perdomo C, Cheng G, Novitzky Basso I, McArdle S, Kishimoto J, et al. Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues. BMC Biol. 2017;15:45 pubmed publisher
Schneider R, Mullally A, Dugourd A, Peisker F, Hoogenboezem R, van Strien P, et al. Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target. Cell Stem Cell. 2017;20:785-800.e8 pubmed publisher
Kammertoens T, Friese C, Arina A, Idel C, Briesemeister D, Rothe M, et al. Tumour ischaemia by interferon-? resembles physiological blood vessel regression. Nature. 2017;545:98-102 pubmed publisher
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product information
Antigen :
CD31
Apps. Abbrev. :
FC, 3D IHC, SB
Cat # :
102516
Clone :
MEC13.3
Item :
Alexa Fluor® 647 anti-mouse CD31
Isotype :
Rat IgG2a, κ
Other Names :
PECAM-1, EndoCAM
Size :
100 μg
Price (USD) :
229 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A647
Immunogen :
Polyoma middle T transformed EC line tEnd.1
Application Notes :
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.^8 Additional reported applications (in the relevant formats) include: immunoprecipitation^1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^12,13.Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA