product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Alexa Fluor® 488 anti-mouse CD31
catalog :
102514
quantity :
100 μg
price :
229 USD
clonality :
monoclonal
host :
rat
conjugate :
AF488
clone name :
MEC13.3
reactivity :
mouse
application :
immunohistochemistry, flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 60
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1c
Duan J, Zhou Z, Ruan B, Fang Z, Ding J, Liu J, et al. Notch-Regulated c-Kit-Positive Liver Sinusoidal Endothelial Cells Contribute to Liver Zonation and Regeneration. Cell Mol Gastroenterol Hepatol. 2022;13:1741-1756 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5e
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5f
Thanabalasuriar A, Chiang A, Morehouse C, Camara M, Hawkins S, Keller A, et al. PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Sci Adv. 2021;7: pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 2a
Chelvanambi M, Fecek R, Taylor J, Storkus W. STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment. J Immunother Cancer. 2021;9: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Xanthis I, Souilhol C, Serbanovic Canic J, Roddie H, Kalli A, Fragiadaki M, et al. β1 integrin is a sensor of blood flow direction. J Cell Sci. 2019;132: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Cunin P, Bouslama R, Machlus K, Martínez Bonet M, Lee P, Wactor A, et al. Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig ev1b
Gurevich D, Severn C, Twomey C, Greenhough A, Cash J, Toye A, et al. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1f
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Singh P, Hoggatt J, Kamocka M, Mohammad K, Saunders M, Li H, et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest. 2017;127:4527-4540 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
Chen W, Cao Z, Truong L, Sugaya S, Panjwani N. Fingerprinting of galectins in normal, P. aeruginosa-infected, and chemically burned mouse corneas. Invest Ophthalmol Vis Sci. 2015;56:515-25 pubmed publisher
Limbad C, Doi R, McGirr J, Ciotlos S, Perez K, Clayton Z, et al. Senolysis induced by 25-hydroxycholesterol targets CRYAB in multiple cell types. iScience. 2022;25:103848 pubmed publisher
Mana M, Hussey A, Tzouanas C, Imada S, Barrera Millan Y, Bahceci D, et al. High-fat diet-activated fatty acid oxidation mediates intestinal stemness and tumorigenicity. Cell Rep. 2021;35:109212 pubmed publisher
Zhao G, Weiner A, Neupauer K, De Mello Costa M, Palashikar G, Adams Tzivelekidis S, et al. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2. Sci Adv. 2020;6: pubmed publisher
Li Q, Lin J, Rosen S, Zhang T, Kazerounian S, Luo S, et al. Striated Preferentially Expressed Protein Kinase (SPEG)-Deficient Skeletal Muscles Display Fewer Satellite Cells with Reduced Proliferation and Delayed Differentiation. Am J Pathol. 2020;190:2453-2463 pubmed publisher
Konno A, Matsumoto N, Tomono Y, Okazaki S. Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures. Sci Rep. 2020;10:12613 pubmed publisher
Krausgruber T, Fortelny N, Fife Gernedl V, Senekowitsch M, Schuster L, Lercher A, et al. Structural cells are key regulators of organ-specific immune responses. Nature. 2020;583:296-302 pubmed publisher
Gomez I, Ward B, Souilhol C, Recarti C, Ariaans M, Johnston J, et al. Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium. Nat Commun. 2020;11:214 pubmed publisher
Wegner S, Uhlemann R, Boujon V, Ersoy B, Endres M, Kronenberg G, et al. Endothelial Cell-Specific Transcriptome Reveals Signature of Chronic Stress Related to Worse Outcome After Mild Transient Brain Ischemia in Mice. Mol Neurobiol. 2020;57:1446-1458 pubmed publisher
Choudhury S, Babes L, Rahn J, Ahn B, Goring K, King J, et al. Dipeptidase-1 Is an Adhesion Receptor for Neutrophil Recruitment in Lungs and Liver. Cell. 2019;178:1205-1221.e17 pubmed publisher
Amezcua Vesely M, Pallis P, Bielecki P, Low J, Zhao J, Harman C, et al. Effector TH17 Cells Give Rise to Long-Lived TRM Cells that Are Essential for an Immediate Response against Bacterial Infection. Cell. 2019;178:1176-1188.e15 pubmed publisher
Lang J, Toulmin S, Brida K, Eisenlohr L, Davidson B. Standard screening methods underreport AAV-mediated transduction and gene editing. Nat Commun. 2019;10:3415 pubmed publisher
Wuebbles R, Cruz V, Van Ry P, Barraza Flores P, Brewer P, Jones P, et al. Human Galectin-1 Improves Sarcolemma Stability and Muscle Vascularization in the mdx Mouse Model of Duchenne Muscular Dystrophy. Mol Ther Methods Clin Dev. 2019;13:145-153 pubmed publisher
Denton A, Innocentin S, Carr E, Bradford B, Lafouresse F, Mabbott N, et al. Type I interferon induces CXCL13 to support ectopic germinal center formation. J Exp Med. 2019;216:621-637 pubmed publisher
Sakurai Y, Hada T, Kato A, Hagino Y, Mizumura W, Harashima H. Effective Therapy Using a Liposomal siRNA that Targets the Tumor Vasculature in a Model Murine Breast Cancer with Lung Metastasis. Mol Ther Oncolytics. 2018;11:102-108 pubmed publisher
Kolb R, Kluz P, Tan Z, Borcherding N, Bormann N, Vishwakarma A, et al. Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4. Oncogene. 2019;38:2351-2363 pubmed publisher
Wu F, Xu P, Chow A, Man S, Kruger J, Khan K, et al. Pre- and post-operative anti-PD-L1 plus anti-angiogenic therapies in mouse breast or renal cancer models of micro- or macro-metastatic disease. Br J Cancer. 2019;120:196-206 pubmed publisher
Welz M, Eickhoff S, Abdullah Z, Trebicka J, Gartlan K, Spicer J, et al. Perforin inhibition protects from lethal endothelial damage during fulminant viral hepatitis. Nat Commun. 2018;9:4805 pubmed publisher
Boulais P, Mizoguchi T, Zimmerman S, Nakahara F, Vivié J, Mar J, et al. The Majority of CD45- Ter119- CD31- Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors. Immunity. 2018;49:627-639.e6 pubmed publisher
Bousquenaud M, Fico F, Solinas G, Ruegg C, Santamaria Martínez A. Obesity promotes the expansion of metastasis-initiating cells in breast cancer. Breast Cancer Res. 2018;20:104 pubmed publisher
Wang H, D Souza C, Lim X, Kostenko L, Pediongco T, Eckle S, et al. MAIT cells protect against pulmonary Legionella longbeachae infection. Nat Commun. 2018;9:3350 pubmed publisher
Son N, Basu D, Samovski D, Pietka T, Peche V, Willecke F, et al. Endothelial cell CD36 optimizes tissue fatty acid uptake. J Clin Invest. 2018;128:4329-4342 pubmed publisher
Bhattacharya A, Lord B, Grigoleit J, He Y, Fraser I, Campbell S, et al. Neuropsychopharmacology of JNJ-55308942: evaluation of a clinical candidate targeting P2X7 ion channels in animal models of neuroinflammation and anhedonia. Neuropsychopharmacology. 2018;43:2586-2596 pubmed publisher
Maeda K, Otomo K, Yoshida N, Abu Asab M, Ichinose K, Nishino T, et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. J Clin Invest. 2018;128:3445-3459 pubmed publisher
Vandoorne K, Rohde D, Kim H, Courties G, Wojtkiewicz G, Honold L, et al. Imaging the Vascular Bone Marrow Niche During Inflammatory Stress. Circ Res. 2018;: pubmed publisher
Xu C, Gao X, Wei Q, Nakahara F, Zimmerman S, Mar J, et al. Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow. Nat Commun. 2018;9:2449 pubmed publisher
Zimmerli D, Cecconi V, Valenta T, Hausmann G, Cantù C, Restivo G, et al. WNT ligands control initiation and progression of human papillomavirus-driven squamous cell carcinoma. Oncogene. 2018;37:3753-3762 pubmed publisher
Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
Kim I, Kim K, Lee E, Oh D, Park C, Park S, et al. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality. J Exp Med. 2018;215:963-983 pubmed publisher
Böttcher J, Bonavita E, Chakravarty P, Blees H, Cabeza Cabrerizo M, Sammicheli S, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control. Cell. 2018;172:1022-1037.e14 pubmed publisher
Engblom C, Pfirschke C, Zilionis R, da Silva Martins J, Bos S, Courties G, et al. Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils. Science. 2017;358: pubmed publisher
Kanemaru H, Yamane F, Fukushima K, Matsuki T, Kawasaki T, Ebina I, et al. Antitumor effect of Batf2 through IL-12 p40 up-regulation in tumor-associated macrophages. Proc Natl Acad Sci U S A. 2017;114:E7331-E7340 pubmed publisher
Thiriot A, Perdomo C, Cheng G, Novitzky Basso I, McArdle S, Kishimoto J, et al. Differential DARC/ACKR1 expression distinguishes venular from non-venular endothelial cells in murine tissues. BMC Biol. 2017;15:45 pubmed publisher
Kammertoens T, Friese C, Arina A, Idel C, Briesemeister D, Rothe M, et al. Tumour ischaemia by interferon-? resembles physiological blood vessel regression. Nature. 2017;545:98-102 pubmed publisher
Tian L, Goldstein A, Wang H, Ching Lo H, Sun Kim I, Welte T, et al. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming. Nature. 2017;544:250-254 pubmed publisher
Sung S, Ge Y, Dai C, Wang H, Fu S, Sharma R, et al. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice. J Immunol. 2017;198:2589-2601 pubmed publisher
Mueller A, van Velthoven C, Fukumoto K, Cheung T, Rando T. Intronic polyadenylation of PDGFR? in resident stem cells attenuates muscle fibrosis. Nature. 2016;540:276-279 pubmed publisher
Chen W, Cao Z, Sugaya S, Lopez M, Sendra V, Laver N, et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun. 2016;7:11302 pubmed publisher
Kuo P, Scofield B, Yu I, Chang F, Ganea D, Yen J. Interferon-β Modulates Inflammatory Response in Cerebral Ischemia. J Am Heart Assoc. 2016;5: pubmed publisher
Ramos Perez W, Fang V, Escalante Alcalde D, Cammer M, Schwab S. A map of the distribution of sphingosine 1-phosphate in the spleen. Nat Immunol. 2015;16:1245-52 pubmed publisher
Nacer A, Movila A, Sohet F, Girgis N, Gundra U, Loke P, et al. Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier. PLoS Pathog. 2014;10:e1004528 pubmed publisher
De Rossi G, Evans A, Kay E, Woodfin A, McKay T, Nourshargh S, et al. Shed syndecan-2 inhibits angiogenesis. J Cell Sci. 2014;127:4788-99 pubmed publisher
Giacomini C, Ferrari G, Bignami F, Rama P. Alkali burn versus suture-induced corneal neovascularization in C57BL/6 mice: an overview of two common animal models of corneal neovascularization. Exp Eye Res. 2014;121:1-4 pubmed publisher
Takeda K, Duan L, Takeda H, Fong G. Improved vascular survival and growth in the mouse model of hindlimb ischemia by a remote signaling mechanism. Am J Pathol. 2014;184:686-96 pubmed publisher
Lu Q, Li M, Zou Y, Cao T. Delivery of basic fibroblast growth factors from heparinized decellularized adipose tissue stimulates potent de novo adipogenesis. J Control Release. 2014;174:43-50 pubmed publisher
Leung V, Yun S, Botto M, Mason J, Malik T, Song W, et al. Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice. Am J Pathol. 2009;175:1757-67 pubmed publisher
Tang X, Cairns B, Cairns N, Yenari M. Apocynin improves outcome in experimental stroke with a narrow dose range. Neuroscience. 2008;154:556-62 pubmed publisher
product information
Antigen :
CD31
Apps. Abbrev. :
FC
Cat # :
102514
Clone :
MEC13.3
Item :
Alexa Fluor® 488 anti-mouse CD31
Isotype :
Rat IgG2a, κ
Other Names :
PECAM-1, EndoCAM
Size :
100 μg
Price (USD) :
229 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
A488
Immunogen :
Polyoma middle T transformed EC line tEnd.1
Application Notes :
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.^8 Additional reported applications (in the relevant formats) include: immunoprecipitation^1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^12,13.Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA