product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Biotin anti-mouse CD31
catalog :
102504
quantity :
500 μg
price :
297 USD
clonality :
monoclonal
host :
rat
conjugate :
biotin
clone name :
MEC13.3
reactivity :
mouse
application :
flow cytometry, immunohistochemistry - frozen section
more info or order :
citations: 37
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1e
Mehatre S, Roy I, Biswas A, Prit D, Schouteden S, Huelsken J, et al. Niche-Mediated Integrin Signaling Supports Steady-State Hematopoiesis in the Spleen. J Immunol. 2021;206:1549-1560 pubmed publisher
  • flow cytometry; mouse; 1:200; fig 2s2d
Laurin M, Gomez N, Levorse J, Sendoel A, Sribour M, Fuchs E. An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis. elife. 2019;8: pubmed publisher
  • flow cytometry; mouse
Kaenel P, Hahnewald S, Wotzkow C, Strange R, Andres A. Overexpression of EphB4 in the mammary epithelium shifts the differentiation pathway of progenitor cells and promotes branching activity and vascularization. Dev Growth Differ. 2014;56:255-75 pubmed publisher
Fadzeyeva E, Locatelli C, Trzaskalski N, Nguyen M, Capozzi M, Vulesevic B, et al. Pancreas-derived DPP4 is not essential for glucose homeostasis under metabolic stress. iScience. 2023;26:106748 pubmed publisher
Lv X, Liu S, Liu C, Li Y, Zhang T, Qi J, et al. TRIB3 promotes pulmonary fibrosis through inhibiting SLUG degradation by physically interacting with MDM2. Acta Pharm Sin B. 2023;13:1631-1647 pubmed publisher
Hao M, Fu R, Tai J, Tian Z, Yuan X, Chen Y, et al. S1PR1 serves as a viable drug target against pulmonary fibrosis by increasing the integrity of the endothelial barrier of the lung. Acta Pharm Sin B. 2023;13:1110-1127 pubmed publisher
Lin C, Yang Q, Guo D, Xie J, Yang Y, Chaugule S, et al. Impaired mitochondrial oxidative metabolism in skeletal progenitor cells leads to musculoskeletal disintegration. Nat Commun. 2022;13:6869 pubmed publisher
Lv X, Liu C, Liu S, Li Y, Wang W, Li K, et al. The cell cycle inhibitor P21 promotes the development of pulmonary fibrosis by suppressing lung alveolar regeneration. Acta Pharm Sin B. 2022;12:735-746 pubmed publisher
Tang Y, Zhu L, Cho J, Li X, Weiss S. Matrix remodeling controls a nuclear lamin A/C-emerin network that directs Wnt-regulated stem cell fate. Dev Cell. 2022;57:480-495.e6 pubmed publisher
Wang Z, Lv J, Yu P, Qu Y, Zhou Y, Zhou L, et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol. 2022;: pubmed publisher
Mo C, Guo J, Qin J, Zhang X, Sun Y, Wei H, et al. Single-cell transcriptomics of LepR-positive skeletal cells reveals heterogeneous stress-dependent stem and progenitor pools. EMBO J. 2022;41:e108415 pubmed publisher
Goossens E, Zhang L, De Vries M, Jukema J, Quax P, Nossent A. Cold-Inducible RNA-Binding Protein but Not Its Antisense lncRNA Is a Direct Negative Regulator of Angiogenesis In Vitro and In Vivo via Regulation of the 14q32 angiomiRs-microRNA-329-3p and microRNA-495-3p. Int J Mol Sci. 2021;22: pubmed publisher
Sitaraman S, Martin E, Na C, Zhao S, Green J, Deshmukh H, et al. Surfactant protein C mutation links postnatal type 2 cell dysfunction to adult disease. JCI Insight. 2021;6: pubmed publisher
Lv J, Wang Z, Qu Y, Zhu H, Zhu Q, Tong W, et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov. 2021;7:24 pubmed publisher
Ludwik K, Sandusky Z, Wright E, Lannigan D. FACS protocol for direct comparison of cell populations isolated from mice. STAR Protoc. 2021;2:100270 pubmed publisher
Wang Y, Mohseni M, Grauel A, Diez J, Guan W, Liang S, et al. SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms. Sci Rep. 2021;11:1399 pubmed publisher
Theilmann A, Hawke L, Hilton L, Whitford M, Cole D, MacKeil J, et al. Endothelial BMPR2 Loss Drives a Proliferative Response to BMP (Bone Morphogenetic Protein) 9 via Prolonged Canonical Signaling. Arterioscler Thromb Vasc Biol. 2020;40:2605-2618 pubmed publisher
Ray H, Corliss B, Bruce A, Kesting S, Dey P, Mansour J, et al. Myh11+ microvascular mural cells and derived mesenchymal stem cells promote retinal fibrosis. Sci Rep. 2020;10:15808 pubmed publisher
Davidson S, Efremova M, Riedel A, Mahata B, Pramanik J, Huuhtanen J, et al. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth. Cell Rep. 2020;31:107628 pubmed publisher
de Bruijn H, Mashayekhi V, Schreurs T, van Driel P, Strijkers G, van Diest P, et al. Acute cellular and vascular responses to photodynamic therapy using EGFR-targeted nanobody-photosensitizer conjugates studied with intravital optical imaging and magnetic resonance imaging. Theranostics. 2020;10:2436-2452 pubmed publisher
Corliss B, DeLalio L, Stevenson Keller T, Keller A, Keller D, Corliss B, et al. Vascular Expression of Hemoglobin Alpha in Antarctic Icefish Supports Iron Limitation as Novel Evolutionary Driver. Front Physiol. 2019;10:1389 pubmed publisher
Sitaraman S, Na C, Yang L, Filuta A, Bridges J, Weaver T. Proteasome dysfunction in alveolar type 2 epithelial cells is associated with acute respiratory distress syndrome. Sci Rep. 2019;9:12509 pubmed publisher
Fu R, Han C, Ni T, Di L, Liu L, Lv W, et al. A ZEB1/p53 signaling axis in stromal fibroblasts promotes mammary epithelial tumours. Nat Commun. 2019;10:3210 pubmed publisher
He Q, Li X, Singh K, Luo Z, Meija Cordova M, Jamalpour M, et al. The Cdh5-CreERT2 transgene causes conditional Shb gene deletion in hematopoietic cells with consequences for immune cell responses to tumors. Sci Rep. 2019;9:7548 pubmed publisher
Miao Y, Yang H, Levorse J, Yuan S, Polak L, Sribour M, et al. Adaptive Immune Resistance Emerges from Tumor-Initiating Stem Cells. Cell. 2019;177:1172-1186.e14 pubmed publisher
Hishikawa A, Hayashi K, Abe T, Kaneko M, Yokoi H, Azegami T, et al. Decreased KAT5 Expression Impairs DNA Repair and Induces Altered DNA Methylation in Kidney Podocytes. Cell Rep. 2019;26:1318-1332.e4 pubmed publisher
Zhou J, Xu J, Zhang L, Liu S, Ma Y, Wen X, et al. Combined Single-Cell Profiling of lncRNAs and Functional Screening Reveals that H19 Is Pivotal for Embryonic Hematopoietic Stem Cell Development. Cell Stem Cell. 2019;24:285-298.e5 pubmed publisher
Wang C, Tanjaya J, Shen J, Lee S, Bisht B, Pan H, et al. Peroxisome Proliferator-Activated Receptor-γ Knockdown Impairs Bone Morphogenetic Protein-2-Induced Critical-Size Bone Defect Repair. Am J Pathol. 2019;189:648-664 pubmed publisher
Fu N, Pal B, Chen Y, Jackling F, Milevskiy M, Vaillant F, et al. Foxp1 Is Indispensable for Ductal Morphogenesis and Controls the Exit of Mammary Stem Cells from Quiescence. Dev Cell. 2018;47:629-644.e8 pubmed publisher
Urner S, Planas Paz L, Hilger L, Henning C, Branopolski A, Kelly Goss M, et al. Identification of ILK as a critical regulator of VEGFR3 signalling and lymphatic vascular growth. EMBO J. 2019;38: pubmed publisher
Rodda L, Lu E, Bennett M, Sokol C, Wang X, Luther S, et al. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity. Immunity. 2018;48:1014-1028.e6 pubmed publisher
Adam R, Yang H, Ge Y, Lien W, Wang P, Zhao Y, et al. Temporal Layering of Signaling Effectors Drives Chromatin Remodeling during Hair Follicle Stem Cell Lineage Progression. Cell Stem Cell. 2018;22:398-413.e7 pubmed publisher
Duarte D, Hawkins E, Akinduro O, Ang H, De Filippo K, Kong I, et al. Inhibition of Endosteal Vascular Niche Remodeling Rescues Hematopoietic Stem Cell Loss in AML. Cell Stem Cell. 2018;22:64-77.e6 pubmed publisher
Giacomini C, Ferrari G, Bignami F, Rama P. Alkali burn versus suture-induced corneal neovascularization in C57BL/6 mice: an overview of two common animal models of corneal neovascularization. Exp Eye Res. 2014;121:1-4 pubmed publisher
Suzuki E, Karam E, Williams S, Watson D, Gilkeson G, Zhang X. Fli-1 transcription factor affects glomerulonephritis development by regulating expression of monocyte chemoattractant protein-1 in endothelial cells in the kidney. Clin Immunol. 2012;145:201-8 pubmed publisher
Murakami A, Oshiro H, Kanzaki S, Yamaguchi A, Yamanaka S, Furuya M, et al. A novel method for isolating podocytes using magnetic activated cell sorting. Nephrol Dial Transplant. 2010;25:3884-90 pubmed publisher
Leung V, Yun S, Botto M, Mason J, Malik T, Song W, et al. Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice. Am J Pathol. 2009;175:1757-67 pubmed publisher
product information
Antigen :
CD31
Apps. Abbrev. :
FC
Cat # :
102504
Clone :
MEC13.3
Item :
Biotin anti-mouse CD31
Isotype :
Rat IgG2a, κ
Other Names :
PECAM-1, EndoCAM
Size :
500 μg
Price (USD) :
297 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
BIOTIN
Immunogen :
Polyoma middle T transformed EC line tEnd.1
Application Notes :
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.^8 Additional reported applications (in the relevant formats) include: immunoprecipitation^1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^12,13.Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA