product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Purified anti-mouse CD31
catalog :
102501
quantity :
50 μg
price :
71 USD
clonality :
monoclonal
host :
rat
conjugate :
nonconjugated
clone name :
MEC13.3
reactivity :
mouse
application :
immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 136
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig 1c
Duan J, Zhou Z, Ruan B, Fang Z, Ding J, Liu J, et al. Notch-Regulated c-Kit-Positive Liver Sinusoidal Endothelial Cells Contribute to Liver Zonation and Regeneration. Cell Mol Gastroenterol Hepatol. 2022;13:1741-1756 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2a
Uhl B, Braun C, Dominik J, Luft J, Canis M, Reichel C. A Novel Experimental Approach for In Vivo Analyses of the Salivary Gland Microvasculature. Front Immunol. 2020;11:604470 pubmed publisher
  • flow cytometry; mouse; 1:100; loading ...; fig 5e
  • immunohistochemistry; mouse; 1:100; loading ...; fig 5f
Thanabalasuriar A, Chiang A, Morehouse C, Camara M, Hawkins S, Keller A, et al. PD-L1+ neutrophils contribute to injury-induced infection susceptibility. Sci Adv. 2021;7: pubmed publisher
  • immunohistochemistry; mouse; 1:50; fig 2a
Chelvanambi M, Fecek R, Taylor J, Storkus W. STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment. J Immunother Cancer. 2021;9: pubmed publisher
  • flow cytometry; mouse; 1:1000; loading ...; fig s1-1a
Tremblay M, Viala S, Shafer M, Graham Paquin A, Liu C, Bouchard M. Regulation of stem/progenitor cell maintenance by BMP5 in prostate homeostasis and cancer initiation. elife. 2020;9: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 6a
Xanthis I, Souilhol C, Serbanovic Canic J, Roddie H, Kalli A, Fragiadaki M, et al. β1 integrin is a sensor of blood flow direction. J Cell Sci. 2019;132: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 1a
Cunin P, Bouslama R, Machlus K, Martínez Bonet M, Lee P, Wactor A, et al. Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets. elife. 2019;8: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig ev1b
Gurevich D, Severn C, Twomey C, Greenhough A, Cash J, Toye A, et al. Live imaging of wound angiogenesis reveals macrophage orchestrated vessel sprouting and regression. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1f
Stremmel C, Schuchert R, Wagner F, Thaler R, Weinberger T, Pick R, et al. Yolk sac macrophage progenitors traffic to the embryo during defined stages of development. Nat Commun. 2018;9:75 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1a
Guarnerio J, Mendez L, Asada N, Menon A, Fung J, Berry K, et al. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche. Nat Commun. 2018;9:66 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 2c
Singh P, Hoggatt J, Kamocka M, Mohammad K, Saunders M, Li H, et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest. 2017;127:4527-4540 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig s8c
Dong L, Yu W, Zheng H, Loh M, Bunting S, Pauly M, et al. Leukaemogenic effects of Ptpn11 activating mutations in the stem cell microenvironment. Nature. 2016;539:304-308 pubmed publisher
  • flow cytometry; mouse; 1:300; loading ...; fig 1b
Riedel A, Shorthouse D, Haas L, Hall B, Shields J. Tumor-induced stromal reprogramming drives lymph node transformation. Nat Immunol. 2016;17:1118-27 pubmed publisher
  • flow cytometry; mouse; loading ...; fig s3a
Quantius J, Schmoldt C, Vazquez Armendariz A, Becker C, El Agha E, Wilhelm J, et al. Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair. PLoS Pathog. 2016;12:e1005544 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
Chen W, Cao Z, Sugaya S, Lopez M, Sendra V, Laver N, et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun. 2016;7:11302 pubmed publisher
  • flow cytometry; mouse; fig s4
Beyaz S, Mana M, Roper J, Kedrin D, Saadatpour A, Hong S, et al. High-fat diet enhances stemness and tumorigenicity of intestinal progenitors. Nature. 2016;531:53-8 pubmed publisher
  • blocking or activating experiments; mouse; fig 2
Guidotti L, Inverso D, Sironi L, Di Lucia P, Fioravanti J, Ganzer L, et al. Immunosurveillance of the liver by intravascular effector CD8(+) T cells. Cell. 2015;161:486-500 pubmed publisher
  • flow cytometry; mouse; loading ...; fig 1
Woods S, Waite A, O Dea K, Halford P, Takata M, Wilson M. Kinetic profiling of in vivo lung cellular inflammatory responses to mechanical ventilation. Am J Physiol Lung Cell Mol Physiol. 2015;308:L912-21 pubmed publisher
  • flow cytometry; mouse; fig 2
Richardson G, Lannigan J, Macara I. Does FACS perturb gene expression?. Cytometry A. 2015;87:166-75 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 1
Chen W, Cao Z, Truong L, Sugaya S, Panjwani N. Fingerprinting of galectins in normal, P. aeruginosa-infected, and chemically burned mouse corneas. Invest Ophthalmol Vis Sci. 2015;56:515-25 pubmed publisher
  • immunocytochemistry; mouse
Nacer A, Movila A, Sohet F, Girgis N, Gundra U, Loke P, et al. Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier. PLoS Pathog. 2014;10:e1004528 pubmed publisher
  • immunohistochemistry - frozen section; mouse; fig 6
Ito A, Fujimura M, Niizuma K, Kanoke A, Sakata H, Morita Fujimura Y, et al. Enhanced post-ischemic angiogenesis in mice lacking RNF213; a susceptibility gene for moyamoya disease. Brain Res. 2015;1594:310-20 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2
Bajwa A, Rosin D, Chrościcki P, Lee S, Dondeti K, Ye H, et al. Sphingosine 1-phosphate receptor-1 enhances mitochondrial function and reduces cisplatin-induced tubule injury. J Am Soc Nephrol. 2015;26:908-25 pubmed publisher
  • flow cytometry; mouse; 1:60
Lafkas D, Rodilla V, Huyghe M, Mourao L, Kiaris H, Fre S. Notch3 marks clonogenic mammary luminal progenitor cells in vivo. J Cell Biol. 2013;203:47-56 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Qian H, Badaloni A, Chiara F, Stjernberg J, Polisetti N, Nihlberg K, et al. Molecular characterization of prospectively isolated multipotent mesenchymal progenitors provides new insight into the cellular identity of mesenchymal stem cells in mouse bone marrow. Mol Cell Biol. 2013;33:661-77 pubmed publisher
Song J, Deshpande T, Zhang X, Hannocks M, Lycke N, Cardell S, et al. The extracellular matrix of lymph node reticular fibers modulates follicle border interactions and germinal center formation. iScience. 2023;26:106753 pubmed publisher
Zanini F, Che X, Knutsen C, Liu M, Suresh N, Domingo Gonzalez R, et al. Developmental diversity and unique sensitivity to injury of lung endothelial subtypes during postnatal growth. iScience. 2023;26:106097 pubmed publisher
Citro A, Neroni A, Pignatelli C, Campo F, Policardi M, Monieri M, et al. Directed self-assembly of a xenogeneic vascularized endocrine pancreas for type 1 diabetes. Nat Commun. 2023;14:878 pubmed publisher
Russo S, Cathomas F, Lin H, CHAN K, Li L, Cuttoli R, et al. Peripheral immune-derived matrix metalloproteinase promotes stress susceptibility. Res Sq. 2023;: pubmed publisher
Bai Q, Wang X, Yan H, Wen L, Zhou Z, Ye Y, et al. Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling. J Pers Med. 2023;13: pubmed publisher
Mahmud N, Eisner C, Purushothaman S, Storer M, Kaplan D, Miller F. Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration. Cell Rep. 2022;41:111853 pubmed publisher
Moore A, Vivanco Gonzalez N, Plummer K, Mitchel O, Kaur H, Rivera M, et al. Gestationally dependent immune organization at the maternal-fetal interface. Cell Rep. 2022;41:111651 pubmed publisher
Ngwa V, Edwards D, Hwang Y, Karno B, Wang X, Yan C, et al. Loss of vascular endothelial glutaminase inhibits tumor growth and metastasis, and increases sensitivity to chemotherapy. Cancer Res Commun. 2022;2:694-705 pubmed publisher
Zhou Z, Jing Y, Niu Y, Chang T, Sun J, Guo C, et al. Distinguished Functions of Microglia in the Two Stages of Oxygen-Induced Retinopathy: A Novel Target in the Treatment of Ischemic Retinopathy. Life (Basel). 2022;12: pubmed publisher
Georgieva J, Katt M, Ye Z, Umlauf B, Wenthur C, Shusta E. The 46.1 Antibody Mediates Neurotensin Uptake into the CNS and the Effects Depend on the Route of Intravenous Administration. Pharmaceutics. 2022;14: pubmed publisher
Fuglerud B, Drissler S, Lotto J, Stephan T, Thakur A, Cullum R, et al. SOX9 reprograms endothelial cells by altering the chromatin landscape. Nucleic Acids Res. 2022;50:8547-8565 pubmed publisher
Hansmeier N, B xfc schlen I, Behncke R, Ulferts S, Bisoendial R, H xe4 gerling R. 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM). Int J Mol Sci. 2022;23: pubmed publisher
Fu A, Yao B, Dong T, Chen Y, Yao J, Liu Y, et al. Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer. Cell. 2022;185:1356-1372.e26 pubmed publisher
Zakharova I, Saaya S, Shevchenko A, Stupnikova A, Zhiven M, Laktionov P, et al. Mitomycin-Treated Endothelial and Smooth Muscle Cells Suitable for Safe Tissue Engineering Approaches. Front Bioeng Biotechnol. 2022;10:772981 pubmed publisher
Wang X, Shelton S, Bordieanu B, Frank A, Yi Y, Venigalla S, et al. Scinderin promotes fusion of electron transport chain dysfunctional muscle stem cells with myofibers. Nat Aging. 2022;2:155-169 pubmed publisher
Maul A, Huebner A, Strenzke N, Moser T, Rubsamen R, Jovanovic S, et al. The Cl--channel TMEM16A is involved in the generation of cochlear Ca2+ waves and promotes the refinement of auditory brainstem networks in mice. elife. 2022;11: pubmed publisher
Baluk P, McDonald D. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts. Methods Mol Biol. 2022;2441:115-134 pubmed publisher
Figueiredo C, Steffen J, Morton L, Arumugam S, Liesenfeld O, Deli M, et al. Immune response and pathogen invasion at the choroid plexus in the onset of cerebral toxoplasmosis. J Neuroinflammation. 2022;19:17 pubmed publisher
Wang Z, Lv J, Yu P, Qu Y, Zhou Y, Zhou L, et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol. 2022;: pubmed publisher
Ye P, Fu Z, Chung J, Cao X, Ko H, Tian X, et al. Endothelial Agrin Is Dispensable for Normal and Tumor Angiogenesis. Front Cardiovasc Med. 2021;8:810477 pubmed publisher
Heil J, Olsavszky V, Busch K, Klapproth K, de la Torre C, Sticht C, et al. Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation. Nat Commun. 2021;12:6963 pubmed publisher
Opzoomer J, Anstee J, Dean I, Hill E, Bouybayoune I, Caron J, et al. Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression. Sci Adv. 2021;7:eabg9518 pubmed publisher
Koch P, Sandorski K, Heil J, Schmid C, K xfc rschner S, Hoffmann J, et al. Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation. Front Physiol. 2021;12:722394 pubmed publisher
Fierle J, Brioschi M, de Tiani M, Wetterwald L, Atsaves V, Abram Saliba J, et al. Soluble trivalent engagers redirect cytolytic T cell activity toward tumor endothelial marker 1. Cell Rep Med. 2021;2:100362 pubmed publisher
Arts J, Mahlandt E, Grönloh M, Schimmel L, Noordstra I, Gordon E, et al. Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration. elife. 2021;10: pubmed publisher
Cardoso F, Klein Wolterink R, Godinho Silva C, Domingues R, Ribeiro H, da Silva J, et al. Neuro-mesenchymal units control ILC2 and obesity via a brain-adipose circuit. Nature. 2021;597:410-414 pubmed publisher
Matsumoto A, Takahashi Y, Ogata K, Kitamura S, Nakagawa N, Yamamoto A, et al. Phosphatidylserine-deficient small extracellular vesicle is a major somatic cell-derived sEV subpopulation in blood. iScience. 2021;24:102839 pubmed publisher
Reglero Real N, P xe9 rez Guti xe9 rrez L, Yoshimura A, Rolas L, Garrido Mesa J, Barkaway A, et al. Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation. Immunity. 2021;54:1989-2004.e9 pubmed publisher
Hutton C, Heider F, Blanco Gómez A, Banyard A, Kononov A, Zhang X, et al. Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity. Cancer Cell. 2021;: pubmed publisher
Goga A, Yagabasan B, Herrmanns K, Godbersen S, Silva P, Denzler R, et al. miR-802 regulates Paneth cell function and enterocyte differentiation in the mouse small intestine. Nat Commun. 2021;12:3339 pubmed publisher
Barcia Durán J, Lu T, Houghton S, Geng F, Schreiner R, Xiang J, et al. Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice. Commun Biol. 2021;4:406 pubmed publisher
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Kim J, Kolesnikov M, Peled Hajaj S, Scheyltjens I, Xia Y, Trzebanski S, et al. A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages. Immunity. 2021;54:176-190.e7 pubmed publisher
Sun W, Khare P, Wang X, Challa D, Greenberg B, Ober R, et al. Selective Depletion of Antigen-Specific Antibodies for the Treatment of Demyelinating Disease. Mol Ther. 2021;29:1312-1323 pubmed publisher
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Baluk P, Naikawadi R, Kim S, Rodríguez F, Choi D, Hong Y, et al. Lymphatic Proliferation Ameliorates Pulmonary Fibrosis after Lung Injury. Am J Pathol. 2020;190:2355-2375 pubmed publisher
Crosse E, Gordon Keylock S, Rybtsov S, Binagui Casas A, Felchle H, Nnadi N, et al. Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development. Cell Stem Cell. 2020;27:822-839.e8 pubmed publisher
Yang X, Zhong D, Gao W, Liao Z, Chen Y, Zhang S, et al. Conditional ablation of MAPK7 expression in chondrocytes impairs endochondral bone formation in limbs and adaptation of chondrocytes to hypoxia. Cell Biosci. 2020;10:103 pubmed publisher
Andreev D, Liu M, Weidner D, Kachler K, Faas M, Grüneboom A, et al. Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin. J Clin Invest. 2020;130:4811-4830 pubmed publisher
Dooley J, Lagou V, Goveia J, Ulrich A, Rohlenova K, Heirman N, et al. Heterogeneous Effects of Calorie Content and Nutritional Components Underlie Dietary Influence on Pancreatic Cancer Susceptibility. Cell Rep. 2020;32:107880 pubmed publisher
Hauptmann J, Johann L, Marini F, Kitic M, Colombo E, Mufazalov I, et al. Interleukin-1 promotes autoimmune neuroinflammation by suppressing endothelial heme oxygenase-1 at the blood-brain barrier. Acta Neuropathol. 2020;140:549-567 pubmed publisher
Takahashi K, Akatsu Y, Podyma Inoue K, Matsumoto T, Takahashi H, Yoshimatsu Y, et al. Targeting all transforming growth factor-β isoforms with an Fc chimeric receptor impairs tumor growth and angiogenesis of oral squamous cell cancer. J Biol Chem. 2020;295:12559-12572 pubmed publisher
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Davidson S, Efremova M, Riedel A, Mahata B, Pramanik J, Huuhtanen J, et al. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth. Cell Rep. 2020;31:107628 pubmed publisher
Na Y, Kwon J, Kim D, Chung H, Song J, Jung D, et al. Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages. Cell Rep. 2020;31:107643 pubmed publisher
Shiuan E, Inala A, Wang S, Song W, Youngblood V, Chen J, et al. Host deficiency in ephrin-A1 inhibits breast cancer metastasis. F1000Res. 2020;9:217 pubmed publisher
Lei L, Huang Z, Feng J, Huang Z, Tao Y, Hu X, et al. Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3. Stem Cell Res Ther. 2020;11:137 pubmed publisher
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Lee C, Liu M, Agha O, Kim H, Feeley B, Liu X. Beige FAPs Transplantation Improves Muscle Quality and Shoulder Function After Massive Rotator Cuff Tears. J Orthop Res. 2020;38:1159-1166 pubmed publisher
Lee C, Liu M, Agha O, Kim H, Liu X, Feeley B. Beige fibro-adipogenic progenitor transplantation reduces muscle degeneration and improves function in a mouse model of delayed repair of rotator cuff tears. J Shoulder Elbow Surg. 2020;29:719-727 pubmed publisher
Lang J, Toulmin S, Brida K, Eisenlohr L, Davidson B. Standard screening methods underreport AAV-mediated transduction and gene editing. Nat Commun. 2019;10:3415 pubmed publisher
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Wang H, D Souza C, Lim X, Kostenko L, Pediongco T, Eckle S, et al. MAIT cells protect against pulmonary Legionella longbeachae infection. Nat Commun. 2018;9:3350 pubmed publisher
Brenner J, Pan D, Myerson J, Marcos Contreras O, Villa C, Patel P, et al. Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs by orders of magnitude. Nat Commun. 2018;9:2684 pubmed publisher
Maeda K, Otomo K, Yoshida N, Abu Asab M, Ichinose K, Nishino T, et al. CaMK4 compromises podocyte function in autoimmune and nonautoimmune kidney disease. J Clin Invest. 2018;128:3445-3459 pubmed publisher
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Sun J, Chang T, Li M, Sun L, Yan X, Yang Z, et al. SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization. Angiogenesis. 2018;21:635-652 pubmed publisher
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Das A, Huang G, Bonkowski M, Longchamp A, Li C, Schultz M, et al. Impairment of an Endothelial NAD+-H2S Signaling Network Is a Reversible Cause of Vascular Aging. Cell. 2018;173:74-89.e20 pubmed publisher
Verbiest T, Finnon R, Brown N, Cruz Garcia L, Finnon P, O Brien G, et al. Tracking preleukemic cells in vivo to reveal the sequence of molecular events in radiation leukemogenesis. Leukemia. 2018;32:1435-1444 pubmed publisher
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, et al. Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget. 2018;9:11209-11226 pubmed publisher
Kim I, Kim K, Lee E, Oh D, Park C, Park S, et al. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality. J Exp Med. 2018;215:963-983 pubmed publisher
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Song A, Zhu L, Gorantla G, Berdysz O, Amici S, Guerau de Arellano M, et al. Salient type 1 interleukin 1 receptor expression in peripheral non-immune cells. Sci Rep. 2018;8:723 pubmed publisher
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product information
Antigen :
CD31
Apps. Abbrev. :
FC, IP, Block, IHC-F
Cat # :
102501
Clone :
MEC13.3
Item :
Purified anti-mouse CD31
Isotype :
Rat IgG2a, κ
Other Names :
PECAM-1, EndoCAM
Size :
50 μg
Price (USD) :
71 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Rat
Conjugate/Tag/Label :
PURE
Immunogen :
Polyoma middle T transformed EC line tEnd.1
Application Notes :
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.^8 Additional reported applications (in the relevant formats) include: immunoprecipitation^1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions^1-4, immunohistochemical staining^1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^12,13.Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530;).;
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA