product summary
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company name :
BioLegend
other brands :
Babco, Signet, Sternberger Monoclonals, Senetek, Covance
product type :
antibody
product name :
Ultra-LEAF™ Purified anti-mouse CD28
catalog :
102115
quantity :
100 μg
price :
100 USD
clonality :
monoclonal
host :
Syrian golden hamster
conjugate :
nonconjugated
clone name :
37.51
reactivity :
human, mouse
application :
ELISA, neutralization, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments
more info or order :
citations: 30
Published Application/Species/Sample/DilutionReference
  • blocking or activating experiments; mouse; 1 ug/ml; loading ...; fig 1h
Chen H, Cong X, Wu C, Wu X, Wang J, Mao K, et al. Intratumoral delivery of CCL25 enhances immunotherapy against triple-negative breast cancer by recruiting CCR9+ T cells. Sci Adv. 2020;6:eaax4690 pubmed publisher
  • blocking or activating experiments; mouse; 1-10 ug/ml; fig 2a
Krovi S, Kappler J, Marrack P, Gapin L. Inherent reactivity of unselected TCR repertoires to peptide-MHC molecules. Proc Natl Acad Sci U S A. 2019;116:22252-22261 pubmed publisher
  • blocking or activating experiments; mouse; 0.5 ug/ml; loading ...; fig 1a
Rasoulouniriana D, Santana Magal N, Gutwillig A, Farhat Younis L, Wine Y, Saperia C, et al. A distinct subset of FcγRI-expressing Th1 cells exert antibody-mediated cytotoxic activity. J Clin Invest. 2019;129:4151-4164 pubmed publisher
  • flow cytometry; mouse; fig 1b
Humeniuk P, Geiselhart S, Battin C, Webb T, Steinberger P, Paster W, et al. Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor. Sci Rep. 2019;9:7426 pubmed publisher
  • blocking or activating experiments; mouse; loading ...; fig 5a
Fu G, Xu Q, Qiu Y, Jin X, Xu T, Dong S, et al. Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med. 2017;214:1453-1469 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml; loading ...; fig 3b
González Pérez G, Lamousé Smith E. Gastrointestinal Microbiome Dysbiosis in Infant Mice Alters Peripheral CD8+ T Cell Receptor Signaling. Front Immunol. 2017;8:265 pubmed publisher
  • blocking or activating experiments; mouse; fig 5c
Klein J, Moses K, Zelinskyy G, Sody S, Buer J, Lang S, et al. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth. Nat Commun. 2017;8:14600 pubmed publisher
  • flow cytometry; mouse; 5 ug/ml; loading ...; fig 4A
Troegeler A, Mercier I, Cougoule C, Pietretti D, Colom A, Duval C, et al. C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells. Proc Natl Acad Sci U S A. 2017;114:E540-E549 pubmed publisher
  • blocking or activating experiments; mouse; 5 ug/ml
Bieber K, Witte M, Sun S, Hundt J, Kalies K, Dräger S, et al. T cells mediate autoantibody-induced cutaneous inflammation and blistering in epidermolysis bullosa acquisita. Sci Rep. 2016;6:38357 pubmed publisher
  • ELISA; mouse; loading ...
Cecchinato V, Bernasconi E, Speck R, Proietti M, Sauermann U, D Agostino G, et al. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization. J Immunol. 2017;198:184-195 pubmed
  • blocking or activating experiments; mouse; loading ...; fig 5a
Ishikura S, Tsunoda T, Nakabayashi K, Doi K, Koyanagi M, Hayashi K, et al. Molecular mechanisms of transcriptional regulation by the nuclear zinc-finger protein Zfat in T cells. Biochim Biophys Acta. 2016;1859:1398-1410 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml; loading ...; fig 2a
Contreras F, Prado C, Gonzalez H, Franz D, Osorio Barrios F, Osorio F, et al. Dopamine Receptor D3 Signaling on CD4+ T Cells Favors Th1- and Th17-Mediated Immunity. J Immunol. 2016;196:4143-9 pubmed publisher
  • blocking or activating experiments; mouse; fig 3
Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
  • blocking or activating experiments; mouse
Hwang S, Jang S, Kim M, Kim L, Kim B, Kim H, et al. YY1 inhibits differentiation and function of regulatory T cells by blocking Foxp3 expression and activity. Nat Commun. 2016;7:10789 pubmed publisher
  • blocking or activating experiments; human; fig 1
McCully M, Collins P, Hughes T, Thomas C, Billen J, O Donnell V, et al. Skin Metabolites Define a New Paradigm in the Localization of Skin Tropic Memory T Cells. J Immunol. 2015;195:96-104 pubmed publisher
  • immunoprecipitation; mouse
Richardson E, Shukla S, Sweet D, Wearsch P, Tsichlis P, Boom W, et al. Toll-like receptor 2-dependent extracellular signal-regulated kinase signaling in Mycobacterium tuberculosis-infected macrophages drives anti-inflammatory responses and inhibits Th1 polarization of responding T cells. Infect Immun. 2015;83:2242-54 pubmed publisher
  • blocking or activating experiments; mouse; 2 ug/ml
Rao E, Zhang Y, Zhu G, Hao J, Persson X, Egilmez N, et al. Deficiency of AMPK in CD8+ T cells suppresses their anti-tumor function by inducing protein phosphatase-mediated cell death. Oncotarget. 2015;6:7944-58 pubmed
  • blocking or activating experiments; mouse; 20 ug/ml; fig 1
Fos C, Bécart S, Canonigo Balancio A, Boehning D, Altman A. Association of the EF-hand and PH domains of the guanine nucleotide exchange factor SLAT with IP₃ receptor 1 promotes Ca²⁺ signaling in T cells. Sci Signal. 2014;7:ra93 pubmed publisher
  • blocking or activating experiments; mouse; 0.5 ug/ml
Koga T, Hedrich C, Mizui M, Yoshida N, Otomo K, Lieberman L, et al. CaMK4-dependent activation of AKT/mTOR and CREM-? underlies autoimmunity-associated Th17 imbalance. J Clin Invest. 2014;124:2234-45 pubmed publisher
  • blocking or activating experiments; mouse
Nancy P, Tagliani E, Tay C, Asp P, Levy D, Erlebacher A. Chemokine gene silencing in decidual stromal cells limits T cell access to the maternal-fetal interface. Science. 2012;336:1317-21 pubmed publisher
Bromley S, Akbaba H, Mani V, Mora Buch R, Chasse A, Sama A, et al. CD49a Regulates Cutaneous Resident Memory CD8+ T Cell Persistence and Response. Cell Rep. 2020;32:108085 pubmed publisher
Zhang C, Yue C, Herrmann A, Song J, Egelston C, Wang T, et al. STAT3 Activation-Induced Fatty Acid Oxidation in CD8+ T Effector Cells Is Critical for Obesity-Promoted Breast Tumor Growth. Cell Metab. 2020;31:148-161.e5 pubmed publisher
He Y, Schreiber K, Wolf S, Wen F, Steentoft C, Zerweck J, et al. Multiple cancer-specific antigens are targeted by a chimeric antigen receptor on a single cancer cell. JCI Insight. 2019;4: pubmed publisher
Garo L, Ajay A, Fujiwara M, Beynon V, Kuhn C, Gabriely G, et al. Smad7 Controls Immunoregulatory PDL2/1-PD1 Signaling in Intestinal Inflammation and Autoimmunity. Cell Rep. 2019;28:3353-3366.e5 pubmed publisher
Dong M, Wang G, Chow R, Ye L, Zhu L, Dai X, et al. Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells. Cell. 2019;178:1189-1204.e23 pubmed publisher
Wu J, Ma S, Sandhoff R, Ming Y, Hotz Wagenblatt A, Timmerman V, et al. Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8+ T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 2019;50:1218-1231.e5 pubmed publisher
Sekiya T, Hibino S, Saeki K, Kanamori M, Takaki S, Yoshimura A. Nr4a Receptors Regulate Development and Death of Labile Treg Precursors to Prevent Generation of Pathogenic Self-Reactive Cells. Cell Rep. 2018;24:1627-1638.e6 pubmed publisher
Yuan X, Yang B, Dong Y, Yamamura A, Fu W. CRIg, a tissue-resident macrophage specific immune checkpoint molecule, promotes immunological tolerance in NOD mice, via a dual role in effector and regulatory T cells. elife. 2017;6: pubmed publisher
Cunningham N, Hinchcliff E, Kutyavin V, Beck T, Reid W, Punt J. GSK3-mediated instability of tubulin polymers is responsible for the failure of immature CD4+CD8+ thymocytes to polarize their MTOC in response to TCR stimulation. Int Immunol. 2011;23:693-700 pubmed publisher
Milpied P, Massot B, Renand A, Diem S, Herbelin A, Leite de Moraes M, et al. IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression. Blood. 2011;118:2993-3002 pubmed publisher
product information
Antigen :
CD28
Apps. Abbrev. :
Flow Cytometry, IP, IHC-F, Costim, Block
Cat # :
102115
Clone :
37.51
Item :
Ultra-LEAF™ Purified anti-mouse CD28
Isotype :
Syrian Hamster IgG
Other Names :
Tp44, T44
Size :
100 μg
Price (USD) :
100 USD
Reactivity :
Mouse
Clonality :
Monoclonal
Host :
Syrian Hamster
Conjugate/Tag/Label :
ULEAF
Immunogen :
C57BL/6 mouse T-cell lymphoma EL-4
Application Notes :
Additional reported applications (for the relevant formats) include: immunoprecipitation 1 , in vitro costimulation of T and NK cells 1 , in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity 3,4 , in vitro induction of thymocyte differentiation 2,5-9,11 , and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF purified antibody (Endotoxin 0.01 EU/ug, Azide-Free, 0.2 um filtered) (Cat. No. 102116).
more info or order :
company information
BioLegend
8999 BioLegend Way
San Diego, CA 92121
customerserv@biolegend.com
https://www.biolegend.com
headquarters: USA